青枯菌潜伏浸染对花生的影响   总被引：2，自引：0，他引：2  

廖伯寿  李栋  单志慧  雷永  谈宇俊  段乃雄  唐桂英 《中国油料作物学报》1997,(4)

用青枯菌对20个花生品种进行人工接种，研究了青枯菌潜伏侵染对花生根系、根瘤数、生物产量及结实性状的影响。结果表明，青枯菌潜伏侵染对花生生长发育有普遍的抑制作用，不同花生品种对潜伏浸染的反应存在广泛差异。  相似文献   

三系杂交稻新组合鄂籼杂1号的选育与应用     

胡旭  胡培中  段洪波  王明涛  涂志杰  张宇飞  徐国华  舒冰  袁广平 《杂交水稻》1997,(6)

鄂籼杂１号系用新恢复系０９２－８－８与珍汕９７Ａ配组育成的三系杂交水稻新组合。具有高产、稳产、米质好、耐肥耐寒、抗倒伏、再生能力强、适应性广等优点。１９９３～１９９６年在湖北省已累计示范种植７．４万ｈｍ２，平均单产７．５ｔ／ｈｍ２以上。  相似文献   

高产抗稻瘟病杂交中籼新组合Ⅱ优80   总被引：2，自引：0，他引：2  

杨隆维  袁利群  王光建  李继辉  段太品  许敏  向极钎  高剑华  姚昌顺  彭耀炳 《杂交水稻》2006,21(1):75-76

Ⅱ优80是湖北省恩施州农科院用不育系Ⅱ-32A与自育恢复系恩恢80配组选育而成的杂交中籼新组合,具有对稻瘟病抗性强、耐寒、丰产稳产性好、适应性广和制种易获高产等特点,2005年3月通过湖北省品种审定。介绍了Ⅱ优80的栽培和制种技术要点。  相似文献   

黄淮中南部夏大豆新品种选育技术指标量化分析     

段国占  薛培明  任建军  马向利  陈伟伟 《大豆科技》2007,(5):35-37

通过对河南省21年来审定大豆品种的主要特征特性分析,总结出黄淮夏大豆增产的主要原因是分枝数、株荚数的增加;黄淮夏大豆新品种选育的主要指标是有限结荚习性,有效分枝在3～4个,株荚数在50个以上,百粒重在15.0～24.0 g之间,高蛋白、高油或蛋脂双高.  相似文献   

橡胶树胶乳cDNA文库的构建及其EST序列的功能分析     

段翠芳  曾日中  聂智毅  黎瑜  魏小弟 《热带作物学报》2007,28(3):46-51

  相似文献   

Insulin-like growth factor I (IGF-I) mRNA expression in coho salmon,Oncorhynchus kisutch: Tissue distribution and effects of growth hormone/prolactin family proteins     

Cumming Duan  Stephen J. Duguay  Erika M. Plisetskaya 《Fish physiology and biochemistry》1993,11(1-6):371-379

To examine the hormonal and nutritional regulation of insulin-like growth factor I (IGF-I) mRNA expression, a sequence-specific solution hybridization/RNase protection assay for coho salmon IGF-I mRNA was developed. This assay is both rapid and sensitive and has low inter- (less than 15%) and intra-assay variations (less than 5%). Using this assay, the tissue distribution of IGF-I mRNA and effects of growth hormone (GH), prolactin (PRL) and somatolactin (SL) on hepatic IGF-I mRNA expression in coho salmon were examined in vivo. Liver had the highest IGF-I mRNA level of 16 pg/μg DNA. Significant amounts of IGF-I mRNA were also found in all other tissues examined (intestine 4.1, kidney 3.8, gill arch 2.4, brain 2.4, ovary 2.3, muscle 2.1, spleen 1.7 and fat 1.1 pg/μg DNA). Injection of coho salmon GH at doses of 0.1 and 1 μg/g body weight significantly increased the hepatic IGF-I mRNA levels in a dose-dependent manner. Injection of coho salmon SL, a recently discovered member of the GH/PRL family, stimulated the IGF-I mRNA expression at the higher dose (1 μg/g), whereas coho salmon PRL had no effect at either dose. Concentration-dependent stimulation by coho salmon GH was also obtained in vitro in primary culture of salmon hepatocytes in concentrations ranging from 0.01 to 1 μg/ml. These results indicate that IGF-I mRNA expression occurs in a variety of tissues in coho salmon, and that at least the hepatic expression is under the regulation of GH and possibly other hormones. The sequence-specific assay established in the present study can be used for accurate quantitation of IGF-I mRNA in salmonid species, and can contribute to a better understanding of the physiology of IGF-I in salmonids.

---

Résumé Afin d'étudier les régulations homronales et nutritionnelles de l'expression des ARNm de l'IGF-I (insulin-like growth factor I), un dosage spécifique par hybridation en solution des ARNm d'IGF-I de saumon coho et protégé des RNases, a été développé. Ce dosage, à la fois rapide et sensible, présente un faible coefficient de variation inter- (< 15%) et intra- (< 5%) dosage. L'étude de la distribution tissulaire des ARNm de l'IGF-I et des effets de l'hormone de croissance (GH), de la prolactine (Prl) et de la somatolactine (SI) sur l'expression hépatique des ARNm de l'IGF-I, a été entreprise in vivo chez le saumon coho en utilisant ce dosage. Le foie présente les plus grandes quantités d'ARNm d'IGF-I (16 pg/μg d'ADN). Des quantités significatives d'ARNm d'IGF-I ont été également détectées dans tous les autres tissus étudiés (intestin 4,1; rein 3,8; branchie 2,4; ovaire 2,3; muscle 2,1; rate 1,7 et graisse 1,1 pg/μg d'ADN). L'injection à des saumons coho, de GH à des doses de 0,1 et 1 μg/g de poids vif, augmente significativement et de manière dose dépendante les niveaux hépatiques d'ARNm d'IGF-I. L'injection de SI de saumon coho, un membre récemment découvert de la famille GH/Prl, stimule avec la plus haute dose utilisée, l'expression des ARNm d'IGF-I alors que la Prl n'a aucun effet. La GH augmente de manière dose dépendante (0,01–1 μg/ml) l'expression in vitro des ARNm d'IGF-I par des ARNm d'IGF-I par des hépatocytes de saumon coho en culture. Ces résultats indiquent que, chez le saumon coho, l'expression des ARNm d'IGF-I est présente dans le nombreaux tissus et que, l'expression hépatique est, au moins en partie, régulée par la GH et peut-être par d'autres hormones. Le dosage par séquence spécifique mise au point dans le présent travail, peut-être utilisé pour la quantification précise des ARNm, d'IGF-I de salmonidés et devrait permettre une meilleure connaissance de la physiologie de L'IGF-I chez les salmonidés.

  相似文献   

Determination of ligustilide in the brains of freely moving rats using microdialysis coupled with ultra performance liquid chromatography/mass spectrometry     

Guo J  Shang EX  Duan JA  Tang Y  Qian D 《Fitoterapia》2011,82(3):441-445

In this paper, microdialysis combined with ultra performance liquid chromatography/mass spectrometry (UPLC-MS) was applied for the determination of ligustilide in freely moving rats' brain. The extracellular unbound ligustilide could be detected in rat brain within the first time interval (0-20 min) after nasal administration but not after oral administration. This result showed that ligustilide could quickly enter the brain after nasal administration; which implied that ligustilide may have a rapid onset of action. This work demonstrated the potential application of microdialysis combined with UPLC-MS in pharmacokinetic studies. Furthermore, the advantage that fewer animals used in the microdialysis experiment was confirmed.  相似文献   

Links between plant diversity, carbon stocks and environmental factors along a successional gradient in a subalpine coniferous forest in Southwest China     

Yuanbin ZhangBaoli Duan  JunRen XianHelena Korpelainen  Chunyang Li 《Forest Ecology and Management》2011,262(3):361-369

In all, 48 sites of subalpine coniferous forest that had undergone natural regeneration for 5-310 years were selected as study locations in the Southwest China. We compared species richness (S), plant diversity (Shannon-Wiener index, H′; Margalef index, R), and above- and below-ground ecosystem carbon (C) pools of six plant communities along a chronosequence of vegetation restoration, and we also examined evidence for a functional relationship between plant diversity and C storage. Our results showed that above-ground C increased significantly (over 52-fold), mainly due to the increase of C in aboveground living plants and surface litter. Soil organic carbon (SOC) content increased from the herb community type (dominated by Deyeuxia scabrescens, P1) to mixed forest type (dominated by Betula spp. and Abies faxoniana, P4), which constituted the main C pool of the system (63-89%), but decreased thereafter (communities P5-P6). The mean C stock in the whole ecosystem - trees, litter layer and mineral soil - ranged from 105 to 730 Mg C ha⁻¹ and was especially high in the spruce forest community type (dominated by Picea purpurea, P6). On the other hand, the relationships between C stocks (soil, aboveground) and mean annual temperature or altitude were generally weak (P > 0.05). Moreover, we did not detect a relationship between S and aboveground C storage, while we found a significant negative relationship between H′, R and aboveground C storage. In addition, our experiment demonstrated that total root biomass and litter C/N ratio were significant functional traits influencing SOC, while S, R, and H′ had little effect. Path analysis also revealed that litter C/N ratio predominantly regulated SOC through changes in the quantity of microorganisms and soil invertase enzyme activity.  相似文献   

四川雅安地区2种大型丛生竹耐低温能力   总被引：1，自引：0，他引：1  

胡尚连  卢学琴  陈其兵  曹颖  邓小波  段宁  王自勇 《竹子研究汇刊》2011,30(2)

以慈竹和撑绿杂交竹为材料,对四川雅安地区不同海拔2竹种叶片进行净光合速率测定,并对采取的竹叶的POD活性、可溶性蛋白和可溶性糖含量、MDA含量、脯氨酸含量以及光合色素进行测定和分析,目的在于探讨慈竹和撑绿杂交竹耐低温的生理机制.结果表明,海拔对慈竹和撑绿杂交竹的POD酶活性、可溶性蛋白含量、脯氨酸含量和可溶性糖含量的影响较大,对MDA含量影响不大.低海拔时,撑绿杂交竹具有高的可溶性蛋白含量和脯氨酸含量,慈竹的POD酶活性和可溶性糖含量很高.高海拔时,慈竹的POD酶活性、可溶性蛋白含量、脯氨酸含量和可溶性糖含量都比撑绿杂交竹高,且差异都达到极显著水平,这可能是慈竹净光合速率极显著高于撑绿杂交竹的一个原因,其中可溶性蛋白含量和脯氨酸含量对慈竹具有高净光合速率可能起到很大调节作用.低海拔慈竹和撑绿杂交竹的净光合速率无明显差异,而高海拔慈竹的净光合速率却远高于撑绿杂交竹.慈竹在高海拔的适应性比撑绿杂交竹的强,撑绿杂交竹不适宜在高海拔栽培.  相似文献   

牛大力的生物学特性及其栽培管理     

段左俊  曾祥全  陈飞飞  李新能  刘斌  林声洪 《热带林业》2011,39(4):22-25

在种源收集的基础上,通过对牛大力的根、茎、叶、花、果等形态特征的观察,以及引种地区的环境因素和牛大力生长参数的记录和分析,概述了牛大力的生物学特性和栽培管护措施.  相似文献