Infectivity of a novel type of Cryptosporidium andersoni to laboratory mice   总被引：3，自引：0，他引：3  

Matsubayashi M  Kimata I  Iseki M  Hajiri T  Tani H  Sasai K  Baba E 《Veterinary parasitology》2005,129(1-2):165-168

Previously, we reported 'a novel type' of Cryptosporidium andersoni detected from cattle in Japan, and showed that the isolate was infective to mice. In the present study, we examined the patterns of oocyst shedding in both immunocompromised and immunocompetent mice, as well as pathological lesions in the infected mice. After oral inoculation with 1 x 10(6) oocysts, all five severe combined immunodeficiency (SCID) mice began to shed endogenously produced oocysts on day 6 post-inoculation (p.i.). The number of oocysts per day (OPD) reached 1 x 10(6) on day 17 p.i., and an OPD level of 1 x 10(6) to 10(7) was maintained until 91 days p.i. when the mice were sacrificed. In the five immunocompetent mice inoculated with 1 x 10(6) oocysts, the pre-patent and patent periods were 6 and 19 days, respectively, and the maximal OPD level was 1.5 x 10(5) on average. On histological examinations of infected SCID mice, a large number of parasites were present on the surface of the gastric glands of the stomach, but not in other organs examined. In conclusion, the novel type of C. andersoni, which genetically coincides with C. andersoni reported in other countries, is infective to mice, but susceptibility was lower than that of Cryptosporidium muris infecting rodents from the perspective of infectivity to immunocompetent mice.  相似文献   

Immunohistochemical localization of steroidogenic enzymes in corpus luteum of wild sika deer during early mating season     

Matsuura Y  Suzuki M  Hayakawa D  Asano M  Sasaki M  Kitamura N  Yamada J  Tsubota T  Ohtaishi N 《The Japanese journal of veterinary research》2004,51(3-4):167-172

We analyzed the localization of steroidogenic enzymes (P450 scc, 3 beta HSD, P450 arom and P450 c17) in the corpora lutea of two Hokkaido sika deer (Cervus nippon yesoensis) during the early mating season. Two corpora lutea were found in each female and the timing of formation of the corpora lutea seemed different. P450 scc, and 3 beta HSD, positive luteal cells were found in both corpora lutea. The existence of two functional corpora lutea from the early mating season through pregnancy suggests that progesterone secreted by two or more corpora lutea is necessary for maintenance of pregnancy in sika deer.  相似文献   

A simple calculation for obtaining shunt fractions of portosystemic shunts     

Washizu M  Torisu S  Kondo Y  Shimizu N  Washizu T  Takemura N  Kinoshita G 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(4):449-451

Since the isotopes can not be utilized for veterinary patients in Japan, the authors developed a simple calculation formula of shunt fraction of portosystemic shunt based on the hepatic circulation model. The shunt fraction can be calculated utilizing only 2 portal pressure measurements of pre-shunt ligation and temporary or permanent shunt ligation. The calculated shunt fraction can obtained pre-ligation and post-ligation either temporally or permanent complete shunt ligation: complete ligation group of PSS (n=59) had 48.2 +/- 16.9% of shunt fractions, whereas the partial ligation group (n=48) had 71.6 +/- 10.7% of shunt fractions.  相似文献   

B-cell immunoblastic lymphoma with multinucleated giant cells in a cat     

Ohshima M  Morita T  Sawada M  Shimada A  Kobayashi H  Sawashima K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(2):189-191

A 10-year-old male mixed breed cat died after six months history of intermittent vomiting and weight loss. At necropsy, large white-colored foci were found in both kidneys, and whitish thickening of the gastric wall was present at the pyloric part of the stomach. Histopathological examination revealed that both lesions consisted of proliferation of large-sized neoplastic lymphocytes intermingled with multinucleated giant cells. Immunohistochemically, the neoplastic cells were positive for both B-cell antigen receptor complex (CD 79 alpha cy) and MHC class II, although multinucleated giant cells were negative. The present case was diagnosed as B-cell immunoblastic lymphoma with multinucleated giant cells.  相似文献   

Mass and local topography measurements of Itokawa by Hayabusa     

Abe S  Mukai T  Hirata N  Barnouin-Jha OS  Cheng AF  Demura H  Gaskell RW  Hashimoto T  Hiraoka K  Honda T  Kubota T  Matsuoka M  Mizuno T  Nakamura R  Scheeres DJ  Yoshikawa M 《Science (New York, N.Y.)》2006,312(5778):1344-1347

The ranging instrument aboard the Hayabusa spacecraft measured the surface topography of asteroid 25143 Itokawa and its mass. A typical rough area is similar in roughness to debris located on the interior wall of a large crater on asteroid 433 Eros, which suggests a surface structure on Itokawa similar to crater ejecta on Eros. The mass of Itokawa was estimated as (3.58 +/- 0.18) x 10(10) kilograms, implying a bulk density of (1.95 +/- 0.14) grams per cubic centimeter for a volume of (1.84 +/- 0.09) x 10(7) cubic meters and a bulk porosity of approximately 40%, which is similar to that of angular sands, when assuming an LL (low iron chondritic) meteorite composition. Combined with surface observations, these data indicate that Itokawa is the first subkilometer-sized small asteroid showing a rubble-pile body rather than a solid monolithic asteroid.  相似文献   

Establishment and biological characterization of canine histiocytic sarcoma cell lines     

Azakami D  Bonkobara M  Washizu T  Iida A  Kondo M  Kato R  Niikura Y  Iwaki S  Tamahara S  Matsuki N  Ono K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(12):1343-1346

Seven novel cell lines from canine histiocytic sarcoma (HS), three of which were disseminated cutaneous HS and four of which were synovial HS, were established. All of the established cell lines had the same morphological (by light and electron microscopic findings), cytochemical (alpha-naphthyl butyrate esterase-positive), and immunohistochemical (vimentin- and lysozyme-positive, and cyto-keratin-negative) characteristics as the original HS tumor cells. All of the established cell lines injected into nude mice subcutaneously produced solid tumors. Because the established cell lines also showed phagocytic and processing activities, the HS tumor cells appear to originate from the mononuclear phagocytic system cells, despite their differences in locations or organs.  相似文献   

Hemophilia B in a crossbred Maltese dog     

Nakata M  Sakai M  Sakai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1223-1224

A crossbred Maltese dog, 6-year-old, male, was presented to us for examination due to coagulopathy. On examination of blood coagulation screening tests, activated partial thromboplastin time (APTT) was markedly prolonged (63.6 sec). Therefore, a defect in the intrinsic pathway of coagulation was suspected. An additional serum test was also examined and APTT was returned to within the normal range. Furthermore, factor IX coagulation activity was markedly low (2.3%). On the basis of these results, the dog was diagnosed with hemophilia B. The dog has since been presented to us because of hemorrhage problems again after 5, 10, and 16 months, but blood transfusions have maintained good control of its coagulopathy for more than two years.  相似文献   

Identification of Facklamia sourekii from a lactating cow     

Takamatsu D  Ide H  Osaki M  Sekizaki T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1225-1227

A gram-positive, catalase-negative, facultatively anaerobic coccus was isolated from a lactating cow with hematuria and urodynia in Japan. The isolate was identified by 16S rRNA gene sequence analysis as Facklamia sourekii. The biochemical and culture characteristics of the isolate were well consistent with those of F. sourekii type strain. Since all F. sourekii strains reported so far were isolated from human clinical specimens, this is the first reported case of F. sourekii isolated from veterinary clinical specimen.  相似文献   

Generation of gut-homing IgA-secreting B cells by intestinal dendritic cells     

Mora JR  Iwata M  Eksteen B  Song SY  Junt T  Senman B  Otipoby KL  Yokota A  Takeuchi H  Ricciardi-Castagnoli P  Rajewsky K  Adams DH  von Andrian UH 《Science (New York, N.Y.)》2006,314(5802):1157-1160

Normal intestinal mucosa contains abundant immunoglobulin A (IgA)-secreting cells, which are generated from B cells in gut-associated lymphoid tissues (GALT). We show that dendritic cells (DC) from GALT induce T cell-independent expression of IgA and gut-homing receptors on B cells. GALT-DC-derived retinoic acid (RA) alone conferred gut tropism but could not promote IgA secretion. However, RA potently synergized with GALT-DC-derived interleukin-6 (IL-6) or IL-5 to induce IgA secretion. Consequently, mice deficient in the RA precursor vitamin A lacked IgA-secreting cells in the small intestine. Thus, GALT-DC shape mucosal immunity by modulating B cell migration and effector activity through synergistically acting mediators.  相似文献   

Fluorous nanodroplets structurally confined in an organopalladium sphere     

Sato S  Iida J  Suzuki K  Kawano M  Ozeki T  Fujita M 《Science (New York, N.Y.)》2006,313(5791):1273-1276

The distinct properties of fluorous phases are practically useful for separation, purification, and reaction control in organic synthesis. Here, we report the formation of a liquid-like fluorous droplet, composed of 24 perfluoroalkyl chains confined in the interior of a 5-nanometer-sized, roughly spherical shell that spontaneously assembled in solution from 12 palladium ions and 24 bridging ligands. Crystallographic analysis confirmed the rigid shell framework and amorphous interior. Perfluoroalkanes can dissolve in this well-defined fluorous phase, whereas they can hardly dissolve in a surrounding polar organic solution, and their solubility (up to approximately eight perfluoroalkane molecules per spherical complex) can be finely controlled by tuning the length of perfluoroalkyl chains tethered to the shell.  相似文献