A panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection     

Rosivaldo Quirino Bezerra Júnior  Ângela Maria Xavier Eloy  João Ricardo Furtado  Raymundo Rizaldo Pinheiro  Alice Andrioli  Frederico Bruno Moreno  Marina Duarte Pinto Lobo  Ana Cristina O. Monteiro-Moreira  Renato de Azevedo Moreira  Tatiana Maria Farias Pinto  Maria Fátima da Silva Teixeira 《Tropical animal health and production》2018,50(1):43-48

The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. The results of this study show the reaction of the innate immune system against chronic infection of goats by CAE.  相似文献   

Xenotransplantation of goat ovary as an alternative to analyse follicles after vitrification     

Nathalie Jiatsa Donfack  Kele Amaral Alves  Benner Geraldo Alves  Rebeca Magalhes Pedrosa Rocha  Jamily Bezerra Bruno  Carlos H. Lobo  Marcelo Bertolini  Regiane Rodrigues dos Santos  Marilia de Oliveira Taumaturgo  Ramon da Silva Raposo  Jos Ricardo de Figueiredo  Johan Smitz  Ana Paula Ribeiro Rodrigues 《Reproduction in domestic animals》2019,54(2):216-224

The aim of this study was to evaluate the caprine preantral follicles enclosed on vitrified/warmed ovarian cortex grafted to nude BALB/mice during 1 month. The ovarian cortex from goats was fragmented (3 × 3 × 0.5 mm) and divided into four groups: fresh control, vitrified control, fresh transplant and vitrified transplant. Follicular morphology, development and density, fibrosis as well as apoptosis, and tissue revascularization were evaluated. It was also observed a significant decrease in morphologically normal preantral (primordial, transition, primary and secondary) follicles in both vitrified control and vitrified transplant treatments when compared with both fresh control and fresh transplant. However, fresh control and fresh transplant exhibited a similar percentage of developing follicles. Additionally, Vitrified control showed a significant increase in developing follicles in comparison with both fresh control and fresh transplant. Follicular density significantly decreased in all treatments in comparison with fresh control. We observed high fibrosis in both fresh transplant and vitrified transplant. The mRNA expression of caspase 3 was lower in both fresh transplant and vitrified transplant in comparison with vitrified control. In conclusion, xenotransplantation is an excellent strategy to maintain normal preantral follicle morphology after vitrification/warming of goat ovarian tissue. Yet, in order to ensure the survival and development of these follicles, it is essential to improve the revascularization of the graft.  相似文献   

Redox cycling induces spermptosis and necrosis in stallion spermatozoa while the hydroxyl radical (OH•) only induces spermptosis          下载免费PDF全文

P Martín Muñoz  L Anel‐López  JM Ortiz‐Rodríguez  M Álvarez  P de Paz  C Balao da Silva  H Rodríguez Martinez  MC Gil  L Anel  FJ Peña  C Ortega Ferrusola 《Reproduction in domestic animals》2018,53(1):54-67

Oxidative stress is a major factor explaining sperm dysfunction of spermatozoa surviving freezing and thawing and is also considered a major inducer of a special form of apoptosis, visible after thawing, in cryopreserved spermatozoa. To obtain further insights into the link between oxidative stress and the induction of apoptotic changes, stallion spermatozoa were induced to oxidative stress through redox cycling after exposure to 2‐methyl‐1,4‐naphthoquinone (menadione), or hydroxyl radical formation after FeSO₄ exposure. Either exposure induced significant increases (p < 0.05) in two markers of lipid peroxidation: 8‐iso‐PGF_2α and 4‐hydroxynonenal (4‐HNE). While both treatments induced changes indicative of spermptosis (caspase‐3 activation and decreased mitochondrial membrane potential) (p < 0.01), menadione induced sperm necrosis and a dramatic reduction in motility and thiol content in stallion spermatozoa. Thus, we provided evidence that oxidative stress underlies spermptosis, and thiol content is a key factor for stallion sperm function.  相似文献   

Practices and opinions of New Zealand beef cattle farmers towards bovine viral diarrhoea control in relation to real and perceived herd serological status     

MC Gates  CA Evans  J-H Han  C Heuer  JF Weston 《New Zealand veterinary journal》2020,68(2):92-100

ABSTRACT

Aims: To investigate the seroprevalence of infection with bovine viral diarrhoea (BVD) virus among 75 beef herds and seroconversion in cattle during early pregnancy, and to determine the practices and opinions of farmers towards BVD control and their association with real and perceived herd serological status.

Methods: Blood samples were collected before mating in 75 beef herds across New Zealand from 15 unvaccinated heifers that had delivered their first calf that season. Serum samples were tested for BVD antibodies using ELISA individually, and after pooling samples for each farm. Animals that were antibody-negative were retested at either pregnancy diagnosis or weaning. Farmers were asked to complete a detailed survey about herd demographics, BVD testing and vaccination practices, and opinions towards national BVD control.

Results: Based on the pooled serum antibody ELISA results, there were 28/75 (37%) negative herds, 15/75 (20%) suspect herds, and 32/75 (43%) positive herds. Of 1,117 animals sampled 729 (65.3%) tested negative for BVD virus antibodies; when retested, 47/589 (8.0%) animals from 13/55 (24%) herds had seroconverted. Among 71 famers providing survey responses 11 (15%) believed their herd was infected with BVD, 24 (34%) were unsure and 36 (51%) did not think their herd was infected. Only 19/71 (18%) farmers had performed any BVD testing within the past 5 years and 50/70 (71%) had not vaccinated any cattle for BVD. Support for national BVD eradication programme was strong in 51/71 (56%) respondents, but the biggest challenge to BVD control was considered to be famer compliance. Compared to farmers who did not think their herd was infected, more farmers who thought BVD was present in their herds had previously tested for BVD, would consider testing all replacement calves, and would support establishing a national BVD database; fewer would consider purchasing BVD tested or vaccinated cattle only.

Conclusions and clinical relevance: Only 15% of the beef farmers in this study believed their herds were infected with BVD virus and few of them had undertaken BVD screening. Nevertheless many were supportive of implementing a national BVD control programme. It is likely that the lack of farmer awareness around BVD and the failure of farmers to recognise the potential impacts in their herds are hindering progress in controlling the disease in New Zealand. There are opportunities for New Zealand veterinarians to be more proactive in helping beef farmers explore BVD management options.  相似文献   

Prevalence of desexed cats in relation to age in a convenience sample of Western Australian cats     

J Johnson  MC Calver 《Australian veterinary journal》2014,92(6):226-227

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Prevalence and severity of anthelmintic resistance in ovine gastrointestinal nematodes in Australia (2009–2012)     

MC Playford  AN Smith  S Love  RB Besier  P Kluver  JN Bailey 《Australian veterinary journal》2014,92(12):464-471

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Effect of Suppression of FSH with a GnRH Antagonist (Acyline) Before and During Follicle Deviation in the Mare     

CM Checura  MA Beg  EL Gastal  MO Gastal  MC Wiltbank  JJ Parrish  OJ Ginther 《Reproduction in domestic animals》2009,44(3):504-511

A GnRH antagonist (Acyline) was used to study the role of FSH in early development of a follicular wave in 61 mares. In Experiment 1, a single dose of 3 mg per mare, compared with 0 and 1 mg, suppressed both the FSH and follicle responses to exogenous GnRH. In Experiment 2, high concentrations of FSH were induced by two successive ablations of all follicles ≥ 6 mm on days 10 and 13 (day 0 = ovulation). A single treatment with Acyline resulted in significantly greater suppression of plasma concentrations of FSH than a single treatment with charcoal-extracted follicular fluid (source of inhibin) or oestradiol. Suppression of FSH was not significantly different between the group treated with Acyline alone and a group treated with a combination of Acyline, inhibin and oestradiol. In Experiment 3, all follicles were ablated on day 10 to induce an FSH surge and a new follicular wave. Acyline treatment on day 10 resulted in an immediate decrease in FSH, without a significant effect on day of emergence of a new wave or growth of follicles from 7 to 11 mm on days 11–13. Treatment on day 15, a day before expected follicle deviation and after the peak of the wave-stimulating FSH surge, resulted in an immediate decrease in FSH and cessation of follicle growth. Results indicated that growth of follicles for about 2 days after wave emergence was independent of FSH. In contrast, during the decline in the wave-stimulating FSH surge and before follicle deviation, growth of follicles was dependent on FSH.  相似文献   

hCG‐Induced Ovulation in Thoroughbred Mares Does Not Affect Corpus luteum Development and Function During Early Pregnancy     

B Urquieta  MC Durán  I Coloma  VH Parraguez 《Reproduction in domestic animals》2009,44(6):859-864

Our aim was to compare Corpus luteum (CL) development and blood plasma concentration of progesterone ([P₄]) in thoroughbred mares after spontaneous (Control: C) or human chorionic gonadotrophin (hCG)‐induced ovulation. Lactating mares (C = 12; hCG = 21) were daily teased and mated during second oestrus post‐partum. Treated mares received 2500 IU hCG i.v. at first day of behavioural oestrus when dominant follicular size was >35, ≤42 mm and mated 12–24 h after. Control mares in oestrus were mated with dominant follicular size ≥45 mm. Dominant follicle before ovulation, CL and gestational sac were measured by ultrasound and [P₄] by radioimmunoassay (RIA). Blood sampling and ultrasound CL exams were done at days 1, 2, 3, 4, 8, 12, 16, 20, 25, 30, 35, 40, 45, 60 and 90 after ovulation and gestational sac from day 12 after ovulation in pregnant (P) mares; non‐pregnant (NP) were followed until oestrus returned. Data analyses considered four subgroups: hCG‐P, hCG‐NP, C‐P and C‐NP. Preovulatory follicular size was smaller in hCG mares than in C: 39.2 ± 2.7 mm vs 51.0 ± 1.8 mm (p < 0.0001). All hCG mares ovulated 24–48 h after treatment and presented similar oestrus duration as controls. C. luteum size in P mares showed the same pattern of development through days 4–35, presenting erratic differences during initial establishment. Thus, on days 1 and 3, CL was smaller in hCG‐P (p < 0.05); while in hCG‐NP, CL size was greater than in C‐NP on day three (p = 0.03). Corpus luteum size remained stable until day 90 in hCG‐P mares, while in C‐P a transient and apparently not functional increase was detected on days 40 and 45 (p < 0.05) and the decrease from day 60 onwards, made this difference to disappear. No differences were observed in [P₄] pattern between P, or between NP subgroups, respectively. So, hCG‐induced ovulation does not affect CL development, neither [P₄] during early pregnancy. One cycle pregnancy rate tended to be lower in hCG mares while season pregnancy rates were similar to controls.  相似文献   

Prioritizing areas for conservation and vegetation restoration in post-agricultural landscapes: A Biosphere Reserve plan for Bioko, Equatorial Guinea     

Noelia Zafra-Calvo  Rowena Cerro  Jorge M. Lobo  Sahotra Sarkar 《Biological conservation》2010,143(3):787-794

Although the conversion of natural vegetation to agriculture threatens biodiversity, post-agricultural lands may provide an opportunity to preserve biodiversity if they are allowed to regenerate. We develop a framework for incorporating abandoned agricultural fields into the design of a Biosphere Reserve using former cocoa plantations on Bioko, Equatorial Guinea, as a case study. First, we used BIOCLIM to model the potential distribution of 62 ferns, 327 monocotyledons, 749 dicotyledons, seven primates, and 104 birds on Bioko. Next, we quantitatively assessed the representation of these distributions in conservation areas proposed by the Equatoguinean administration (hereafter “EPAs”). In addition, we used an area prioritization algorithm implemented in the ResNet software package to select an initial set of sites to serve as the Biosphere Reserve’s core areas, that is, intact forest in Bioko’s montane regions. Then, to augment the beta-diversity of the Reserve, we used the area prioritization algorithm to prioritize buffer zones in lowland sites including rainforest remnants and abandoned plantations that have partially regenerated to forest. We also compared the representation of biodiversity in the EPAs to its representation in Biosphere Reserves designed with ResNet. The representation of vegetation types and species in Reserves selected by ResNet that occupy 25% of the land on Bioko is equivalent to the representation achieved by the EPAs, which would cover 42% of Bioko. To conclude, we propose a conservation plan for Bioko.  相似文献   

Use of aortic flow indexes derived from transthoracic echocardiography to evaluate response to a fluid challenge in anesthetized dogs     

Guillermo C.V. de Oliveira  Francisco J. Teixeira-Neto  Tábata L. Dalmagro  Angélica Alfonso  Nathalia Celeita-Rodríguez  César P.C. Lobo  Maria L.G. Lourenço 《Veterinary anaesthesia and analgesia》2021,48(2):187-197

ObjectiveTo evaluate the ability of transthoracic echocardiographic aortic flow measurements to discriminate response to a fluid challenge (FC) in healthy anesthetized dogs.Study designProspective experimental study.AnimalsA total of 48 isoflurane-anesthetized dogs (14.2–35.0 kg) undergoing elective surgery.MethodsFluid responsiveness was evaluated before surgery by FC (lactated Ringer’s 10 mL kg^–1 intravenously over 5 minutes). Percentage increases in transpulmonary thermodilution stroke volume (ΔSV_TPTD) >15% from values recorded before FC defined responders to volume expansion. A group of 24 animals were assigned as nonresponders (ΔSV_TPTD ≤15%). When ΔSV_TPTD was >15% after the first FC, additional FC were administered until ΔSV_TPTD was ≤15%. Final fluid responsiveness status was based on the response to the last FC. Percentage increases after FC in aortic flow indexes [velocity time integral (ΔVTI_FC) and maximum acceleration (ΔVmax_FC)] and in mean arterial pressure (ΔMAP_FC) were compared with ΔSV_TPTD.ResultsAfter one FC, 24 animals were responders. For nonresponders, ΔSV_TPTD was ≤15% after one, two and three FCs in eight/24, 15/24 and one/24 animals, respectively. The FC that defined responsiveness increased ΔSV_TPTD by 29 (18–53)% in responders and by 8 (–3 to 15)% in nonresponders [mean (range)]. The area under the receiver operating characteristics curve (AUROC) of ΔVTI_FC (0.901) was larger than the AUROCs of ΔVmax_FC (0.774, p = 0.041) and ΔMAP_FC (0.519, p < 0.0001). ΔMAP_FC did not predict responsiveness (p = 0.826). Best cut-off thresholds for discriminating responders, with respective zones of diagnostic uncertainty (gray zones) were >14.7 (10.8–17.6)% for ΔVTI_FC and >8.6 (–0.3 to 14.7)% for ΔVmax_FC. Animals within the gray zone were 17% (ΔVTI_FC) and 50% (ΔVmax_FC).Conclusions and clinical relevanceChanges in VTI induced by FC can determine responsiveness with reasonable accuracy in dogs and could play an important role in goal-directed fluid therapy.  相似文献