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991.
Coupling time to silking with plant growth rate in maize   总被引:1,自引:0,他引:1  
In maize (Zea mays L.), progress towards pistillate flower maturity (silking) is highly dependent upon the environmental conditions around flowering. Under conditions that inhibit plant growth, female flower development is delayed relative to that of the male flowers resulting in an increase in the anthesis–silking interval (ASI). Although variation in ASI has been extensively documented, its relationship to plant growth is not well understood. Therefore, we developed a conceptual basis and experimental approach for quantifying and analyzing the process of female flowering in maize in response to variation in plant growth rate during the flowering period.  相似文献   
992.
Pregnant sows were inoculated with inactivated transmissible gastroenteritis virus and with preparations of virus surface projections and subviral particles derived by detergent treatment of the virus. Neutralising antibody was demonstrated in serum and colostrum from animals that received whole virus or preparations of surface projections whereas subviral particles failed to stimulate neutralising antibody formation. Similar results were obtained with serum from rabbits inoculated with whole virus and structural components. All three preparations stimulated the formation of agglutinating antibodies, as demonstrated by sedimentation analysis and filtration studies with radiolabelled virus.The immunoglobulin classes responsible for neutralising antibody activity in sows inoculated by the intramammary route were examined. In each case where the immunoglobulin class was determined, IgG was found. One sow that received surface projections also had IgA with neutralising activity in her colostrum. In contrast, infection of sows with live whole virus resulted in neutralising antibody of the IgG, IgM and IgA classes.  相似文献   
993.
994.
Three groups of spring-born ram lambs were used to assess breed group differences in testicular growth patterns through 5 mo of age. Group 1 included 20 3/4-Finnish Landrace (Finn), 1/4-Rambouillet rams and 31 1/8-Finn, 7/8-Rambouillet rams. Group 2 included 23 3/4-Finn, 1/4-Dorset rams and 19 1/8-Finn, 7/8-Dorset rams. Group 3 included 21 black-faced (BF) rams (six Hampshire and 15 Suffolk) and 46 whitefaced (WF) rams (nine Dorset, 20 Barbados Blackbelly X Dorset, 10 Finn X Dorset and six Dorset X Finn). Scrotal circumference (C) was measured at 19, 43, 72, 100, 128 and 153 d in Group 1; at 20, 48, 76, 93 and 153 d in Group 2 and at 40, 60, 81, 103, 124 and 158 d in Group 3. Breed group X age interaction was tested to assess breed group differences in testicular growth patterns, and logistic curves were used to describe increases in scrotal circumference with age. In Group 1, 3/4-Finn and 1/8-Finn rams were similar in estimated final scrotal circumference at 150 d of age (260 and 259 mm, respectively), but rapid testicular growth began earlier in 3/4-Finn rams. The estimated age at which the growth rate in scrotal circumference was maximum was 81 d for 3/4-Finn rams and 93 d for 1/8-Finn rams. No differences were observed in testicular growth in Group 2 rams. However, in Group 3, smaller WF rams matured more rapidly than larger BF rams. Growth in scrotal circumference was most rapid at 99 d in BF rams and 88 d in WF rams. Within WF rams, Barbados Blackbelly X Dorset and Finn X Dorset rams matured earlier than Dorsets. In all groups, the primary breed difference was associated with age differences during the period of rapid testicular growth.  相似文献   
995.
Steers and calves were experimentally infected with bovine leukosis virus. The virus was isolated from the blood and from the tracheal and bronchoalveolar washings before antibodies could be detected in the serum. Bovine leukosis virus was not detected during any period in the blood plasma.  相似文献   
996.
ABSTRACT Wheat was assessed at four crop growth stages for take-all (Gaeumannomyces graminis var. tritici) in a series of field trials that studied the effects of five wheat management practices: sowing date, plant density, nitrogen fertilizer dose and form, and removal/burial of cereal straw. An equation expressing disease level as a function of degree days was fitted to the observed disease levels. This equation was based on take-all epidemiology and depended on two parameters reflecting the importance of the primary and secondary infection cycles, respectively. Early sowing always increased disease frequency via primary infection cycle; its influence on the secondary cycle was variable. Primary infection and earliness of disease onset were increased by high density; however, at mid-season take-all was positively correlated to the root number per plant, which was itself negatively correlated to plant density. At late stages of development, neither plant density nor root number per plant had any influence on disease. A high nitrogen dose increased both take-all on seminal roots and severity of primary infection cycle but decreased take-all on nodal roots and secondary infection cycle. Ammonium (versus ammonium nitrate) fertilizer always decreased disease levels and infection cycles, whereas straw treatment (burial versus removal of straw from the previous cereal crop) had no influence.  相似文献   
997.
Effects of pretreatment of Brassica napus leaves with ascospores of Leptosphaeria biglobosa or chemical defence activators [acibenzolar- S -methyl (ASM) or menadione sodium bisulphite (MSB)] on infection by ascospores of Leptosphaeria maculans (phoma stem canker) and development of disease were studied in controlled-environment (phoma leaf spot) and field (phoma leaf spot and stem canker) experiments. In controlled-environment experiments, pretreatment of oilseed rape leaves (cv. Madrigal) with L. biglobosa , ASM or MSB delayed the appearance of L. maculans phoma leaf spot lesions. These pretreatments also decreased the phoma leaf spot lesion area in both pretreated leaves (local effect) and untreated leaves (systemic effect). In winter oilseed rape field experiments in the 2002/03 and 2003/04 growing seasons, pretreatment with L. biglobosa or ASM in October/November decreased not only the number of phoma leaf spot lesions per leaf caused by L. maculans in autumn/winter, but also the severity of phoma stem canker in the subsequent spring/summer. Effects were greater in 2002/03 (when natural L. maculans ascospore release began in September 2002) than in 2003/04 (when ascospore release began in December following a period of dry weather in August/September 2003). These results suggest that pretreatment with biological or chemical defence activators can induce local and systemic resistance to L. maculans , with both short-term effects on the development of phoma leaf spotting and long-term effects on the development of stem canker 8 months later.  相似文献   
998.
A viral complex causing golden mosaic and leaf distortion (rugosity) in tomato plants was obtained from viruliferous whiteflies, and named TGV-Ub1. This complex was sap-transmitted from tomato to Nicotiana benthamiana . PCR amplification using universal begomovirus primers yielded two distinct fragments for DNA-A, suggesting that the TGV-Ub1 complex comprised at least two distinct viruses. Clones corresponding to full-length viral genomes were obtained from tomato plants infected with TGV-Ub1. Comparisons of the complete sequences of clones pUb1-49 (DNA-A), pUb1-62 and pUb1-81 (both DNA-B) indicated that they constitute novel western hemisphere begomoviruses. Clones pUb1-49 and pUB1-81 have identical common regions, thus representing the cognate DNA-A and -B of a novel begomovirus, named Tomato rugose mosaic virus (ToRMV). Clone pUb1-62 has a distinct common region from ToRMV and all other geminiviruses. A cognate DNA-A for pUb1-62 was not found. Clones containing 1·8 copies of the genomic components were constructed. Infectivity assays of these clones in tomato and N. benthamiana demonstrated that the clones corresponding to ToRMV systemically infected both hosts. Symptoms were analogous to those observed when using the pure isolates obtained in this study. The combination of pUb1-49 and -62 did not result in systemic infection, indicating that these components do not form a viable virus. ToRMV was sap-transmitted from N. benthamiana to N. benthamiana , and by grafting to Solanum tuberosum and Datura stramonium . ToRMV-A and ToRMV-B were detected in plants of Nicandra physaloides and Phaseolus vulgaris , respectively, growing in nearby tomato fields, in association with distinct DNA components.  相似文献   
999.
Data were collected and analysed from seven field sites in Australia, Brazil and Colombia on weather conditions and the severity of anthracnose disease of the tropical pasture legume Stylosanthes scabra caused by Colletotrichum gloeosporioides . Disease severity and weather data were analysed using artificial neural network (ANN) models developed using data from some or all field sites in Australia and/or South America to predict severity at other sites. Three series of models were developed using different weather summaries. Of these, ANN models with weather for the day of disease assessment and the previous 24 h period had the highest prediction success, and models trained on data from all sites within one continent correctly predicted disease severity in the other continent on more than 75% of days; the overall prediction error was 21·9% for the Australian and 22·1% for the South American model. Of the six cross-continent ANN models trained on pooled data for five sites from two continents to predict severity for the remaining sixth site, the model developed without data from Planaltina in Brazil was the most accurate, with >85% prediction success, and the model without Carimagua in Colombia was the least accurate, with only 54% success. In common with multiple regression models, moisture-related variables such as rain, leaf surface wetness and variables that influence moisture availability such as radiation and wind on the day of disease severity assessment or the day before assessment were the most important weather variables in all ANN models. A set of weights from the ANN models was used to calculate the overall risk of anthracnose for the various sites. Sites with high and low anthracnose risk are present in both continents, and weather conditions at centres of diversity in Brazil and Colombia do not appear to be more conducive than conditions in Australia to serious anthracnose development.  相似文献   
1000.
Late blight, caused by Phytophthora infestans , is the most severe disease of potato worldwide. Controlling late blight epidemics is difficult, and resistance of host cultivars is either not effective enough, or too easily overcome by the pathogen to be used alone. In field trials conducted for 3 years under natural epidemics, late blight severity was significantly lower in a susceptible cultivar growing in rows alternating with partially resistant cultivars (mixtures) than in unmixed plots of the susceptible cultivar alone. Partially resistant cultivars behaved similarly in unmixed and mixed plots. Mixtures of cultivars reduced disease progress rates and sometimes delayed disease onset over unmixed plots, but did so significantly only for the slowest epidemic. This suggests that reduction of area under the disease progress curve (AUDPC) in mixtures resulted from the cumulative action of minor effects. Disease distribution was focal in all plots at all dates, as shown by Morisita's index values significantly exceeding 1. Significant yield increases for the susceptible cultivar, and occasionally for the partially resistant ones, were observed in mixed-cultivar plots compared with single-cultivar plots. These results show that cultivar mixtures can significantly reduce natural, polycyclic epidemics in broadleaved plants attacked by pathogens causing rapidly expanding lesions.  相似文献   
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