Inactivation of avian influenza virus using common detergents and chemicals     

Lombardi ME  Ladman BS  Alphin RL  Benson ER 《Avian diseases》2008,52(1):118-123

Six disinfectant chemicals were tested individually for effectiveness against low pathogenic avian influenza virus (LPAIV) A/H7N2/Chick/MinhMa/04. The tested agents included acetic acid (C2H4O2), citric acid (C6H8O7), calcium hypochlorite (Ca(ClO)2), sodium hypochlorite (NaOCl), a powdered laundry detergent with peroxygen (bleach), and a commercially available iodine/acid disinfectant. Four of the six chemicals, including acetic acid (5%), citric acid (1% and 3%), calcium hypochlorite (750 ppm), and sodium hypochlorite (750 ppm) effectively inactivated LPAIV on hard and nonporous surfaces. The conventional laundry detergent was tested at multiple concentrations and found to be suitable for inactivating LPAIV on hard and nonporous surfaces at 6 g/L. Only citric acid and commercially available iodine/acid disinfectant were found to be effective at inactivating LPAIV on both porous and nonporous surfaces.  相似文献   

Biodegradation combined with ozone for the remediation of contaminated soils     

Marco Derudi  Gianluca Venturini  Giorgio Lombardi  Giuseppe Nano  Renato Rota   《European Journal of Soil Biology》2007,43(5-6):297

The effectiveness of the SS-SBR (Soil Slurry – Sequencing Batch Reactor) process for the remediation of soils contaminated by several organic pollutants has been evaluated. Experimental tests have been performed on two different soils, a spiked one and an industrial aged soil. The spiked soil, artificially contaminated, has been prepared trying to simulate the pollution of an industrial aged soil in terms of number and kind of contaminants. PAHs (Polycyclic Aromatic Hydrocarbons) and phenols degradation has been particularly investigated because they are considered persistent and recalcitrant. Concerning the spiked soil, removal efficiencies higher than 95% in 6 to 9 weeks have been found for all the pollutants, except for five-rings PAHs; however, these compounds were partly removed in 11 to 13 weeks. Good results have been achieved also for the industrial aged soil with a maximum removal of about 80% in 7–8 weeks. To enhance the pollutants degradation, trying to obtain a faster remediation, the biological treatment has been combined with a chemical oxidation with ozone. The best degradation effectiveness of the combined process has been obtained applying the ozonation after few days of the biological treatment. Therefore, a combined biological and chemical treatment allowed to markedly improve the remediation of contaminated soils.  相似文献   

Down-regulation of LFA-1 adhesion receptors by C-myc oncogene in human B lymphoblastoid cells   总被引：16，自引：0，他引：16  

G Inghirami  F Grignani  L Sternas  L Lombardi  D M Knowles  R Dalla-Favera 《Science (New York, N.Y.)》1990,250(4981):682-686

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Disruption of the human SCL locus by "illegitimate" V-(D)-J recombinase activity   总被引：32，自引：0，他引：32  

P D Aplan  D P Lombardi  A M Ginsberg  J Cossman  V L Bertness  I R Kirsch 《Science (New York, N.Y.)》1990,250(4986):1426-1429

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Resistance to classical scrapie in experimentally challenged goats carrying mutation K222 of the prion protein gene     

Acutis PL  Martucci F  D'Angelo A  Peletto S  Colussi S  Maurella C  Porcario C  Iulini B  Mazza M  Dell'atti L  Zuccon F  Corona C  Martinelli N  Casalone C  Caramelli M  Lombardi G 《Veterinary research》2012,43(1):8

ABSTRACT: Susceptibility of sheep to scrapie, a transmissible spongiform encephalopathy of small ruminants, is strongly influenced by polymorphisms of the prion protein gene (PRNP). Breeding programs have been implemented to increase scrapie resistance in sheep populations; though desirable, a similar approach has not yet been applied in goats. European studies have now suggested that several polymorphisms can modulate scrapie susceptibility in goats: in particular, PRNP variant K222 has been associated with resistance in case-control studies in Italy, France and Greece. In this study we investigated the resistance conferred by this variant using a natural Italian goat scrapie isolate to intracerebrally challenge five goats carrying genotype Q/Q 222 (wild type) and five goats carrying genotype Q/K 222. By the end of the study, all five Q/Q 222 goats had died of scrapie after a mean incubation period of 19 months; one of the five Q/K 222 goats died after 24 months, while the other four were alive and apparently healthy up to the end of the study at 4.5 years post-challenge. All five of these animals were found to be scrapie negative. Statistical analysis showed that the probability of survival of the Q/K 222 goats versus the Q/Q 222 goats was significantly higher (p = 0.002). Our study shows that PRNP gene mutation K222 is strongly associated with resistance to classical scrapie also in experimental conditions, making it a potentially positive target for selection in the frame of breeding programs for resistance to classical scrapie in goats.  相似文献   

Antioxidant compounds and antioxidant activity in "early potatoes"   总被引：1，自引：0，他引：1  

Leo L  Leone A  Longo C  Lombardi DA  Raimo F  Zacheo G 《Journal of agricultural and food chemistry》2008,56(11):4154-4163

The antioxidant content and the antioxidant capacity of both hydrophilic and lipophilic antioxidant extracts from four "early potato" cultivars, grown in two different locations (Racale and Monteroni), were examined. There was a considerable variation in carotenoid content and weak differences in the ascorbic acid concentration of the examined cultivars of "early potato" and between the harvested locations. An increase in both methanol/water (8:2 v/v) and phosphate buffer soluble (PBS) free phenols (70%) and bound phenols (28%) in the extracts from the cultivars grown at Racale site was found and discussed. Examination of individual phenols revealed that chlorogenic acid and catechin were the major phenols present in potato tuber extracts; a moderate amount of caffeic acid and ferulic acid was also detected. The total equivalent antioxidant capacity (TEAC) was higher in the Racale extracts and a highly positive linear relationship ( R (2) = 0.8193) between TEAC values and total phenolic content was observed. The oxyradical scavenging capacity (TOSC) of methanol/water and PBS extracts of peel and whole potatoes against the reactive oxygen species (ROS) peroxyl radicals, peroxynitrite, and hydroxyl radicals was also analyzed. A highly significant linear correlation ( R (2) = 0.9613) between total antioxidant capacity (as a sum of peroxyl radicals + peroxynitrite) and total phenol content of methanol/water extracts was established. Moreover, proliferation of human mammalian cancer (MCF-7) cells was significantly inhibited in a dose-dependent manner after exposure to potato extracts. These data can be useful for "early potato" tuber characterization and suggest that the "early potato" has a potential as a dietary source of antioxidants.  相似文献   

In vitro permeation through porcine buccal mucosa of caffeic acid phenetyl ester (CAPE) from a topical mucoadhesive gel containing propolis     

Ceschel GC  Maffei P  Sforzini A  Lombardi Borgia S  Yasin A  Ronchi C 《Fitoterapia》2002,73(Z1):S44-S52

Recent studies have shown that propolis has on the oral cavity appreciable antibacterial, antifungal and antiviral actions, as well as anti-inflammatory, anaesthetic and cytostatic properties. In light of these studies, an assessment of the diffusion and permeation of caffeic acid phenetyl ester (CAPE) through porcine buccal mucosa was considered useful as a possible application in the stomatological field. To do so, a mucoadhesive topical gel was prepared to apply to the buccal mucosa. The gel was formulated in such a way as to improve the solubility of the propolis, conducting to an increase of the flux. The mucosal permeation of CAPE from the formulation was evaluated using Franz cells, with porcine buccal mucosa as septum between the formulation (donor compartment) and the receptor phase chamber. The diffusion through the membrane was determined by evaluating the amount of CAPE present in the receiving solution, the flux and the permeation coefficient (at the steady state) in the different formulations at set intervals. Qualitative and quantitative determinations were done by HPLC analysis. From the results, CAPE allowed a high permeability coefficient in comparison to the coefficient of other molecules, as expected from its physical-chemical structure. Moreover, the developed gel improved the CAPE flux approximately 35 times more with respect to an ethanol solution formulated at the same gel concentration. The developed gel was also tested in order to evaluate the mucoadhesive behaviour and comfort in vivo on 10 volunteers in a period of 8 h. The in vivo evaluation of mucoadhesive gel revealed adequate comfort and non-irritancy during the period of study and it was well accepted by the volunteers.  相似文献   

Angiotensin-converting enzyme inhibition by Brazilian plants   总被引：1，自引：0，他引：1  

Braga FC  Serra CP  Viana NS  Oliveira AB  Côrtes SF  Lombardi JA 《Fitoterapia》2007,78(5):353-358

The potential antihypertensive activity of Brazilian plants was evaluated in vitro by its ability to inhibit the angiotensin-converting enzyme (ACE). Forty-four plants belonging to 30 families were investigated. Plants were selected based on their popular use as antihypertensive and/or diuretics. The following plants presented significant ACE inhibition rates: Calophyllum brasiliense, Combretum fruticosum, Leea rubra, Phoenix roebelinii and Terminalia catappa.  相似文献   

Effect of Lactobacillus plantarum on growth performance and hematological traits of European sea bass (Dicentrarchus labrax)     

Giovanni Piccolo  Fulvia Bovera  Pietro Lombardi  Vincenzo Mastellone  Sandra Nizza  Carmelo Di Meo  Stefania Marono  Antonino Nizza 《Aquaculture International》2015,23(4):1025-1032

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Pharmacokinetics of tilmicosin in beef cattle following intravenous and subcutaneous administration     

Lombardi KR  Portillo T  Hassfurther R  Hunter RP 《Journal of veterinary pharmacology and therapeutics》2011,34(6):583-587

The intravenous pharmacokinetic profile of tilmicosin is yet to be achieved because of the cardiovascular effects of tilmicosin. This study summarizes two pharmacokinetic studies that provided complete pharmacokinetic profile of tilmicosin in cattle. The first study was a pharmacokinetic study of tilmicosin in beef calves dosed by i.v. infusion over 5 h. The second study was a subcutaneous (s.c.) pharmacokinetic study comparing the pharmacokinetic profile of tilmicosin in light (approximately 170 kg) and heavy (approximately 335 kg) beef cattle and comparing the labeled dose range of 10 or 20 mg/kg dose. The data from the two different studies were used to calculate bioavailability values, which support the assumption that tilmicosin is 100% bioavailable in cattle. The results from the second study showed that the weight of an animal when administered tilmicosin does not have a significant effect on exposure, but did demonstrate that doubling the dose of tilmicosin administered doubles the systemic exposure to tilmicosin.  相似文献