New Insights of Turmeric Extract-Loaded PLGA Nanoparticles: Development,Characterization and In Vitro Evaluation of Antioxidant Activity     

Gonzales  Camila Maria  Dalmolin  Luciana Facco  da Silva  Kátia Aparecida  Slade  Natália Bueno Leite  Lopez  Renata Fonseca Vianna  Moreto  Jeferson Aparecido  Schwarz  Kélin 《Plant foods for human nutrition (Dordrecht, Netherlands)》2021,76(4):507-515

Here, we presented new insights of the development of poly(lactic-co-glycolic acid) nanoparticles containing turmeric compounds (turmeric-PLGA-NPs) using emulsion-solvent evaporation method. The nanoparticulate system was characterized by size, zeta potential, morphology, release profile, partition parameter, stability and encapsulation efficiency (%EE). Antioxidant activity studies were also evaluated. The Korsmeyer-Peppas model (M_t/M_∞ vs. t) was used to determine the release mechanisms of the studied system. Our results demonstrated the emulsion-solvent evaporation method was shown advantageous for producing turmeric-PLGA-NPs in the range of 145 nm with high homogeneity in size distribution, zeta potential of ?21.8 mV and %EE about 72%. Nanoparticles were stable over a period of one month. In vitro study showed a release of curcumin governed by diffusion and relaxation of the polymeric matrix. The partition parameter of the extract in relation to blank-PLGA-NPs was 0.111?±?0.008 M^?1, indicating a low affinity of curcumin for the polymer matrix. Antioxidant ability of the turmeric-PLGA-NPs in scavenging the radical 2,2-azinobis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) was inferior to free turmeric extract and showed a concentration and time-dependent profile. The study concluded that PLGA nanoparticles are potential carriers for turmeric extract delivery.

  相似文献   

Characterization of “Mais delle Fiorine” (Zea mays L.) and nutritional,morphometric and genetic comparison with other maize landraces of Lombardy region (Northern Italy)     

Giupponi  Luca  Leoni  Valeria  Colombo  Federico  Cassani  Elena  Hejna  Monika  Rossi  Luciana  Pilu  Roberto 《Genetic Resources and Crop Evolution》2021,68(5):2075-2091

Genetic Resources and Crop Evolution - The loss of agrobiodiversity is a topic of global impact. On a local scale, Lombardy, in the Alpine macro-Region, has lost more than 78% of its plant...  相似文献   

Review of the Book Bayesian Analysis Made Simple: An Excel GUI for WinBUGS,by P. Woodward     

Luciana Dalla Valle 《Journal of Agricultural, Biological & Environmental Statistics》2014,19(1):156-157

  相似文献   

Differences in leaf yield and indigo precursors production in woad (Isatis tinctoria L.) and Chinese woad (Isatis indigotica Fort.) genotypes     

Luciana G. Angelini  Sabrina TozziNicoletta Nassi o Di Nasso 《Field Crops Research》2007

Isatis tinctoria L. (woad) is one of the earliest known sources of indigo in Europe where it was cultivated since the Middle Ages. Isatisindigotica Fort. (Chinese woad), widely distributed in China, had been used from ancient times as indigo-producing plant and medicinal plant. Both species produce indigo precursors indican (indoxyl β-d glucoside) and isatan B (indoxyl ketogluconate) in their leaves. In order to identify new suitable crops for indigo production in Italy, 17 woad lines were studied under field conditions in Central Italy (Pisa, 43°40′N, 10°19′E) from 2001 to 2003. We analyzed the effects of year, genotype, and harvest times together with their reciprocal interactions on leaf yield and indigo precursors production. Woad lines were then compared with seven I. indigotica lines in a field crop experiment set up in 2003. Extraction and quantification of indigo precursors were accomplished by HPLC-ELSD. Isatan B and indican content, as well as equivalent indigo and fresh/dry leaf yield, were compared between species and among genotypes.  相似文献   

Matrix dominance and landscape resistance affect genetic variability and differentiation of an Atlantic Forest pioneer tree     

Silveira dos Santos  Juliana  Vitorino  Luciana Cristina  Fabrega Gonçalves  Renata  Corrêa Côrtes  Marina  Souza Cruz Alves  Rafael  Cezar Ribeiro  Milton  Garcia Collevatti  Rosane 《Landscape Ecology》2022,37(10):2481-2501

Landscape Ecology - Despite the importance of secondary forests for the maintenance of biodiversity, the impact of pioneer trees on habitat loss and fragmentation is poorly understood. We analyzed...  相似文献   

Assessment of blood types of Ragdoll cats for transfusion purposes     

Daniela Proverbio  Eva Spada  Roberta Perego  Alessandra Della Pepa  Giada Bagnagatti De Giorgi  Luciana Baggiani 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2013,42(2):157-162

  相似文献   

Effect of Astaxanthin on Human Sperm Capacitation     

Gabriella Donà  Ivana Ko?uh  Anna Maria Brunati  Alessandra Andrisani  Guido Ambrosini  Guglielmo Bonanni  Eugenio Ragazzi  Decio Armanini  Giulio Clari  Luciana Bordin 《Marine drugs》2013,11(6):1909-1919

In order to be able to fertilize oocytes, human sperm must undergo a series of morphological and structural alterations, known as capacitation. It has been shown that the production of endogenous sperm reactive oxygen species (ROS) plays a key role in causing cells to undergo a massive acrosome reaction (AR). Astaxanthin (Asta), a photo-protective red pigment belonging to the carotenoid family, is recognized as having anti-oxidant, anti-cancer, anti-diabetic and anti-inflammatory properties and is present in many dietary supplements. This study evaluates the effect of Asta in a capacitating buffer which induces low ROS production and low percentages of acrosome-reacted cells (ARC). Sperm cells were incubated in the presence or absence of increasing concentrations of Asta or diamide (Diam) and analyzed for their ROS production, Tyr-phosphorylation (Tyr-P) pattern and percentages of ARC and non-viable cells (NVC). Results show that Asta ameliorated both sperm head Tyr-P and ARC values without affecting the ROS generation curve, whereas Diam succeeded in enhancing the Tyr-P level but only of the flagellum without increasing ARC values. It is suggested that Asta can be inserted in the membrane and therefore create capacitation-like membrane alteration which allow Tyr-P of the head. Once this has occurred, AR can take place and involves a higher numbers of cells.  相似文献   

Concentrations of platelet α2-adrenoceptors,lymphocyte muscarinic receptors,and blood monoamines in dogs (Canis familiaris) affected by canine cognitive dysfunction syndrome     

Paola Badino  Rosangela Odore  Luciana Bergamasco  Raffaella Barbero  Maria Cristina Osella  Antonio D'Angelo  Giovanni Re  Carlo Girardi 《Journal of Veterinary Behavior: Clinical Applications and Research》2013,8(3):146-153

Canine cognitive dysfunction syndrome (CDS) is a neurodegenerative disorder of aged dogs characterized by a progressive decline in cognitive function. In humans and laboratory animals, a variety of neurotransmitter abnormalities have been described in patients affected by age-related dementia. Specifically, the regulatory role of the catecholaminergic, serotonergic, and cholinergic systems has been outlined. The aim of the present study was to measure blood monoamine levels, platelet α₂-adrenergic receptors, and lymphocyte muscarinic receptors in healthy adult and aged dogs and in dogs affected by canine cognitive dysfunction. Based on clinical and behavioral examination, 40 dogs were divided into 3 groups: healthy adults (n = 14), aged dogs (n = 17), and aged dogs affected by canine cognitive dysfunction (n = 9). A significant reduction in plasma levels of norepinephrine and dopamine was observed both in aged dogs (0.16 ± 0.02 ng/mL, P < 0.01; 0.11 ± 02 ng/mL, P < 0.01, respectively) and in CDS dogs (0.14 ± 0.03 ng/mL, P < 0.05; 0.10 ± 00.005 ng/mL, P < 0.01, respectively) compared with adults (0.29 ± 0.04 ng/mL and 0.15 ± 0.02 ng/mL, respectively). No significant differences were observed among groups for α₂-adrenergic receptor concentrations. Canine lymphocytes express 2 distinct classes of muscarinic receptors, characterized by high (HA) and low affinity (LA) for [³H]-N-methyl-scopolamine. A significant age-dependent decrease in HA muscarinic receptors was observed. However, no differences were found between aged dogs (87.65 ± 11.08 sites/cell × 10²) and in CDS dogs (90.17 ± 6.75 sites/cell × 10² ) for HA muscarinic receptor concentrations. As far as LA muscarinic receptors are concerned, CDS dogs showed a significant increase (393.48 ± 63 sites/cell × 10²; P < 0.05) with respect to healthy adult dogs (188.84 ± 16.50 sites/cell × 10²). Our results suggest that the reduction in HA muscarinic receptor-binding sites could be representative of the physiological aging process, whereas the increase in lymphocyte LA muscarinic receptor levels could be related to the cognitive decline.  相似文献   

Kinetics and Adsorption Isotherms of Bisphenol A, Estrone, 17β-Estradiol, and 17α-Ethinylestradiol in Tropical Sediment Samples     

Bruno B. Cunha  Wander Gustavo Botero  Luciana Camargo Oliveira  Viviane M. Carlos  Marcelo L. M. Pompêo  Leonardo F. Fraceto  André H. Rosa 《Water, air, and soil pollution》2012,223(1):329-336

The sorption of four endocrine disruptors, bisphenol A (BPA), estrone (E1), 17β-estradiol (E2), and 17α-ethinylestradiol (EE2) in tropical sediment samples was studied in batch mode under different conditions of pH, time, and sediment amount. Data obtained from sorption experiments using the endocrine disruptors (EDs) and sediments containing different amounts of organic matter showed that there was a greater interaction between the EDs and organic matter (OM) present in the sediment, particularly at lower pH values. The pseudosecond order kinetics model successfully explained the interaction between the EDs and the sediment samples. The theoretical and experimentally obtained q _e values were similar, and k values were smaller for higher SOM contents. The k _F values, obtained from the Freundlich isotherms, varied in the ranges 4.2–7.4 × 10⁻² (higher OM sediment sample, S₂) and 1.7 × 10⁻³–3.1 × 10⁻² (lower OM sediment sample, S₁), the latter case indicating an interaction with the sediment that increased in the order: EE2 > > E2 > E1 > BPA. These results demonstrate that the availability of endocrine disruptors may be directly related to the presence of organic material in sediment samples. Studies of this kind provide an important means of understanding the mobility, transport, and/or reactivity of this type of emergent contaminant in aquatic systems.  相似文献   

Long-term erosion of tree reproductive trait diversity in edge-dominated Atlantic forest fragments     

Ariadna Valentina Lopes  Luciana Coe Girão  Carlos A. Peres 《Biological conservation》2009,142(6):1154-1997

Habitat loss and fragmentation promote relatively predicable shifts in the functional signature of tropical forest tree assemblages, but the full extent of cascading effects to biodiversity persistence remains poorly understood. Here we test the hypotheses that habitat fragmentation (a) alters the relative contribution of tree species exhibiting different reproductive traits; (b) reduces the diversity of pollination systems; and (c) facilitates the functional convergence of reproductive traits between edge-affected and early-secondary forest habitats (5-32 years old). This study was carried out in a severely fragmented 670-km² forest landscape of the Atlantic forest of northeastern Brazil. We assigned 35 categories of reproductive traits to 3552 trees (DBH ? 10 cm) belonging to 179 species, which described their pollination system, floral biology, and sexual system. Trait abundance was calculated for 55 plots of 0.1 ha across four habitats: forest edges, small forest fragments (3.4-83.6 ha), second-growth patches, and core tracts of forest interior within the largest available primary forest fragment (3500 ha) in the region. Edge-affected and secondary habitats showed a species-poor assemblage of trees exhibiting particular pollination systems, a reduced diversity of pollination systems, a higher abundance of reproductive traits associated with pollination by generalist diurnal vectors, and an elevated abundance of hermaphroditic trees. As expected, the reproductive signature of tree assemblages in forest edges and small fragments (edge-affected habitats), which was very similar to that of early second-growth patches, was greatly affected by both habitat type and plot distance to the nearest forest edge. In hyper-fragmented Atlantic forest landscapes, we predict that narrow forest corridors and small fragments will become increasingly dominated by edge-affected habitats that can no longer retain the full complement of tree life-history diversity and its attendant mutualists.  相似文献