Identification and some properties of anthocyanin isolated from Zuiki, stalk of Colocasia esculenta     

Terasawa N  Saotome A  Tachimura Y  Mochizuki A  Ono H  Takenaka M  Murata M 《Journal of agricultural and food chemistry》2007,55(10):4154-4159

Zuiki, a stalk of taro (Colocasia esculenta), is a traditional vegetable in Japan. Raw zuiki is often boiled and vinegared to eat. The surface color of zuiki is reddish. Here, we isolated a red pigment from zuiki and identified it as cyanidin 3-rutinoside using instrumental analyses. The color of zuiki disappeared by boiling, but the zuiki turned red again in an acetic acid solution. It seems that the cyanidin 3-rutinoside that exists on the surface of zuiki elutes in boiling water and then, the pigment that seeps out from the inside of the zuiki is exposed to an acid solution, and its surface turns red again. The radical scavenging activity of purified zuiki anthocyanin was 114 mg equivalent to BHT/g. About half of the anthocyanin in fresh zuiki was washed out by boiling, and the radical scavenging activity of zuiki was definitely reduced.  相似文献   

Comparison of behavioral responses to a novel environment between three teleosts,bluegill <Emphasis Type="Italic">Lepomis macrochirus</Emphasis>, crucian carp <Emphasis Type="Italic">Carassius langsdorfii</Emphasis>, and goldfish <Emphasis Type="Italic">Carassius auratus</Emphasis>     

Masayuki?YoshidaEmail author  Makiko?Nagamine  Kazumasa?Uematsu 《Fisheries Science》2005,71(2):314-319

To investigate the emotional reactivity of fish in a novel environment, the swimway test was developed. The swimway apparatus consists of a shaded start chamber and an open, illuminated swimway. Fish were first introduced and habituated to the start chamber. A door partitioning the start chamber from the swimway was then opened, and behavioral responses of the fish in the apparatus were measured. By using the swimway test, behavioral responses to a novel environment of bluegill Lepomis macrochirus, crucian carp Carassius langsdorfii, and goldfish Carassius auratus were quantified and compared. The emotional reactivities in blue gill were found to be the lowest and crucian carp the highest, indicating bluegill are relatively active or ‘bold’, and that the crucian carp are relatively passive or ‘shy’, in a novel environment. It is suggested that the swimway test is applicable to examning inter-species differences in relative emotional reactivity or boldness in a simplified novel situation.  相似文献   

Permeability of the fish intestinal membrane to bulky chemicals     

Chiyoko Miyata  Yoshihide Matoba  Makiko Mukumoto  Yoshiaki Nakagawa  Hisashi Miyagawa 《Journal of Pesticide Science》2022,47(2):86

The ability to predict the environmental behavior of chemicals precisely is important for realizing more rational regulation. In this study, the bioaccumulation of nine chemicals of different molecular weights absorbed via the intestinal tract was evaluated in fish using the everted gut sac method. The amounts of chemicals that passed through the intestinal membrane after a 24-hr exposure were significantly decreased for chemicals with MW≥548 and D_{max min}≥15.8 Å (or D_{max aver}≥17.2 Å). These thresholds are consistent with those previously proposed in terms of MW (>800) and molecular size (D_{max min}>15.6 Å or D_{max aver}>17.1 Å) for the limit of permeable chemicals through the gill membrane. The results show that the same MW and D_max criteria can be used to predict low bioaccumulation through both the gill membrane and the intestinal tract. These findings are helpful in reducing the need to conduct animal tests in environmental safety studies.  相似文献   

Detection of antibodies against Akabane virus in bovine sera by enzyme-linked immunosorbent assay   总被引：5，自引：0，他引：5  

S Ide  K Baba  M Tsuchimoto  H Nagano  Y Eiguchi  T Yamagami  H Yamagishi  Y Tanaka  Y Fujisaki  T Hohdatsu 《Veterinary microbiology》1989,20(3):275-280

An enzyme-linked immunonosorbent assay was established for detection of antibodies to Akabane virus in bovine sera. The assay was shown to be a useful serological tool for studies on Akabane virus infection.  相似文献   

Hepatocellular toxicosis associated with the alternate administration of carprofen and meloxicam in a siberian husky     

Nakagawa K  Yamagami T  Takemura N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(10):1051-1053

A 4-year-old female Siberian Husky was diagnosed with pyogranulomatous steatitis at the site of a recurrence of left anal sac rupture (day 1). Carprofen and orbifloxacin were given for 13 days without improvement. A single dose of meloxicam was administered prior to surgical resection of the anal sac, and based on elevated liver enzyme activity, liver supportive therapy was initiated. The dog received carprofen and orbifloxacin orally on the evening of day 14. The dog became anorectic the following morning, and began vomiting. Despite supportive therapy, the dog was unresponsive to treatment and died on day 16. Postmortem examination revealed severe vacuolar change and acute necrosis of hepatocytes consistent with carprofen and meloxicam induced-toxicosis.  相似文献   

Kid depletion in mouse oocytes associated with multinucleated blastomere formation and inferior embryo development          下载免费PDF全文

Akiyoshi Egashira  Nobuhiko Yamauchi  Md. Rashedul Islam  Kazuki Yamagami  Asami Tanaka  Hikaru Suyama  El‐Sharawy Mohamed El‐Sayed  Shoji Tabata  Takashi Kuramoto 《Animal Science Journal》2016,87(8):1048-1054

This study investigated the knockdown (KD) of Kid on maturation developmental competence and multinucleation of mouse germinal vesicle (GV) oocytes after parthenogenetic activation. Data revealed that Kid messenger RNA (mRNA) was expressed in GV and MII stage oocyte and 1‐ and 2‐cell embryos. Additionally, Kid mRNA expression in the Kid KD group decreased by nearly 46% compared to the control small interfering RNA (siRNA) groups. The rate of multinucleated embryos in the Kid KD group (52.4%) was significantly higher (P < 0.05) than the control siRNA group (4.7%). Finally, the developmental rates were significantly lower in the Kid siRNA group at > 4‐cell stage (28.6% vs. 53.5%) and the blastocyst stage (2.4% vs. 23.3%) compared to the control siRNA groups. Suppression of Kid using siRNA caused multinucleation in early embryos with high frequency and it may increase 2‐ to 4‐cell arrested embryos and reduce the developmental competence to blastocyst.  相似文献   

SSR Diversity of Vegetable Soybean [<Emphasis Type="Italic">Glycine max</Emphasis> (L.) Merr.]     

Makiko Mimura  Clarice J. Coyne  Marie W. Bambuck  Thomas A. Lumpkin 《Genetic Resources and Crop Evolution》2007,54(3):497-508

Edamame [Glycine max (L.) Merr.] is a type of soybean selected for fresh or frozen vegetable use at an immature stage. Since edamame has a similar protein content, milder flavor, nuttier texture, and is easier to cook when compared to grain soybean, it is being promoted as a new vegetable for global consumption. Global production will require breeding programs for local adaptation; however, limited research has been published on genetic diversity of edamame varieties for the assessment of genetic resources. Simple sequence repeats (SSRs) were used to study the genetic diversity among 130 accessions, including edamame cultivars and landraces from Japan, China and the US, and also the new breeding lines in the US. Although it is assumed that elite edamame cultivars would have narrow genetic diversity, seventeen SSRs detected polymorphism to distinguish 99 of the 130 accessions. The cluster analysis generated nine clusters and 18 outliers. Genetic diversity within Japanese edamame was lower than that within Chinese vegetable soybean accessions (maodou), even though only 10 Chinese maodou were analyzed compared to 107 Japanese edamame. Cluster analysis revealed that the patterns of SSR diversity in edamame can generally distinguish maturity classes and testa color. We concluded that Japanese edamame have a narrow genetic base different from others and that SSRs can describe the patterns of genetic diversity among the elite vegetable soybean.  相似文献   

A case of lobar emphysema in a dog.     

H Orima  M Fujita  S Aoki  M Washizu  T Yamagami  M Umeda  M Sugiyama 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(4):797-798

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Cross-reactivity among infectious bronchitis viruses in enzyme-linked immunosorbent assay     

H Nagano  T Hohdatsu  M Tsuchimoto  S Ide  T Yamagami  H Yamagishi  Y Fujisaki 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(3):491-497

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Intramolecular carbon isotope distribution of acetic acid in vinegar     

Hattori R  Yamada K  Kikuchi M  Hirano S  Yoshida N 《Journal of agricultural and food chemistry》2011,59(17):9049-9053

Compound-specific carbon isotope analysis of acetic acid is useful for origin discrimination and quality control of vinegar. Intramolecular carbon isotope distributions, which are each carbon isotope ratios of the methyl and carboxyl carbons in the acetic acid molecule, may be required to obtain more detailed information to discriminate such origin. In this study, improved gas chromatography-pyrolysis-gas chromatography-combustion-isotope ratio mass spectrometry (GC-Py-GC-C-IRMS) combined with headspace solid-phase microextraction (HS-SPME) was used to measure the intramolecular carbon isotope distributions of acetic acid in 14 Japanese vinegars. The results demonstrated that the methyl carbons of acetic acid molecules in vinegars produced from plants were mostly isotopically depleted in (13)C relative to the carboxyl carbon. Moreover, isotopic differences (δ(13)C(carboxyl) - δ(13)C(methyl)) had a wide range from -0.3 to 18.2‰, and these values differed among botanical origins, C3, C4, and CAM plants.  相似文献