赤水乌骨鸡TYR基因多态性及生物信息学分析     

祖盘玉  李维  林家栋  李洪林  刘洋  牟腾慧  龙广丽  简华峰  张福平 《南方农业学报》2019,50(12):2806-2811

[目的]找出赤水乌骨鸡和泰和乌鸡TYR基因外显子的多态位点(SNP),为后期利用分子遗传学技术选育乌度更深的赤水乌骨鸡提供参考依据.[方法]采用DNA混池结合PCR产物直接测序法,分析赤水乌骨鸡和泰和乌鸡TYR基因外显子的SNP位点,并对筛选出的SNP位点进行生物信息学分析.[结果]在泰和乌鸡中发现4个SNPs位点,分别为Exonl-C829T、Exon1-C921T、Intron 1-A 1018G和Intron2-T79A,其中,Exon1-C829T和Exon1-C921T为同义突变;在赤水乌骨鸡中发现5个SNPs位点,分别为Intron1-G156A、Intron2-T7C、Intron4-G201A、Intron4-C221T和Exon5-A205G(非编码区).利用在线生物信息学分析软件对TYR基因突变前后编码区序列的mRNA二级结构进行预测分析,结果发现赤水乌骨鸡的mRNA二级结构未发生变化,而泰和乌鸡Exon1-C829T和Exon1-C921T的突变均引起mRNA二级结构改变,使得TYR基因mRNA二级结构最小自由能减小,即二级结构稳定性增加.赤水乌骨鸡TYR蛋白二级结构由α-螺旋、无规则卷曲、β-转角及扩展链组成,分别占26.65%、55.31%、3.41%和14.63%.[结论]Exon1-C921T突变可能会对鸡的肉色产生影响,故推测Exon1-C921T是影响赤水乌骨鸡和泰和乌鸡乌度差异的原因之一.  相似文献   

基于近红外漫反射光谱的规模化奶牛场粪水氮磷定量分析及模型构建   总被引：2，自引：2，他引：2  

赵润  牟美睿  王鹏  孙迪  刘海学  张克强  杨仁杰 《农业环境科学学报》2019,38(8):1768-1776

为建立规模化奶牛场粪水中氮磷含量现场快速检测方法,以实现准确预测的同时替代常规监测程序,选取23家天津市典型种养结合模式的规模化奶牛场,围绕粪水处理全过程环节依次开展样品采集、实验室常规化学检测、近红外漫反射光谱采集,并进行主成分分析和偏最小二乘分析,建立多种动态复合影响因素条件下的全局、全程快速检测定量分析模型。结果表明:主成分分析不仅反映出同一奶牛场粪水有机组分随处理环节的变化,而且也反映出不同奶牛场粪水样品的差异性,以及在粪水处理过程中各因素对后续模型的影响程度。建立的全过程环节定量分析模型对总氮含量预测结果与实际含量的线性拟合相关系数R为0.96,预测均方根误差RMSEP为187.80;对总磷含量预测结果与实际含量的线性拟合相关系数R为0.91,预测均方根误差RMSEP为3.59。建立的全局定量分析模型对总氮含量预测结果与实际含量的线性拟合相关系数R为0.96,预测均方根误差RMSEP为238.59;总磷含量预测结果与实际含量的线性拟合相关系数R为0.91,预测均方根误差RMSEP为6.56。研究表明,基于近红外漫反射光谱和偏最小二乘法对规模化奶牛场粪水处理全过程环节粪水样品中氮、磷含量进行定量分析是可行的;纵向模型比横向模型能提供更好的预测结果;近红外漫反射光谱技术可实时、快速、高效地对规模化奶牛场粪水处理全过程总氮和总磷进行跟踪和监控。  相似文献   

不同栽植密度对辣椒产量的影响研究     

肖莉  郑元红  牟东岭  毛诗友  马西艳  胡显钊  王慧 《现代农业科技》2011,(2):119-121

为进一步提高毕节地区名优辣椒的种植水平,开展不同栽植密度对金椒1号和黔西泡通椒产量影响的研究试验,结果表明:金椒1号、黔西泡通椒栽植密度分别为8 596、8 748株/666.67 m2时,最高产量分别可达225.55、248.17 kg/667.67 m2,经济效益最好。  相似文献   

花溪灰萝卜区域试验研究   总被引：2，自引：0，他引：2  

牟琼  王应芬  杨义成  吴佳海  周玉锋  吴有松  宋世伦 《草业与畜牧》2013,(2)

为筛选出具有推广潜力、产量较高、抗逆性强并适应贵州气候条件的饲用灰萝卜新品种,对3个灰萝卜品种的生育期、生产性能、抗逆性等进行了对比分析,结果表明,花溪灰萝卜品种优势突出,表现为产量高、抗逆性强,具有较大的推广潜力.  相似文献   

TGF β₁ inhibits the maturation of dendritic cells and down-regulates TLR4 expression     

MOU Hai-bo  LIN Mao-fang  CEN Hong  HUANG He 《园艺学报》2005,21(8):1476-1479

AIM: To investigate the effects of transforming growth factor β1 (TGF-β1) on murine-derived dendritic cells (DC). METHODS: Murine bone marrow cells were cultured with GM-CSF and TGF-β1 to develop TGF β-DC. Then they were stimulated by lipopolysaccharide (LPS). Their phenotypes were assessed by flow cytometry (FCM). The allogeneic stimulating capacity of DC was measured by mixed lymphocyte reaction (MLR) using BrdU ELISA method. IL-12 p70 protein was detected by ELISA and the expressions of Toll like receptor 4 (TLR4) on DCs were measured by semi-quantitative RT-PCR and FCM. RESULTS: Compared to immature DC (imDC) cultured with GM-CSF alone, the expressions of CD80, CD86, I-Ab and CD40 in TGF β-DC were lower. The TGF β-DC was resistant to maturation by LPS. Maturation resistance was evident from a failure to up-regulate CMs, to stimulate larger T cell proliferation and to increase secretion of IL-12 p70. Down-regulation of TLR4 expression on TGF β-DC was also found. CONCLUSION: TGF-β1 inhibits the expression of co-stimulatory molecules on DC. It is resistant to maturation stimulus (LPS) and might be linked with TLR4 down-regulation.  相似文献   

Comparative phosphoproteome analysis of cardiomyocytes preconditioned by diazoxide     

LI Hong  XIAO Ying-bin  YANG Tian-de  MOU Zhi-rong  ZOU Li-yun  HUANG He 《园艺学报》2007,23(5):844-847

AIM: To analyze and identify the phosphoproteins associated with diazoxide preconditioning. METHODS: Proteomics technique was used to investigate the changes of phosphoprotein after diazoxide preconditioning. Adult rat ventricular myocytes were pretreated in the presence and absence of 200 μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched respectively from control and diazoxide pretreated groups were then separated by two-dimensional (2D) gel electrophoresis and stained with sliver staining kit. Phosphoproteins of interest were further identified by mass spectrometry. RESULTS: Associated with diazoxide preconditioning, the proteins of chaperonin containing TCP-1 and hypothetical protein XP_346548 were phosphorylated significantly. The proteins of 94 kD glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphorylated markedly (P<0.05). CONCLUSION: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in response to diazoxide preconditioned signaling, which may mediate myocardioprotection signaling downstream mitochondrial staining dish K_ATP channel induced by ischemic preconditioning.  相似文献   

Increased expression and possible roles of nicotinamide N-methyltransferase in pancreatic islets of STZ-induced diabetic monkeys     

MOU Bo  YANG Zhi-hong  ZHAO Jia-wei  WANG Xuan-chun  DONG Xue-hong  LIU Yu  HU Ren-ming 《园艺学报》2007,23(5):888-892

AIM: To verify and localize the expression of nicotinamide N-methyltransferase (NNMT) in pancreas of streptozotocin(STZ)-induced diabetic monkeys and understand its important role in β-cell destruction in the pathogenesis of diabetes. METHODS: Through an olig-microarray gene chip, NNMT was identified as the most obviously up-regulated genes in pancreas of STZ-induced diabetic monkeys versus controls. Semiquantitative RT-PCR and Western blotting were performed to verify the differential expression at mRNA and protein level respectively. Then the cellular localization of NNMT expression within pancreas was identified by immunohistochemical(IHC) staining.RESULTS: An obvious high expression of NNMT at both mRNA and protein levels was shown in pancreas of STZ-induced diabetic monkeys compared to that of controls. Further localization of the protein by IHC staining in pancreas specimens showed that its altered expression was restricted to central islets, most of which were β cells.CONCLUSION: Expression of NNMT is increased in islets of STZ- induced diabetic monkeys, which infers that NNMT might participate in the process of β cell damage in diabetes probably through the mechanism of energy metabolism disturbance.  相似文献   

双向电泳结合蛋白质印迹技术分析猪囊尾蚴乳酸脱氢酶     

方文  包怀恩  肖靓靓  牟荣 《黑龙江畜牧兽医》2011,(15)

为了研究猪囊尾蚴乳酸脱氢酶(LDH)的表达,试验选择四川省雅江县呷拉乡猪带绦虫病患者,给其口服槟榔-南瓜子驱虫,收集、制备虫卵悬液(8万个/mL),再给3头20日龄三元杂交乳猪猪灌胃虫卵悬液,每头1 mL,40 d后收集、制备猪囊尾蚴蛋白,进行双向电泳(2-DE)分析,将凝胶蛋白斑点转移至聚偏氟乙烯膜(PVDF膜),用自制的大鼠抗猪带绦虫LDH血清作为一抗、健康大鼠血清作为阴性对照进行蛋白质印迹(Western-blotting)分析。结果表明:双向电泳凝胶共检测到(207±9)个蛋白质斑点,相对分子质量(Mr)为14 400～94 000,等电点(pI)为3.0～10.0;试验组特异性抗原抗体阳性杂交斑点为1个,阴性对照未见阳性杂交斑点;将Western-blotting检测的抗原抗体阳性杂交斑点与原双向电泳凝胶斑点进行比对,找到对应蛋白斑点,经ImageMaster 2D Platinum 5.0软件分析后初步确定该蛋白斑点的pI/Mr为7.03/35 368,与猪带绦虫LDH的pI/Mr理论推导值接近,说明猪囊尾蚴表达LDH。  相似文献   

以生态恢复为导向的郪江流域大英段植物景观规划     

冯美文  牟江 《林业调查规划》2014,(2):92-97

流域植被作为基础的自然"本底",在构建和维护河流生态系统稳定方面有着重要作用。通过对生态恢复定义及模式的探析,以流域植物为主要分析对象,浅析其界定与特征。以郪江流域大英段生态恢复项目为载体,展开流域植物景观规划研究,阐述总体策略引导下的植物评价及规划。  相似文献   

不同播期对威芋5号产量及生长发育的影响   总被引：1，自引：0，他引：1  

李杨 《长江蔬菜》2014,(4):31-32

以马铃薯威芋5号为试验材料,研究在威宁县不同播期对威芋5号生理指标和产量的影响。试验结果表明,3月23日播种,威芋5号产量最高,可达2 116.46 kg/667 m2,纯收入为1 134.81元/667 m2,经济效益显著,而不同播期对威芋5号生育期影响不大。  相似文献