Heparanase and Syndecan-4 Are Involved in Low Molecular Weight Fucoidan-Induced Angiogenesis     

Oualid Haddad  Erwan Guyot  Nicolas Marinval  Fabien Chevalier  Lo?c Maillard  Latifa Gadi  Christelle Laguillier-Morizot  Olivier Oudar  Angela Sutton  Nathalie Charnaux  Hanna Hlawaty 《Marine drugs》2015,13(11):6588-6608

Induction of angiogenesis is a potential treatment for chronic ischemia. Low molecular weight fucoidan (LMWF), the sulfated polysaccharide from brown seaweeds, has been shown to promote revascularization in a rat limb ischemia, increasing angiogenesis in vivo. We investigated the potential role of two heparan sulfate (HS) metabolism enzymes, exostosin-2 (EXT2) and heparanase (HPSE), and of two HS-membrane proteoglycans, syndecan-1 and -4 (SDC-1 and SDC-4), in LMWF induced angiogenesis. Our results showed that LMWF increases human vascular endothelial cell (HUVEC) migration and angiogenesis in vitro. We report that the expression and activity of the HS-degrading HPSE was increased after LMWF treatment. The phenotypic tests of LMWF-treated and EXT2- or HPSE-siRNA-transfected cells indicated that EXT2 or HPSE expression significantly affect the proangiogenic potential of LMWF. In addition, LMWF increased SDC-1, but decreased SDC-4 expressions. The effect of LMWF depends on SDC-4 expression. Silencing EXT2 or HPSE leads to an increased expression of SDC-4, providing the evidence that EXT2 and HPSE regulate the SDC-4 expression. Altogether, these data indicate that EXT2, HPSE, and SDC-4 are involved in the proangiogenic effects of LMWF, suggesting that the HS metabolism changes linked to LMWF-induced angiogenesis offer the opportunity for new therapeutic strategies of ischemic diseases.  相似文献   

Integrated water resources system dynamics modeling and indicators for sustainable rural community     

Yuan-Hua Li  Pei-Yuan Chen  Wei-Hsuan Lo  Ching-Pin Tung 《Paddy and Water Environment》2015,13(1):29-41

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Pathogenicity bioassays of isolates of Beauveria bassiana on Rhynchophorus ferrugineus          下载免费PDF全文

Gabriella Lo Verde  Livio Torta  Vincenzo Mondello  Cesare G Caldarella  Santella Burruano  Virgilio Caleca 《Pest management science》2015,71(2):323-328

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Chitinolytic Bacteria-Assisted Conversion of Squid Pen and Its Effect on Dyes and Pigments Adsorption     

Tzu-Wen Liang  Bo-Chang Lo  San-Lang Wang 《Marine drugs》2015,13(8):4576-4593

The aim of this work was to produce chitosanase by fermenting from squid pen, and recover the fermented squid pen for dye removal by adsorption. One chitosanase induced from squid pen powder (SPP)-containing medium by Bacillus cereus TKU034 was purified in high purification fold (441) and high yield of activity recovery (51%) by ammonium sulfate precipitation and combined column chromatography. The SDS-PAGE results showed its molecular mass to be around 43 kDa. The TKU034 chitosanase used for the chitooligomers preparation was studied. The enzyme products revealed that the chitosanase could degrade chitosan with various degrees of polymerization, ranging from 3 to 9, as well as the chitosanase in an endolytic manner. Besides, the fermented SPP was recovered and displayed a better adsorption rate (up to 99.5%) for the disperse dyes (red, yellow, blue, and black) than the water-soluble food colorants, Allura Red AC (R40) and Tartrazine (Y4). The adsorbed R40 on the unfermented SPP and the fermented SPP was eluted by distilled water and 1 M NaOH to confirm the dye adsorption mechanism. The fermented SPP had a slightly higher adsorption capacity than the unfermented, and elution of the dye from the fermented SPP was easier than from the unfermented. The main dye adsorption mechanism of fermented SPP was physical adsorption, while the adsorption mechanism of unfermented SPP was chemical adsorption.  相似文献   

Role of the serotonin transporter in heart valve development and disease     

L. M. Pavone  A. Spina  V. Mastellone  R. Lo Muto  E. Fiorillo  L. Avallone 《Veterinary research communications》2009,33(Z1):117-119

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Characterization of flavonoids and pectins from bergamot (Citrus bergamia Risso) peel, a major byproduct of essential oil extraction     

Mandalari G  Bennett RN  Bisignano G  Saija A  Dugo G  Lo Curto RB  Faulds CB  Waldron KW 《Journal of agricultural and food chemistry》2006,54(1):197-203

Bergamot peel is an underutilized byproduct of the essential oil and juice-processing industry. As with other Citrus peels, it still contains exploitable components, such as pectins and flavonoids. Commercial glycoside hydrolases, specifically a combination of pectolytic and cellulolytic enzymes, solubilized a high percentage of the material (81.94%). The flavonoid profile of the peel consisted of characteristic Citrus species flavanone rutinosides and neohesperosides derived from naringenin, eriodictyol, and hesperetin. In addition, a number of minor flavanone and flavone glycosides, not found in orange and lemon peels, were identified. The majority of flavonoids were extracted in the two 70% v/v EtOH extractions. Processing this material clearly has economic potential leading to low environmental impact.  相似文献   

Durum Wheat Bread： Old Sicilian Varieties and Improved Ones     

G.  Gallo  M.  Lo Bianco  R.  Bognanni G. Saimbene  A.  Orlando O. Grillo  R.  Saccone G. Venora 《农业科学与技术》2010,(4):10-17

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Quantitative trait loci influencing fruit-related characteristics of tomato grown in high-temperature conditions     

Kuan-Hung Lin  Wei-Lung Yeh  Huei-Mei Chen  Hsiao-Feng Lo 《Euphytica》2010,174(1):119-135

The objective of this study was to identify molecular markers linked to fruit-related traits in the tomato subjected to high temperatures. In total, 160 F₂ plants derived from a cross between a heat-tolerant breeding line, CL5915-93D4-1-0-3 (Solanum esculentum), and a heat-sensitive wild accession, L4422 (S. pimpinellifolium), were grown in a greenhouse. Six traits including fruit number, fruit weight, brix, seed number, fruit setting, and flower number were scored. The distributions of fruit number, fruit set, flower number, and seed number were skewed towards heat susceptibility which is known to be characteristic of L4422. Polymorphic bands were generated by PCR-derived methods of RAPD, ISSR and AFLP Polymorphism, the segregation ratio, and distribution over the genome of the above 3 markers were compared. Ten linkage groups, ranging 20.6–151.6 cM in size, were constructed with 62 informative markers spanning a total of 776.3 cM. Fruit-related quantitative trait loci (QTLs) were non-randomly distributed in the tomato genome. For the 6 traits investigated, 21 QTLs were dispersed on linkage groups 2–5. The genetic effects of the various QTLs were differently exhibited, in our study we have respectively found from 10.5% to 30.2% of the variation explained by the QTL for flower number (FRN4) and brix (BX2). Thirteen QTL-mapped markers were unique to 1 trait, and 4 markers were linked to more than 1 trait. Among them, QTLs linked to the I868-470 marker had effects on fruit weight and brix, and a significant positive correlation between these 2 traits was noted (r = 0.35, P < 0.05). Thus, the I868-470 marker may have the potential for simultaneous selection of high fruit weight and brix. These markers also allowed us to align genome linkage maps across distantly related species and to reveal the co-localization between these QTLs and major genes.  相似文献   

Computed tomography of proximal metatarsal and concurrent third tarsal bone fractures in a Thoroughbred racehorse     

A. E. Poulin Braim  R. J. W. Bell  J. A. Textor  W. Y. Lo  S. M. Puchalski  L. D. Galuppo 《Equine Veterinary Education》2010,22(6):290-295

A 2‐year‐old Thoroughbred filly presented to the William R. Pritchard Veterinary Medical Teaching Hospital at the University of California at Davis for whole body nuclear scintigraphic evaluation after an acute onset of nonweightbearing right hindlimb lameness post race. The use of computed tomography (CT) enabled visualisation of additional pathology that was not initially appreciable using combined imaging modalities of nuclear scintigraphy and digital radiography, which ultimately altered the selected course of treatment. Computed tomography in horses with fractures of the cuboidal bones of the hock can provide valuable additional information regarding fracture configuration and radiographically occult pathology, which may help to guide clinical decisions about treatment.  相似文献   

Willingness of residents to pay and motives for conservation of urban green spaces in the compact city of Hong Kong     

Alex Y. Lo  C.Y. Jim 《Urban Forestry & Urban Greening》2010,9(2):113-120

People attach multiple values to urban green spaces which play varied roles in cities. Properly designed monetary valuation surveys can ascertain their non-market value and underlying motives. This study investigates Hong Kong residents’ recreational use of urban green spaces and assesses the monetary value of these areas. A total of 495 urban residents from different neighbourhoods and socio-economic groups were interviewed. About 70% of the respondents visited urban green spaces at least weekly. Major companions during patronage were family members and then children. Exercises and clean air topped the list of visit purposes. The recreational pattern is associated with the cramped private living condition that pushes people to public open areas which are construed as extension of home space. The valuation question solicited overwhelming support, with over 80% of the respondents willing to pay to recover a possible loss of urban green spaces area by 20%. It yielded a monthly average payment of HK$77.43 (approx. 9.90 USD) per household for five years. Non-instrumental aspects played some role in the respondents’ bidding decision. The findings could assist green space planning and nature conservation, and hinted the need to consider the pluralistic community views and expectations in relevant public policies.  相似文献