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41.
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High-quality standards in blueberry juice can be obtained only taking into account fruit compositional variability and its preservation along the processing chain. In this work, five highbush blueberry cultivars from the same environmental growing conditions were individually processed into juice after an initial blanching step and the influence was studied of the cultivar on juice phenolic content, distribution and relative antioxidant activity, measured as scavenging capacity on the artificial free-radical 2,2-diphenyl-1-picrylhydrazyl (DPPH*). A chromatographic protocol was developed to separate all main phenolic compounds in berries. A total of 15 glycosylated anthocyanins, catechin, galactoside, glucoside, and rhamnoside quercetin 3-derivatives, and main benzoic and cinnamic acids were identified. The total content and relative distribution in anthocyanins, chlorogenic acid, and quercetin of each juice were dependent upon cultivar, and the total content was highly correlated (rxy=0.97) to the antioxidant capacity. A selective protective effect of berry blanching in juice processing can be observed on more labile anthocyanin compounds.  相似文献   
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Experiments were carried out to establish an infection and disease model for Clostridium perfringens in broiler chickens. Previous experiments had failed to induce disease and only a transient colonization with challenge strains had been obtained. In the present study, two series of experiments were conducted, each involving four groups of chickens with each group kept in separate isolators. A coccidial vaccine given at 10 times the prescribed dosage was used to promote the development of necrotic enteritis. In the first experiment, cultures of C. perfringens were mixed with the feed at day 9, 10, 11, and 12, and the coccidial vaccine was given at day 10, whereas in the second experiment, C. perfringens cultures were mixed with the feed at day 17, 18, 19, and 20, and the coccidial vaccine was given at day 18. Chickens were examined at day 9, 11, 12, and 15 (Experiment 1), and at day 17, 18, 20, and 24 (Experiment 2). There was no mortality in any of the groups; however, chickens in the groups receiving both coccidial vaccine and C. peifringens developed the subclinical form of necrotic enteritis, demonstrated by focal necroses in the small intestine, whereas chickens in control groups or groups receiving only coccidial vaccine or only C. perfringens cultures developed no necroses. The results underline the importance of predisposing factors in the development of necrotic enteritis.  相似文献   
45.
DDT 是防治白翅叶蝉的良好药剂。25?T 乳剂1∶200和1∶300倍液的毒效均在90%以上;1∶400倍液的毒效为75—95%。2.5—5?T 粉剂的毒效也达90%以上。田间使用 DDT 防治白翅叶蝉的残效期约为10—12天。666单独用来防治白翅叶蝉效果甚差,但与 DDT 混合使用有增效作用,残效期与单独使用 DDT 相似;二者混用是最合理的施用方式,可以节省 DDT 用药量,减低药费,同时对多种稻虫可收综合防治之效。25?T 乳剂和6%可湿性666按1∶1∶400及1∶1∶600混用,其毒效相当于单用 DDT 1∶200及1∶300者;用1∶1∶500喷射效果比单用 DDT 1∶300者还略高;5% DDT 粉剂和0.5f6粉剂按1∶1混用,其毒效也和单用5% DDT 粉剂相接近。  相似文献   
46.
关中棉花枯萎病及其防治研究   总被引:3,自引:1,他引:2  
棉花枯萎病在陕西关中,近几年由局部地区发展到点面发生,蔓延速度和面积已超过黄萎病。一般发病和死苗在30—50%,严重的棉田在90%以上。一些黄萎病区逐渐转为枯、黄萎混合发生区,枯黄萎同株型极为普遍。此病在棉花苗期即表现症状,潛育期为10—20天。1—2片真叶发病率达10—15%;5—6片真叶(蕾期)即会引起大量枯死和萎蔫。土壤致病温度平均在20°—25℃,维持10天以上,发病最烈;28℃以上病势减轻。土壤含水量在60—75%之间,枯萎病发病率最高。每年枯萎病发生消长与5、6月份的温、湿度及降雨量有一定关系。以4—6片真叶期,枯死植株发展较快,开花后期枯死率显著下降,到吐絮期尚有高峯出现。各品种间的枯死规律基本相同;仅有枯死程度上的差异。防治棉花枯萎病主要途径以抗病品种为主。由1956—1961年已鉴定出抗病品种(系)有52-128、鸭棚、147-Φ、57-50、南通二号、F96,F80等。其中确定能在病区生产应用的有52-128,鸭棚,平均降低发病率在30—70%,增产15—40%左右。并发现抗病免疫类型有中棉“嘉定硬子”。远缘杂交材料(353×G 1472)×芙蓉葵353 F_2可作杂交原始亲本材料。在病区增施氮、磷无机肥料,有降低苗期枯萎病的作用。轮作防病在关中采取二年小(?)、玉米轮作倒茬,发病率由原来的85%降低到38%,死苗率由50%下降到20%,有一定防病增产效果。  相似文献   
47.
OBJECTIVE: To compare the structural properties of interlocking nails (ILNs) locked with bolts (ILNb) to ILN locked with extended bolts connected with a type-IA external skeletal fixator (ILN-ESF) in a fracture gap model. STUDY DESIGN: Experimental study. SAMPLE POPULATION: Synthetic tibial bone substitutes. METHODS: Custom-made synthetic tibial bone substitutes were implanted with standard ILNs locked with either bolts or extended bolts connected to an external skeletal fixation (ESF). Constructs were tested in torsion, bending, and axial compression (n=4/testing mode). Data, consisting of construct compliance and associated deformation, were compared using t-tests. RESULTS: The ILN-ESF construct compliance and deformation were significantly less than those of the ILNb construct in torsion, bending, and compression (P<.001). Slack was present in the ILNb construct under torsion and bending, but not in the ILN-ESF construct, regardless of testing mode. CONCLUSIONS: Substitution of locking bolts with extended bolts connected to an ESF significantly reduced the construct compliance and overall deformation in torsion, bending, and compression. Furthermore, the inherent slack of the ILNb was eliminated by the use of an ESF in torsion and bending. CLINICAL RELEVANCE: The improvement in structural properties of the ILN-ESF constructs could diminish interfragmentary motion at the fracture site and potentially improve bone healing.  相似文献   
48.
王宇清 《作物学报》1985,11(3):159-162
致瘤农杆菌〔Agrobacterium tumefaciens(Smith and Townsend)Conn〕的 C_(58)T_(37)、B3/73菌株被用来感染 Beta vulgaris 和 B.maritima 二个种的106个品种和品系。在102个品种上诱导出冠瘿瘤,其中在2个 B.vulgaris 品种和一个野生杂种(B.maritima×B.vulgaris var.Cicla)诱导出畸胎瘤。利用不合激素的 MS 培养基初步分离出  相似文献   
49.
A new continuous cell line (GF-1) was established and characterized. The GF-1 cell line, derived from the fin tissue of a grouper, Epinephelus coioides (Hamilton), was maintained in L15 medium containing 5% foetal bovine serum (FBS) at 28 °C, and has been subcultured more than 160 times since 1995. The majority of GF-1 cells are fibroblast-like, together with some epithelioid cells. Spontaneous transformation of GF-1 cells occurred during subculture 50 to subculture 80, and led to an increase of plating efficiency, less requirement of FBS and de novo susceptibility to grouper nervous necrosis virus (GNNV). Cytopathic effects (CPEs) could be observed in GF-1 cells 3–5 days post-infection with pancreatic necrosis virus (IPNV), hard clam reovirus (HCRV), eel herpes virus Formosa (EHVF) and GNNV. In addition, abundant GNNV particles were found in the cytoplasm of GNNV-infected GF-1 cells using electron microscopy and nucleic acids of GNNV virus were detected by polymerase chain reaction in the culture medium of GNNV-infected cells after CPE appeared. The experimental results indicated that GF-1 can effectively proliferate fish nodavirus and is a promising tool for studying fish nodavirus.  相似文献   
50.
The black‐lipped pearl oyster, Pinctada margaritifera, is the most important farmed species in French Polynesia and the basis of the most valuable export industry. Mass production of black pearls relies on a surgical operation requiring tissue from a donor pearl oyster to be grafted, together with a nucleus made of shell, into the gonad of a recipient oyster. Improving pearl size through family selection remains one of the main challenges for future aquaculture development. This study analyses the relative contribution of donor and recipient oysters to pearl size. To this end, hatchery‐produced donor oysters of two batches, large and small (based on shell height), were used to supply grafts for recipients, which were then monitored individually for their growth performance by recording shell height, width, and thickness, and total live weight (flesh + shells) every 6 months (four biometric measurement times) over 20 months of culture. Pearls issued from the two batches of donors showed no significant differences in nacre weight or thickness. In contrast, recipient oyster shell height and total weight were increasingly positively correlated with these pearl size parameters over the culture period, becoming significant at 8 months post‐grafting. Potential therefore exists to use shell height and oyster weight as phenotypic indicators for selective breeding of recipient oysters with high growth performance to increase pearl size in P. margaritifera.  相似文献   
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