Characterization of a wild-type strain of Francisella tularensis isolated from a cat.     

Thomas J Inzana  Gretchen E Glindemann  Gerald Snider  Susan Gardner  Lisa Crofton  Barbara Byrne  Joseph Harper 《Journal of veterinary diagnostic investigation》2004,16(5):374-381

Francisella tularensis type A is the primary cause of tularemia in animals and humans in North America. The majority of research on F. tularensis has been done with the attenuated live vaccine strain (LVS), which is a type B, but very few wild-type F. tularensis strains have been characterized. A gram-negative coccobacillus that was isolated in pure culture from the lungs of a cat that died after being lost for 5 days was received for identification at the Virginia-Maryland Regional College of Veterinary Medicine Teaching hospital. The isolate (strain TI0902) was not identified (or was misidentified) by commercial identification systems; however, it was identified as F. tularensis subspecies tularensis (type A) by sequencing a portion of the 16S ribosomal RNA gene. Furthermore, repetitive extragenic palindromic sequences-polymerase chain reaction amplified a 4-kb DNA fragment from TI0902 that was characteristic of F. tularensis type A but not type B. The electrophoretic profile of the lipopolysaccharide of strain TI0902 was identical to that of the LVS by Western blotting with antiserum to LVS. The protein-enriched outer membrane of strain TI0902 contained 6-8 proteins, which were similar in molecular size to those from the LVS. Electron microscopy of negatively stained and alcian blue-stained LVS and TI0902 cells showed that both strains were coccobacillary in shape and may be encapsulated. However, after mouse challenge, the TI0902 strain was clearly more virulent than the LVS strain. Results of this study indicate that the genotype and phenotype of wild-type F. tularensis type A strain TI0902 is similar, but not identical, to that of the LVS strain. Further studies will help determine whether pathogenesis and host-pathogen interactions are also similar between the 2 strains.  相似文献   

Pharmacokinetics of carfentanil and naltrexone in domestic goats (Capra hircus).     

Adrian Mutlow  Ramiro Isaza  James W Carpenter  David E Koch  Robert P Hunter 《Journal of zoo and wildlife medicine》2004,35(4):489-496

Using a crossover study design, the pharmacokinetics of carfentanil and naltrexone after i.v., i.m., and s.c. administration were determined in eight domestic goats (Capra hircus). Serial blood samples were taken up to 120 hr after carfentanil administration, and the plasma drug concentrations were determined using liquid chromatography and mass spectroscopy. All goats were immobilized with 40 microg/kg carfentanil i.m., although the resulting neurologic effects varied considerably. Plasma profiles showed rapid carfentanil absorption and a simple biphasic decline for 12-48 hr. Naltrexone given at 100 mg naltrexone/mg carfentanil 30 min after carfentanil administration produced rapid reversal of immobilization after all routes of administration. Variable fluctuations in the naltrexone plasma concentrations during the first 2.5-3.5 hr were observed, followed by a more consistent biphasic decline. The time to standing was significantly shorter after i.v. compared with s.c. naltrexone, although the time difference (1 min) had little clinical relevance. No statistically significant differences between the naltrexone pharmacokinetic parameters measured for the three routes of naltrexone administration were identified, although the recoveries after i.m. administration were, subjectively, the smoothest. The carfentanil half-life did not differ significantly in the goats given naltrexone by different routes. Although it is currently recommended that the naltrexone dose be divided into s.c. and i.v. portions, this practice does not appear to offer any benefit.  相似文献   

Endoscopic sex determination and gonadal manipulation in Gulf of Mexico sturgeon (Acipenser oxyrinchus desotoi).     

Stephen J Hernandez-Divers  Robert S Bakal  Brian H Hickson  Clarence A Rawlings  Heather G Wilson  MaryAnn Radlinsky  Sonia M Hernandez-Divers  Samuel R Dover 《Journal of zoo and wildlife medicine》2004,35(4):459-470

Seventeen Gulf of Mexico sturgeons (Acipenser oxyrinchus desotoi) underwent endoscopic sex determination, gonadal biopsy, and various reproductive surgeries as part of a conservation development plan. The fish were anesthetized with tricaine methanesulfonate (MS-222) buffered with sodium bicarbonate and maintained on a recirculating water anesthesia circuit. A 6-mm Ternamian EndoTip Cannula, placed through the ventral midline, midway between pectoral and pelvic fins, permitted the introduction of a 5-mm telescope. Swim bladder aspiration and CO2 insufflation of the coelomic cavity provided excellent observation. Second and third cannulae were placed under direct visual control, lateral and cranial or caudal to the telescope cannula. Sex determination was successfully performed in all fish; however, five of 17 sturgeons (29%) required endoscopic gonadal biopsy to confirm sex. Bilateral ovariectomy or orchidectomy was successfully performed in three males and four females. Unilateral ovariectomy and bilateral ligation of the müllerian ducts using an extracorporeal suturing technique was accomplished in an additional three females. No apparent morbidity was associated with the anesthesia or endoscopic surgery in any fish. The ability to safely perform minimally invasive reproductive surgery in fish may have important management and conservation benefits.  相似文献   

Linking Animal Abuse and Human Behaviour     

Dr  Gardner Murray 《Australian veterinary journal》2004,82(7):398-398

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Erratum to “Estimation of infection prevalence from correlated binomial samples” [Prev. Vet. Med. 64 (2004) 1–14]     

J. Condon  G. Kelly  B. Bradshaw  N. Leonard 《Preventive veterinary medicine》2004,65(3-4):239-239

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Evaluation of individual low‐dose dexamethasone suppression test patterns in naturally occurring hyperadrenocorticism in dogs          下载免费PDF全文

Michael Bennaim  Robert E. Shiel  Christopher Forde  Carmel T. Mooney 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(3):967-977

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Incidence and risk factors for neurological signs after attenuation of single congenital portosystemic shunts in 253 dogs          下载免费PDF全文

Rhiannon Strickland BVetMed  Michael S. Tivers BVSc  PhD  DipECVS  Sophie E. Adamantos BVSc  DACVECC  DECVECC  Tom R. Harcourt‐Brown MA  VetMB  DipECVN  Robert C. Fowkes BSc  PhD  Victoria J. Lipscomb MA  VetMB  DipECVS 《Veterinary surgery : VS》2018,47(6):745-755

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Knowledge,attitudes and practices towards <Emphasis Type="Italic">cystic echinococcosis</Emphasis> in livestock among selected pastoral and agro-pastoral communities in Uganda     

Leonard Omadang  Martin Chamai  Emmanuel Othieno  Andrew Okwi  Francis Olaki Inangolet  Francis Ejobi  Peter Oba  Michael Ocaido 《Tropical animal health and production》2018,50(1):11-17

A cross-sectional study was done from March 2013 to May 2014 to assess knowledge, attitudes, and practices towards cystic echinococcosis (CE) or hydatidosis among selected pastoral and agro-pastoral communities in Uganda. A structured questionnaire was administered to 381 respondents. Multivariate logistic regression analysis was done to find the relationship between knowledge about CE and factors such as age, sex, and level of education across all regions. The odds ratio and confidence interval were used to determine the difference in responses across regions. It was shown that age above 36 years was significantly (p < 0.001) associated with awareness about CE in livestock. Likewise, uneducated (p < 0.0001) and agro-pastoralists (p = 0.01) were significantly less knowledgeable than the educated and pastoralists across all regions. The overall knowledge towards CE in livestock was low 17.8% (95% CI = 14.0–21.6). Dog ownership was high and they never dewormed their freely roaming dogs. Dogs shared water with livestock. In conclusion, knowledge about CE in livestock was low across all regions. Therefore, public health education and formulation of policies towards its control by the relevant stakeholders should be done. Also, the true prevalence of CE in livestock needs to be done so that the magnitude and its public health significance are elucidated.  相似文献   

Influences of population density,temperature and latitude on the growth of invasive topmouth gudgeon Pseudorasbora parva          下载免费PDF全文

Gareth D. Davies  J. Robert Britton 《Ecology of Freshwater Fish》2015,24(1):91-98

Plasticity in life‐history traits provides advantages for introduced fish in overcoming demographic bottlenecks that would otherwise inhibit establishment. Here, the influence of population density, temperature and latitude was tested on the growth increments and growth rates of invasive populations of topmouth gudgeon Pseudorasbora parva, a small Asian cyprinid fish that is invasive across Europe. Aquaria experiments tested the roles of fish number and temperature on growth increments under a fixed food supply, pond experiments tested the role of density on growth increments, and a field study completed in England and Wales tested the influence of density and latitude on growth rates. In aquaria experiments, whilst growth increments were higher at 21 and 23 °C than at 19 and 25 °C, fish number had a greater influence on growth than temperature. Higher growth increments were produced at lower densities. In experimental ponds, growth increments were significantly higher in ponds with low densities of P. parva compared with those at elevated densities. In the field study comprising 10 wild populations across a latitudinal gradient of 4.0°N, a difference in mean air temperatures of approximately 3 °C, and estimated densities between 0.5 and 65.0 m^?2, population density was the only significant predictor of growth rates. Whilst populations at very low densities comprised of significantly faster growing individuals, there were no significant differences when densities were between 15 and 65 m^?2. Thus, invasive P. parva populations have considerable growth plasticity, especially at low densities, with this likely to be important in their ability to colonise new environments.  相似文献   

Spatial resilience of forested landscapes under climate change and management     

Melissa S. Lucash  Robert M. Scheller  Eric J. Gustafson  Brian R. Sturtevant 《Landscape Ecology》2017,32(5):953-969

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