Homoeological relationships between the f chromosome of Brassica rapa and the e chromosome of Brassica oleracea   总被引：1，自引：0，他引：1  

Y. Kaneko    N. Nagasawa    S. W. Bang  Y. Matsuzawa 《Plant Breeding》2002,121(2):171-173

Eight plants of the putative double monosomic addition line (DMAL, 2n= 20) were developed by crossing a monosomic chromosome addition line of radish [f(A)‐type monosomic addition line (MAL) (2n= 19)] carrying the f chromosome of Brassica rapa (2n= 20, AA) with another [e(C)‐type MAL (2n= 19)] having the echromosome of Brassica oleracea (2n= 18, CC). The homoeological relationships between the two alien chromosomes were investigated by morphological, cytogenetic and random amplified polymorphic DNA (RAPD) analysis. Seventeen morphological traits that were not present in the radish cv. ‘Shogoin’ were observed in both MALs and these traits were substantially exhibited in DMAL plants. At the first metaphase of pollen mother cells (PMCs), the two parental MALs showed a chromosome configuration of 9II +1I, demonstrating impossibility of recombination between the R and the added chromosomes. The DMALs formed 10II in approximately 73% of PMCs, with one bivalent showing loose pairing between two chromosomes differing in size. In an attempt to identify the two MALs by RAPD‐specific markers using 26 selected random primers, 13 and 20 bands were specific for the f(A)‐type and the e(C)‐type MALs, respectively; 12 bands were common to both MALs (26.7%). In conclusion, the f chromosome of B. rapa is homoeologous to the e chromosome of B. oleracea. The genetic domain (genes) for 17 morphological traits are linked to each homoeologous chromosome bearing 27% of the corresponding RAPD markers.  相似文献   

Influence of soil quality on the growth of Folsomia candida (Willem) (Collembola)     

Satoshi Kaneda  Nobuhiro Kaneko 《Pedobiologia》2002,46(5):428-439

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Naidid worms (Oligochaeta, Naididae) in paddy soils as affected by the application of legume mulch and/or tillage practice     

Hiroshi Yokota  Nobuhiro Kaneko 《Biology and Fertility of Soils》2002,35(2):122-127

Reproduction, intrinsic rate of natural increase and population density of naidid worms were investigated in submerged paddy fields and the laboratory. No tillage plus legume-mulching increased the population density of naidid worms. Soil treatments with neither tillage nor legume mulch, and tillage practice alone, did not increase the number of worms. Dero dorsalis Ferronnière was dominant in soil of the no-tillage treatment. In laboratory experiments, legume-mulching with the proper amount of dissolved O₂ accelerated asexual reproduction of D. dorsalis through zooid budding. For the legume and aeration treatment, (N_i+1-N_i) N_i^-1 values (where N_i and N_i+1 are the populations at times t=i and t=i+1) were plotted against N_i+1. Utilizing this linear relation, this data fitted the logistic curve (r²=0.885, P<0.05). Based on the linear relation, the intrinsic rate of natural increase (r), carrying capacity (K), and doubling time (T) were calculated as 0.2125 day^-1, 12,666 m^-2, and 3.26 days, respectively. The amounts of legumes applied were highly correlated with the population of D. dorsalis, indicating that the weight of legume is a limiting factor with respect to carrying capacity. A literature review indicated a significant correlation (P<0.01) between intrinsic rate of natural increase and maximum body length of naidids with temperature conversion of the growth rate. Sexually mature worms were rarely found in submerged paddy fields. Sexual reproduction seems to be adopted in response to soil desiccation after paddy field drainage.  相似文献   

Essential role of Fkbp6 in male fertility and homologous chromosome pairing in meiosis     

Crackower MA  Kolas NK  Noguchi J  Sarao R  Kikuchi K  Kaneko H  Kobayashi E  Kawai Y  Kozieradzki I  Landers R  Mo R  Hui CC  Nieves E  Cohen PE  Osborne LR  Wada T  Kunieda T  Moens PB  Penninger JM 《Science (New York, N.Y.)》2003,300(5623):1291-1295

Meiosis is a critical stage of gametogenesis in which alignment and synapsis of chromosomal pairs occur, allowing for the recombination of maternal and paternal genomes. Here we show that FK506 binding protein (Fkbp6) localizes to meiotic chromosome cores and regions of homologous chromosome synapsis. Targeted inactivation of Fkbp6 in mice results in aspermic males and the absence of normal pachytene spermatocytes. Moreover, we identified the deletion of Fkbp6 exon 8 as the causative mutation in spontaneously male sterile as/as mutant rats. Loss of Fkbp6 results in abnormal pairing and misalignments between homologous chromosomes, nonhomologous partner switches, and autosynapsis of X chromosome cores in meiotic spermatocytes. Fertility and meiosis are normal in Fkbp6 mutant females. Thus, Fkbp6 is a component of the synaptonemal complex essential for sex-specific fertility and for the fidelity of homologous chromosome pairing in meiosis.  相似文献   

Growth of the collembolan Folsomia candida Willem in soil supplemented with glucose   总被引：1，自引：0，他引：1  

Satoshi Kaneda  Nobuhiro Kaneko   《Pedobiologia》2004,48(2):165-170

Freely available glucose improves the conditions for soil microorganisms which are utilized as food by Collembola. We examined the effects of glucose application on collembolan (Folsomia candida Willem) growth and on several biotic and abiotic soil parameters (microbial biomass, soil respiration, qCO₂, dissolved organic carbon, inorganic nitrogen, and Olson-P) in an artificial system without predatory pressure on Collembola. Glucose addition increased soil respiration and qCO₂, and decreased nutrient levels in the soil. Collembolan growth increased with increasing glucose doses. We conclude that the availability of carbon substrates can sustain collembolan growth via an improvement of microbial growth conditions.  相似文献   

Ascorbate regeneration by the reduced form of 2-amino-3-carboxy-1, 4-naphthoquinone, a strong growth stimulator for bifidobacteria     

Yamazaki S  Iwasa K  Kano K  Ikeda T  Taketomo N  Kaneko T 《Journal of agricultural and food chemistry》2000,48(11):5643-5648

Nonenzymatic reduction of dehydroascorbate into ascorbate by the reduced form (quinol form) of 2-amino-3-carboxy-1,4-naphthoquinone, a strong growth stimulator for bifidobacteria, has been found. The bimolecular reaction rate constant was evaluated as 9 M(-)(1) s(-)(1) at pH 7.0. This reaction has been successfully coupled with enzymatic regeneration of the naphthoquinol by NAD(P)H in cell-free extracts of Bifidobacterium longum 6001. The overall reaction is a regeneration of NAD(P)(+) by dehydroascorbate [or a regeneration of ascorbate by NAD(P)H], in which the naphthoquinone/quinol redox couple functions as an electron transfer mediator. Kinetic study of the reduction of dehydroascorbate with related quinol compounds suggested the significance of the amino substituent of the naphthoquinol. A mechanism of the electron transfer from the quinol to dehydroascorbate is proposed, where the first step of the reaction is a nucleophilic addition of the C(2)-amino substituent of the naphthoquinol to the C(2)-position of dehydroascorbate to form a Schiff base intermediate.  相似文献   

Heinz body hemolytic anemia associated with the use of urinary antiseptics containing methylene blue in the cat   总被引：1，自引：0，他引：1  

R D Schechter  O W Schalm  J J Kaneko 《Journal of the American Veterinary Medical Association》1973,162(1):37-44

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Physicochemical properties of Ibaraki virus hemagglutinin     

S Tokuhisa  Y Inaba  K Sato  Y Miura  N Kaneko  M Matumoto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1983,45(5):637-646

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Book reviews     

J. J. Kaneko DVM  PhD  G. M. Robins 《Veterinary research communications》1987,11(2):199-200

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Protection of mice against parenteral and oral infection with Yersinia enterocolitica     

R Nakajima  K Kaneko  N Hashimoto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(5):721-727

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