Identification,occurrence and clinical findings of canine hemoplasmas in southern Brazil     

Stella de Faria Valle  Joanne B. Messick  Andrea Pires dos Santos  Luiz Carlos Kreutz  Naila Cristina Blatt Duda  Gustavo Machado  Luis Gustavo Corbellini  Alexander Welker Biondo  Felix Hilario Diaz González 《Comparative immunology, microbiology and infectious diseases》2014

Hemoplasmas are ubiquitous pleomorphic and epicellular bacteria detected in erythrocytes in several species. In Brazil, studies on hemoplasmas have not included information on occurrence, clinical signs, and risk factors in dogs. This paper investigates the occurrence of hemoplasmas in dogs, focusing on risk factors and clinical status. Conventional PCR for the four types of canine hemoplasmas was performed in 331 blood samples collected from dogs clinically treated at a teaching veterinary hospital. Of all samples, 17/331 (5.1%) were positive for Mycoplasma haemocanis and 6/331 (1.8%) were positive for a ‘Candidatus Mycoplasma haemominutum-like’ organism. Risk factors included the presence of vectors, old age, dog bite wounds, and neoplastic diseases. In the multivariate analysis, a 4.40 odds ratio in dogs with vector-borne diseases indicated risk for hemoplasmosis. There was correlation between hemoplasma infection and neoplastic disease, suggesting that neoplastic conditions are a risk factor for hemoplasma infection in dogs.  相似文献   

Relevance of synovial fluid chondroitin sulphate as a biomarker to monitor polo pony joints     

Raquel Y.A. Baccarin  Luciane Rasera  Thaís S.L. Machado  Yara M. Michelacci 《Canadian journal of veterinary research》2014,78(1):50-60

Osteoarthritis (OA) of the metacarpophalangeal joint is the most common articular disease in polo ponies leading to early retirement. A biomarker that would discriminate between pathological and physiological changes secondary to exercise could be helpful in OA prevention. The aim of this study was to investigate the effects of polo training on synovial fluid biomarkers of inflammation and cartilage turnover in polo ponies of different skill levels. Synovial fluid samples were collected from metacarpophalangeal joints of polo ponies before and during the polo season (320 d). Nucleated cells, soluble protein, prostaglandin E₂ (PGE₂), glycosaminoglycans (GAG), and urea were measured. The main synovial fluid GAG are chondroitin sulphate (CS, ~25 μg/mL) and hyaluronic acid (HA, ~400 μg/mL). After a polo match, a transitory increase in protein and PGE₂, but not CS and HA, occurred (expressed as urea ratio), returning to basal levels in 24 h. During the polo season, the number of synovial fluid nucleated cells was always in the normal range. Increases in protein and HA occurred during the initial 40 to 80 d, returning to basal levels afterwards. In contrast, in polo prospects the concentration of CS steadily increased during the season. Long-term follow-up revealed that the synovial fluid CS was significantly higher in polo ponies that developed joint diseases within 24 months following our study. In conclusion, CS seems to be an early marker of articular cartilage damage.  相似文献   

Hereditary renal disease in a mutant strain of rats   总被引：4，自引：0，他引：4  

B B Lozzio  A I Chernoff  E R Machado  C B Lozzio 《Science (New York, N.Y.)》1967,156(783):1742-1744

Disease of the kidney developed in breeding stock of Gunn rats. The renal lesion is the result of a new mutation. The genetic defect is inherited as an autosomal dominant trait and is apparently lethal in the homozygous condition. The abnormality manifests itself as a congenital hydronephrosis with related cystic changes in the kidney.  相似文献   

Assessment of qualitative and quantitative parameters of healthy thyroid gland in adult horses by magnetic resonance imaging     

Gustavo Fernandes Viana  Monica Midon  Mariana Werneck Fonseca  Alessandre Hataka  Luciana Carandina  Laís Melício Cintra Bueno  José Nicolau Próspero Puoli Filho  Vânia Maria de Vasconcelos Machado 《Anatomia, histologia, embryologia》2021,50(5):839-848

In humans and pets, magnetic resonance imaging (MRI) provides important adjunctive anatomic information about the thyroid and its adjacent structures. To the best of our knowledge, however, no studies have been reported regarding the use of MRI for thyroid evaluation in horses. The aim of this study was to obtain qualitative and quantitative parameters of healthy thyroid gland in adult horses by MRI. Eleven horses were submitted to thyroid MRI evaluation. The healthy thyroid of these animals was ensured by normal examinations performed before (free thyroxine and ultrasound) and after (cytology) to MRI. It was concluded that the dorsal spin-echo T1-weighted, fast spin-echo T2-weighted, sagittal STIR and a three-dimensional T1-weighted gradient echo were the sequences that best characterized the thyroid lobes. Qualitatively, there was a significant variation in the elliptical format of some lobes considering the sagittal plane, which oscillated between rounded and flattened conformation. Excellent reproducibility of the quantitative thyroid parameters obtained was verified. The thyroid gland total volume mean of observers was 14.31 cm³ (±0.69). In relation with comparisons between quantitative parameters of left and right lobes, only differences regarding their respective lengths were observed. Thus, we can infer that the difference in some thyroid lobes format can be explained by the significant variation identified between left and right lobes lengths.  相似文献   

Influence of different warming temperatures on the vitrified testicular fragments from pre-pubertal cat     

Juliana de Souza Fernandes  Beatriz Evaristo de Almeida Tabosa  Bruna Farias Brito  Herlon Victor Rodrigues Silva  David Baruc Cruvinel Lima  Lúcia Daniel Machado da Silva 《Reproduction in domestic animals》2021,56(10):1342-1348

Testicular vitrification is an alternative to preserve the genetic material of pre-pubertal animals. However, there are few studies on post-vitrification warming. Hence, the aim was to compare the influence of different warming temperatures on vitrified testicular fragments from pre-pubertal cats. The testicles were fragmented and divided into a control group (non-vitrified) and vitrified, using an association between dimethylsulphoxide and glycerol. The vitrified fragments were warmed at 50, 55 and 60°C/5 s. Morphological and morphometric evaluations were carried out using classical histology. Afterwards, the mitochondrial activity was evaluated using Rhodamine 123. The data were expressed in mean and standard error. The differences were considered significant when p < .05. In the histomorphological analysis, the testicular fragment presented seminiferous tubules with poorly developed germinal epithelium, compatible with pre-pubertal animals. The group warmed at 50°C presented similar to the control regarding the maintenance of the integrity of the tubules and cells, without stromal rupture and lamina propria alteration, as well as regarding the maintenance of the junctions between the cells. The group warmed at 55°C showed reduction of the cell junctions, and the one warmed at 60°C had increased detachment of the basement membrane (p < .05). The warming caused a reduction in the tubular diameter inversely proportional and progressive to the increase in temperature, with the highest diameter in the control group and the lowest in the 60°C group. The control group showed a lower incidence of Rhodamine 123, followed in ascending order of the warmings at 55 and 60°C. The higher mitochondrial activity was obtained with 50°C, showing an increase of the metabolic cell function at this temperature. It was concluded that the testicular fragment of pre-pubertal cats presents a better preserved morphology, morphometry and viability when warmed at 50°C.  相似文献   

The transfer of triazine resistance from Brassica napus L. to B. oleracea L. I. Production of F1 hybrids through embryo rescue     

R. Ayotte  P. M. Harney  V. Souza Machado 《Euphytica》1987,36(2):615-624

Summary Triazine resistant Brassica napus ssp. oleifera and ssp. rapifera were hybridized to cultivars of B. oleracea ssp. italica, ssp. botrytis, ssp. capitata and ssp. fimbriata. The interspecific embryos did not survive in vivo but could be rescued in vitro using a culture medium developed by Monnier (1973). The embryos did not grow directly into normal plants but were successfully regenerated using the protocol developed by Keller (1984). Hybridization efficiency ranged from 0 to 2.64 hybrids per pollination. Interspecific embryo abortion may be related to abnormal endosperm development.  相似文献   

The transfer of triazine resistance from Brassica napus L. to B. oleracea L. II. Morphology,fertility and cytology of the F1 hybrid     

R. Ayotte  P. M. Harney  V. Souza Machado 《Euphytica》1988,37(2):189-197

Summary F₁ hybrids of triazine resistant Brassica napus and triazine susceptible B. oleracea were morphologically intermediate to the parent species. Of 49 hybrids examined, 44 had 28 chromosomes, two had 37, one had 38 and two had 56. The 38-chromosome plant was thought to be a matromorph, the others, A₁C₁C (28), A₁C₁CC (37) or A₁A₁C₁C₁CC (56) type hybrids. Pollen stainability averaged 9.0% in the sesquidiploid, 32.0% in the tetraploids and 89.5% in the hexaploids. All the interspecific hybrids were resistant to 1.0×10^-4 mol L^-1 atrazine. The sesquidiploid hybrids produced gametes with chromosome numbers ranging from 9 to 17 and the tetraploid hybrid gametes had chromosome numbers from 15 to 22. Most hybrids produced self-seed. The partial fertility of these hybrids may permit their backcrossing to one or both parents.  相似文献   

Participatory maize breeding for low nitrogen tolerance   总被引：2，自引：0，他引：2  

Altair Toledo Machado  Manlio Silvestre Fernandes 《Euphytica》2001,122(3):567-573

The local maize variety Sol da Manhã has a broad genetic background. It was identified in 1985 in a participatory evaluation trial as being suitable for cultivation under low soil fertility conditions in Sol da Manhã, situated in Seropédica, State of Rio de Janeiro, Brazil. The variety was then improved for 6 selection cycles by the formal breeding sector at the experimental station of Embrapa Agrobiologia. There were three cycles of mass selection, one selection cycle between and within half-sib families, one selection cycle within full-sib families, and one selection cycle between and within S₁ families. Thereafter, the variety was improved in collaboration with the informal sector, viz. a group of farmers of the agricultural community of Sol da Manhã for 6 cycles of mass selection. The variety was evaluated in 1994 in field trials for grain yield and nitrogen use efficiency. Sol da Manhã can be characterised as efficient in nitrogen use, under both favourable and unfavourable growing conditions.  相似文献   

Coelioscopic-assisted prefemoral ovariosalpingectomy in a d`Orbigny slider (Trachemys dorbigni) using a digital otoscope     

Bernardo Nascimento ANTUNES  Diego DA COSTA  Michelli Westphal DE ATAIDE  Allana Valau MOREIRA  Luis Fernando PEDROTTI  Francisco Schulz JÚNIOR  Cassiano Schmitz NHOATO  Renan Alves STADLER  Marco Augusto Machado SILVA  Maurício Veloso BRUN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(12):1802

The purpose of this study was to assess the unilateral prefemoral coelioscopic-assisted approach for ovariosalpingectomy in a d`Orbigny slider (Trachemys dorbigni) using a digital otoscope. Twenty healthy turtles were randomly assigned to one of two groups, for right (GR; n=10) or left (GL; n=10) prefemoral access, for coelioscopic-assisted ovariosalpingectomy. Anesthesia and surgery times, body weight, and ovary/oviduct weight data were recorded. Anesthesia and surgery times did not differ significantly between the groups. Wound closure was the most time-consuming surgical step. Ovary and body weights significantly affected the exposure time of the ipsilateral and contralateral ovaries, respectively. Two intraoperative complications were reported. All the animals recovered uneventfully. The digital otoscope can be safely and effectively used for coelioscopic-assisted single-access, unilateral prefemoral ovariosalpingectomy in d`Orbigny slider.  相似文献   

Ovum pick-up and in vitro maturation in spotted paca (Cuniculus paca—Linnaeus, 1766)     

Felipe Farias Pereira da Câmara Barros  Pedro Paulo Maia Teixeira  Luciana Cristina Padilha-Nakaghi  Ricardo Andres Ramirez Uscategui  Marina Ragagnin Lima  Victor José Correia Santos  Kayan da Cunha Rossy  Luísa Pucci Bueno Borges  Marcia Rita Fernandes Machado  Wilter Ricardo Russiano Vicente 《Reproduction in domestic animals》2020,55(4):442-447

We tested FSHp, eCG and FSHp + eCG to establish ovum pick-up (OPU) and in vitro maturation method in spotted paca. Eight healthy adult females were subjected to each of four treatments to stimulate ovarian follicular growth. All females were subjected to a hormonal protocol using a single dose of 45 mg of injectable progesterone and single intramuscular injection of 0.075 mg d-cloprostenol on day 6. Ovarian stimulation was carried out as follows: in Group TFE (FSHp and eCG), animals were treated with a single dose of 80 mg of FSHp and 200 IU of eCG intramuscularly on day 6 after the application of progesterone; in Group TF (FSHp), they were treated with a single dose of 80 mg of FSHp intramuscularly on day 6 after application of progesterone; in Group treatment eCG, they were treated with 200 IU of eCG intramuscularly on day 6 after application of progesterone; and in Group TC (saline solution), 1 ml of saline solution was administered to control does. The OPU was performed between 22 and 26 hr after gonadotropin treatments. All recovered oocytes were placed into maturation media and incubated for 24 hr. There were no differences among the mean number of observed follicles, aspirated follicles and oocytes recovered per treatment. Oocyte maturation rates did not differ among groups, except, TF and treatment eCG oocytes had greater maturation rates than TC oocytes. In this study, gonadotropin administration failed to superovulate treated does and increase oocyte retrieval efficiency. Despite the feasibility of the procedure, further studies are needed to develop and refine hormonal protocols for oocyte recovery and in vitro maturation in this species.  相似文献