Induction of proinflammatory cytokines and caspase-1 by leptin in monocyte/macrophages from holstein cows   总被引：3，自引：0，他引：3  

Ahmed M  Shaban Z  Yamaji D  Okamatsu-Ogura Y  Soliman M  Abd Eldaim M  Ishioka K  Makondo K  Saito M  Kimura K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):509-514

The proliferation of peripheral blood mononuclear cells (PBMC) containing both monocyte/macrophages and T lymphocytes increased after treatment with T-cell mitogen (concanavalin A: Con A). PBMC treated with either leptin alone or combination of leptin and ConA showed enhanced proliferative activity by 10-40%, compared with those treated with ConA alone. In contrast, isolated T lymphocytes treated with leptin and ConA showed lowered proliferative activity than the ConA-treated alone, indicating that leptin induced production of some cytokines from monocyte/macrophages, that subsequently resulted in enhancement of T lymphocytes proliferation in PBMC. Among the cytokines examined, monocyte/monocytes constitutively expressed interleukin (IL)-1beta, IL-12p35, IL-18 mRNA, and faintly expressed tumor necrosis factor (TNF)-alpha and IL-12p40 mRNA. Leptin treatment augmented the monocyte/macrophages mRNA expression of only TNF-alpha and IL-12p40 to comparable levels of cells treated with lipopolysaccharide (LPS). However, leptin treatment increased monocyte/macrophages production of IL-1beta as well as TNF-alpha, and induced the mRNA expression of caspase-1, which is shown to mediate the conversion of latent pro-IL-1beta and pro-IL-18 to active forms. These results suggest that leptin directly acts on monocyte/macrophages to produce factors that induce T lymphocytes proliferation such as IL-12p35/p40 complex through IL-12p40 induction and IL-1beta/IL-18 production through caspase-1 induction.  相似文献   

Lures for red palm weevil trapping systems: aggregation pheromone and synthetic kairomone          下载免费PDF全文

Sandra Vacas  Ourania Melita  Antonios Michaelakis  Panagiotis Milonas  Roxana Minuz  Paola Riolo  Mohamed Kamal Abbass  Paolo Lo Bue  Stefano Colazza  Ezio Peri  Victoria Soroker  Yaara Livne  Jaime Primo  Vicente Navarro‐Llopis 《Pest management science》2017,73(1):223-231

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Characterization and photodegradation mechanism of three Algerian wood species   总被引：1，自引：0，他引：1  

Yasmina Ouadou  Djamel Aliouche  Marie-France Thevenon  Mohamed Djillali 《Journal of Wood Science》2017,63(3):288-294

Aging is the irreversible change of mechanical, physical, and chemical properties of materials; the main objective of this work was to study the photochemical degradation and structural changes of three major Algerian wood species. For this, we evaluated the photodegradation mechanism for Maritime Pine (Pinus pinaster), zeen oak (Quercus canariensis), and afares oak (Quercus afares) by accelerated aging in a Xenon test chamber. Degradation of the samples was established by an initial color change (after 30 h exposure), followed by roughening and cracking (120 h exposure) as translated by scanning electron microscopy and Fourier transform infrared spectroscopy. The discoloration of irradiated wood samples was primarily related to the decomposition of lignin which is the key structure in wood photodegradation. As expected, a decrease in mechanical properties was observed; for all samples, the modulus of elasticity decreased after aging, indicating that the wood specimens loss some of their stiffness.  相似文献   

Improving productivity and nutrients uptake of wheat plants using Moringa oleifera leaf extract in sandy soil     

Abdel-Rahman M. A. Merwad  Mohamed K. Abdel-Fattah 《Journal of plant nutrition》2017,40(10):1397-1403

A field experiment was conducted on a sandy soil at Salhia El-Gdida, Al Sharqia government, Egypt, to study the effect of Moringa leaf extract on yield and nutrient uptake of wheat plants (Triticum aestivum cv.). Foliar spraying of Moringa leaf extract was done at 40, 70, and 90 days after planting at a rate of 0%, 1%, 2%, 3%, and 4%. Treatments of Moringa extract significantly increased straw and grain yield, biological yield, 1000 grain weight, yield efficiency, protein content, and nutrient uptake as compared to untreated plants in the both seasons. The highest values of straw and grain yield, quality yield, and nutrient uptake by plants were obtained with 4% of Moringa extract, while the lowest values were obtained with untreated plants. Also, the highest percentage increase in grain yield of 71% and 88% was recorded from the treatment 4% of Moringa extract in first and second seasons, respectively.  相似文献   

Salicylic acid improves the antioxidant ability against arsenic-induced oxidative stress in sunflower (Helianthus annuus) seedling     

Issam Saidi  Nasreddine Yousfi  Mohamed Ali Borgi 《Journal of plant nutrition》2017,40(16):2326-2335

The present study investigated the potential role of external salicylic acid (SA) in alleviating Arsenic (As) toxicity in sunflower leaves. The exposure of plants to 10 µM As inhibited biomass production and intensively increased the accumulation of As in both roots and leaves. The levels of some important parameters associated with oxidative stress, namely lipid peroxidation, electrolyte leakage, and hydrogen peroxide (H₂O₂) production were increased. SA application alleviated the negative effect of As on growth and led to decrease in oxidative injuries. Furthermore, SA application led to higher activity of catalase, ascorbate peroxidase, and glutathione peroxidase, and concomitantly decreased superoxide dismutase and guaiacol peroxidase activities. As important antioxidants, ascorbate and glutathione contents in sunflower leaves exposed to As were significantly decreased by SA treatment. These results reveal that SA is more effective in alleviating As toxicity at higher concentrations than that at lower concentrations.  相似文献   

Bioremediation of heavy metals from municipal sewage by cyanobacteria and its effects on growth and some metabolites of Beta vulgaris     

Mustafa A. Fawzy  Abdel Kareem S. H. Mohamed 《Journal of plant nutrition》2017,40(18):2550-2561

The present research was done to study the ability of cyanobacterial species for removing heavy metals from sewage. As well, to estimate the growth and some metabolites of Beta vulgaris irrigated with sewage treated by cyanobacterial species. The best removal results were obtained by Anabaena oryzae compared to the other studied cyanobacteria. Whereas A. oryzae showed high removal efficiency for cadmium (Cd²⁺) followed by lead (Pb²⁺), zinc (Zn²⁺), iron (Fe²⁺), copper (Cu²⁺) and manganese (Mn²⁺) (88.5, 83.1, 68.8, 62.0, 55.2 and 42.4%, respectively). Irrigation of plants by untreated or treated sewage generally caused stimulation in the total proteins, proline, carbohydrates and ascorbic acid. B. vulgaris grown in soil irrigated with untreated sewage showed maximum catalase, peroxidase activity, hydrogen peroxide (H₂O₂) and lipid peroxidation compared to the other treatments. The heavy metals availability was relatively low in the plant irrigated with treated sewage by cyanobacterial species, so the antioxidants requirement was low and hence the induction of antioxidants was lower compared to the plant irrigated with untreated sewage.  相似文献   

Interactive effect of salinity and silver nanoparticles on photosynthetic and biochemical parameters of wheat     

Abdel Kareem S. H. Mohamed  Muhammad Farooq Qayyum  Ahmed Mustafa Abdel-Hadi  Rabia Abdur Rehman  Shafaqat Ali 《Archives of Agronomy and Soil Science》2017,63(12):1736-1747

The present study investigated the influence of seed priming with silver nanoparticles (Ag NPs), 0, 2, 5 and 10 mM, on growth and biochemical parameters of wheat (Triticum aestivum L.) under salt stress. As expected, 150 mM of NaCl decreased the shoot fresh and dry weights and chlorophyll contents and increased the catalase (CAT) and peroxidase (POD) activities. Salinity enhanced the concentration of proline, soluble sugars, malondialdehyde and hydrogen peroxide. Seed priming with Ag NPs increased the shoot fresh and dry weight of normal and salt-stressed plants. Lower concentration of Ag NPs decreased the total soluble sugars and proline contents, while the higher Ag NPs levels increased these contents compared to the control. The combined application of Ag NPs and salt stress increased the soluble sugars and proline contents, while it decreased CAT activity and increased POD activity compared to the respective Ag NPs treatments alone. Overall, our results demonstrated that Ag NPs enhanced the salt tolerance in wheat, but the long-term response of Ag NPs under salt stress needs further investigation.  相似文献   

Phytosynthesis of Silver Nanoparticles using Leaf Extracts from <Emphasis Type="Italic">Ocimum basilicum</Emphasis> and <Emphasis Type="Italic">Mangifira indica </Emphasis>and their Effect on some Biochemical Attributes of <Emphasis Type="Italic">Triticum aestivum</Emphasis>     

Hanan H. Latif  Mohamed Ghareib  Medhat Abu Tahon 《Gesunde Pflanzen》2017,69(1):39-46

In this investigation, leaf extracts of Ocimum basilicum and Mangifira indica were used as reducing agents for biosynthesis of silver nanoparticles (AgNPs). The biosynthesized AgNPs were authorized by UV-vis spectrophotometry and X?ray diffraction (XRD) analysis. AgNPs were obtained after 5?min of reaction at 80^oC. The formation of AgNPs was confirmed by the presence of absorption peaks at 439?nm using extract from O. basilicum and at 442.5?nm from M. indica. X?ray spectra showed strong peaks for the crystalline Ag. Shape and size of the biosynthesized AgNPs were studied using high resolution transmission electron microscope (HR-TEM). Size of the produced AgNPs was found to be 9–35?nm. Effect of the synthesized nanosilver was then investigated on some biochemical attributes of wheat plant (Triticum aestivum cultivar saka 92). The growth parameters such as shoot lengths, fresh and dry weight of shoot, chlorophyll, carbohydrate and protein contents in shoot of wheat plant were investigated. Application of AgNPs synthesized from Ocimum basilicum and from Mangifira indica at the concentrations of 20,40?ppm showed an increase in shoot length, fresh and dry weight of shoot, chlorophyll, total carbohydrate and protein content in shoot of wheat plants, beyond these concentrations an inhibitory effects were shown.  相似文献   

Characterization of a unique copper resistance gene cluster in <Emphasis Type="Italic">Xanthomonas campestris</Emphasis> pv. <Emphasis Type="Italic">campestris</Emphasis> isolated in Trinidad,West Indies     

Franklin Behlau  Alberto M. Gochez  Alshia Janel Lugo  Winston Elibox  Gerald V. Minsavage  Neha Potnis  Frank F. White  Mohamed Ebrahim  Jeffrey B. Jones  Adesh Ramsubhag 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(3):671-681

Whole genome sequencing of a copper resistant (Cu^R) black rot strain of Xanthomonas campestris pv. campestris (Xcc) isolated from a broccoli plant in Trinidad revealed a unique operon for copper resistance. The cop genes of strain Xcc-BrA1 were determined to be present on a 160 to 180 kb plasmid shown to be non-conjugative with other xanthomonads. While nucleotide comparison of a putative 8.0 Kbp copLABMGF gene cluster identified in Xcc-BrA1 genome did not reveal any homologous region with other known Cu^R Xanthomonas strains from diverse origins, the comparison of the translated amino acid sequence indicated similarity with X. citri, X. c. pv. citrumelonis and X. vesicatoria Cop proteins. Cloning of the copLAB gene cluster from Xcc-BrA1 conferred copper resistance to other copper-sensitive xanthomonads. Although Xcc-BrA1 harbors copLAB genes with similar sizes and organization and is able to grow on Cu-amended medium as other Cu^R xanthomonads, the phylogenetic analysis of nucleotide sequences indicates that the cop cluster in Xcc-BrA1 is unique and distantly related to other copLAB genes from Xanthomonas and Stenotrophomonas. The origin of copper resistance genes in Xcc-BrA1 is likely a result of horizontal gene acquisition from a still unknown phylloplane cohabitant. The findings of this study have implications for the management of crop diseases caused by Cu^R xanthomonads. Future studies could focus on and determining the distribution, overall importance and appropriate control measures for strains harbouring these unique genes.  相似文献   

Molecular and experimental evidence of multi-resistance of <Emphasis Type="Italic">Cercospora beticola</Emphasis> field populations to MBC,DMI and QoI fungicides     

Nenad R. Trkulja  Anja G. Milosavljević  Milana S. Mitrović  Jelena B. Jović  Ivo T. Toševski  Mohamed F. R. Khan  Gary A. Secor 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(4):895-906

Binucleate Rhizoctonia (BNR) spp. isolates were collected from taro (Colocasia esculenta (L.) Schott) and ginger (Zingiber officinale (Willd.) Roscoe) (Yunnanxiaojiang cv.) in Yunnan province. These Yunnan (YN) isolates did not anastomose with any of the tester isolates of the known AGs of binucleate Rhizoctonia spp. The growth of YN cultures on PDA was appressed, mealy and matlike after 4 days of incubation, then turned white brown, producing brown to dark brown, irregularly shaped sclerotia were embedded in the PDA medium after 14 days. All attempts to induce basidiospore production were unsuccessful, but the length and sequence of the internal transcribed spacer (ITS1 + 5.8S rDNA + ITS2) regions of 5.8S rDNA from the YN isolates were identical in length and sequence to isolates of all the other AGs of binucleate Rhizoctonia /Ceratobasidium spp. The sequences of 5.8S rDNA-ITS from the YN isolates were unique among AGs of BNR. The YN isolates had sequence similarities of 94% with isolates of AG Fb and P, 93% with AG E, 91% with AG R, 79–94% with AG S, and 74–87% with AG A, Ba, Bb, Bo, C, DI, DII, DIII, Fa, G, H, I, K, L, O, and Q. Four isolates of AG YN caused minor virulence (lesions ≦1mm²⁾ to ginger or taro in growth chamber studies. It was concluded that the YN isolates belong to a new anastomosis group AG-V of the Ceratobasidium spp..  相似文献