Intranasal hemangiosarcoma in a dog     

Fujita M  Takaishi Y  Yasuda D  Hasegawa D  Taniguchi A  Takahashi K  Orima H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):525-528

Magnetic resonance (MR) was conducted for an 8-year-old, intact male Spitz with sneezing, serous discharge and epistaxis from the left nasal cavity. MR imaging showed a nasal cavity-occupied mass of iso-intensity on T1WI , high-intensity on T2WI and markedly enhanced on contrast-enhanced T1WI at parts of rostal to medial ocular angle in the left cavity. After Surgery and intraoperative radiation, the mass was diagnosed intranasal hemangiosarcoma by histopathology. Although the dog showed the finding, which suggested recurrence after the treatment ending, about 30 months later, it maintained good conditions without evidence of metastasis.  相似文献   

Generation of monoclonal antibody against canine neural-cell adhesion molecule     

Uematsu Y  Yamaguchi T  Koike A  Yagihara H  Hasegawa D  Matsuki N  Ono K  Washizu T  Arai T  Bonkobara M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):845-847

A monoclonal antibody, K9BYU, was generated using Escherichia coli recombinant extracellular domain of canine neural-cell adhesion molecule (N-CAM) as an antigen. Immunoreactivity of K9BYU to insect cell recombinant canine N-CAM was demonstrated by Western blotting using Sf9 insect cells transfected with the canine N-CAM gene. In Western blotting against canine brain tissue, K9BYU detected three isoforms of N-CAM that correspond to three major isoforms of human and mouse N-CAM (N-CAM-120, -140, and -180). From these results, K9BYU was considered to be a useful tool for research of canine N-CAM.  相似文献   

Pharmacokinetics and toxicity of zonisamide in cats     

Hasegawa D  Kobayashi M  Kuwabara T  Ohmura T  Fujita M  Orima H 《Journal of Feline Medicine and Surgery》2008,10(4):418-421

With the eventual goal of making zonisamide (ZNS), a relatively new antiepileptic drug, available for the treatment of epilepsy in cats, the pharmacokinetics after a single oral administration at 10mg/kg and the toxicity after 9-week daily administration of 20mg/kg/day of ZNS were studied in healthy cats. Pharmacokinetic parameters obtained with a single administration of ZNS at 10mg/day were as follows: Cmax=13.1microg/ml; Tmax=4.0h; T(1/2)=33.0h; areas under the curves (AUCs)=720.3microg/mlh (values represent the medians). The study with daily administrations revealed that the toxicity of ZNS was comparatively low in cats, suggesting that it may be an available drug for cats. However, half of the cats that were administered 20mg/kg/day daily showed adverse reactions such as anorexia, diarrhoea, vomiting, somnolence and locomotor ataxia.  相似文献   

Seed abortion of ‘Tosa-Buntan’ pummelo pollinated with soft-X-irradiated pollens     

Tsuneo Ogata  Tomoya Takeichi  Kazunori Matsunaga  Kojiro Hasegawa  Shinzo Yamane  Keita Sugiyama 《Scientia Horticulturae》2008

Cross-pollination was performed with soft-X-irradiated hyuga-natsu pollens (1000 Gy) for ‘Tosa-Buntan’ pummelo (Citrus grandis (L.) Osbeck). This resulted in the transformation of large and complete seeds into small and empty ones (practically seedless). Although fruit set, fruit retention, total soluble solids content (TSS) and titratable acidity of the juice were not affected, decrement in the fruit size was observed. Two weeks after the pollination, endosperm cell division with free nuclei began in both the non-irradiated and irradiated pollen treatment conditions. Seven weeks after pollination, endosperm division with the cell wall occurred in the non-irradiated pollen treatment conditions; however, the endosperm development ceased in most ovules that underwent the irradiated pollen treatment, and the ovules remained in their free nuclear stage. The delayed degeneration of the ovules, following successful fertilization and commencement of endosperm cell division, allow these seedless fruits to be categorized as pseudo-parthenocarpic.  相似文献   

Seroprevalence of Neospora caninum in dairy cattle in Bahia, Brazil.   总被引：2，自引：0，他引：2  

L F Gondim  I F Sartor  M Hasegawa  I Yamane 《Veterinary parasitology》1999,86(1):71-75

Sera collected from 447 dairy cattle on 14 dairy farms were tested for Neospora caninum antibodies by use of an immunofluorescent antibody technique. Positive reactions with titres > or =1:200 were found in 63 (14.09%) of animals. Neospora positive sera were also tested for Toxoplasma gondii antibodies by using a commercial latex agglutination test. Antibodies to T. gondii were detected in 3 (4.76%) of 63 N. caninum positive sera. These results indicate that N. caninum infection is widespread among dairy cattle in Bahia state.  相似文献   

The locus for resistance to Asian soybean rust in PI 587855          下载免费PDF全文

Naoki Yamanaka  Mio Morishita  Tomomi Mori  Yukie Muraki  Midori Hasegawa  Md. Motaher Hossain  Yuichi Yamaoka  Masayasu Kato 《Plant Breeding》2016,135(5):621-626

Asian soybean rust (ASR) caused by Phakopsora pachyrhizi is one of the most serious soybean (Glycine max) diseases in tropical and subtropical areas. A soybean line, PI 587855, showed a resistance phenotype against ASR pathogens in Japan and South America at high frequency; however, little is known of the genetic control of this resistance and chromosomal location of the corresponding locus. Therefore, the aim of this study was to study the inheritance of PI 587855 resistance and map the corresponding locus with SSR markers aiming to use the linked markers in marker‐assisted selection. In the segregating population, resistance to ASR appeared to be controlled by a single dominant gene. The ASR resistance locus was mapped near to the chromosomal region where the resistant loci, Rpp1 and Rpp1‐b, were previously mapped. Comparative genetic mapping and disease reaction profiles of other seven lines carrying Rpp1 or Rpp1‐b to four Brazilian ASR isolates revealed that the resistance reaction exhibited by PI 587855 was similar to that of Rpp1‐b‐carrying varieties which have useful resistance to South American ASR strains.  相似文献   

Starch Damage and Pasting Properties of Rice Flours Produced by Dry Jet Grinding     

Md. Sharif Hossen  Itaru Sotome  Makiko Takenaka  Seiichiro Isobe  Mitsutoshi Nakajima  Hiroshi Okadome 《Cereal Chemistry》2011,88(1):6-11

Milling method and particle size affect some properties of rice flour. To prepare ultra‐fine rice flour of <30 μm, hammer and dry jet grinding methods were examined and the effect of particle size on starch damage and pasting properties of the flour were elucidated. A jet mill could make finer flour (<10 μm mean size) with a narrower particle size distribution than a hammer mill could. Starch damage increased dramatically at a mean size of <10 μm. Particles of a similar size (<60 μm) had different levels of starch damage between mills. Not only the particle size, but also the milling method affected the level of damaged starch. Flour samples of ≥45 μm mean size had similar viscosity curves, but samples of <20 μm had different curves. Peak viscosity and final viscosity decreased sharply at <10 μm. Setback viscosity for particles of 3 μm from both brown rice and white rice were higher than the peak viscosity. Stability to heat and shearing stress were decreased for <20 μm flours as the breakdown viscosities decreased. Starch damage and pasting properties of flour ground from the nonwaxy japonica cultivar Koshihikari changed dramatically at a mean size of <10 μm.  相似文献   

Distinguishing local growth from immigration-based size shifts for juvenile chum salmon communities in coastal Hokkaido, northern Japan     

Koh Hasegawa  Tomoki Sato  Kei Sasaki 《Fisheries Science》2013,79(4):611-616

The body size of juvenile fish is often used as an index of growth rate, which in turn is influenced by local habitat conditions. We evaluated the size and origin of juvenile Chum salmon Oncorhynchus keta in the coastal areas of three regions (Atsuta, Shari, and Konbumori) of Hokkaido, northern Japan. The origin of the individuals in these communities differed between Konbumori and the other two regions. The former contained juveniles that originated from both the nearest stocked river and other rivers that were outside the area of interest. Conversely, the communities in Atsuta and Shari consisted exclusively of fish from the nearest stocked river. Moreover, the juveniles in Konbumori were larger than those in Atsuta and Shari. The results of our otolith analysis suggest that the larger size of the fish in Konbumori was due to the immigration of large individuals from natal rivers at distant locations. Thus, immigrants were likely to enlarge their body size composition in the area of interest. In summary, if the distance from a natal river is adjusted and daily growth is taken into account, body size can be used as an indicator of growth performance in coastal juvenile chum salmon.  相似文献   

Isolation of ligament protein-1 from shell ligament of the scallop Patinopecten yessoensis     

Ruihua Li  Kohji Takahashi  Tomohiko Hori  Akane Torita  Yasushi Hasegawa 《Fisheries Science》2012,78(6):1315-1320

Elastomeric proteins occur in the shell ligament of bivalve molluscan shellfish. A newly identified protein named ligament protein-1, with a molecular mass of approximately 12 kDa, was extracted from the shell ligament of the scallop, Patinopecten yessoensis, with 70 % formic acid, and purified by gel filtration column chromatography and reverse-phase C18 column chromatography. Although the amino acid composition of this protein differs from that of the known shell ligament protein abductin, the partial amino acid sequences are similar to those of abductin. CD spectra and FT–IR spectra analyses showed that ligament protein-1 retains its secondary structures of α-helix, β-turn, and β-sheets. To the best of our knowledge, this is the first study on protein purification from the shell ligament of a bivalve molluscan shell.  相似文献   

Comparisons among MRI signs,apparent diffusion coefficient,and fractional anisotropy in dogs with a solitary intracranial meningioma or histiocytic sarcoma          下载免费PDF全文

Masae Wada  Daisuke Hasegawa  Yuji Hamamoto  Yoshihiko Yu  Aki Fujiwara‐Igarashi  Michio Fujita 《Veterinary radiology & ultrasound》2017,58(4):422-432

Although MRI has become widely used in small animal practice, little is known about the validity of advanced MRI techniques such as diffusion‐weighted imaging and diffusion tensor imaging. The aim of this retrospective analytical observational study was to investigate the characteristics of diffusion parameters, that is the apparent diffusion coefficient and fractional anisotropy, in dogs with a solitary intracranial meningioma or histiocytic sarcoma. Dogs were included based on the performance of diffusion MRI and histological confirmation. Statistical analyses were performed to compare apparent diffusion coefficient and fractional anisotropy for the two types of tumor in the intra‐ and peritumoral regions. Eleven cases with meningioma and six with histiocytic sarcoma satisfied the inclusion criteria. Significant differences in apparent diffusion coefficient value (× 10^?3 mm²/s) between meningioma vs. histiocytic sarcoma were recognized in intratumoral small (1.07 vs. 0.76) and large (1.04 vs. 0.77) regions of interest, in the peritumoral margin (0.93 vs. 1.08), and in the T2 high region (1.21 vs. 1.41). Significant differences in fractional anisotropy values were found in the peritumoral margin (0.29 vs. 0.24) and the T2 high region (0.24 vs. 0.17). The current study identified differences in measurements of apparent diffusion coefficient and fractional anisotropy for meningioma and histiocytic sarcoma in a small sample of dogs. In addition, we observed that all cases of intracranial histiocytic sarcoma showed leptomeningeal enhancement and/or mass formation invading into the sulci in the contrast study. Future studies are needed to determine the sensitivity of these imaging characteristics for differentiating between these tumor types.  相似文献