Testosterone Influences Water Transport in Porcine Granulosa Cells     

M Grzesiak  L Williams  MR Luck 《Reproduction in domestic animals》2013,48(4):e52-e54

The development of antral ovarian follicles entails fluid accumulation, but the mechanisms regulating water flux are unknown. Aquaporins are small, integral membrane proteins facilitating passive movement of water, some of which are known to be regulated by steroid hormones. The aim of this study was to determine whether testosterone (T) influences water transport in porcine granulosa cells. To assess water movement, the swelling of granulosa cells when moved from isotonic (319 mOsm) to hypotonic (95 mOsm) medium was measured after 12‐hour pre‐incubation in the presence of either testosterone (T), the antiandrogen 2‐hydroxyflutamide (HF) or HF and T together. Pre‐incubation with T increased the swelling of granulosa cells (p < 0.01) and this was abolished by HF (p < 0.001). Neither T nor HF affected cells in isotonic medium (319 mOsm). The results indicate that T acting via intracellular androgen receptors increases water permeability of porcine granulosa cells, probably through the regulation of aquaporin activity.  相似文献   

Abomasal parasite syndrome in North American elk (Cervus elaphus canadensis)     

MR Woodbury  NMA Parry 《New Zealand veterinary journal》2013,61(4):235-240

CASE HISTORY An 18-month clinical course of chronic ill-thrift, weight loss and emaciation, and eventual death occurred in a group of 520 translocated elk of mixed age and sex. Trans-location was carried out without regard to animal welfare or health risks associated with the translocation. Mortality was approximately 84% (436/520) despite supportive nutritional and medical treatment.

PATHOLOGICAL FINDINGS: General clinical and post-mortem examinations indicated only Se and Cu imbalances and nutritional inadequacy. Additional purposeful post-mortem examination and histological evaluation of tissue sections from four of the affected adult elk demonstrated elevated abomasal pH and proliferative abomasal lesions as the most significant findings, consistent with Type-II ostertagiosis; intra-lesional nematodes were seen in the abomasum of two animals.

DIAGNOSIS: Fading elk syndrome, or abomasal parasite syndrome in elk.

CLINICAL RELEVANCE: Abomasal parasite syndrome initiated by Type-II ostertagiosis should be considered as a differential diagnosis in cases of ill-thrift and wasting in elk or elk-red deer hybrids. Changes to the architecture and secretory function of the abomasal wall lead to apparently irreversible digestive pathophysiology and nutritional disease.  相似文献   

Genetic diversity of a newly established population of golden eagles on the Channel Islands,California     

Sarah A. Sonsthagen  Timothy J. Coonan  Brian C. Latta  George K. Sage  Sandra L. Talbot 《Biological conservation》2012,146(1):116-122

Gene flow can have profound effects on the genetic diversity of a founding population depending on the number and relationship among colonizers and the duration of the colonization event. Here we used data from nuclear microsatellite and mitochondrial DNA control region loci to assess genetic diversity in golden eagles of the recently colonized Channel Islands, California. Genetic diversity in the Channel Island population was low, similar to signatures observed for other recent colonizing island populations. Differences in levels of genetic diversity and structure observed between mainland California and the islands suggests that few individuals were involved in the initial founding event, and may have comprised a family group. The spatial genetic structure observed between Channel Island and mainland California golden eagle populations across marker types, and genetic signature of population decline observed for the Channel Island population, suggest a single or relatively quick colonization event. Polarity in gene flow estimates based on mtDNA confirm an initial colonization of the Channel Islands by mainland golden eagles, but estimates from microsatellite data suggest that golden eagles on the islands were dispersing more recently to the mainland, possibly after reaching the carrying capacity of the island system. These results illustrate the strength of founding events on the genetic diversity of a population, and confirm that changes to genetic diversity can occur within just a few generations.  相似文献   

An operational UAV-based approach for stand-level assessment of soil disturbance after forest harvesting     

Bruce Talbot  Johannes Rahlf  Rasmus Astrup 《Scandinavian Journal of Forest Research》2018,33(4):387-396

The effectiveness of generating virtual transects on unmanned aerial vehicle-derived orthomosaics was evaluated in estimating the extent of soil disturbance by severity class. Combinations of 4 transect lengths (5–50 m) and five sampling intensities (1–20 transects per ha) were used in assessing traffic intensity and the severity of soil disturbance on six post-harvest, cut-to-length (CTL) clearfell sites. In total, 15% of the 33 ha studied showed some trace of vehicle traffic. Of this, 63% of was categorized as light (no visible surface disturbance). Traffic intensity varied from 787 to 1256 m ha^?1, with a weighted mean of 956 m ha^?1, approximately twice the geometrical minimum achievable with CTL technology under perfect conditions. An overall weighted mean of 4.7% of the total site area was compromised by severe rutting. A high sampling intensity, increasing with decreasing incidence of soil disturbance, is required if mean estimation error is to be kept below 20%. The paper presents a methodology that can be generally applied in forest management or in similar land-use evaluations.  相似文献   

Evaluation of a point‐of‐care electrochemical meter to detect subclinical ketosis and hypoglycaemia in lactating dairy cows          下载免费PDF全文

A Zakian  M Tehrani‐Sharif  MR Mokhber‐Dezfouli  M Nouri  PD Constable 《Australian veterinary journal》2017,95(4):123-128

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Information Technologies and Ecological Site Descriptions     

Curtis J. Talbot  Steven B. Campbell  Michael Hansen  Alan B. Price 《Herpetological Monographs》2010,32(6):55-59

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Characterisation of adult green lacewing (Chrysoperla carnea) digestive physiology: impact of a cysteine protease inhibitor and a synthetic pyrethroid     

Evan A Mulligan  Natalie Ferry  Lise Jouanin  Jörg Romeis  Angharad MR Gatehouse 《Pest management science》2010,66(3):325-336

BACKGROUND: In spite of concern regarding potential non‐target effects of GM crops, few studies have compared GM pest control with conventional methods. The impacts of cypermethrin and oilseed rape expressing oryzacystatin‐1 (OC‐1) were compared in this study on the predator Chrysoperla carnea (Stephens). RESULTS: Adults fed purified rOC‐1 showed a subtle shift in digestive protease profile, with an increasing reliance on serine proteases (chymotrypsin), increase in aspartic proteases and a slight reduction in elastase activity. Although there were no effects on mortality, onset of oviposition was delayed; however, once egg production commenced, egg laying and hatching success rates were comparable with those of controls. Oryzacystatin‐1 expressed in pollen showed no detrimental effects. Cypermethrin had no effect on mortality owing to high levels of non‐specific esterase activity resulting in partial breakdown of the insecticide. In spite of this, there was a significant delay in onset of oviposition and a significant reduction in egg production and viability. CONCLUSION: This study demonstrates the potential for pest management to impact on predators, but importantly it highlights the ability of the predator to detoxify/respond to treatments with different modes of action. In this case, exposure to an insecticide carried a greater fitness cost than exposure to a protease inhibitor expressed in transgenic crops. Copyright © 2009 Society of Chemical Industry  相似文献   

Bosonic stimulation in the formation of a bose-einstein condensate     

H Miesner  DM Stamper-Kurn  MR Andrews  DS Durfee  S Inouye  W Ketterle 《Science (New York, N.Y.)》1998,279(5353):1005-1007

The formation of a Bose-Einstein condensate of a dilute atomic gas has been studied in situ with a nondestructive, time-resolved imaging technique. Sodium atoms were evaporatively cooled close to the onset of Bose-Einstein condensation and then suddenly quenched to below the transition temperature. The subsequent equilibration and condensate formation showed a slow onset distinctly different from simple relaxation. This behavior provided evidence for the process of bosonic stimulation, or coherent matter-wave amplification, crucial to the concept of an atom laser.  相似文献   

Autophagic fungal cell death is necessary for infection by the rice blast fungus   总被引：2，自引：0，他引：2  

Veneault-Fourrey C  Barooah M  Egan M  Wakley G  Talbot NJ 《Science (New York, N.Y.)》2006,312(5773):580-583

Rice blast is caused by the fungus Magnaporthe grisea, which elaborates specialized infection cells called appressoria to penetrate the tough outer cuticle of the rice plant Oryza sativa. We found that the formation of an appressorium required, sequentially, the completion of mitosis, nuclear migration, and death of the conidium (fungal spore) from which the infection originated. Genetic intervention during mitosis prevented both appressorium development and conidium death. Impairment of autophagy, by the targeted mutation of the MgATG8 gene, arrested conidial cell death but rendered the fungus nonpathogenic. Thus, the initiation of rice blast requires autophagic cell death of the conidium.  相似文献   

The effect of feeding on the secretion of pepsin,trypsin and chymotrypsin in the Atlantic salmon,Salmo salar L     

Sigfús Einarsson  Dr. P. Spencer Davies  Clive Talbot 《Fish physiology and biochemistry》1996,15(5):439-446

Pepsin stored in the stomach mucosa of the Atlantic salmon (Salmo salar L.) increases within two days of the onset of starvation. Trypsin and chymotrypsin in the pyloric caeca/pancreas behave similarly, indicating that when no food is present in the gut, digestive enzymes accumulate in the secretory tissues. As a corollary, trypsin and chymotrypsin activities in the gut contents fell during starvation, indicating that secretion is greatly reduced when food is not present. At the onset of feeding, pepsin is rapidly synthesised in the mucosal tissues and then secreted. Twenty four hours after feeding, the pepsin levels of the mucosa are still low, suggesting that synthesis may be a rapid response to the presence of food in the stomach. Secretion of trypsin and chymotrypsin appears to take place as soon as digesta enters the intestine, between 4 and 14h after feeding, and resynthesis of enzyme precursors appears to be complete again within a further 11h. It is suggested that both synthesis and release may be under the control of cholecystokinin.  相似文献