Expression of inflammation-mediated cluster of genes as a new marker of canine mammary malignancy     

K. M. Pawłowski  A. Homa  M. Bulkowska  K. Majchrzak  T. Motyl  M. Król 《Veterinary research communications》2013,37(2):123-131

Because canine mammary tumours constitute a serious clinical problem and there are no good prognostic markers (only histopathological variables are used), the aim of the presented study was to find new malignancy markers as well as to identify intracellular pathways and biological processes characteristic for canine mammary malignancy. We compared gene expression of the most malignant mammary tumours (poorly differentiated cancers of the 3rd grade of malignancy) with less malignant tumours (well differentiated cancers of the 1st grade of malignancy). The results of our study indicated that in dogs the number of tumour-infiltrating myeloid cells or expression of myeloid-specific antigens by cancer cells is related to the cancer progression and may constitute a new marker of malignancy, however further studies in this field are required.  相似文献   

Comparative pharmacokinetics of acetylsalicylic acid and sodium salicylate in chickens and turkeys     

B. Poźniak  M. Świtała  K. Jaworski  P. Okoniewski  P. Niewiński 《British poultry science》2013,54(4):538-544

1. Pharmacokinetics of acetylsalicylic acid (ASA) and sodium salicylate (SS) were assessed following single intravenous (i.v.) and oral administration at doses of 50 mg/kg body weight to chickens and turkeys. Plasma drug concentrations were determined using high-performance liquid chromatography with ultraviolet detection and pharmacokinetic variables were calculated using a non-compartmental model.

2. The mean residence time (MRT) of salicylate (SA) after i.v. administration of SS was 6.08 ± 0.59 and 3.32 ± 0.27 h and after oral administration was 6.95 ± 0.72 and 4.55 ± 0.71 h in chickens and turkeys, respectively. The elimination half-life (T _{1/2 e}) was shorter in turkeys compared with chickens. The value of body clearance (Cl_B) was higher in turkeys than in chickens, but the apparent volume of distribution (V _ss) was similarly low in both species. The bioavailability of SS was complete and the maximal plasma concentration of SA (C _max) after oral administration was 96.93 ± 8.06 and 91.76 ± 9.64 µg/ml, respectively, in chickens and turkeys.

3. The MRT of ASA after iv administration was 0.24 ± 0.08 and 0.24 ± 0.02 h and after oral administration was 0.78 ± 0.25 and 0.59 ± 0.13 h, respectively, in chickens and turkeys. In both species, T _{1/2 e} was very short, Cl_B and V _ss were similar and markedly higher than those of salicylate. The bioavailability of unchanged ASA was low and C _max after oral administration was 6.9 ± 3.6 µg/ml in chickens and 8.6 ± 1.3 µg/ml in turkeys.  相似文献   

Spatio-temporal heterogeneity of soil microbial properties in a conventionally managed arable field     

Piotrowska-Długosz  Anna  Breza-Boruta  Barbara  Długosz  Jacek 《Journal of Soils and Sediments》2019,19(1):345-355

Journal of Soils and Sediments - Knowledge about the spatio-temporal variability of soil microbial properties is crucial in evaluating their structure-function relationship and their impact on...  相似文献   

Fruit Physical Features,Phenolic Compounds Profile and Inhibition Activities of Cranberry Cultivars (Vaccinium macrocarpon) Compared to Wild-Grown Cranberry (Vaccinium oxycoccus)     

Narwojsz  Agnieszka  Tańska  Małgorzata  Mazur  Barbara  Borowska  Eulalia Julitta 《Plant foods for human nutrition (Dordrecht, Netherlands)》2019,74(3):300-306

Plant Foods for Human Nutrition - This study analysed fruits of cranberry cultivars: Ben Lear, Bergman, Early Richard, Pilgrim and Stevens and compared them with wild-grown cranberry fruits. The...  相似文献   

Spatial and Temporal Changes of Jet Fuel Contamination in an Unconfined Sandy Aquifer     

Maciej R. Kłonowski  Gijs D. Breedveld  Per Aagaard 《Water, air, and soil pollution》2008,188(1-4):9-30

Leakage of jet fuel from an oil skimmer has resulted in soil and groundwater contamination at the fire fighting training site of the Oslo international airport. The site is located on an unconfined sandy aquifer formed by the Gardermoen ice contact delta. A strong relationship between hydrogeological settings and spatial distribution of the hydrocarbons has been observed. Leaching hydrocarbons constitute a source of dissolved BTEX and naphthalene. Concentration of the dissolved hydrocarbons, electron acceptors and metabolic by-products has revealed concomitant changes in space and in time, in response to groundwater fluctuations. Correlation between high concentrations of hydrocarbons, elevated alkalinity and depleted electron acceptors supports the hypothesis that intrinsic bioremediation has been taking place at the site. Naphthalene, however, has been found persistent under anoxic conditions. Calculations of biodegradation potential indicates that Fe(III) reduction, methanogenesis, nitrate and sulphate reduction are the main factors controlling biodegradation.  相似文献   

Prevalence of Babesia canis, Borrelia afzelii, and Anaplasma phagocytophilum infection in hard ticks removed from dogs in Warsaw (central Poland)     

Zygner W  Jaros S  Wedrychowicz H 《Veterinary parasitology》2008,153(1-2):139-142

The purposes of this study were to specify the occurrence and prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in ticks removed from dogs in Warsaw, and to determine the Borrelia species occurring in Ixodes ricinus ticks. Among 590 collected ticks, 209 were identified as I. ricinus, and 381 as Dermacentor reticulatus. DNA of B. canis was detected in 11% of D. reticulatus ticks. We found that 6.2% of I. ricinus ticks harbored B. burgdorferi s.l. specific DNA and 2.9% harbored A. phagocytophilum DNA. In these samples sequencing of the detected Borrelia amplicon confirmed infection with Borrelia afzelii genospecies. New sequences were submitted to the GenBank database (accession no. EU152128, EU152127, EU152126). This work is the first detection of B. afzelii and A. phagocytophilum in ticks from Warsaw, and the first survey for the prevalence of B. canis, B. afzelii, and A. phagocytophilum in ticks in central Poland.  相似文献   

Agreement between ELISA and complement fixation test used for diagnosing of paratuberculosis in goats     

Kaba J  Gerlach GF  Nowicki M  Rypuła K 《Polish journal of veterinary sciences》2008,11(3):209-212

An ELISA with a lipoarabinomannan as an antigen, developed for diagnosis of bovine paratuberculosis, has been adapted for use in goats, and compared with complement fixation test. Kappa value of 0.62 indicated good agreement between CFT and the adapted ELISA and proved that the investigated ELISA may be helpful in diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats. The ELISA has been used to screen a randomly selected representative sample of Polish breeding goat population (21.78% of herds, 21.33% of goats). It has been demonstrated that only 2.42% of animals coming from 15.79% of herds were seropositive. Within-herd seroprevalence varied from 1.69% to 38.10%. Most of the infected animals (67.07%) were 3- 4-years-old. No seropositive cases were found in group up to 1-year-old animals.  相似文献   

Xenoestrogens: mechanisms of action and some detection studies     

Słomczyńska M 《Polish journal of veterinary sciences》2008,11(3):263-269

  相似文献   

The effect of copper nanoparticles and copper (II) salt on redox reactions and epigenetic changes in a rat model     

Katarzyna Ognik  Ewelina Cholewi&#x;ska  Jerzy Jukiewicz  Zenon Zdu&#x;czyk  Krzysztof Tutaj  Rados&#x;aw Szl&#x;zak 《Journal of animal physiology and animal nutrition》2019,103(2):675-686

The aim of the study was to evaluate the effects of a diet containing different levels of Cu in two different chemical forms (carbonate and nanoparticles) on redox reactions and epigenetic changes in a rat model. For 4 weeks, five experimental groups (eight rats in each) were fed diets with two dosages of added Cu (standard—6.5 mg/kg or half of the standard dosage—3.25 mg/kg, and as a negative control no additional Cu in the mineral mixture) in two forms (standard—CuCO₃ and copper nanoparticles). Addition of Cu nanoparticles resulted in higher Cp (ceruloplasmin) activity and LOOH (lipid peroxides) and MDA (malondialdehyde) content, as well as decrease the CAT (catalase) activity and level of PC (protein carbonyl), 3‐NT (3‐nitrotyrosine), 8‐OHdG (8‐hydroxydeoxyguanosine), GSH + GSSG (total glutathione) and DNA methylation. Reducing the dose of copper resulted in a decrease in the level of LOOH and GSH + GSSG as well as CAT activity, but increased the level of PC and methylated DNA. Based on these evidence, we concluded that addition of copper nanoparticles in the diet reduces protein oxidation and nitration as well as DNA oxidation and methylation. Lowering the level of Cu in the diet increases the oxidation of proteins and DNA methylation.  相似文献   

Decreases in urine specific gravity and urinary creatinine in dogs with different diseases but with normal renal function     

Fojut-Pałka B  Winnicka A 《Polish journal of veterinary sciences》2008,11(1):41-46

The subject of this study was to determine urine specific gravity (USg) and urinary creatinine (UCrn) in dogs with different diseases but with normal renal function. Sick dogs with different diseases were divided into nine groups. Dogs suffering from polyuria/polydipsia, vomits, diarrhoea and females in oestrus or pregnant were excluded from the studies. The healthy dogs served as a control group. Over a three-year period, a total of 267 dogs were examined clinically as well as using imaging and laboratory diagnostics methods. In sick dogs, USg and UCrn were found to be essentially decreased (except animals with neurological and uterine diseases, and neurological diseases, respectively), as compared with normal dogs. In clinically healthy animals of the control group, UCrn and USg did not significantly differ between the females and males. As for the control group, no correlation between UCrn/USg and the body weight/age was found, either.  相似文献