ACE inhibition in goats under fixed‐time artificial insemination protocol increases the pregnancy rate and twin births          下载免费PDF全文

VP Fernandes Neto  MNN Silva  AS Costa  D Kunkel  AS Júnior  LCS Feitosa  MCS Muratori  APR Costa 《Reproduction in domestic animals》2018,53(4):1006-1008

To assess the effect of the angiotensin‐converting enzyme (ACE) inhibition on the efficiency of the fixed‐time artificial insemination (TAI), 69 goats were divided randomly into two groups: enalapril (n = 35) and control (n = 34). In the experiment, all animals underwent the protocol of fixed‐time artificial insemination for 12 days. Enalapril group received enalapril maleate dissolved in saline (Enalapril, Lab Teuto Ltda) subcutaneously at the following doses: 0.2 mg/kg/day in D0‐D2; 0.3 mg/kg/day in D3‐D6 and 0.4 mg/kg/day in D7‐D11. The control group received the corresponding volume of 0.9% saline solution. We performed a single insemination 36 hr after sponge removal using frozen semen from two adult male goats with recognized fertility. The ultrasound pregnancy diagnosis was 30 days after the artificial insemination (AI). There was significant increase in pregnancy rates and twinning as well as a decrease in foetal loss in animals receiving enalapril (p < .01). The use of ACE inhibitors during the TAI protocol was shown to be a promising alternative to increase the efficiency of such reproductive biotechnology.  相似文献   

Metabolic effects of trichlorfon (Masoten®) on the neotropical freshwater fish pacu (Piaractus mesopotamicus)     

Francine P. Venturini  Fernanda D. Moraes  Lucas R. X. Cortella  Priscila A. Rossi  Claudinei Cruz  Gilberto Moraes 《Fish physiology and biochemistry》2015,41(1):299-309

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Evaluation of Low‐protein Alternative Diets for Pond‐raised Hybrid Catfish,Ictalurus punctatus × Ictalurus furcatus          下载免费PDF全文

Menghe H. Li  Edwin H. Robinson  Penelope M. Lucas  Brian G. Bosworth 《Journal of the World Aquaculture Society》2015,46(2):228-234

A study was conducted to evaluate low‐protein traditional or alternative diets for pond‐raised hybrid catfish, Ictalurus punctatus × Ictalurus furcatus. Three 24% protein diets containing decreasing levels of soybean meal (30, 20, and 15%) and increasing levels of cottonseed meal and corn germ meal were compared with a 28% protein control diet. Hybrid catfish fingerlings (mean initial weight = 71 g/fish) were stocked into 20 earthen ponds (0.04 ha) at a density of 14,826 fish/ha with five ponds per dietary treatment. Fish were fed once daily to apparent satiation for a 191‐d growing season. There were no significant differences in total diet fed, net yield, weight gain, feed conversion ratio (FCR), survival, or fillet proximate nutrient composition among dietary treatments (P ≥ 0.10). However, regression analysis showed for fish fed 24% protein diets there was a linear increase in FCR as soybean meal levels decreased (P = 0.06). Compared with fish fed the 28% protein control diet, fish fed 24% protein diets had lower carcass and fillet yield. Results demonstrate a 24% protein alternative diet containing 20% soybean meal may be substituted for 28% protein diets for hybrid catfish during food fish production.  相似文献   

Early resynchronization protocols for goats: Progestogens can be used prior to an early pregnancy diagnosis without affecting corpus luteum functionality     

Isabel Oliveira Cosentino  Mario Felipe Alvarez Balaro  Felipe Seabra Cardoso Leal  Lucas de Figueiredo Cardoso Barbosa  Fernanda Martins Gonçalves  Gabriel Feliciano Felizardo  Marina Monteiro Netto  Felipe Zandonadi Brandão 《Reproduction in domestic animals》2020,55(11):1655-1659

This study aimed to evaluate the exogenous progesterone (P4) effect on the luteal function from Day 16 to Day 21 of the oestrous cycle in inseminated goats with unknown pregnancy status. A total of 54 does passed through a short progestin-based synchronization protocol and, on Day 16 of the following oestrous cycle, 27 does received a new P4 device which was retained until Day 21. Blood samples were collected daily from all does during this period, as well as on Day 24. Pregnancy diagnoses were performed on Day 30. Serum P4 values from 26 animals (G_NPSP: Group of non-pregnant does with second sponge: n = 8; G_NPNSP: Group of non-pregnant does without second sponge: n = 6; G_PSP: Group of pregnant does with second sponge: n = 5; G_PNSP: Group of pregnant does without second sponge: n = 7) were determined by radioimmunoassay commercial kits. No P4 differences were found between groups (G_NPSP: 3.1 ± 2.8; 1.7 ± 1.8; 0.4 ± 1.0; and 0.0 ± 0.0 vs. G_NPNSP: 4.4 ± 1.8; 3.0 ± 2.2; 0.8 ± 0.8; and 0.0 ± 0.0 or G_PSP: 4.2 ± 1.0; 3.4 ± 0.6; 3.3 ± 1.6; 3.2 ± 0.9; 3.6 ± 1.2; 3.5 ± 1.3; 2.7 ± 1.3 vs. G_PNSP: 4.4 ± 1.6; 3.6 ± 1.5; 3.7 ± 1.5; 3.8 ± 1.4; 3.2 ± 1.2; 3.1 ± 1.2; 3.6 ± 1.1; D16, D17, D18, D19, D20, D21, D24, respectively) or for the interaction of group and time. In conclusion, a second progestogen device had no effect on luteolysis or early pregnancy in the following oestrous cycle.  相似文献   

Dietary plasticity in Arctic charr (Salvelinus alpinus) in response to long‐term environmental change     

L. J. Corrigan  I. J. Winfield  A. R. Hoelzel  M. C. Lucas 《Ecology of Freshwater Fish》2011,20(1):5-13

Corrigan LJ, Winfield IJ, Hoelzel AR, Lucas MC. Dietary plasticity in Arctic charr (Salvelinus alpinus) in response to long‐term environmental change.
Ecology of Freshwater Fish 2011: 20: 5–13. © 2010 John Wiley & Sons A/S Abstract –  In the face of widespread environmental change impacts, there is a need to better understand mechanisms promoting flexibility and resilience of ecosystem components to such change to inform strategies for conservation. Glacial relict species are especially vulnerable to such changes. We investigated the behavioural responses of a native, glacial relict species, Arctic charr (Salvelinus alpinus) to long‐term environmental changes. It was hypothesised that changes in feeding behaviour would occur as a key intermediary to reduction in habitat availability (through climate change and eutrophication) or competitive interactions [with introduced roach (Rutilus rutilus)]. Stomach content analysis was used to assess the diet of 199 charr caught from Windermere, United Kingdom, in the months of March, June, September and November 2003–2007. The results were compared to data from 1940 to 1951 prior to the environmental changes and revealed a marked increase in the contribution of benthic invertebrates in the present diet. Stable isotope analysis confirms the results of the stomach analysis, suggesting that the charr have switched their diet from zooplanktivory towards benthivory. We discuss the possibility that habitat modification and roach population expansion in Windermere have contributed to changes in charr diet. Complementary studies suggest that this diet shift is more likely to be a response to the increasing roach population than to habitat modification; however, further study in Windermere would be required to elucidate the exact mechanism. Long‐term data sets such as these provide information that is useful for determining the directivity of ecological change and the capability of species to respond to change.  相似文献   

Sperm subpopulations influence the pregnancy rates in cattle     

Myrian Megumy Tsunokawa Hidalgo  Ana Beatriz Marques de Almeida  Fábio Lucas Zito de Moraes  Rodrigo Yudi Palhaci Marubayashi  Fabiana Ferreira de Souza  Thales Ricardo Rigo Barreiros  Maria Isabel Mello Martins 《Reproduction in domestic animals》2021,56(8):1117-1127

This study aimed to study the characteristics and subpopulations of spermatozoa from bulls with low and high reproductive performance based on pregnancy rates. Based on historical records of pregnancy rate from four farms, 24 bulls were selected. Two groups were established, with low pregnancy rates (n = 12; LOW), including bulls that presented pregnancy rates <52.27% (33.33% to 51.81%); and a group with high pregnancy rates (n = 12; HIGH), with pregnancy rates >52.27% (52.27% to 69.64%), after fixed-time artificial insemination (FTAI). The thawed sperm straws were analysed to sperm kinetics, morphology, plasma membrane integrity and sperm subpopulations. The LOW group exhibited a higher proportion of static cells (p < .05). In contrast, the HIGH group showed greater percentages for membrane integrity and total and progressive motility, and cells with fast and medium velocity (p < .05). In the cluster procedures, four sperm subpopulations were established. The low-fertility bulls presented the highest percentage of subpopulation 2 (41.46%), characterized by slow and progressive spermatozoa. The high-fertility bulls exhibited the highest percentage of subpopulation 3 (37.17%), characterized by fast and nonlinear spermatozoa. Results from this study indicated that bulls with greater percentages of fast and nonlinear spermatozoa seem to have greater fertilization capacity and the subpopulations analysis can be considered a tool to identify ejaculates with high fertility.  相似文献   

Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report     

Lucas T. Rodrigues  Lorenzo GTM Segabinazzi  Sidnei N Oliveira  Mariana Frasson  Frederico O Papa 《Reproduction in domestic animals》2021,56(11):1470-1474

Pregnancy rates after embryo transfer (ET) are disappointing in donkey species. This study aims to report two successful ET of mini-donkey embryos using Brazilian Northeastern jennies as recipients. Eighteen embryo flushes were performed 9 days post-ovulation in two non-pregnant mini-donkeys jennies (11 and 7 cycles per jenny). Eleven embryos (61%, 11/18) were collected and transferred to Brazilian Northeastern jennies 4–6 days post-ovulation by conventional (n = 6) or an alternative (n = 5) technique. The alternative method consisted of inserting a Polansky equine vaginal speculum smeared with lubricant in the vagina of the recipient jenny. The arms of the speculum were extended to allow the visualization of the cervix. Then, using an adapted crafted, elongated, toothed tissue grasping forceps, the external cervical os was held, and the cervix was gently pulled backward, aiming to straight the cervical canal. The ET gun was inserted through the vagina and cervix by visual inspection, and the embryo was released into the uterine lumen. All embryos collected were Grade 1 and classified as Expanded Blastocysts. No jennies become pregnant after conventional ET (0/6), whereas two recipient jennies (40%, 2/5) become pregnant and delivered offspring in the following year after ET using the alternative technique. In conclusion, Brazilian Northeastern jennies can be used as embryo recipients using the alternative method proposed in the present study. However, further investigations are needed to improve the knowledge and results of ET in donkey species.  相似文献   

Supplementation with Ca salts of soybean oil interacts with concentrate level in grazing dairy cows: milk production and milk composition     

Fernanda Lopes Macedo  Jonas de Souza  Fernanda Batistel  Lucas Jado Chagas  Flávio Augusto Portela Santos 《Tropical animal health and production》2016,48(8):1585-1591

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Effect of supplementation of beef cattle with different protein levels and degradation rates during transition from the dry to rainy season     

Rodolfo Maciel Fernandes  Chafic Mustafé de Almeida  Bruna Caldas Carvalho  João Alexandrino Alves Neto  Verônica Aparecida Costa Mota  Flávio Dutra de Resende  Gustavo Rezende Siqueira 《Tropical animal health and production》2016,48(1):95-101

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Herd-level prevalence and risk factors for bovine viral diarrhea virus infection in cattle in the State of Paraíba,Northeastern Brazil     

Leise Gomes Fernandes  Adriana Hellmeister de Campos Nogueira  Eliana De Stefano  Edviges Maristela Pituco  Cláudia Pestana Ribeiro  Clebert José Alves  Tainara Sombra Oliveira  Inácio José Clementino  Sérgio Santos de Azevedo 《Tropical animal health and production》2016,48(1):157-165

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