Determination of the secondary structure of Kluyveromyces lactis beta-galactosidase by circular dichroism and its structure-activity relationship as a function of the pH     

Tello-Solís SR  Jiménez-Guzmán J  Sarabia-Leos C  Gómez-Ruíz L  Cruz-Guerrero AE  Rodríguez-Serrano GM  García-Garibay M 《Journal of agricultural and food chemistry》2005,53(26):10200-10204

The secondary structure of Kluyveromyces lactis beta-galactosidase was determined by circular dichroism. It is mainly a beta-type protein, having 22% beta-turns, 14% parallel beta-sheet, 25% antiparallel beta-sheet, 34% unordered structure, and only 5% alpha-helix. The structure-activity relationship as a function of the pH was also studied. The pH conditions leading to the highest secondary structure content (100% ellipticity) of the enzyme was found at pH 7.0; at pH 6.5-7.0, the percent ellipticity decreased slightly, suggesting little structural change, but the activity decreased significantly, probably because of variations in critical residues. On the other hand, at pH's above 7.0, a more noticeable change in ellipticity was observed due to structural changes; the CD analysis showed a small increase in the helical content toward higher pH, whereas the maximum activity was found at pH 7.5, meaning that the changes produced in the secondary structure at this pH favored the interaction between the enzyme and the substrate.  相似文献   

Quantification and Characterization of Food Processing Wastes/Residues     

Rolando A. Flores  Carol W. Shanklin  Mariano Loza-Garay  Seung H. Wie 《Compost science & utilization》2013,21(1):63-71

A survey was undertaken to determine the wastes/residues generated by the food processing industry in the state of Iowa by sending a questionnaire to food processors with more than ten employees. Food processors were asked to provide a company profile, information about food waste/residue generated, and information about current disposal methods. Samples of wastes/residues were obtained from food processors willing to provide them, and proximate analysis was done. The overall response rate was 82.2 percent or 365 respondents out of 444 questioned. Companies under different category of standard industrial code (SIC) could produce similar wastes/residues that were classified in the same waste/residue streams. Within the meat and seafood category, the majority (87.7 percent) of the wastes/residues were generated by SIC codes 2077 (animal, marine fats and oils) and 2015 (poultry slaughtering, dressing, and processing). Among the waste management practices reported, excluding “other,” land application accounted for 14.0 percent of the waste stream, followed by rendering with 11.4 percent, landfill with 6.7 percent, sewage on-site with 5.0 percent and animal feed production off-site with 4.3 percent. Other waste management practices (combinations of major practices or others not clearly defined) account for 55.1 percent of the allocation of the waste stream.  相似文献   

Selection of watermelon genotypes for resistance to bacterial fruit blotch     

Francisco C. Q. Carvalho  Liliana A. Santos  Rita C. S. Dias  Rosa L. R. Mariano  Elineide B. Souza 《Euphytica》2013,190(2):169-180

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Benefits under the Sea: The Role of Marine Compounds in Neurodegenerative Disorders     

Mariano Catanesi  Giulia Caioni  Vanessa Castelli  Elisabetta Benedetti  Michele d&#x;Angelo  Annamaria Cimini 《Marine drugs》2021,19(1)

Marine habitats offer a rich reservoir of new bioactive compounds with great pharmaceutical potential; the variety of these molecules is unique, and its production is favored by the chemical and physical conditions of the sea. It is known that marine organisms can synthesize bioactive molecules to survive from atypical environmental conditions, such as oxidative stress, photodynamic damage, and extreme temperature. Recent evidence proposed a beneficial role of these compounds for human health. In particular, xanthines, bryostatin, and 11-dehydrosinulariolide displayed encouraging neuroprotective effects in neurodegenerative disorders. This review will focus on the most promising marine drugs’ neuroprotective potential for neurodegenerative disorders, such as Parkinson’s and Alzheimer’s diseases. We will describe these marine compounds’ potential as adjuvant therapies for neurodegenerative diseases, based on their antioxidant, anti-inflammatory, and anti-apoptotic properties.  相似文献   

Quantitative detection of <Emphasis Type="Italic">Citrus tristeza virus</Emphasis> in plant tissues and single aphids by real-time RT-PCR     

Edson Bertolini  Aranzazu Moreno  Nieves Capote  Antonio Olmos  Ana de Luis  Eduardo Vidal  Jordi Pérez-Panadés  Mariano Cambra 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(2):177-188

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Genomic insights into the immune system of the sea urchin     

Rast JP  Smith LC  Loza-Coll M  Hibino T  Litman GW 《Science (New York, N.Y.)》2006,314(5801):952-956

Comparative analysis of the sea urchin genome has broad implications for the primitive state of deuterostome host defense and the genetic underpinnings of immunity in vertebrates. The sea urchin has an unprecedented complexity of innate immune recognition receptors relative to other animal species yet characterized. These receptor genes include a vast repertoire of 222 Toll-like receptors, a superfamily of more than 200 NACHT domain-leucine-rich repeat proteins (similar to nucleotide-binding and oligomerization domain (NOD) and NALP proteins of vertebrates), and a large family of scavenger receptor cysteine-rich proteins. More typical numbers of genes encode other immune recognition factors. Homologs of important immune and hematopoietic regulators, many of which have previously been identified only from chordates, as well as genes that are critical in adaptive immunity of jawed vertebrates, also are present. The findings serve to underscore the dynamic utilization of receptors and the complexity of immune recognition that may be basal for deuterostomes and predicts features of the ancestral bilaterian form.  相似文献   

Serum concentrations of calcium, phosphorus, magnesium and calciotropic hormones in donkeys     

Lopez I  Estepa JC  Mendoza FJ  Rodriguez M  Aguilera-Tejero E 《American journal of veterinary research》2006,67(8):1333-1336

OBJECTIVE: To provide reference values for serum biochemical variables that are used for evaluation of mineral metabolism in donkeys and compare values with those in horses. ANIMALS: 18 donkeys and 18 horses. PROCEDURES: Total calcium (tCa), total magnesium (tMg), and inorganic phosphorus (P) concentrations were measured in serum samples via spectrophotometry. Ionized calcium (iCa) and magnesium (iMg) concentrations were quantified with selective electrodes. By use of a micropartition system, tCa and tMg were fractionated to separate protein-bound (pCa, pMg) and ultrafiltrable fractions. Complexed calcium (cCa) and magnesium (cMg) concentrations were calculated by substracting ionized fractions from ultrafiltrable fractions. Parathyroid hormone (PTH) and calcitriol (CTR) concentrations were measured via radioimmunoassay. RESULTS: Serum tCa concentration in donkeys (3.37 +/- 0.21 mmol/L) was composed of pCa (1.59 +/- 0.21 mmol/L [47.0 +/- 4.2%]), iCa (1.69 +/- 0.04 mmol/L [50.4 +/- 3.0%]), and cCa (0.09 +/- 0.08 mmol/L [2.6 +/- 2.9%]). Serum tMg concentration (1.00 +/- 0.08 mmol/L) was fractioned in pMg (0.23 +/- 0.08 mmol/L [23.4 +/- 8.1%]), iMg (0.59 +/- 0.04 mmol/L [58.8 +/- 5.1%]), and cMg (0.18 +/- 0.08 mmol/L [17.8 +/- 7.2%]). Serum concentrations of P (1.14 +/- 0.30 mmol/L), PTH (20.4 +/- 21.2 pg/mL), and CTR (13.4 +/- 5.9 pg/mL) were determined. CONCLUSIONS AND CLINICAL RELEVANCE: Serum variables of mineral metabolism in donkeys were within reference ranges for horses. However, when compared with horses, donkeys had higher iCa, cMg, and CTR and lower pMg and PTH concentrations.  相似文献   

Computed tomography measurements of thoracic structures in 26 clinically normal goats     

Ohlerth S  Becker-Birck M  Augsburger H  Jud R  Makara M  Braun U 《Research in veterinary science》2012,93(1):7-12

There is increasing evidence that activation of innate immunity, in animals and man, by live vaccines, sub-unit vaccines or synthetic or non-synthetic stimulants can induce a profound and rapidly induced resistance to pathogens, including infectious agents that are unrelated to the stimulating antigen or agent. We review the evidence for this phenomenon and present the proposition that this approach might be used to stimulate immunity during the life of the animal when susceptibility to infection is high and when normal vaccination procedures may be inappropriate.  相似文献   

Can a natural experiment be used as a tool to design partial cutting regimes? The decline of <Emphasis Type="Italic">Austrocedrus chilensis</Emphasis> forests,an example     

Mariano M. Amoroso  Bruce C. Larson 《Journal of Forest Research》2010,15(1):38-45

The forests of Austrocedrus chilensis in Argentina suffer mortality from what is locally known as ‘mal del ciprés’. This disturbance usually appears as aggregations of dead trees of varying sizes. The mortality process seems unpredictable, and regeneration establishment is variable under these conditions. The highly variable mortality rates results in a natural experiment that allows the correlation of overstory mortality with regeneration rate. This correlation should then make it possible to estimate the regeneration success which would follow after different levels of partial cutting, allowing the purposeful direction of the regeneration process. We collected data from eight Austrocedrus chilensis-dominated stands and reconstructed overstory mortality over time and correlated it to the regeneration recruitment. Mortality showed two patterns: increasing ‘continuous’ mortality over time, and ‘pulses’ of important basal area loss in short periods. Overstory mortality had an effect on the recruitment and composition of the regeneration by allowing different species establishment depending on the degree of mortality. By quantifying mortality basal area and recruitment over time, we calculated estimates that can provide partial cutting guidance. Partial cuttings would then allow the purposeful direction of the regeneration process. Stand reconstruction studies provided a useful tool to help designing regeneration systems using a natural experiment. Some specific management implications for symptomatic A. chilensis forests are given.  相似文献   

Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation   总被引：1，自引：0，他引：1  

Sandra Branco  Jo?o Orvalho  Alexandre Leit?o  Isadora Pereira  Manuel Malta  Isabel Mariano  Tania Carvalho  Rui Baptista  Brian R. Shiels  Maria C. Peleteiro 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(1):27-34

This study was carried out to investigate fifteen cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria (T.) annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and T. annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscles, pharynx, trachea and intestinal serosa. Histologically, nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79αcy) labeled very few cells. T. annulata infected cells in these nodules were also identified by IHC through the use of two monoclonal antibodies (1C7 and 1C12) which are diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes.  相似文献