Roraimine: a bisbenzylisoquinoline alkaloid from Cissampelos sympodialis roots     

de Lira GA  de Andrade LM  Florêncio KC  da Silva MS  Barbosa-Filho JM  Leitão da-Cunha EV 《Fitoterapia》2002,73(4):356-358

The roots of Cissampelos sympodialis yielded Roraimine (1), a novel bisbenzylisoquinoline alkaloid and liriodenine (2), a known oxoaporphine alkaloid.  相似文献   

Closed-loop control of epilepsy by transcranial electrical stimulation     

A Berényi  M Belluscio  D Mao  G Buzsáki 《Science (New York, N.Y.)》2012,337(6095):735-737

Many neurological and psychiatric diseases are associated with clinically detectable, altered brain dynamics. The aberrant brain activity, in principle, can be restored through electrical stimulation. In epilepsies, abnormal patterns emerge intermittently, and therefore, a closed-loop feedback brain control that leaves other aspects of brain functions unaffected is desirable. Here, we demonstrate that seizure-triggered, feedback transcranial electrical stimulation (TES) can dramatically reduce spike-and-wave episodes in a rodent model of generalized epilepsy. Closed-loop TES can be an effective clinical tool to reduce pathological brain patterns in drug-resistant patients.  相似文献   

Root biomass response to foliar application of imazapyr for two imidazolinone tolerant alleles of sunflower (Helianthus annuus L.)     

Carlos A. Sala  Mariano Bulos  Emiliano Altieri  María Laura Ramos 《Breeding Science》2012,62(3):235-240

Imisun and CLPlus are two imidazolinone tolerance traits in sunflower (Helianthus annuus L.) determined by the expression of two alleles at the locus Ahasl1. Both traits differed in their tolerance level to imazapyr —a type of imidazolinone herbicide— when aboveground biomass is considered, but the concomitant herbicide effect over the root system has not been reported. The objective of this work was to quantify the root biomass response to increased doses of imazapyr in susceptible (ahasl1/ahasl1), Imisun (Ahasl1-1/Ahasl1-1) and CLPlus (Ahasl1-3/Ahasl1-3) homozygous sunflower genotypes. These materials were sprayed at the V2–V4 stage with increased doses of imazapyr (from 0 to 480 g active ingredient ha⁻¹) and 14 days after treatment root biomass of each plant was assessed. Genotype at the Ahasl1 locus, dose of imazapyr and their interaction significantly contributed (P < 0.001) to explain the reduction in root biomass accumulation after herbicide application. Estimated dose of imazapyr required to reduce root biomass accumulation by fifty percent (GR₅₀) differed statistically for the three genotypes under study (P < 0.001). CLPlus genotypes showed the highest values of GR₅₀, 300 times higher on average than the susceptible genotypes, and almost 8 times higher than Imisun materials, demonstrating that both alleles differ in their root biomass response to foliar application of increased doses of imazapyr.  相似文献   

Accuracy assessment and screening of a dairy herd with paratuberculosis by three different ELISAs     

Costanzo G  Pinedo FA  Mon ML  Viale M  Gil A  Illia MC  Gioffré A  Arese A  Travería G  Romano MI 《Veterinary microbiology》2012,156(1-2):183-188

Although the culture of Mycobacterium avium subsp. paratuberculosisis is the gold standard for the diagnosis of paratuberculosis, this bacterium is difficult to grow. In contrast, serological tests like ELISAs are inexpensive, rapid, and easy to perform. The aims of this study were to evaluate the accuracy of three different ELISAs: one with the commercial antigen PPA-3, another one with L5P (a recently described lipopentapeptide), and a third one with an in-house antigen whole cell lysates (WCL) of M. avium (MAA) strain D4ER (Study 1), and to compare them with other tests for paratuberculosis (PTB) diagnosis (Study 2). In Study 1, the sensitivities of the three ELISAs tested were 74.1%, 37% and 74.1%, respectively, whereas their specificities were 98.9%, 100% and 100%, respectively. In Study 2, we compared the three above-mentioned ELISAs with the intradermal reaction test using Avian PPD (PPDa) and fecal culture associated with Ziehl-Neelsen stain and PCR tests, in a dairy herd with 4.6% of cows with clinical signs of PTB. The results showed that fecal samples from 14 cows (16%) were culture-positive and that fecal samples from nine cows (10%) were PPDa-positive. Most of these animals (culture-positive and PPDa-positive) were detected as positive with any of the three ELISAs tested. Serological results showed that 31% of the animals were positive to ELISA-PPA-3, 17% to ELISA-L5P and 42.5% to ELISA-WCL. The combination of these three ELISAs identified 50.6% of the animals as positive in the infected herd. In particular, the results show that the locally developed ELISA seems to be useful for identifying many infected animals in a herd.  相似文献   

Multiplex Nested Reverse Transcription-Polymerase Chain Reaction in a Single Tube for Sensitive and Simultaneous Detection of Four RNA Viruses and Pseudomonas savastanoi pv. savastanoi in Olive Trees     

Bertolini E  Olmos A  López MM  Cambra M 《Phytopathology》2003,93(3):286-292

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The feline dorsal nerve of the penis arises from the deep perineal nerve and not the sensory afferent branch     

Mariano TY  Boger AS  Gustafson KJ 《Anatomia, histologia, embryologia》2008,37(3):166-168

The cat has been used extensively as an animal model for urogenital studies involving the pudendal nerve. However, discrepancies persist in the literature regarding the origin of the dorsal nerve of the penis (DNP). This study used gross dissections and serial histological cross sections to demonstrate that the DNP arises from the deep perineal nerve and not the sensory afferent branch as previously reported. This finding indicates a better than previously appreciated neuroanatomical homology between the cat and human.  相似文献   

Effect of Ahasl1‐1 and Ahasl1‐4 alleles on herbicide resistance and its associated dominance in sunflower     

Gabriela Breccia  Laura Gianotto  Emiliano Altieri  Mariano Bulos  Graciela Nestares 《Pest management science》2019,75(4):935-941

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Immunomodulatory and Antitumoral Activity of Gold Nanoparticles Synthesized by Red Algae Aqueous Extracts     

Noelia Gonzlez-Ballesteros  Lara Diego-Gonzlez  Mariano Lastra-Valdor  Maria Grimaldi  Antonella Cavazza  Franca Bigi  M. Carmen Rodríguez-Argüelles  Rosana Simn-Vzquez 《Marine drugs》2022,20(3)

This study reports on the green and cost-efficient synthesis of gold nanoparticles from three different red algae extracts. The nanoparticles synthesized were fully characterized by UV-Vis spectroscopy, HRTEM, and Z-potential. Relevant components occurring in the extracts, such as polysaccharides or phenolic content, were assessed by analytical techniques such as spectrophotometric assays and liquid chromatography. Finally, the antioxidant, antitumoral, and anti-inflammatory potential of both the extracts and the gold nanoparticles synthesized were analyzed in order to determine a possible synergistic effect on the nanoparticles. The results obtained confirmed the obtainment of gold nanoparticles with significant potential as immunotherapeutic agents. The therapeutic potential of these nanoparticles could be higher than that of inert gold nanoparticles loaded with bioactive molecules since the former would allow for higher accumulation into the targeted tissue.  相似文献   

Iron emission lines from extended x-ray jets in SS 433: reheating of atomic nuclei     

Migliari S  Fender R  Méndez M 《Science (New York, N.Y.)》2002,297(5587):1673-1676

Powerful relativistic jets are among the most ubiquitous and energetic observational consequences of accretion around supermassive black holes in active galactic nuclei and neutron stars and stellar-mass black holes in x-ray binary (XRB) systems. But despite more than three decades of study, the structure and composition of these jets remain unknown. Here we present spatially resolved x-ray spectroscopy of arc second-scale x-ray jets from XRB SS 433 analyzed with the Chandra advanced charge-coupled device imaging spectrometer. These observations reveal evidence for a hot continuum and Doppler-shifted iron emission lines from spatially resolved regions. Apparently, in situ reheating of the baryonic component of the jets takes place in a flow that moves with relativistic bulk velocity even more than 100 days after launch from the binary core.  相似文献   

Dissection of white matter association fasciculi in bovine (Bos taurus), pig (Sus scrofa domesticus) and rabbit (Oryctolagus cuniculus) brains     

Marco Guerrero  Carlos Veuthey  Mariano del Sol  Nicolas E. Ottone 《Anatomia, histologia, embryologia》2020,49(4):550-562

The cerebral fasciculi (association, commissural and projection) pass through the cerebral white matter in organized groups connecting regions, hemispheres, gyri, areas and brain lobes to each other. The study can be done in vivo through diffusion tensor imaging (DTI) but presenting some technical problems. The post-mortem study by dissection allows to have a clearer view of its location, path and connections. In this work, we dissect, identify and compare the fasciculi of association of the white matter of the dorsolateral face of bovine hemispheres (Bos taurus), pig hemispheres (Sus scrofa domesticus) and rabbit hemispheres (Oryctolagus cuniculus), applying the Klingler´s technique. In 30 cerebral hemispheres (10 of each species, five right and five left), we applied the Klingler technique to identify and isolate the occipitofrontal fasciculus, uncinate fasciculus, inferior longitudinal fasciculus and superior longitudinal fasciculus; we established its location by fixing landmarks, and determined the difference in its length and width between the right and left hemispheres as well as between species using the statistical tests of t-student and one-way ANOVA. We identify the gyri, sulci and fasciculi of the dorsolateral surface of the cerebral hemispheres of the three species. We found statistically significant differences in several fasciculi between species principally in the occipitofrontal fasciculus. The preparation of the brains through the modified Klingler technique allowed a successful identification of the fascicules of association of the dorsolateral face of the cerebral hemispheres and the empowerment of these animal models for future research work in this field.  相似文献