Polycystic kidney disease: a review and occurrence in Slovenia with comparison between ultrasound and genetic testing     

Domanjko-Petric A  Cernec D  Cotman M 《Journal of Feline Medicine and Surgery》2008,10(2):115-119

Polycystic kidney disease (PKD) is an inherited autosomal kidney disease which is most commonly identified in Persian and Persian related cats. Positive cats have multiple cysts of various sizes that occur in the renal cortex and medulla and occasionally in other abdominal organs. PKD often leads to renal failure which occurs from mid to late in life. Renal cysts can be diagnosed ultrasonographically after 7 weeks of age by an experienced ultrasonographer and a high resolution machine. However, ultrasonography is now being replaced by genetic screening. A total of 340 cats of variable breeds aged from 5 months to 18 years were ultrasonographically examined in the past 7 years at the University Veterinary Small Animal Clinic. Of these, 13.8% were PKD positive with very high prevalence in Persian cats (36%). There was no sex predilection identified. The C>A transversion at position 3284 on exon 29 of PKD1 gene, resulting in a stop mutation has been identified in the heterozygous state in eight affected cats examined (Persian breed). All heterozygous cats were also ultrasonographically positive.  相似文献   

Quality of Wine Grape Cultivars Grown in Czech Republic     

Martin Král  Martina Ošťádalová  Jana Pokorná  Bohuslava Tremlová  Eliška Rohrerová  Marek Šnirc  Tomáš Fekete 《Erwerbs-Obstbau》2018,60(1):29-35

This research aims to make an analysis of significant parameters in wine grape from Czech Republic. It was evaluated pH, content of sugar, ascorbic acid, titratable acidity, total phenolic content and texture properties. For analyzes 5 samples of wine grape were used, harvested during the three periods. The results show that during the ripening of wine grapes there were changes of selected qualitative parameters, particularly of total polyphenols, sugar and some varieties of wine grape also ascorbic acid (significantly in the variety ’Grüner Veltliner’, ’Chardonnay’, ’Pinot Blanc’). Pronounced changes qualitative parameters were among wine grapes harvested the 1st and 2nd seasons. Changes in textural properties also occurred during the harvest and for most varieties between 2nd and 3rd period of harvest. The PCA grouped chemical parameters of wine grape in independent sets and can be used as assessment tool of wine cultivars.  相似文献   

Impact of quercetin and EGCG on key elements of the Wnt pathway in human colon carcinoma cells     

Pahlke G  Ngiewih Y  Kern M  Jakobs S  Marko D  Eisenbrand G 《Journal of agricultural and food chemistry》2006,54(19):7075-7082

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Antimicrobial activity of Bryum argenteum     

Sabovljevic A  Sokovic M  Sabovljevic M  Grubisic D 《Fitoterapia》2006,77(2):144-145

The antimicrobial activity of Bryum argenteum ethanol extracts was evaluated by microdilution method against four bacterial (Escherichia coli, Bacillus subtilis, Micrococcus luteus and Staphilococcus aureus) and four fungal species (Aspergillus niger, Penicillium ochrochloron, Candida albicans and Trichophyton mentagrophyes). All the investigated ethanol extracts have been proved to be active against all bacteria and fungi tested.  相似文献   

The Influence of Maca (<Emphasis Type="Italic">Lepidium meyenii</Emphasis>) on Antioxidant Status,Lipid and Glucose Metabolism in Rat   总被引：1，自引：0，他引：1  

Vecera R  Orolin J  Skottová N  Kazdová L  Oliyarnik O  Ulrichová J  Simánek V 《Plant foods for human nutrition (Dordrecht, Netherlands)》2007,62(2):59-63

This work focused on the effect of maca on lipid, anti-oxidative, and glucose parameters in hereditary hypertriglyceridemic (HHTg) rat. Maca (1%) was administred to rats as a part of a high-sucrose diet (HSD) for 2 weeks. Rosiglitazone (0.02%) was used as a positive control. Maca significantly decreased the levels of VLDL (very low density lipoproteins), LDL (low density lipoproteins), and total cholesterol, and also the level of TAG (triacylglycerols) in the plasma, VLDL, and liver. Maca, as well as rosiglitazone, significantly improved glucose tolerance, as the decrease of AUC (area under the curve) of glucose showed, and lowered levels of glucose in blood. The activity of SOD (superoxide dismutase) in the liver, the GPX (glutathione peroxidase) in the blood, and the level of GSH (glutathione) in liver increased in all cases significantly. Results demonstrate that maca seems to be promising for a positive influence on chronic human diseases (characterized by atherogenous lipoprotein profile, aggravated antioxidative status, and impaired glucose tolerance), and their prevention.  相似文献   

Effect of Slovenian climatic conditions on the development and survival of the root-knot nematode <Emphasis Type="Italic">Meloidogyne ethiopica</Emphasis>     

Polona Strajnar  Saša Širca  Matej Knapič  Gregor Urek 《European journal of plant pathology / European Foundation for Plant Pathology》2011,129(1):81-88

Meloidogyne ethiopica is a tropical root-knot nematode species which has recently been found in Europe. We examined its ability to survive in open fields located in regions with sub-Mediterranean and continental European climates. The outdoor microplot experiment consisted of two locations and lasted three growing and two winter seasons. It was demonstrated that M. ethiopica was able to survive at both locations and also that it retained its infection ability although temperatures below zero were recorded. The correct species was confirmed after each winter season by isozyme electrophoresis. Furthermore, the influence of temperature on the reproduction cycle of M. ethiopica was investigated. Meloidogyne ethiopica required 67, 48 and 36 days to complete the reproduction cycle at mean daily temperatures of 18.3, 22.7 and 26.3°C, respectively. At 13.9°C, M. ethiopica was not able to reproduce. The data obtained from these experiments were used to develop a correlation between temperature and the time needed for M. ethiopica to complete a reproduction cycle using a mathematical equation. Furthermore, eight vegetable crops that are important for agricultural production in Slovenia were tested for their suitability as hosts for M. ethiopica.  相似文献   

Growth regulation of human melanocytes: mitogenic factors in extracts of melanoma, astrocytoma, and fibroblast cell lines   总被引：5，自引：0，他引：5  

M Eisinger  O Marko  S Ogata  L J Old 《Science (New York, N.Y.)》1985,229(4717):984-986

Melanocytes derived from fetal or adult skin do not propagate in vitro unless cultured in the presence of factors such as 12-O-tetradecanoylphorbol 13-acetate (TPA). In a search for physiological factors regulating the growth of melanocytes, extracts of various cultured cell types were tested. Factors produced by melanoma and astrocytoma cell lines support continued proliferation of melanocytes in the absence of TPA. WI-38, a fibroblast cell line derived from human embryonic lung, was the most active source of melanocyte growth factors. No melanocyte growth-promoting activity was found in extracts of cultured neuroblastoma, renal cancer, normal keratinocytes, or renal epithelium. Nerve growth factor, epidermal growth factor, melanocyte-stimulating hormone, transforming growth factor-beta, and platelet-derived growth factor did not have growth-promoting activity for melanocytes. The presence of melanocyte growth factors and TPA together resulted in the strongest mitogenic activity for melanocytes, permitting the recovery (at 20 days) of 4 to 20 times as many cells as in growth factor or TPA alone.  相似文献   

Biochemical Properties of Red Currant Varieties in Relation to Storage     

Djordjević B  Savikin K  Zdunić G  Janković T  Vulić T  Oparnica C  Radivojević D 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(4):326-332

Eleven red currant varieties (Ribes rubrum L.) cultured in Serbia were evaluated for some of their biochemical properties such as total phenolics, anthocyanins, ascorbic acid, invert sugar, soluble solid content, and acidity. The average amount of ascorbic acid varied from 50.5–71.6 mg/100 g FW, while concentration of invert sugars ranged from 6.0%–9.0%. The highest amounts of total phenolics and anthocyanins were detected in variety Redpoll (153.4 mg GAE/100 g FW and 19.3 mg/100 g, respectively). Red currants were processed to juice, and the phenolic and anthocyanin contents changed as a result of processing. Berries and juices were long-term stored at −18 °C and changes in phytochemicals were monitored. In berries, storage caused the decrease of ascorbic acid content up to 49%, and a general reduction of total phenolics was also noticed. In juices, total phenolics content increased after one year of storage. In both berries and juices total anthocyanins increased during storage by up to 85% and 50%, respectively. This study demonstrates that certain varieties, namely Redpoll, Jonkheer and London Market are good source of phytochemicals, retaining the nutritional value during processing and storage.  相似文献   

Fusaproliferin effects on the photosystem in the cells of maize seedling leaves     

Antonello Santini  Antonia Šrobárová  Ján Pavlovkin  Milada Čiamporová  Alberto Ritieni 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(4):363-371

The possible role of the fusariotoxin, fusaproliferin in plant pathology was investigated with respect to cell membrane potential. Electron microscopy was used to study both the early effect of fusaproliferin on the host’s plasma membrane and ultrastructure responses in the cells of maize leaves. The seedlings of resistant (Lucia) and susceptible (Pavla) to the fusaproliferin maize cultivars were grown in the presence of fusaproliferin at different concentrations, namely 5 and 35 μg ml⁻¹, respectively, and electrophysiological measurements were compared with those obtained using two different toxic compounds, namely fusicoccin and 3-3(3,4 dichlorophenyl)-1,1-dimethylurea (DCMU). It was observed that only the higher concentration of fusaproliferin induced the onset of visible symptoms on the leaves. Comparing the effect of fusaproliferin to that of fusicoccin and DCMU at the higher toxin concentration, it was observed that functional differences in membrane potential induced severe damage to the mesophyll and outer chloroplast membrane; the extent of changes in electrophysiology and ultrastructure disturbances depended on the toxin concentration and was greater in the susceptible cv. Pavla. Results indicated that fusaproliferin could be involved in Fusarium pathogenesis either as a virulence factor or by enhancing the activity of other toxins that might be concomitantly present in infected plants.  相似文献   

Genetic variability of <Emphasis Type="Italic">Plum pox virus</Emphasis> isolates in the Czech Republic     

Sébastien Gadiou  Dana Šafářová  Milan Navrátil 《European journal of plant pathology / European Foundation for Plant Pathology》2008,121(4):513-517

Plum pox virus (PPV), the causal agent of Sharka disease, is an important pathogen of stone fruit trees. In this study, 24 new Czech PPV isolates from five different orchards were collected and characterized, molecularly. PPV-D isolates were identified in all orchards studied; whereas PPV-Rec isolates were identified in only two of them. A phylogenetic analysis on (Cter) NIb-(Nter) CP was performed. Three Czech PPV-D isolates BOH11CZ, BOH12CZ, and BOH13CZ diverged into a significantly separated cluster. PPV-Rec isolates formed a fairly homogenous group. However, the Bohutice and the Lipov PPV-Rec isolates clustered in two significantly separated branches.  相似文献