Effect of Pre‐Treatment of Ovine Sperm on Male Pronuclear Formation and Subsequent Embryo Development Following Intracytoplasmic Sperm Injection     

A Shirazi  M Derakhshan‐Horeh  AA Pilvarian  E Ahmadi  H Nazari  B Heidari 《Reproduction in domestic animals》2011,46(1):87-94

The objective of this study was to determine the effects of various methods of sperm pre‐treatment on male pronuclear (MPN) formation and subsequent development of ovine embryos derived from in vitro‐matured oocytes and intracytoplasmic sperm injection (ICSI). The effect of treatment of injected oocytes with dithiothreitol (DTT) on embryo development was also assessed. In Exp. 1, the injected oocytes with non‐treated sperm were activated with three different procedures. The cleavage and blastocyst rates in those activated with DTT was lower (p < 0.05) than those activated with either ionomycin (Io) + 6‐dimethylaminopurine (6‐DMAP) or DTT + I + 6‐DMAP. In Exp. 2, the effects of sperm pre‐incubated with DTT, sodium dodecyl sulphate (SDS) or DTT + SDS as well as two‐time frozen/thawed sperm (without cryoprotectant) on MPN formation and oocyte activation were examined. The non‐treated sperm served as controls. The MPN formation in DTT + SDS group was higher (p < 0.05) than other groups except for freeze–thaw group. No difference in the rate of activated ICSI oocytes was observed among groups. In Exp. 3, the effect of pre‐treatment of sperm on subsequent development of ICSI embryos and blastocyst cell numbers were examined. The rates of cleavage and blastocyst formation as well as the blastocyst cell numbers were similar among the pre‐treated and control groups. In conclusion, pre‐treatment of sperm with DTT + SDS positively affected MPN formation, although the subsequent development capacity of the resulting embryos remained limited. Moreover, DTT was not effective on oocyte activation compared with Io + 6‐DMAP after ICSI.  相似文献   

Study of infection with an Iranian field-isolated H9N2 avian influenza virus in vaccinated and unvaccinated Japanese quail     

Ebrahimi SM  Ziapour S  Tebianian M  Dabaghian M  Mohammadi M 《Avian diseases》2011,55(2):195-200

In the present study, we examined the mortality rate, egg production, and clinical signs of quail experimentally infected with a field isolate of A/Chicken/Iran/339/02 (H9N2) avian influenza virus obtained from an infected commercial layer farm with severe morbidity and mortality. A total of 120 quail at 14 days old were randomly divided into four groups of vaccinated (B and C) and unvaccinated (A and D) birds. Vaccination was done on days 20 and 32, and viral inoculation of birds in groups C and D was then carried out on day 43. For evaluation of viral transmission, at 24 hr postinoculation additional unvaccinated birds were placed in direct contact with challenged birds. All the birds were evaluated for clinical signs, egg production, antibody production, viral titration in lung homogenates, and viral transmission following inoculation. All unvaccinated-challenged birds were infected and showed clinical signs, whereas the infection rate along with clinical signs of vaccinated-challenged birds reached 30%-40%. Although vaccination induced high antibody titers, reduction in food and water consumption was evident in this vaccinated-challenged group compared with the unchallenged control group. These results could indicate that inactivated vaccine did not fully prevent the infection, although it was capable of protecting birds against clinical signs and significantly decreased viral titers in lungs after intranasal challenge.  相似文献   

Molecular genotyping and epidemiology of Mycobacterium bovis strains obtained from cattle in Iran     

Mosavari N  Feizabadi MM  Jamshidian M  Shahpouri MR  Forbes KJ  Pajoohi RA  Keshavarz R  Taheri MM  Tadayon K 《Veterinary microbiology》2011,151(1-2):148-152

Restriction fragment length polymorphism (RFLP) genotyping was employed to analyze the population genetics of Mycobacterium bovis in Iran. One hundred and twenty-three isolates collected from slaughtered tuberculosis-suspect cattle and one clinically asymptomatic buffalo were subjected to RFLP analysis with probes of the polymorphic GC-rich sequence (PGRS) and the direct repeat sequence (DR) using DNA digested with PvuII and AluI. All these methods detected a large homogeneous population in which only a few isolates had variant genotypes. Only AluI-based RFLPs of both the PGRS and DR sequences were able to clearly differentiate between BCG and field strains of M. bovis. As in previous reports, these findings seem to reflect a recent dispersal of one or a few strains in Iran following the substantial expansion of Holstein-Friesian cattle over the last few decades.  相似文献   

Taurine is more effective than melatonin on cytochrome P450 2E1 and some oxidative stress markers in streptozotocin-induced diabetic rats     

El-Batch M  Hassan AM  Mahmoud HA 《Journal of agricultural and food chemistry》2011,59(9):4995-5000

Melatonin and taurine have alleviative effects in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats were divided into nondiabetic, diabetic, diabetic melatonin supplemented and diabetic taurine supplemented groups. At the end of the study, both blood and liver were collected for determination of some oxidative stress parameters, and hepatic cytochrome P450 2E1 (CYP2E1) enzyme activity and gene expression. An increased CYP2E1 activity and expression level with a concomitant significant change in oxidative stress parameters were found in STZ-induced diabetic rats. Taurine or melatonin supplementation to the diabetic rats alleviated these experimental parameters with a more significant effect for taurine than that of melatonin. Suppression of β-hydroxybutyrate (β-HB) production by taurine can be one of the mechanisms of a reduction in CYP2E1. Taurine was effective more than melatonin in reducing CYP2E1 activity and expression; therefore antioxidants might prove beneficial in type 1 diabetes associated with manifestations of liver injury.  相似文献   

Folic acid and flaxseed oil supplements in Ossimi ewes: effect on body weight changes,progesterone profile,blood chemistry,and litter traits     

El-Tarabany  Mahmoud S.  Atta  Mostafa A.  Emara  Sana S.  Mostafa  Mohamed M. 《Tropical animal health and production》2020,52(1):301-308

Tropical Animal Health and Production - The aim was to explore the impact of periconceptional folic acid or flaxseed oil (FXO) supplementation on fertility, progesterone profile, and blood...  相似文献   

Animal rabies situation in Sultanate of Oman (2017–2019)     

El-Neweshy  Mahmoud S.  Al Mayahi  Nada  Al Mamari  Wafa  Al Rashdi  Zahra  Al Mawly  Julanda H. 《Tropical animal health and production》2020,52(6):3069-3076

Successful preventive and control measures of zoonotic diseases require updated epidemiological data. Sylvatic rabies is endemic in Oman since 1990. Studying of the prevalence of animal rabies in Oman (2017–2019) was the goal of the current study besides the clinical–histopathological investigations of rabies in different animal species. A total of 117 whole brains of different animal species from different regions of Oman were examined by fluorescent antibody test (FAT) and histopathology for rabies during 2017–2019. Sixty-four samples (54.7%) were positive for rabies by FAT. The most affected species were goat (53.1%) followed by camel (18.8%), which pose a great risk to farmers and veterinarians. Positive fox cases were (10.9%). Most confirmed cases of animal rabies were submitted from Northern regions of Oman. Rabies was reported recently in Al Wusta among wild ruminants, Central Oman. The seasonal cycle of animal rabies in Oman was year-round with the peak from December to April. The clinical signs and neuropathological findings were nearly similar in different animal species. Histopathology-positive cases had Negri bodies in pyramidal and purkinje neurons, non-suppurative encephalitis features, and neuronal degeneration and necrosis. The sensitivity and specificity of histopathological diagnosis of rabies in different animals were 76.47% and 100.00%, respectively. Finally, sylvatic rabies remains a major challenge to the public and animal health in Oman. Although of the value of histopathological diagnosis of rabies if no other technique is available, other complementary tests must be employed to confirm negative results.

  相似文献   

Isolation of canine adipose-derived mesenchymal stem cells from falciform tissue obtained via laparoscopic morcellation: A pilot study     

Christine M. DePompeo DVM  Mariana Ianello Giassetti DVM  PhD  Mahmoud M. Elnaggar BVSc  MVSc  PhD  Jon M. Oatley PhD  William C. Davis PhD  Boel A. Fransson DVM  PhD  DACVS 《Veterinary surgery : VS》2020,49(Z1):O28-O37

  相似文献   

Rice Straw Composting and Its Effect on Soil Properties     

Esawy Mahmoud  Paul Robin  Nouraya Akkal-Corfini 《Compost science & utilization》2013,21(3):146-150

In Egypt, recycling rice straw and organic wastes is of great concern as well as improvement of soil properties. Rice straw compost could improve both organic waste recycling and soil quality. The aim of this study was to evaluate the effect of the rice straw compost, with or without water treatment residuals (WTR), on soil chemical properties and dry weight of canola. The results showed that the addition of rice straw compost and WTR decreased soil salinity and increased Ca⁺², K⁺ and organic matter. The addition of compost and WTR (2:1 wet weight ratio) at level of 10 g dry weight kg^?1 dry soil gave the best reduction in soil salinity compared to compost or WTR only or for level 10 g dry weight kg^?1 dry soil. The results also showed that the available P decreased with the application of WTR while it increased with the application of compost. The study demonstrated that the dry weight and relative increase (R.I %) of dry weight of canola plants increased with the application of WTR and compost to soil. Combinations of WTR and compost to soils had a greater effect on increasing yield and improved the efficiency of WTR on soil properties.  相似文献   

Nephrotoxicity and oxidative stress of single large dose or two divided doses of gentamicin in rats     

Kandeel M  Abdelaziz I  Elhabashy N  Hegazy H  Tolba Y 《Pakistan journal of biological sciences: PJBS》2011,14(11):627-633

Gentamicin (GS) is a potent antimicrobial exhibiting concentration dependent bacterial killing. A high dose ofgentamicin (10 mg kg(-1)) is required to reach sufficient concentrations in specific fluids as cerebrospinal fluid and to be effective on antibiotics resistant bacteria as well as treatment of acute and dangerous illness. Using a rat model, the renal toxicity and oxidative stress of administering gentamicin (10 mg kg(-1) daily for 7 days) either in a single dose or divided into 2 doses was investigated. The safety of dose regimens was assessed through oxidant-antioxidant parameters as well as renal function tests. Typical renal damage and high oxidative stress were evident in the control group receiving 100 mg kg(-1) gentamicin daily for 7 days. This was verified by high serum urea, uric acid, creatinine as well as increase in the levels of oxidative stress biomarkers as malondialdehyde, NO, total antioxidant capacity and decrease in reduced glutathione level. At any of the used regimen, 10 mg kg(-1) gentamicin did not provide high compromise for renal functions nor significantly increased the oxidative stress and tissue damage. Based on microscopic lesions scores and biochemical analysis, there were no significant differences between single or two divided dosages of gentamicin at dose rate of 10 mg kg(-1) day(-1). Further studies are required for applications in other animals of human subjects.  相似文献   

Pluripotent stem cells: a new approach to tendon therapy     

Kholif Ael-K 《Pakistan journal of biological sciences: PJBS》2011,14(22):1032-1033

  相似文献