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排序方式： 共有139条查询结果，搜索用时 15 毫秒
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Asian grapevine leaf rust (AGLR) causes severe crop losses in Brazilian viticulture, mainly in latitudes <25°S. The purpose of this study was to identify the pathogen(s) involved with AGLR in Brazil, based on phylogenetic and morphological analysis and pathogenicity tests. In total, 56 monouredinial isolates from six Brazilian states were identified using the internal transcribed spacer 2 and the large subunit rRNA gene D1/D2 regions. All 50 isolates from the south-central region were classified as Neophysopella tropicalis, and the other six isolates from the north-east region as Neophysopella meliosmae-myrianthae. This result provides evidence that two pathogen introductions from different sources may have occurred in the country. For both species, paraphyses were cylindrical, incurved, aseptate, and hyaline, while urediniospores were short-pedicellate, obovoid or obovoid-ellipsoid, with the wall colourless or pale yellowish, evenly echinulate. Representative isolates from both species caused typical AGLR symptoms on Vitis vinifera 'Merlot' and V. labrusca 'Niagara Rosada'. Overall, regardless of the Neophysopella species, isolates caused similar leaf disease severities. Higher disease severity was observed in Niagara Rosada (average of 40.3% of diseased leaf area) compared to Merlot (20.5%). This study reports, for the first time, the characterization of Neophysopella species associated with AGLR in Brazil. 相似文献
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Isabela V. Primiano Mariana C. Cia Luis E. A. Camargo Lilian Amorim 《Plant pathology》2021,70(2):358-366
Neophysopella tropicalis, one of the causal agents of Asian grapevine leaf rust (AGLR), can cause severe epidemics in Brazil that lead to yield losses in commercial vineyards. An early detection of the pathogen by air sampling of urediniospores on spore traps or in symptomless leaves would be valuable to multiple studies, such as epidemics modelling, risk forecasting, monitoring of pathogen introductions in rust-free areas, and predicting the beginning of epidemics. This study developed a quantitative PCR (qPCR) protocol to quantify N. tropicalis urediniospores attached to adhesive tapes and in grapevine leaves before symptom appearance. A specific primer pair was designed based on the internal transcribed spacer (ITS) sequence region of the AGLR pathogen. Standard amplification curves using genomic DNA from urediniospores of N. tropicalis and from urediniospores attached to adhesive tapes were established. Grapevine leaves inoculated with N. tropicalis were collected at 2, 5, and 7 days postinoculation (dpi). One primer pair (580F/720R) amplified a 140 bp product in all AGLR isolates but did not amplify products of other rust genera, such as Phakopsora, Puccinia, Hemileia, Tranzschelia, Cerotelium, and Coleosporium. As little as 0.1 pg DNA and 10 urediniospores of N. tropicalis attached to adhesive tapes could be detected. qPCR enabled the detection of the pathogen as early as 2 dpi, before symptom appearance. This method can be used to monitor N. tropicalis inoculum in grapevine-growing areas and to quantify symptomless infections of the AGLR pathogen. 相似文献
25.
甘蔗属及近缘属8种植物叶片下表皮的解剖研究 总被引：2，自引：0，他引：2
观察了云南省 8种甘蔗属及近缘属植物叶片下表皮的解剖结构 ,结果表明 ,肋区中刺细胞的有无、行数及形状 ,硅细胞及栓细胞的排列方式 ,表皮上附属物的类型 ,气孔的行数及副卫细胞的形状等在不同种之间是不同的 ,这些解剖特征可作为外部器官形态分类的补充 ,也可为甘蔗杂交育种提供解剖学依据 相似文献
26.
Diego Corrêa Alves Lilian Paula Vasconcelos Robson Marcelo Rossi Sidnei E. Lima-Junior Yzel R. Súarez 《Ecology of Freshwater Fish》2020,29(4):574-587
Bayesian inference was used to estimate the main population parameters (sex ratio, L50, LWR and somatic growth) and to evaluate sexual dimorphism and growth patterns (isometric/allometric and monophasic/biphasic growth) of Astyanax paranae of the Upper Paraná River. This study brings three statistical differentials: (a) the reparametrisation of the logistic model as a function of L50; (b) the reparameterisations of the LWR models based on the exponential of a piecewise linear regression to evaluate the hypotheses of polyphase growth; and (c) the first application of the recently proposed somatic growth models based only on length data to real data, as well as its expansion and generalisation. The models estimated L50 of females at 37.75 mm and sex ratio at 1.4 females per male. LWR models showed that both sexes presented biphasic growth with a lower increase in weight for males in first stanza. In second stanza, both sexes presented a greater increase in weight. Growth models showed that the growth rates were low, equal for both sexes in early life and slightly higher for females after the eighteenth months old. The studied population inhabits streams, therefore being exposed to constraining conditions that can limit the expression of their whole growth potential since they prioritise energy allocation for reproduction and survival, including reducing L50 in order to ensure the maintenance of the population. Finally, the complex biological patterns that arose from the proposed LWR and growth models demonstrated high potential and applicability for population studies. 相似文献
27.
芥菜型油菜(Brassica Juncea)杂种优势利用的研究 总被引：3，自引：0，他引：3
1973年,本院发现芥菜型油菜雄性不育株后,经连续几年研究,1975年育成了欧新 A 不育系,欧新 B 保持系和74243—6恢复系,实现了芥菜型“三系”配套。多年来连续测定表明,育成的芥菜型“三系”表现育性稳定,欧新 A 不育系的不育株率达100%,单株自交结实指数平均在0.000—0.103之间。恢复株率为99.54～100%,F_1杂种有较强的苗期优 相似文献
28.
BACKGROUND: Natural pesticides based on plant essential oils may represent alternative crop protectants. This study analysed the chemical constituents and bioactivities of essential oils from Schinus areira L., Rosmarinus officinalis L. and Tagetes terniflora Kunth against winged and wingless adults of Metopolophium dirhodum (Walk.). RESULTS: The major component of S. areira was α‐phellandrene, that of R. officinalis was 1,8‐cineole and that of T. terniflora was cis‐ocimene. Rosemary essential oil was more effective than the others in immersion method tests. In contact toxicity, the rosemary oil was the most toxic to wingless adults and the S. areira leaf oil was the most toxic to winged adults. All the EOs produced some degrees of repellency in adults and effects on the reproduction and demographic parameters. CONCLUSION: These results showed that the essential oils from S. areira, R. officinalis and T. terniflora could be used as an alternative in the management of M. dirhodum. Copyright © 2012 Society of Chemical Industry 相似文献
29.
Tiago Osório Ferreira Xosé L. Otero Valdomiro S. de Souza Junior Pablo Vidal-Torrado Felipe Macías Lilian P. Firme 《Journal of Soils and Sediments》2010,10(6):995-1006
Purpose
Among environmental factors governing innumerous processes that are active in estuarine environments, those of edaphic character have received special attention in recent studies. With the objectives of determining the spatial patterns of soil attributes and components across different mangrove forest landscapes and obtaining additional information on the cause–effect relationships between these variables and position within the estuary, we analyzed several soil attributes in 31 mangrove soil profiles from the state of São Paulo (Guarujá, Brazil).Materials and methods
Soil samples were collected at low tide along two transects within the Crumahú mangrove forest. Samples were analyzed to determine pH, Eh, salinity, and the percentages of sand, silt, clay, total organic carbon (TOC), and total S. Mineralogy of the clay fraction (<2 mm) was also studied by X-ray diffraction analysis, and partitioning of solid-phase Fe was performed by sequential extraction.Results and discussion
The results obtained indicate important differences in soil composition at different depths and landscape positions, causing variations in physicochemical parameters, clay mineralogy, TOC contents, and iron geochemistry. The results also indicate that physicochemical conditions may vary in terms of different local microtopographies. Soil salinity was determined by relative position in relation to flood tide and transition areas with highlands. The proportions of TOC and total S are conditioned by the sedimentation of organic matter derived from vegetation and by the prevailing redox conditions, which clearly favored intense sulfate reduction in the soils (～80% of the total Fe is Fe-pyrite). Particle-size distribution is conditioned by erosive/deposition processes (present and past) and probably by the positioning of ancient and reworked sandy ridges. The existing physicochemical conditions appear to contribute to the synthesis (smectite) and transformation (kaolinite) of clay minerals.Conclusions
The results demonstrate that the position of soils in the estuary greatly affects soil attributes. Differences occur even at small scales (meters), indicating that both edaphic (soil classification, soil mineralogy, and soil genesis) and environmental (contamination and carbon stock) studies should take such variability into account.30.
João Paulo Rodrigues Marques Marcel Bellato Spósito Alexandre Furtado Silveira Mello Lilian Amorim Matheus Mondin Beatriz Appezzato-da-Glória 《European journal of plant pathology / European Foundation for Plant Pathology》2012,133(2):439-448
In Brazil, citrus black spot (CBS) caused by Guignardia citricarpa is a major disease that has different symptoms on fruit. In this study, fruit of Citrus sinensis infected by G. citricarpa and showing the symptoms false melanosis, freckle spot and hard spot were cross-sectioned and analysed anatomically and histochemically
by light microscopy. Immuno-histological assays were performed. All symptoms were accompanied by a thickening of the cuticle.
False melanosis lesions did not contain pycnidia and remained restricted to the epicarp or to the first layers of the mesocarp.
The stomata in this type of lesion showed phenolic compounds in the guard cells and in the sub-stomatal chamber. In some samples,
the guard cells and their surrounding cells lysed, and a wound meristem began to form underneath them. Freckle spot and hard
spot lesions had very similar histological alterations to the epicarp and mesocarp, but in our samples only hard spot lesions
contained pycnidia. Both of these symptoms were accompanied by protein inclusions. Epidermal and sub-epidermal cells located
in the oil-gland region were obliterated, causing alterations in these structures. All symptoms had regions that stained strongly
for lipids and phenols. 相似文献