Interferon-gamma response of PBMC indicates productive pseudorabies virus (PRV) infection in swine   总被引：1，自引：0，他引：1  

Hoegen B  Saalmüller A  Röttgen M  Rziha HJ  Geldermann H  Reiner G  Pfaff E  Büttner M 《Veterinary immunology and immunopathology》2004,102(4):389-397

In Chinese Meishan/German Landrace cross-bred swine F2 generation interferon gamma (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) was determined directly ex vivo at different time points after survival of a virulent pseudorabies virus (PRV) infection. This reactivity was compared with the reactivity of na?ve PBMC. Significant IFN-gamma production was determined in ELISA and ELISPOT only after in vitro PBMC re-stimulation with PRV and not with the closely related bovine herpesvirus BHV-1. The PRV-specific IFN-gamma secretion from re-stimulated PBMC showed high levels 6 days after infection, before the presence of serum antibodies, and it persisted at a high level over a 3 months period. The response of a group of eight piglets infected intranasally with PRV varied. Only two animals showed the expected typical fever response. PRV specific IFN-gamma production by PBMC clearly indicated that infection had occurred. Early significant IFN-gamma production by primed PBMC turned out to be a reliable and specific ex vivo marker for cellular response against productive PRV infection in swine before antibody formation.  相似文献   

Pathology in practice. Subcutaneous granulomas     

S Gumber  R Schnellbacher  MS Orandle  N Wakamatsu 《Journal of the American Veterinary Medical Association》2012,241(3):327-329

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PALLIATIVE RADIOTHERAPY OF APPENDICULAR OSTEOSARCOMA IN 95 DOGS     

Oscar  Ramirez  III  DVM  Richard K.  Dodge  MS  Rodney L.  Page  DVM  MS  G. Sylvester  Price  DVM  PhD  Marlene L.  Hauck  DVM  PhD  Tracy A.  Ladue  DVM Felicia  Nutter  DVM  Donald E.  Thrall  DVM  PhD 《Veterinary radiology & ultrasound》1999,40(5):517-522

Ninety-five dogs with either a presumptive (n = 24) or biopsy confirmed diagnosis (n = 71) of osteosarcoma received palliative radiotherapy using 60Co photons. Parallel opposed beams were used with each dog receiving either 10 Gy on days 0, 7 and 21 (n = 58) or 8 Gy on days 0 and 7 (n = 37). The 8 Gy fractionation scheme was given with the intent of retreating upon relapse from pain relief. Only 9 of 37 (24%) dogs in the 8 Gy group returned for retreatment. Forty-seven of the 95 dogs (49%) received concurrent or sequential chemotherapy. Seventy of the 95 dogs (74%) experienced pain relief following treatment. In dogs experiencing pain relief the median duration of response was 73 days. Numerous clinical variables were evaluated as predictors of response. The only variable significantly related to achieving a response was the use of chemotherapy. The following variables were significantly related to the duration of response: extent of bone lysis, chemotherapy use, length of bone involved and tumor site (humerus). In a multivariate analysis (n = 73 dogs), after adjusting for chemotherapy use, extent of bone involvement (p = 0.01) and tumor site (p = 0.02) retained statistical significance, while degree of bone lysis did not (p = 0.11). No difference in response incidence or duration was found between 3 fractions of 10 Gy vs. 2 fractions of 8 Gy. Administration of a low initial dose with the intent of retreatment was not a successful strategy.  相似文献   

Enhanced resistance to bacterial infection byErwinia carotovora subsp.atroseptica in transgenic potato plants expressing the attacin or the cecropin SB-37 genes     

Patricio Arce  Mauricio Moreno  Mónica Gutierrez  Marlene Gebauer  Paola Dell’Orto  Hebert Torres  Ivette Acuña  Pauline Oliger  Alejandro Venegas  Xavier Jordana  Julio Kalazich  Loreto Holuigue 《American Journal of Potato Research》1999,76(3):169-177

Blackleg and soft rot diseases, caused by the bacteriumErwinia carotovora, are among the diseases that cause important losses in culture and storage of potato. In this paper, we introduced bacterial resistance into potato, via genes encoding for proteins with antibacterial activity. For this purpose, potato clones were transformed either with the gene encoding the acidic attacin protein fromHyalophora cecropia, or with the gene encoding the cecropin analog peptide SB37. These clones were evaluated for soft rot and blackleg resistance, after inoculation with the bacterial strainErwinia carotovora subsp.atroseptica T7. Results reported in this paper indicate that a considerable percentage of the potato clones (15–22%) showed increased resistance to bacterial infection, revealed by reduced severity of blackleg or soft rot symptoms. Expression of the transgenes was demonstrated in some of the clones by Northern blot analysis. This is the first report indicating that expression of the gene encoding for an attacin protein and for the cecropin SB-37 peptide in transgenic potato confers increased resistance to bacterial infection.  相似文献   

Antioxidant and anti‐inflammatory activities of Pinus radiata bark extract in salmonid cell lines          下载免费PDF全文

Allisson Astuya  Javiera Ziehe  Alejandra Rivera  Sebastián Ortiz  Viviana Ulloa  Marlene Roeckel  Estrella Aspé  Katherina Fernández 《Aquaculture Research》2017,48(7):3568-3578

A fish meal supply shortage is limiting aquaculture development. Currently, plant‐based proteins, such as soya bean meal, are being used as an alternative protein source, despite that such a diet can adversely affect fish, such as by inducing an inflammatory response. A possible solution is to include dietary additives in farm diets to counteract negative effects. One such solution originates from pine bark extracts, which present bioactive properties. In this study, the antioxidant and anti‐inflammatory properties of Pinus radiata bark extracts were evaluated for the first time in a salmonid cell line. This extract chemically demonstrated antioxidant activity through 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH = 58.4 ± 1.1%) and ferric ion reducing antioxidant power (FRAP = 575 ± 17 mgEqFe(II)·g extract^?1) assays. Additionally, the extract showed high flavonoid and phenolic compound contents. Up to 100 mg mL^?1, the P. radiata extract showed no cytotoxicity in the CHSE‐214 salmonid embryo cell line. Moreover, the antioxidant activity of the extract (50 μg mL^?1) was evaluated by a dichlorofluorescein (DCFH) assay in the SHK‐1 salmon cell line challenged with an oxidant stimulus (H₂O₂), showing 58.9% activity. The extract also protected DNA from oxidative damage, as observed through a comet assay. When assessing anti‐inflammatory properties in an in vitro inflammation model, the extract significantly reduced the relative expression of the pro‐inflammatory cytokines interleukin‐1β (IL‐1β), tumour necrosis factor‐α (TNF‐α) and interleukin‐8 (IL‐8) and of the inducible cyclooxygenase‐2 (COX‐2) enzyme. These results suggest a potential application of P. radiata bark extract in functional foods in aquaculture.  相似文献   

Ridge to reef modelling for use within land–sea planning under data‐limited conditions          下载免费PDF全文

Jeremy Rude  Andrew Minks  Brandon Doheny  Marlene Tyner  Katy Maher  Christine Huffard  Nur Ismu Hidayat  Hedley Grantham 《水产资源保护:海洋与淡水生态系统》2016,26(2):251-264

Oocysts of Hepatozoon canis in Rhipicephalus (Boophilus) microplus collected from a naturally infected dog     

de Miranda RL  de Castro JR  Olegário MM  Beletti ME  Mundim AV  O'Dwyer LH  Eyal O  Talmi-Frank D  Cury MC  Baneth G 《Veterinary parasitology》2011,177(3-4):392-396

Canine hepatozoonosis is a tick-borne disease caused by protozoans of the genus Hepatozoon. Several tick species have been implicated as potential vectors. Therefore, extensive studies are needed to determine the 'natural' endemic cycle of this parasite. This paper presents the first report of the presence of Hepatozoon canis oocysts in Rhipicephalus (Boophilus) microplus collected from an infected dog.  相似文献   

Purification and characterization of two novel beta-galactosidases from Lactobacillus reuteri     

Nguyen TH  Splechtna B  Steinböck M  Kneifel W  Lettner HP  Kulbe KD  Haltrich D 《Journal of agricultural and food chemistry》2006,54(14):4989-4998

The intracellular beta-galactosidase (beta-gal) enzymes from two strains of Lactobacillus reuteri, L103 and L461, were purified by ammonium sulfate fractionation, hydrophobic interaction, and affinity chromatography. Both enzymes are heterodimers with a molecular mass of 105 kDa, consisting of a 35 kDa subunit and a 72 kDa subunit. Active staining of L. reuteri L103 and L461 beta-gal with 4-methylumbelliferyl beta-d-galactoside showed that the intact enzymes as well as the larger subunits possess beta-galactosidase activity. The isoelectric points of L. reuteri L461 and L103 beta-gal were found to be in the range of 3.8-4.0 and 4.6-4.8, respectively. Both enzymes are most active in the pH range of 6-8; however, they are not stable at pH 8. The L. reuteri beta-galactosidases are activated by various mono- and divalent cations, including Na(+), K(+), and Mn(2+), and are moderately inhibited by their reaction products d-glucose and d-galactose. Because of their origin from beneficial and potentially probiotic lactobacilli, these enzymes could be of interest for the synthesis of prebiotic galacto-oligosaccharides.  相似文献   

Prospective clinical evaluation of serum cardiac troponin T in dogs admitted to a veterinary teaching hospital     

DeFrancesco TC  Atkins CE  Keene BW  Coats JR  Hauck ML 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(5):553-557

The purpose of this study was to measure serum cardiac troponin T (cTnT) with a commercially available human enzyme-linked immunoassay (ELISA) test in various groups of dogs, including those undergoing doxorubicin chemotherapy. Serum samples were obtained from 6 groups of dogs: (1) normal adult dogs (n = 15); (2) dogs with asymptomatic dilated cardiomyopathy (n = 5); (3) dogs with congestive heart failure (n = 10); (4) dogs with untreated neoplasia (n = 20); (5) dogs with skeletal muscle trauma (n = 10); and (6) dogs with neoplasia receiving doxorubicin chemotherapy (n = 4). One serum sample was obtained from each of the normal dogs, those with asymptomatic cardiomyopathy, those with congestive heart failure, and those with untreated neoplasia. Serum samples were obtained serially from the dogs that were undergoing doxorubicin chemotherapy; samples were collected before doxorubicin (30 mg/m2) administration and then 1, 5, 7, and 14 days after administration throughout 6 cycles for a cumulative total dose of 180 mg/m2. All normal dogs, dogs with untreated neoplasia, and dogs with asymptomatic dilated cardiomyopathy had cTnT concentrations below the lower limits of detection for the assay used (<0.05 ng/mL). Detectable concentrations of cTnT were found in 3 dogs with congestive heart failure and in 2 dogs with skeletal muscle trauma. Detectable concentrations also were found in both dogs that had received 180 mg/m2 of doxorubicin. We conclude that dogs with congestive heart failure and those with skeletal muscle trauma and dogs with neoplasia receiving high-dose doxorubicin chemotherapy may have increased serum cTnT concentration, which may be suggestive of myocardial damage.  相似文献   

New records of Rhipicephalus linnaei infected by Rickettsia massiliae from Central Mexico     

José Francisco Nieto-Cabrales  Beatriz Salceda-Sánchez  Héctor M. Zazueta-Islas  Marlene Solís-Cortés  Magaly Guadalupe Landa-Flores  Juan Carlos del Mazo-López  Laura Valtierra-Alzaga  Juan J. Soto-Gutiérrez  Heron Huerta-Jimenez  Ingeborg Becker  Jorge J. Rodríguez-Rojas  Sokani Sánchez-Montes 《Zoonoses and public health》2024,71(2):217-224

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