Behavior,blood stress indicators,skin lesions,and meat quality in pigs transported to slaughter at different loading densities     

Valentina Montoya Urrea  Ana Maria Bridi  Maria Camila Ceballos  Mateus J R Paranhos da Costa  Luigi Faucitano 《Journal of animal science》2021,99(6)

A total of 1,936 pigs were transported through 8 trips (8.4 ± 0.5 hr) from 2 grow-to-finish farms to a commercial slaughter plant, all located in Southern Brazil. On each trip, a sub-sample of each load (36 barrows/load, weighing 118.9 ± 9.8 kg) was randomly allocated into one of the following loading densities during transport: 200 kg/m² (D200), 235 kg/m² (D235), and 270 kg/m² (D270). Behavioral recordings of postures and activities were made during transportation and lairage using video-cameras. At slaughter, blood samples were collected to assess the concentrations of creatine kinase (CK) and lactate. Carcass weights and skin lesion scores were assessed on-line, and meat quality was evaluated in the longissimus thoracis (LT) muscle by assessing pH, color, and drip loss. During transportation, the proportion of animals lying down was higher (P < 0.05) in D200 and D235 groups compared with D270. The proportion of sitting animals during transportation was higher (P < 0.01) in D270 compared with D200. In lairage, D200 and D235 pigs stood more compared with D270 (P = 0.01), while the proportion of lying pigs was higher (P = 0.02) for D270 pigs compared with D200 and D235. The frequency of drinking bouts in lairage was higher (P < 0.05) for D200 group compared with D235 and D270. The levels of CK were lower (P < 0.05) in D200 pigs transported compared with D270. Lesion scores tended to be higher (P = 0.06) in D270 carcasses compared with D200 and D235. A tendency for lower (P = 0.10) pH1 values in the LT muscle of D270 pigs compared with D200 pigs was also found. Based on the results of this study, the application of lower loading densities (≤235 kg/m²) in the truck allows pigs to have sufficient space to rest, travel more comfortably and arrive less fatigued at the slaughter plant.  相似文献   

Effect of antioxidant protection of must on volatile compounds and aroma shelf life of Falanghina (Vitis vinifera L.) wine     

Moio L  Ugliano M  Genovese A  Gambuti A  Pessina R  Piombino P 《Journal of agricultural and food chemistry》2004,52(4):891-897

Two vinification methods involving different degrees of antioxidant protection of Falanghina must during prefermentative steps, and referred as HAMP (high antioxidant must protection) and LAMP (low antioxidant must protection), were compared in terms of fermentation performances of four different yeast strains, composition of the volatile fraction of wines at the end of alcoholic fermentation, and shelf life of wines during storage. The use of HAMP technology resulted in wines with lower volatile acidity and higher concentrations of medium-chain fatty acid ethyl esters, acetates, and volatile fatty acids. For two of the four strains a lower concentration of isoamyl alcohol was also observed. HAMP wines also revealed increased shelf life because of the higher concentration of odor active esters at the end of storage and better preservation of varietal aromas.  相似文献   

Influence of the Fertilisation System on the Mechanical Damage of Apples   总被引：1，自引：1，他引：1  

L Pasini  L Ragni  A.D Rombol  A Berardinelli  A Guarnieri  B Marangoni 《Biosystems Engineering》2004,88(4):441-452

Fuji and Gala apples obtained with different fertilisation systems (soil surface application and fertigation) were submitted to drop tests to analyse the possible difference in susceptibility to damage. The tests were carried out by simulating two impacts of different severity that occur in a packing line. For the Fuji apples, a test was also carried out to assess the effect of a long storage period. For the test with the highest impact severity, the apple acceleration was recorded to describe the mechanical behaviour of the fruit and to correlate it with the damage. Logistic and multiple linear regressions were performed to assess the correlations between probability, extent and nature of damage, fertilisation systems and main characteristics of the fruits. The results show that the resistance to damage of Fuji and Gala apples is influenced by the fertilisation system both in probability and in characteristics of damage. Duration of storage can invert the susceptibility to damage, with the apples having a higher resistance to damage after a longer period of storage. Conventional fertilisation induced a high susceptibility to darkening and fracturing of the apples tissue. The parameters to describe the mechanical behaviour of apples were often correlated with the fertilisation system. However, their influence on extent and nature of damage was not significant or substantial while they can have a high effect on probability of damage. Fuji apples were found to be more susceptible to damage than Gala apples.  相似文献   

Molecular and physiological characterisation of psychrotrophic hydrocarbon-degrading bacteria isolated from Terra Nova Bay (Antarctica)     

Francesco Pini  Cristina Grossi  Sabrina Nereo  Luigi Michaud  Angelina Lo Giudice  Vivia Bruni  Franco Baldi  Renato Fani   《European Journal of Soil Biology》2007,43(5-6):368

A set of 21 Antarctic marine bacteria isolated from the Ross Sea and able to utilise diesel fuel as the sole carbon and energy source was characterised. Isolates were analysed by amplified 16S rDNA restriction analysis using the enzyme AluI, resulting in two different groups corresponding to different bacterial species. These species were assigned to the genera Rhodococcus and Alcaligenes, on the basis of 16S rDNA sequencing. This low degree of inter-specific biodiversity was parallel to a low intra-specific biodiversity, as shown by Random Amplified Polymorphic DNA analysis. Then, a 550-bp DNA fragment coding for the inner region of alkane mono-oxygenase was PCR-amplified from the genome of each strain. The phylogenetic analysis of the sequence of the putative AlkB protein coded for by the amplified DNA fragment revealed that these alkB genes were very likely inherited by horizontal gene transfer. Lastly, the analysis of the biodegradation ability of four strains revealed two different strategies of hydrocarbon uptake, mediated either by bio-surfactants and peculiar of Rhodococcus isolates, or by membrane modifications and shown by Alcaligenes isolates. In order to understand the interrelationships between hydrocarbon-degrading isolates, the dynamics of two strains, belonging to Rhodococcus and Alcaligenes, grown together in a co-culture was also followed over a seventeen days period.  相似文献   

Effect of ochratoxin A-producing Aspergilli on stilbenic phytoalexin synthesis in grapes     

Bavaresco L  Vezzulli S  Battilani P  Giorni P  Pietri A  Bertuzzi T 《Journal of agricultural and food chemistry》2003,51(21):6151-6157

Berries of Vitis vinifera L. cv. Barbera were infected, at veraison and during ripening, by a conidial suspension of A. japonicus, A. ochraceus, A. fumigatus and two isolates of A. carbonarius to control ochratoxin A production and stilbene induced synthesis. The experimental design provided also for intact and punctured berries and incubation temperature of 25 degrees C and 30 degrees C. All the tested fungi, except A. fumigatus, significantly increased trans-resveratrol synthesis over the control, while trans-piceid was not affected; only A. ochraceus significantly elicited the berries to synthesize piceatannol. The two isolates of A. carbonarius produced higher amounts of ochratoxin A than did the other fungi. A positive correlation between ochratoxin A and trans-resveratrol synthesis occurred. trans-Resveratrol and piceatannol showed fungicidal activity against A. carbonarius, being able to completely inhibit fungal growth at a concentration of 300 microg/g and 20 microg/g, respectively.  相似文献   

Variation within improved cultivars and landraces of lucerne in Central Italy     

Falcinelli  Mario  Russi  Luigi  Negri  Valeria  Veronesi  Fabio 《Euphytica》1994,77(3):199-203

Summary A variety must be distinguishable, uniform, stable and of sufficient productivity to be officially registered. In Italy landraces of lucerne are still widely used because they are characterized by a good persistence and productivity in their area of origin and adaptation.The landrace from Casalina (20 km south of Perugia, Central Italy) has higher dry matter production than many other commercial cultivars. The objective of this paper was to measure the variation of the landrace Casalina and compare it with that of the commonest cultivars available on the market and with some lines selected from the landrace Casalina.The experiment consisted of 11 entries: Casalina, two selections from Casalina (one for high seed yield and one for frequent cuttings), the registered landrace Italia Centrale and 7 registered cultivars. Each entry was represented by 80 genotypes transplanted in the field 60×40 cm apart in a randomized block design with 4 replications. In 1991 the following characters were recorded on a single plant basis: time of first flowering, height, number of shoots and dry matter yield at the first harvest, time of second flowering, leafiness, height, number of shoots and dry matter yield at the second harvest, height, number of shoots and dry matter yield at the third and fourth harvest. Height, number of shoots and dry matter yield at the first harvest, dry matter at the second and third harvest were recorded during the 1992 season.Casalina was as variable as the other entries in 77% of the comparisons; it was more variable in 8% of the comparisons and less variable in 15% of the comparisons. In conclusion, the variation of landrace Casalina is equal to or less than that of registered varieties so that it could be directly registered at the National Registry of Varieties.  相似文献   

Midazolam oxidation in cattle liver microsomes: The role of cytochrome P450 3A     

Alberto Nassi  Luigi Quintieri  Roberta Merlanti  Francesca Pezzato  Francesca Capolongo  Marianna Pauletto  Mauro Dacasto  Mery Giantin 《Journal of veterinary pharmacology and therapeutics》2020,43(6):608-613

In humans, the cytochrome P450 3A (CYP3A) subfamily is involved in midazolam (MDZ) biotransformation into 1′- and 4-hydroxy metabolites, and the former serves as a probe for CYP3A catalytic activity. In veterinary species is still crucial to identify enzyme- and species-specific CYP substrates; thus, the aim of this study was to characterize MDZ oxidation in cattle liver. A HPLC-UV method was used to measure 1′- and 4-hydroxy MDZ (1′- and 4-OHMDZ, respectively) formation in cattle liver microsomes and assess the role of CYP3A by an immunoinhibition study. Moreover, MDZ hydroxylation was evaluated in 300 cattle liver samples and results were correlated with testosterone hydroxylation. Formation of both metabolites conformed to a single-enzyme Michaelis–Menten kinetics. Values of V_max and K_m were 0.67 nmol/min/mg protein and 6.16 μM for 4-OHMDZ, and 0.06 nmol/min/mg protein and 10.08 μM for 1′-OHMDZ. An anti-rat CYP3A1 polyclonal antibody inhibited up to 50% and 94% 1′- and 4-OHMDZ formation, respectively. MDZ oxidation in liver microsomes was poorly correlated with testosterone hydroxylation. In conclusion, cattle metabolized MDZ to 1′-OHMDZ and 4-OHMDZ. The immunoinhibition results indicated a major contribution of CYP3As to 4-OHMDZ formation and the involvement of other CYPs in 1′-OHMDZ production, paving the way for further investigations.  相似文献   

Wood properties of young Douglas-fir in Southern Italy: results over a 12-year post-thinning period   总被引：1，自引：1，他引：0  

Luigi Todaro  Nicola Macchioni 《European Journal of Forest Research》2011,130(2):251-261

This paper describes the study of a 31-year-old Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) stand located in Southern Italy, which was thinned 19 years after planting. The aim of the study is to evaluate the influence of three thinning treatments (unthinned, selective, and geometrical) performed twelve years later on wood density (WD), moisture content, shrinkage, ring width (RW), latewood proportion (LW%), modulus of elasticity, compression (CS), and bending strength (BS). The WD was higher in the unthinned stand. LW% and BS were lowest in the selective thinning and in the geometrical thinning, respectively. No significant differences were found in other variables. In addition, the thinning processes mostly affected the medium tree class more than the dominant and suppressed ones. Regression analysis established a correlation between mechanical characteristics and WD, RW and LW%. Mechanical strength is strongly correlated with WD than other variables. The stepwise model showed that WD and RW are most closely related to the behavior of CS, whereas only WD explained variation in BS. Stand density reduction may improve the development of stands without greatly affecting wood quality.  相似文献   

Effect of moisture on physical parameters of timber from Turkey oak (<Emphasis Type="Italic">Quercus cerris</Emphasis> L.) coppice in Central Italy     

Angela LO MONACO  Luigi TODARO  Mara SARLATTO  Raffaello SPINA  Luca CALIENNO  Rodolfo PICCHIO 《中国林学(英文版)》2011,13(4):276-284

This study aims to investigate wood density at different levels of moisture, basic density and shrinkage of timber from Turkey oak (Quercus cerris L.) coppice forests growing in Central Italy. We also studied the variability in density in the trees within and among sites. Density shows no significant statistical differences in the tested population. A higher variability in the shrinkage than in the density was found. Wood moisture is referred to as dry mass and fresh mass, which is related to many performance characteristics of wood, i.e., energy production. Trends in moisture and water content were studied because these physical parameters play an important role in the specific area of firewood which requires an accurate estimation of mass, volume and energy content. This work is a contribution to improve xylo-energy estimates of small and medium forestry issues.  相似文献   

DNA fingerprinting and quality traits of Corbarino cherry-like tomato landraces     

Andreakis N  Giordano I  Pentangelo A  Fogliano V  Graziani G  Monti LM  Rao R 《Journal of agricultural and food chemistry》2004,52(11):3366-3371

Twenty-five landraces of the cherry-like tomato named Corbarino, a typical niche product grown in the Sarno valley of the Campania region, have been characterized. The landraces used have been compared to eight cultivars widely spread in the same area of cultivation. The genetic diversity within and between landraces was evaluated through the comparison of DNA fingerprints obtained with (GATA)(4) probe hybridized to TaqI digested genomic DNA. Twenty-two of the Corbarino landraces were homogeneous and were unequivocally characterized by their DNA fingerprints. The others are probably "population varieties" in that within each of them polymorphic DNA fragments were identified. According to the characterization of fruit shapes, four groups were identified. One landrace from each group, harvested at the same ripening degree, was further characterized for yield, fruit quality components, antioxidant activities, and carotenoid contents and compared to Faino F(1) and Tomito F(1) hybrids. The Corbarino landraces were all characterized by high yield, a high level of carbohydrates, and high levels of soluble and total solids. Interestingly, the biotype named ISCI 05 was the most suitable for canning. Landraces ISCI 07 and ISCI 05 gave the highest value of antioxidant activities and carotenoid content. These data, together with previously published results, suggested that ISCI 05 should be proposed for the institution of a Label of Origin.  相似文献