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581.
The red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera, Curculionidae), has been present in Andalusia since 1993 although its major expansion began in 2005. Currently, the implementation of an action protocol against the red palm weevil based on curative and preventive treatments has a high cost. The aim of this work is to establish a minimum frequency between preventive treatments that would reduce costs. Three frequencies of application were evaluated (at 45, 60 and 90 days).The results show that preventive treatments can decrease the reproductive potential of the pest, but do not prevent the infestation of palm trees. Preventive treatments every 60 days effectively reduce costs and increase the survival of the palm trees.  相似文献   
582.
Organic farming is growing rapidly in its potential for producing healthy foods and has been adopted in a wide range of climate and soil types. The perception among consumers is that organically produced crops possess higher nutritional quality. However, there are many factors that can affect the nutritional quality of crops, and few studies have been able to account for these differences.  相似文献   
583.

Background

Lawsonia intracellularis is a common cause of chronic diarrhoea and poor performance in young growing pigs. Diagnosis of this obligate intracellular bacterium is based on the demonstration of the microbe or microbial DNA in tissue specimens or faecal samples, or the demonstration of L. intracellularis-specific antibodies in sera. The aim of the present study was to evaluate a blocking ELISA in the detection of serum antibodies to L. intracellularis, by comparison to the previously widely used immunofluorescent antibody test (IFAT).

Methods

Sera were collected from 176 pigs aged 8-12 weeks originating from 24 herds with or without problems with diarrhoea and poor performance in young growing pigs. Sera were analyzed by the blocking ELISA and by IFAT. Bayesian modelling techniques were used to account for the absence of a gold standard test and the results of the blocking ELISA was modelled against the IFAT test with a "2 dependent tests, 2 populations, no gold standard" model.

Results

At the finally selected cut-off value of percent inhibition (PI) 35, the diagnostic sensitivity of the blocking ELISA was 72% and the diagnostic specificity was 93%. The positive predictive value was 0.82 and the negative predictive value was 0.89, at the observed prevalence of 33.5%.

Conclusion

The sensitivity and specificity as evaluated by Bayesian statistic techniques differed from that previously reported. Properties of diagnostic tests may well vary between countries, laboratories and among populations of animals. In the absence of a true gold standard, the importance of validating new methods by appropriate statistical methods and with respect to the target population must be emphasized.  相似文献   
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586.
Tropical Animal Health and Production - This study investigated the effect of different levels and consumption periods of Aspergillus oryzae meal on performance, carcass characteristics, blood...  相似文献   
587.
588.
Eight dual-flow continuous culture fermenters (1.03 +/- 0.05 L) were used to assess differences in microbial degradation of the soluble CP fraction of canola meal (CMSCP), soybean meal (SBMSCP), and fish meal (FMSCP) using a completely randomized design with two 9-d experimental periods and a solution of tryptone as a control treatment (control). All fermenters received the same basal diet (58% ground corn, 40% canary grass hay, 0.4% vitamin-mineral premix, 1% CaCO(3), 0.6% salt on a DM basis) in 8 equal portions daily. During sampling on the last 3 d of each period, 90-mL doses containing soluble CP were infused into the fermenters 30 min after the beginning of the first and last feedings of the day. The total amount of soluble CP supplied by the infusions of FMSCP, CMSCP, and SBMSCP was 3.2 g/d, representing 24% of the daily dietary CP intake. Infusion of FMSCP resulted in the greatest (P < 0.05) NH(3)-N concentration (4.6 +/- 0.40 mg/dL) compared with the other treatments (0.5 +/- 0.40 mg/dL). Microbial N flow (g/d) from the fermenters was also greatest (P < 0.05) with FMSCP (1.42 +/- 0.062) compared with the other soluble CP fractions (1.08 +/- 0.062). The efficiency of microbial protein synthesis tended to be lowest with the control diet, and the efficiency of N utilization was lowest with FMSCP treatment. These results indicate that N was limiting microbial growth in the control diet, and there was more rumen-available N with the FMSCP diet compared with the other dietary treatments. The extent of degradation of the soluble CP fraction from fish meal, soybean meal, and canola meal was determined to be 99, 30, and 37% of soluble CP, respectively. These results indicate that the soluble CP fraction is not 100% degraded in all feeds and that assuming a high degradation extent of the soluble CP fraction from soybean meal and canola meal may result in an underestimation of the supply of undegradable protein from these protein sources.  相似文献   
589.
The aim of this study was to estimate the genetic parameters and inbreeding effect on the fertility, embryo mortality and hatchability traits in the Zatorska goose covered by the animal genetic resources conservation program. The material for this study contains information about results of hatching of 18 863 eggs from 721 dams and 168 sires, laid between 1998–2015. Genetic parameters were estimated based on the threshold animal model by the use of Restricted Maximum Likelihood and Gibbs sampling. The percentage of fertilized eggs ranged yearly between 37–80%. The percentage of embryo mortality was very low, ranging between 4.63–23.73%. The percentage of the hatched goslings from the total number of analyzed eggs was on average 33.18%, and 53.72% from fertilized eggs. On average based on both methods, the heritability estimates of the fertility, embryo mortality and hatchability reached 0.36, 0.07, 0.24 for males and 0.44, 0.11, 0.32 for females. The genetic trend had increasing tendency for fertility and hatchability and was stable for embryo mortality for both sexes. The obtained result shows that the Zatorska goose can be still maintained in the reserves of the local gene pool according to current rules and use in the local market as a breed with good reproductive potential.  相似文献   
590.
The aim of this study was to induce the cold‐inducible RNA‐binding protein (CIRBP) expression on cumulus–oocyte complexes (COCs) through exposure to a sub‐lethal cold shock and determine the effects of hypothermic temperatures during the in vitro maturation of bovine oocytes. Nuclear maturation, cortical granule redistribution and identification of cold‐inducible RNA‐binding protein (CIRBP) were assessed after 24 hr of in vitro maturation of control (38.5°C) and cold‐stressed oocytes (33.5°C). The presence of CIRBP was assessed by Western blot in COCs or denuded oocytes and their respective cumulus cells. Based on the odds ratio, cold‐stressed oocytes presented higher abnormal cytoplasmic distribution of cortical granules and nuclear maturation than the control group. Although CIRBP was detected in both control and cold‐stressed groups, cold‐stressed COCs had 2.17 times more expression of CIRBP than control COCs. However, when denuded oocytes and cumulus cells were assessed separately, CIRBP only was detected in cumulus cells in both groups. In conclusion, cold shock induced CIRBP expression, but it negatively affected nuclear maturation and cortical granule distribution of bovine oocytes. Moreover, the expression of CIRBP was only identified in cumulus cells but not in oocytes.  相似文献   
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