Effects of processing on immunoreactivity of cashew nut (Anacardium occidentale L.) seed flour proteins     

Venkatachalam M  Monaghan EK  Kshirsagar HH  Robotham JM  O'Donnell SE  Gerber MS  Roux KH  Sathe SK 《Journal of agricultural and food chemistry》2008,56(19):8998-9005

Cashew nut seeds were subjected to processing including autoclaving (121 degrees C for 5, 10, 20, and 30 min), blanching (100 degrees C for 1, 4, 7, and 10 min), microwave heating (1 and 2 min each at 500 and 1000 W), dry roasting (140 degrees C for 20 and 30 min; 170 degrees C for 15 and 20 min; and 200 degrees C for 10 and 15 min), gamma-irradiation (1, 5, 10, and 25 kGy), and pH (1, 3, 5, 7, 9, 11, and 13). Proteins from unprocessed and processed cashew nut seeds were probed for stability using anti-Ana o 2 rabbit polyclonal antibodies and mouse monoclonal antibodies directed against Ana o 1, Ana o 2, and Ana o 3 as detection agents. Results indicate that Ana o 1, Ana o 2, and Ana o 3 are stable regardless of the processing method to which the nut seeds are subjected.  相似文献   

Akt/FOXO3a/SIRT1-mediated cardioprotection by n-tyrosol against ischemic stress in rat in vivo model of myocardial infarction: switching gears toward survival and longevity     

Samuel SM  Thirunavukkarasu M  Penumathsa SV  Paul D  Maulik N 《Journal of agricultural and food chemistry》2008,56(20):9692-9698

Moderate consumption of wine has been associated with decreased risk of cardiovascular events. Recently we have shown that white wine is equally as cardioprotective as red wine. However, unlike resveratrol (polyphenol in red wine), the white wine component, n-tyrosol [2-(4-hydroxyphenyl)ethanol] has not been explored for its cardioprotective effect and mechanism of action. Therefore, the present study was designed to evaluate the effect of tyrosol treatment (5 mg/kg/day for 30 days) on myocardial ischemic stress in a rat in vivo model of Myocardial Infarction (MI) and to identify key molecular targets involved in this mechanism. MI was induced by Left Anterior Descending (LAD) coronary artery ligation. Reduced infarct size (32.42 vs 48.03%) and cardiomyocyte apoptosis (171 vs 256 counts/100 HPF) were observed along with improvement in the myocardial functional parameters such as LVIDs (5.89 vs 6.58 mm), ejection fraction (51.91 vs 45.09%), and fractional shortening (28.46 vs 23.52%) as assessed by echocardiography in the tyrosol-treated animals when compared to the nontreated controls. We have also observed significant increase in the phosphorylation of Akt (1.4-fold), eNOS (3-fold) and FOXO3a (2.6-fold). In addition, tyrosol induced the expression of longevity protein SIRT1 (3.2-fold) in the MI group as compared to the non-treated MI control. Therefore tyrosol's SIRT1, Akt and eNOS activating power adds another dimension to the white wine research, because it adds a great link to the French paradox. In conclusion these findings suggest that tyrosol induces myocardial protection against ischemia related stress by inducing survival and longevity proteins that may be considered as anti-aging therapy for the heart. However, human intervention studies would be necessary before establishing any recommendations about dietary habits for tyrosol intake or administration of dietary supplements containing tyrosol.  相似文献   

Antigenic stability of pecan [Carya illinoinensis (Wangenh.) K. Koch] proteins: effects of thermal treatments and in vitro digestion     

Venkatachalam M  Teuber SS  Peterson WR  Roux KH  Sathe SK 《Journal of agricultural and food chemistry》2006,54(4):1449-1458

Rabbit polyclonal antibody-based inhibition ELISA as well as immunoblotting analyses of proteins extracted from variously processed pecans (cv. Desirable) indicate that pecan proteins are antigenically stable. Pecan antigens were more sensitive to moist heat than dry heat processing treatments. SDS-PAGE and immunoblotting analysis of the native and heat-denatured proteins that were previously subjected to in vitro simulated gastric fluid digestions indicate that stable antigenic peptides were produced. Both enzyme-to-substrate ratio and digestion time were influential in determining the stability of pecan polypeptides. The stable antigenic polypeptides may serve as useful markers in developing assays suitable for the detection of trace amounts of pecans in foods.  相似文献   

Results of a phase II clinical trial on the use of ifosfamide for treatment of cats with vaccine-associated sarcomas     

Rassnick KM  Rodriguez CO  Khanna C  Rosenberg MP  Kristal O  Chaffin K  Page RL 《American journal of veterinary research》2006,67(3):517-523

OBJECTIVE: To determine clinical activity and toxic effects of ifosfamide when used to treat cats with vaccine-associated sarcoma (VAS). ANIMALS: 27 cats with a nonresectable, recurrent, or metastatic VAS. PROCEDURE: Each cat received ifosfamide (900 mg/m(2) of body surface area) as an IV infusion during a 30-minute period. Diuresis by infusion of saline (0.9% NaCl) solution and administration of mesna were used to prevent urothelial toxicosis. Treatments were administered every 3 weeks, and tumor response was assessed after the second treatment. All ifosfamide-associated toxic effects were graded in accordance with predetermined criteria. RESULTS: 61 treatments were administered to 27 cats (median, 2 treatments/cat; range, 1 to 4 treatments/cat). After ifosfamide treatment, 1 cat had a complete response and 10 had partial responses for an overall response rate of 11 of 27 (41%; 95% confidence interval [CI], 25% to 59%). Responses lasted from 21 to 133 days (median, 70 days; 95% CI, 60 to 113 days). The acute dose-limiting toxicosis was neutropenia, which was detected 5 to 28 days (median, 7 days) after treatment. Median nadir neutrophil count was 1,600 cells/muL (range, 200 to 5,382 cells/microL). Nine (33%) cats had adverse gastrointestinal effects (primarily salivation during the ifosfamide infusion and inappetence after treatment). Two cats were euthanatized because of severe nephrotoxicosis, and 1 cat developed pulmonary edema during diuresis. CONCLUSIONS AND CLINICAL RELEVANCE: Ifosfamide has antitumor activity against VAS in cats and is tolerated well by most cats. Ifosfamide should be evaluated as an adjuvant treatment for cats with VAS.  相似文献   

Chemical composition of selected edible nut seeds     

Venkatachalam M  Sathe SK 《Journal of agricultural and food chemistry》2006,54(13):4705-4714

Commercially important edible nut seeds were analyzed for chemical composition and moisture sorption. Moisture (1.47-9.51%), protein (7.50-21.56%), lipid (42.88-66.71%), ash (1.16-3.28%), total soluble sugars (0.55-3.96%), tannins (0.01-0.88%), and phytate (0.15-0.35%) contents varied considerably. Regardless of the seed type, lipids were mainly composed of mono- and polyunsaturated fatty acids (>75% of the total lipids). Fatty acid composition analysis indicated that oleic acid (C18:1) was the main constituent of monounsaturated lipids in all seed samples. With the exception of macadamia, linoleic acid (C18:2) was the major polyunsaturated fatty acid. In the case of walnuts, in addition to linoleic acid (59.79%) linolenic acid (C18:3) also significantly contributed toward the total polyunsaturated lipids. Amino acid composition analyses indicated lysine (Brazil nut, cashew nut, hazelnut, pine nut, and walnut), sulfur amino acids methionine and cysteine (almond), tryptophan (macadamia, pecan), and threonine (peanut) to be the first limiting amino acid as compared to human (2-5 year old) amino acid requirements. The amino acid composition of the seeds was characterized by the dominance of hydrophobic (range = 37.16-44.54%) and acidic (27.95-33.17%) amino acids followed by basic (16.16-21.17%) and hydrophilic (8.48-11.74%) amino acids. Trypsin inhibitory activity, hemagglutinating activity, and proteolytic activity were not detected in the nut seed samples analyzed. Sorption isotherms (Aw range = 0.08-0.97) indicated a narrow range for monolayer water content (11-29 mg/g of dry matter). No visible mold growth was evident on any of the samples stored at Aw < 0.53 and 25 degrees C for 6 months.  相似文献   

Impact of low potassium fertilization on potassium transformation under different crop management systems in western plain of arid India     

Sunil Kumar  I. J. Gulati  S. R. Yadav  R. S. Yadav  P. C. Moharana  R. L. Meena 《Journal of plant nutrition》2018,41(4):411-424

Acquaintance of potassium (K) transformation in soil is crucial for K management in management system of arid India. Present study revealed that the extractable K like ammonium acetate–potassium (NH₄OAc–K), nitric acid (HNO₃)–K, and hydrochloric acid (HCl)–K under different systems were followed the order: irrigated low input > rainfed low input > irrigated high input > no input. The pearl millet systems in irrigated low input practice maintained a higher NH₄OAc–K than irrigated high input practice. The exchangeable-K (Exch-K), non-exchangeable-K (Non-exch K) and lattice-K were observed highest in irrigated low input system and lowest in no input system. It is noticed that Exch-K contributed 2.3–2.6% over total-K, which readily available to plants. The Exch-K was highly significant correlated with K pools and soil properties, indicating that rapid establishment of equilibrium between the pools. Thus, knowing K reserve and distribution of K forms can help in understanding K replenishment capacity of arid soil under long-term intensive cultivation.  相似文献   

Depth dynamics of soil N contents and natural abundances of 15N after 43 years of long-term fertilization and liming in sub-tropical Alfisol     

Avijit Ghosh  Brahma Swaroop Dwivedi  Dipak Ranjan Biswas  Mahesh Chandra Meena  Abhijit Sarkar 《Archives of Agronomy and Soil Science》2018,64(9):1290-1301

The aim of this study was to understand impacts of long-term (43 years) fertilization on soil aggregation, N accumulation rates and δ¹⁵N in surface and deep layers in an Alfisol. Soil samples from seven treatments were analysed for N stocks, aggregate-associated N in 0–30 cm and the changes in δ¹⁵N in 0–90 cm depths. The treatments were: unfertilized control (control); recommended N dose (N); recommended N and phosphorus doses (NP); recommended N, P and potassium doses (NPK); 150% of recommended N, P and K doses (150% NPK); NPK + 10 Mg FYM ha^?1 (NPK + FYM) and NPK + 0.4 Mg lime ha^?1 (NPK + L). Results revealed that plots under NPK + FYM had ~39% higher total N concentrations than NPK + L in 0–30 cm soil layers. In NPK + L, macro-aggregates had 35 and 11% and microaggregates had 20 and 9% lower δ¹⁵N values than NPK + FYM in 0–15 and 15–30 cm soil layers, respectively. However, plots receiving NPK + FYM had ~39% greater deep soil (30–90 cm) N accumulation than NPK + L. These results would help understanding N supplying capacity by long-term fertilization and assist devising N management strategies in sub-tropical acidic Alfisols.  相似文献   

Agroecology as a Climate Change Adaptation Strategy for Smallholders of Tehri-Garhwal in the Indian Himalayan Region     

Rajiv Pandey  Roberta Aretano  Ajay K. Gupta  Dhanraj Meena  Bhuvnesh Kumar  Juha M. Alatalo 《Small-Scale Forestry》2017,16(1):53-63

Climate change is expected to increase temperatures and rainfall in the Himalayan region and place stress on local livelihoods by affecting agro-biodiversity, crop yield, cropping patterns and the species composition of forests. This paper reports findings of a survey of farmers into the role of agroforestry or ‘agroecological intensification’ for reducing climate change vulnerability. The survey was conducted in the Tehri-Garhwal district of Uttarakhand, a Himalayan State of India. Existing traditional agroforestry was found to provide ecosystem services to farmers at various scales ranging from the global to household level, contributing to the livelihoods and resilience of the farmers to climate change. The strategies of farmers for adaptating to climate change include adoption of agroforestry due to its diversified agricultural and forest products and services. The analysis suggests that policies should consider agroforestry as a tool for improving the livelihood and resilience to climate change of farmers.  相似文献   

Nature of Acidity in Alfisols,Entisols and Inceptisols in Relation to Soil Properties     

Javid Ahmad Bhat  Biswapati Mandal  Gora Chand Hazra 《Communications in Soil Science and Plant Analysis》2017,48(4):395-404

Twenty acid soils of West Bengal, India, representing Alfisols, Entisols and Inceptisols orders, were studied to characterize their acidity in relation to physicochemical properties. Total potential acidity (TPA), pH-dependent acidity (PDA), total acidity (TA), hydrolytic acidity (HA) and exchange acidity (EA) ranged from 2.02–6.90, 1.75–6.05, 1.18–2.75, 0.98–1.90 and 0.06–0.85 cmol(p⁺) kg^?1, respectively. Relative order for all forms of acidity was: Entisols>Alfisols>Inceptisols. Average contribution of EA to TPA and TA was 9.7% and 19.7%, respectively, and that of PDA to TPA was 90.1%. Contribution of electrostatically bound H⁺ to EA was highest for Inceptisols followed by Entisols>Alfisols, and reverse was true for electrostatically bound aluminum (Al³⁺). All forms of acidity showed significant positive correlations with organic carbon (C) forms of Al but negative correlations with pH of soil. They also showed significant correlations with each other. Soil pH, organic C and exchangeable Al caused most of the variations in different forms of soil acidity.  相似文献   

Improvement in Productivity,Water-Use Efficiency,and Soil Nutrient Dynamics of Summer Peanut (Arachis hypogaea L.) through Use of Polythene Mulch,Hydrogel, and Nutrient Management     

N. K. Jain  H. N. Meena 《Communications in Soil Science and Plant Analysis》2017,48(5):549-564

Agronomic management through better use of inputs benefits farmers both by enhancing productivity and profitability. A field experiment was conducted in consecutive summer seasons (2011–2013) consisting of two mulching (no mulch, polythene mulch), three hydrogel (0, 2.5, 5.0 kg ha^?1), and three nutrient management treatments (organic, inorganic, and integrated) in a split–split plot design. Use of mulching and 2.5 kg hydrogel ha^?1 and integrated nutrient management enhanced pod, haulm, kernel and oil yields, and net economic returns. Partial factor productivity and water-use efficiency were higher under polythene mulch and 5.0 kg hydrogel ha^?1. Higher nutrient uptakes were obtained under both mulching and integrated nutrient management. Use of 2.5 kg hydrogel ha^?1 resulted in more removal of N; P and K uptakes were higher in 5.0 kg hydrogel ha^?1. Combination of three managements had a consequence of actual soil N loss, but gains in soil P and K after three cropping cycles.  相似文献