Development and validation of chloroplast DNA markers to assist Aegilops geniculata and Aegilops neglecta germplasm management     

Patricia Giraldo  Magdalena Ruiz  Marta Rodríguez-Quijano  Elena Benavente 《Genetic Resources and Crop Evolution》2016,63(3):401-407

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Genomic features of Pseudomonas sp. RGM2144 correlates with increased survival of rainbow trout infected by Flavobacterium psychrophilum     

José M. Vidal  Alexis Fonseca  Pamela Ruiz  Daniela Sepúlveda  Carlos Carrasco  Stefano Scilipoti  Javier Barros  Ariel Valenzuela  Ricardo Saavedra  Nathaly Ruiz-Tagle  Homero Urrutia 《Journal of fish diseases》2023,46(1):1-15

This study evaluated the probiotic potential of the biofilm formed by the strain Pseudomonas sp. RGM2144 on rainbow trout survival. When challenged with the fish pathogen Flavobacterium psychrophilum, Pseudomonas sp. RGM2144 increased rainbow trout survival to 92.7 ± 1.2% (control: 35.3 ± 9.5%, p < .0001). The draft genome of Pseudomonas sp. RGM2144 is 6.8 Mbp long, with a completeness 100% and a contamination of 0.4%. The genome contains 6122 protein-coding genes of which 3564 (~60%) have known functions. The genome and phylogeny indicate that Pseudomonas sp. RGM2144 is a new species in the Pseudomonas genus, with few virulence factors, plasmids, and genes associated with antimicrobial resistance, suggesting a non-pathogenic bacterium with protective potential. In addition, the genome encodes for 11 secondary metabolite biosynthetic gene clusters that could be involved in the inhibition of F. psychrophilum. We suggest that Pseudomonas sp. RGM2144 may be applied as a probiotic in salmonid fish farming.  相似文献   

Stratigraphy of near-valley head quaternary deposits and evidence of climate-driven slope-channel processes in southern Brazilian highlands     

Marcelo Accioly Teixeira de Oliveira  Hermann Behling  Luiz Carlos Ruiz Pessenda  Gisele Leite de Lima 《CATENA》2008

During the past 40 years colluvial and alluvial deposits have been used in Brazil as good indicators of regional landscape sensitivity to Quaternary environmental changes. In spite of the low resolution of most of the continental sedimentary record, geomorphology and sedimentology may favor palaeoenvironmental interpretation when supported by independent proxy data. This paper presents results obtained from pedostratigraphic sequences, in near-valley head sites of southern Brazilian highlands, based on geomorphologic, sedimentologic, micromorphologic, isotopic and palynologic data. Results point to environmental changes, with ages that coincide with Marine Isotopic Stages (MIS) 5b; 3; 2 and 1. During the late Pleistocene, although under temperatures and precipitation lower than today, the local record points to relatively wet local environments, where shallow soil-water saturated zones contributed to erosion and sedimentation during periods of climatic change, as during the transition between MIS 2 and MIS 1. Late Pleistocene events with ages that coincide with the Northern Hemisphere Younger Dryas are also depicted. During the mid Holocene, slope-wash deposits suggest a climate drier than today, probably under the influence of seasonally contrasted precipitation regimes. The predominance of overland flow-related sedimentary deposits suggests an excess of precipitation over evaporation that influenced local palaeohydrology. This environmental condition seems to be recurrent and explains how slope morphology had influenced pedogenesis and sedimentation in the study area. Due to relative sensitiveness, resilience and short source-to-sink sedimentary pathways, near-valley head sites deserve further attention in Quaternary studies in the humid tropics.  相似文献   

Evaluating grain sorghum hybrids for tolerance to iron chlorosis     

Augustine Obour  Alan Schlegel  Ramasamy Perumal  Johnathon Holman  Dorivar Ruiz Diaz 《Journal of plant nutrition》2019,42(4):401-409

Grain sorghum production in calcareous soils is frequently affected by iron (Fe) chlorosis. Greenhouse experiments were conducted to screen sorghum hybrids for their tolerance to iron deficiency chlorosis (IDC) and evaluate the effectiveness of Fe chelate application in alleviating IDC. Treatments in Exp. 1 were a factorial combination of 14 sorghum hybrids and three Fe chelate application rates (0, 3.4 and 6.8?kg product ha^?1) applied in-furrow with the seed at the time of planting. Exp. 2 evaluated two sorghum hybrids (85Y40 and NK5418) and three Fe chelate rates (0 and 3.4?kg product ha^?1) at planting, and a split treatment of 3.4?kg ha^?1. Results showed iron chelate application suppressed IDC and increased leaf chlorophyll content and grain yield in susceptible hybrids. Split application of Fe chelate suppressed IDC and increased grain yield. Our results indicate sorghum hybrids G8G08, 86G32 and 87P06 showed promise for tolerance to IDC.  相似文献   

Severe life‐threatening hypokalemia in a cat with suspected distal renal tubular acidosis     

Carlos Torrente DVM  MS  Paolo Silvestrini DVM  Rafael Ruiz de Gopegui DVM  PhD  DECVIM 《Journal of Veterinary Emergency and Critical Care》2010,20(2):250-257

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Effect of climatic conditions on the overcoming of dormancy in apricot flower buds in two Mediterranean areas: Murcia (Spain) and Tuscany (Italy)     

R. Viti  L. Andreini  D. Ruiz  J. Egea  S. Bartolini  C. Iacona  J.A. Campoy 《Scientia Horticulturae》2010

For temperate-zone fruit species such as apricot, when winter cold requirements are not adequately satisfied, negative repercussions on productivity occur. The aim of this research was to study the effect of, mainly temperature on the overcoming of dormancy in different apricot cultivars growing in two areas (Italy, Tuscany: lat. 43°02′N, long. 10°36′E; Spain, Murcia: lat. 38°16′N, long. 1°16′W) representative of the Mediterranean climate. Trials were conducted for two consecutive years on the same genotypes: ‘Currot’, ‘San Castrese’, ‘Goldrich’, ‘Stark Early Orange’ and ‘Orange Red’. These genotypes cover the range of chilling requirements (CR) in the apricot species in these Mediterranean areas. The CR (measured by chill units, CU and portions) for breaking of dormancy, heat requirements, flowering date and flowering and fruit-set percentages were measured. Temperatures were recorded and transformed into the corresponding CU and portions by the Utah Model and the Dynamic model, respectively. The winter climatic conditions determined a dissimilar chill unit accumulation in Tuscany (Italy) and Murcia (Spain) as well as an important effect of the year in both areas. While all cultivars with the exception of Stark Early Orange overcame dormancy, significant differences regarding the CR of cultivars for two different years and growing in two environments were observed, depending also on the cultivar. The results showed that both the Utah Model and Dynamic Model were not completely accurate with regard to establishing the CR for dormancy release under a Mediterranean climate. Temperature should be analysed together with other climatic factors in order to improve the CR assessment.  相似文献   

Physicochemical,Nutritional and Antibacterial Characteristics of the Fruit of <Emphasis Type="Italic">Bromelia pinguin</Emphasis> L.     

Juan Fernando Pío-León  Gabriela López-Angulo  Octavio Paredes-López  Magdalena de Jesús Uribe-Beltrán  Sylvia Páz Díaz-Camacho  Francisco Delgado-Vargas 《Plant foods for human nutrition (Dordrecht, Netherlands)》2009,64(3):181-187

Bromelia pinguin L. is a plant native to Sinaloa, Mexico, where its fruit is used as food or as a phytotherapeutic agent. The fruits of B. pinguin were characterized and they could be considered as a functional food. These fruits show an average weight of 13.7 g and a yellow color of high luminosity (b* = 43.2, L* = 74.5). The values for acidity (4.6%, as citric acid) and pH (3.7) of B. pinguin fruit are similar to those of citrics. The edible portion is characterized by a high content of vitamin C (126 mg/100 g), ash (10.6 g/100 g d.w.), crude fiber (3.4 g/100 g d.w.), calcium (1290 mg/100 g d.w.), magnesium (500 mg/100 g d.w.), manganese (2.95 mg/100 g d.w.) and it is a good source of zinc (2.8 mg/100 g d.w.). Polar fractions extracted from the pulp fruit showed activity against several genera of human pathogenic-bacteria (Staphylococcus, Streptococcus, Enterococcus, Salmonella, Shigella, Escherichia and Pseudomonas).  相似文献   

Genetic analyses for bioactive potential in tetraploid potato (Solanum tuberosum L.)          下载免费PDF全文

Roberto Tierno  Jose Ignacio Ruiz de Galarreta 《Plant Breeding》2018,137(2):210-219

A total of four traits related to bioactive potential of the tetraploid potato were studied in a North Carolina II cross‐experiment. The objectives of this study were to estimate genetic parameters for target bioactive compounds in a tetraploid potato population, to determine phenotypic and genotypic correlations and to calculate combining ability effects of cultivars and breeding lines included in the crossing scheme. Total monomeric anthocyanins, phenolics, carotenoids and hydrophilic antioxidant capacity were measured by spectrophotometric methods. The sources of variation female (GCA_f), male (GCA_m) and their interaction (SCA) were significant for bioactive compounds and antioxidant capacity. Narrow‐sense heritability estimates (h²) were moderate for all the traits (0.413–0.657). Both phenotypic and genotypic correlations were highly significant for phenolic compounds and antioxidant capacity. As regards combining ability, positive and negative GCA and also SCA effects have been identified for bioactive compounds and antioxidant capacity. These results indicate that hybrid breeding lines can be obtained by choosing parents with significant GCA, whose bioactive potential can also be further enhanced if the crosses have a significant SCA.  相似文献   

Seasonal changes of mercury speciation in the coastal sediments     

Jacek Bełdowski  Marta Szubska  Magdalena Bełdowska  Katarzyna Jankowska  Ewa Kotlarska  Bożena Graca 《Journal of Soils and Sediments》2018,18(12):3424-3436

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Raffinose family oligosaccharides in seeds of <Emphasis Type="Italic">Pisum</Emphasis> wild taxa,type lines for seed genes,domesticated and advanced breeding materials     

Magdalena Gawłowska  Wojciech Święcicki  Lesław Lahuta  Zygmunt Kaczmarek 《Genetic Resources and Crop Evolution》2017,64(3):569-578

The content of raffinose family oligosaccharides (RFOs) in pea seeds constrains their usage in feeding humans and animals. In our research, the content of soluble carbohydrates—particularly α-d-galactosides of sucrose (RFOs)—was analyzed. The materials were as follows: 248 accessions from the Polish Pisum Genebank including representatives of taxa (from species to convarietas), type lines for genes controlling seed characters, and breeding materials and cultivars. Accessions were divided into homogeneous groups considering content of total soluble carbohydrates, total RFOs and individual RFOs: raffinose, stachyose and verbascose. The highest content of total soluble carbohydrates and total RFOs were stated for accessions with wrinkled seeds (r and rb genes) and the lowest content for seeds of the wild species P. fulvum Sibth. et Sm. Accessions valuable for breeding (for further decreasing of anti-nutritional compounds) were found among domesticated taxa (P. sativum subsp. sativum convar. vulgare Alef. and speciosum (Dierb.) Alef.), breeding lines, and some wild taxa. Accessions with decreased content of a total RFOs and verbascose are particularly valuable. It was found that the content of total RFOs was the most highly, frequently, and positively correlated with a stachyose and verbascose content. However, in P. fulvum seeds with the lowest content of RFOs and verbascose, a high correlation between the content of total RFOs and stachyose was revealed. Contents of all oligosaccharides were substantially lower in lines with dominant alleles of main pea seed genes (R, A, and I). It can be assumed that wild, primitive peas were characterized by not-all-to-high (rather not high) content of oligosaccharides; then recessive mutations in mentioned genes resulted in an increased content of RFOs. It seems to be an interesting observation from an evolutionary point of view.  相似文献