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81.
The development and integrity of equine pre‐antral follicles cultured in vitro with follicle‐stimulating hormone (FSH) supplementation 
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下载免费PDF全文 MC Max CB Silva SM González AG Lindquist I Búfalo RG Gomes F Morotti CB Costa TRR Barreiros LA Lisboa MM Seneda 《Reproduction in domestic animals》2017,52(5):899-904
This study investigated the effects of different concentrations of FSH (10, 50, 100 and 200 ng/ml) in supplemented MEM+ on the development of equine pre‐antral follicles that were cultured in vitro for 2 or 6 days. The ovaries (n = 5) from mares in seasonal anoestrus were collected from a local abattoir. Ten ovarian tissue fragments of approximately 3 × 3 × 1 mm were obtained from each animal. The fragments were cultured in situ for 2 days (D2) or 6 days (D6) in MEM+ or MEM+ supplemented with FSH at four different concentrations, establishing the following 11 groups: control (D0); MEM + (D2); MEM + (D6); MEM + 10 ng/ml of FSH (D2); MEM + 10 ng/ml of FSH (D6); MEM + 50 ng/ml of FSH (D2); MEM + 50 ng/ml of FSH (D6); MEM + 100 ng/ml of FSH (D2); MEM + 100 ng/ml of FSH (D6); MEM + 200 ng/ml of FSH (D2); and MEM + 200 ng/ml of FSH (D6). Follicles were observed in only 9.65% (388 of 4,018) of the histological sections. Of the 861 follicles evaluated, 488 were in the primordial stage, and 373 were in various developmental stages; 59.7% were morphologically normal. Regarding the integrity of the pre‐antral follicles, the groups with 100 ng/ml FSH of 2‐days culture as well as 50, 100 and 200 ng/ml FSH of 6‐days culture provided the best results. In conclusion, the in vitro culture of abattoir‐derived equine ovarian fragments presented better morphological integrity when supplemented with FSH for 6 days, in comparison with the MEM culture group. However, no clear effects were observed with FSH regarding the promotion of activation from a primordial to a developing follicle. 相似文献
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83.
S Chen RS Ingram MJ Hostetler JJ Pietron RW Murray TG Schaaff JT Khoury MM Alvarez RL Whetten 《Science (New York, N.Y.)》1998,280(5372):2098-2101
A transition from metal-like double-layer capacitive charging to redox-like charging was observed in electrochemical ensemble Coulomb staircase experiments on solutions of gold nanoparticles of varied core size. The monodisperse gold nanoparticles are stabilized by short-chain alkanethiolate monolayers and have 8 to 38 kilodaltons core mass (1.1 to 1.9 nanometers in diameter). Larger cores display Coulomb staircase responses consistent with double-layer charging of metal-electrolyte interfaces, whereas smaller core nanoparticles exhibit redox chemical character, including a large central gap. The change in behavior is consistent with new near-infrared spectroscopic data showing an emerging gap between the highest occupied and lowest unoccupied orbitals of 0.4 to 0.9 electron volt. 相似文献
84.
F. Caravaca M. M. Alguacil M. Vassileva G. Díaz A. Roldn 《Land Degradation \u0026amp; Development》2004,15(2):153-161
A field experiment was carried out to compare the effectiveness of inoculation with three arbuscular mycorrhizal (AM) fungi, namely Glomus intraradices Schenck & Smith, Glomus deserticola (Trappe, Bloss. & Menge) and Glomus mosseae (Nicol & Gerd.) Gerd. & Trappe, and the addition of Aspergillus niger‐treated dry olive cake (DOC) in the presence of rock phosphate, in increasing root nitrate reductase (NR) and acid phosphatase activities, mycorrhizal colonization, plant growth and nutrient uptake in Dorycnium pentaphyllum L. seedlings afforested in a semiarid degraded soil. Three months after planting, both the addition of fermented DOC and the mycorrhizal inoculation treatments had increased root NR activity significantly, particularly the inoculation with G. deserticola (by 75 per cent with respect to non‐inoculated plants), but they had no effect on root acid phosphatase. Mycorrhizal inoculation treatments with G. deserticola or G. mosseae on their own were even more effective than the addition of fermented DOC alone in improving the growth and (NPK) foliar nutrients of D. pentaphyllum plants. The combined treatment involving the application of microbially‐treated agrowastes and mycorrhizal inoculation with AM fungi, particularly with G. mosseae, can be proposed as a successful revegetation strategy for D. pentaphyllum in P‐deficient soils under semiarid Mediterranean conditions. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献
85.
M.M. Alguacil E. Torrecillas D.A. Fernández A. Roldán 《Soil biology & biochemistry》2011,43(7):1498-1508
A mesocosm experiment was conducted to investigate whether communities of arbuscular mycorrhizal (AM) fungi associated with roots of native (Piptatherum miliaceum, Retama sphaerocarpa, Psoralea bituminosa, Coronilla juncea, and Anthyllis cytisoides) and for comparison (Lolium perenne) seedlings in a heavy-metal-contaminated, semiarid soil were affected by the application of composted sugar beet waste. We also investigated whether there were relation between AMF diversity and metal concentration (Al, Cd, Cu, Fe, Mn, Pb and Zn) and total P in shoot as well as some soil parameters (total organic carbon and total N) when the SB waste was added to the soil. We analyzed a portion of approximately 795 base pairs of the small-subunit (SSU) rRNA gene by nested PCR, cloning, sequencing, and phylogenetic analyses. Twelve different AMF sequence types were distinguished: seven of these belonged to Glomus group A, one to Glomus group B, one to Diversispora, one to Archaeospora, and two to Paraglomus. The AM fungal populations colonizing roots in a heavy-metal-polluted soil were quite dependent on the host plant, the highest diversity values being obtained in authochtonous plants recognized as metallophytes, such as P. bituminosa, and in an allochtonous, invasive species (L. perenne). No significant correlation was found between AMF diversity and plant metal concentration and soil parameters. Excepting P. bituminosa, when sugar beet waste was added to soil, the populations of AM fungi in roots increased and the shoot metal concentrations decreased in all host plant species studied. Therefore, the addition of sugar beet waste can be considered a good strategy for the remediation and/or phytostabilization of mine tailing sites. 相似文献
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It is suggested that the diversity of arbuscular mycorrhizal fungi (AMF) and their association with distinct plants species are crucial in the early stages of revegetation procedures since the AMF roots colonisation plays an important role improving plant establishment and growth. We carried out a study where we analyse the AMF community composition in the roots of Ephedra fragilis, Rhamnus lycioides, Pistacia lentiscus and Retama sphaerocarpa fourteen months after revegetation in a Mediterranean semiarid degraded area of southeast Spain in order to verify whether different plant species can variably promote the diversity of AM fungi in their rhizospheres after planted. We analysed a portion of approximately 795 bases pairs of the small-subunit ribosomal DNA by means of nested PCR, cloning, sequencing and phylogenetic analyses. Eight fungal sequence types belonging to Glomus group A and B and to the genus Paraglomus were identified. The different plant species had different AM fungal community composition. Thus, R. lycioides harboured the highest number of four fungal sequence types while from E. fragilis only two types could be characterized that were specific for this plant species. P. lentiscus and R. sphaerocarpa harboured each one three sequence types and two of them were shared. All AMF sequence types were found in the natural soil. These results show that one effective way of restoring degraded lands is to increase the number of plant species used, which would increase the AMF diversity in the soil and thus the below-ground, positive interactions. 相似文献
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This study examined the effect of acute bull exposure around the time of artificial insemination (AI) on oxytocin and progesterone concentrations, and pregnancy rates in dairy cows. Ninety six dairy cows, stratified according to parity into primiparous and pluriparous, were divided into three groups; short bull exposure (SBE; 10 min, n = 32), long bull exposure (LBE; 4 h, n = 32) or no bull exposure (NBE; n = 32). On day 45 post‐partum, all cows were treated with PGF2α on three occasions 11–14 days apart to synchronize oestrus. They were submitted to fixed time AI 80 h after the third PGF2α injection. Cows in the SBE and LBE groups were artificially inseminated 5 min after the introduction of the bull. From a subset of cows (n = 6 per group; three primiparous and three pluriparous), blood samples were collected once every 5 min starting 15 min before AI until 15 min after AI and analysed for oxytocin concentrations. Additional blood samples were collected for measurements of progesterone (P4) concentrations once daily for 4 days starting on the day of AI and once every 3 days thereafter until day 22. The effects of bull exposure, time, parity, difficulty of AI, and pregnancy on oxytocin and P4 concentrations were analysed using the mixed linear model procedure. Mean oxytocin concentrations or change in oxytocin concentrations after bull exposure or AI were not different among groups. Pregnancy rates for the NBE, SBE and LBE groups were 55.5%, 33.3% and 44.4%, respectively, and were not different among groups. In conclusion, acute bull exposure around the time of AI did not affect oxytocin and progesterone concentrations and did not improve pregnancy rates in dairy cattle under these farms conditions. 相似文献
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LJ O'Donnell BR Sheerin JM Hendry MJ Thatcher WW Thatcher MM LeBlanc 《Reproduction in domestic animals》2003,38(3):233-235
The mare exhibits nocturnal uterine contractions in the last 6 days of gestation. It is hypothesized that estradiol 17β (O17β) may be associated with the nightly increase in uterine contractions. The 24‐h secretion pattern of plasma O17β was measured in 3 pony mares in late gestation to identify changes in release as the mare neared parturition. Blood was collected weekly at 08:00 hours beginning on day 240 and every third day from day 330 until delivery. Serial blood samples were collected from each mare every 30‐min for 24‐h beginning on gestation day 310 and every sixth day thereafter until parturition. Concentrations of O17β were elevated at night with lowest concentrations occurring directly before sunset (p < 0.01). The natural log of the variance was increased at sunset (p < 0.01) and was decreased during the 6‐h period immediately after sunrise. This pattern was especially evident in the 6 days that preceded parturition. The contrast between nocturnal and daytime concentrations of O17β in the last 6 days of gestation may contribute to night‐time delivery in the mare. 相似文献
89.
Soil sustainability indicators following conservation tillage practices under subtropical maize and bean crops 总被引：2，自引：1，他引：1
Conservation management systems such as no tillage may enhance sequestration of soil C. The soil properties that contribute to soil C storage under such systems are still largely unknown, especially in subtropical agroecosystems. We investigated the influence of tillage [mouldboard plough (MP) and no tillage (NT)] on soil organic C, microbial biomass and activity, structural stability and mycorrhizal status of a field cultivated with maize (Zea mays L.) or bean (Phaseolus vulgaris L.) on a Vertisol in Northern Tamaulipas, Mexico. Crop type, tillage system and soil depth had a significant effect on soil organic C, aggregate stability and bulk density. Soil organic C, microbial biomass C and N and dehydrogenase and phosphatase activities were greater with NT than with MP, particularly under bean cultivation. In the 0–5 cm layer, microbial biomass C and N were, on average, about 87 and 51% greater in the soils cultivated with bean and maize, respectively, under NT than under MP. Higher levels of mycorrhizal propagules, glomalin related soil protein (GRSP) and stable aggregates were produced under NT than under MP in both crops. The no-tillage system can be considered an effective management practice for carrying out sustainable agriculture under subtropical conditions, due to its improvement of soil physical and biochemical quality and soil C sequestration. 相似文献
90.
The finding of Alzheimer type II astrocytes, in addition to the pathognomonic combination of laminar cerebrocortical necrosis and eosinophil infiltration, in the brains of pigs is reported for the first time in cases of indirect salt poisoning following water deprivation. 相似文献