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111.
Reihaneh Alsadat Mahmoudian Maryam Lotfi Gharaie Roya Abbaszadegan Mohammad Mahdi Forghanifard Mohammad Reza Abbaszadegan 《Iranian Biomedical Journal》2021,25(3):157
Background:LINC-ROR, as a cancer-related lncRNA, has vital roles in stem cell survival, pluripotency, differentiation, and self-renewal in hESCs. However, cancer-related molecular mechanisms, its functional roles, and clinical value of LINC-ROR in GC remain unclear. In this study, we aimed to investigate probable interplay between LINC-ROR with SALL4 stemness regulator and their role with the development of the disease. Methods:The mRNA expression profile of LINC-ROR and SALL4 was assessed in tumoral and adjacent non-cancerous tissues of GC patients, using quantitative real-time PCR. Results:Significant LINC-ROR underexpression and SALL4 overexpression were observed in 55.81% and 75.58% (p < 0.0001) of samples, respectively. The expression of LINC-ROR and SALL4 were significantly correlated with each other (p = 0.044). There was an association between the underexpression of LINC-ROR and sex, stage of tumor progression, tumor type, and location of tumor (p < 0.05), and H. pylori infection with SALL4 expression (p = 0.036). There were also significant correlations between concomitant mRNA expression of SALL4 and LINC-ROR in tumors located at distal noncardiac, positive for H. pylori infection, tumors with invasion into the muscle layer of the stomach, and grade II tumor (p < 0.05). Conclusion:The clinical results of the SALL4-LINC-ROR association propose a probable functional interaction between these markers in tumor maintenance and aggressiveness. Our study can help to understand one of the mechanisms involved in the progression of GC through the function of these regulators. Key Words: LINC-ROR, Non-coding RNA, SALL4 相似文献
112.
Maryam Ebrahimiyan Mohammad Mahdi Majidi Aghafakhr Mirlohi Abbas Noroozi 《Euphytica》2013,190(3):401-414
The physiological basis of genetic variation in drought response and its association with yield and related indices is not clear in tall fescue. In this study thirty genotypes of tall fescue (Festuca arundinacea Schreb.) were sampled from a polycross population and evaluated under two levels of irrigation in 2010 (normal and intense stress) and 2011 (normal and mild stress). Physiological traits including relative water content (RWC), total chlorophyll (TChl), chlorophyll a (Chla), chlorophyll b (Chlb), Chla/Chlb, carotenoids (Car), TChl/Car and proline content along with forage yield, agro-morpholgical traits and selection indices (stress tolerance index, STI and drought susceptibility index, DSI) were studied. Large variation and moderate to high heritability was estimated for most of the studied traits. Intense drought condition decreased chlorophyll content while mild stress significantly increased it. In the other hand intense drought stress increased Chla/b while mild stress didn’t change it. Under mild drought stress condition STI was positively correlated with RWC while under intense drought stress condition STI was positively correlated with chlorophyll content. Although proline content was significantly increased in both intense and mild drought stress conditions, no relationship was found between proline accumulation with forage yield and STI. Applications of principle component analysis for screening suitable genotypes are also discussed. 相似文献
113.
Background: Infectious by Pseudomonas aeruginosa has spread worldwide and metallo-beta-lactamases (MBL) are being reported with increasing frequency. The aim of this study was to investigate the antibiotic susceptibility and distribution of blaVIM and blaIMP genes in P. aeruginosa isolates from Zanjan Province of Iran. Methods: A total of 70 P.
aeruginosa isolates were identified from patients admitted at intensive care units. The antimicrobial susceptibility was tested by disk diffusion (Kirby-Bauer) method and for production of MBL using double-disk synergy test (DDST). After DNA extraction, the presence of blaVIM and blaIMP genes and class 1 integron were detected by PCR. RESULTS: Most of the isolates were resistant to meropenem, cefotaxime and imipenem (IPM). Also, 44/70 (62.85%) IPM resistant isolates were confirmed by DDST. Of the 44 clinical isolates, 41 (93%) isolates showed MIC≥4 µg/ml for IPM. Based on the DDST results, 36 (87.8%) were confirmed to be MBL producers. PCR amplification showed that 23/41 (56%) carried blaVIM and 10/41 (24.3%) possessed blaIMP gene. Also, 31/44 (70.5%) isolates contained class 1 integron gene. Conclusion: Our results highlight that the genes for Verona integron-encoded metallo-β-lactamase, IPM β-lactamases and class 1 integrons were predominantly present among the IPM-resistant P. aeruginosa tested in our province and also the frequency of blaVIM type is higher than blaIMP. This is the first report of P. aeruginosa strains producing blaIMP with high frequency from Zanjan province of Iran. Key Words: Pseudomonas aeruginosa, Beta-lactamases, PCR 相似文献
114.
115.
Nayereh Nouri Narges Nouri Omid Aryani Behnam Kamalidehghan Maryam Sedghi Massoud Houshmand 《Iranian Biomedical Journal》2012,16(4):223-225
Background
Ataxia with oculomotor apraxia type 1 (AOA1) shows early onset with autosomal recessive inheritance and is caused by a mutation in the aprataxin (APTX) gene encoding for the APTX protein.Methods
In this study, a 7-year-old girl born of a first-cousin consanguineous marriage was described with early-onset progressive ataxia and AOA, with increased cholesterol concentration and decreased albumin concentration in serum. PCR and direct DNA sequencing was performed after DNA extraction.Results
Sequencing analysis revealed a novel homozygous deletion in c.643 and A>T single nucleotide polymorphism in c.641 in exon 6 of the APTX gene [ENST00000379825].Conclusion
It seems that this region of exon 6 is probably a hot spot; however, no deletions have been reported in exon 6 yet. Key Words: Ataxia oculomotor apraxia 1 (AOA1), aprataxin (APTX), Iranian 相似文献116.
Sana Eybpoosh Yeganeh Talebkhan Samaneh Saberi Maryam Esmaeili Akbar Oghalaie Fatemeh Ebrahimzadeh Toktam Karimi Afshin Abdirad Azin Nahvijou Mohammad Ali Mohagheghi Mahmoud Eshagh Hosseini Marjan Mohammadi 《Iranian Biomedical Journal》2015,19(3):133-142
Background:
Serologic screening of gastric cancer (GC) by serum pepsinogens (sPG) levels and Helicobacter pylori (Hp) sero-status, though highly informative, has provided heterogeneous results. Here, we have evaluated the modifying effects of demographic factors on the risk impact of Hp sero-status/sPG levels in gastric cancer, with particular emphasis on age.Methods:
A cross-sectional study was carried out on 1341 individuals (GC = 578, healthy = 763), who were stratified into two age groups: 35-59 years (middle-aged, n = 830) and ≥ 60 years (60 years-plus, n = 511). Demographic factors and serological states (Hp sero-staus and sPG levels) were recorded by subject interview and serum ELISAs, respectively. Covariate-specific odds ratios were calculated by multivariable logistic regression.Results:
Hp infection was consistently associated with increased sPGI and sPGII levels in the 60 year-plus, but not the middle-aged group. The joint examination of the variable states of the three serum biomarkers (Hp serology, sPGI, and sPGI/II ratio), in the 60 year-plus age group, demonstrated a stepwise escalation of risk from the single (sPGIlow; OR = 2.6), to double (sPGIlow/sPGI/IIlow; OR = 3.55, and Hppositive/sPGIlow; OR = 5.0) and ultimately triple (Hppositive/PGIlow/PGI/IIlow; OR = 10.48) positive states, in reference to the triple negatives. However, this pattern was not exhibited in the middle-aged subjects.Conclusion:
Age was clearly identified as a modifying factor on the risk projection of the combined states of Hp serology and sPG levels in gastric cancer screening, reflected by the augmented (~10.5 fold) risk of GC in the triple positive (Hppositive/sPGIlow/sPGI/IIlow) 60 year-plus subjects, which was not evident in the middle-aged group. Key Words: Biomarkers, Demography, Age Distribution 相似文献117.
118.
Maryam Haghighi Jaime A. Teixeira da Silva 《Communications in Soil Science and Plant Analysis》2016,47(2):142-155
An experiment was established to assess the ability of selenium (Se) to reduce cadmium (Cd) toxicity when tomato was grown hydroponically. A factorial experiment was arranged in a completely randomized design with six replicates in cucumber (Cucumis sativus cv. 4200). The Se was applied at four levels [0 mg L–1 (Se0), 2 mg L–1 Se (Se1), 4 mg L–1 Se (Se2), and 6 mg L–1 Se (Se3)], whereas Cd was applied at three levels [0 µM Cd (Cd0), 5 µM Cd (Cd1), and 7 µM Cd (Cd2)]. The Se improved the dry weight of roots even when plants were exposed to Cd. Treatment Se1 improved the dry weight of shoots in Cd1 and Cd2. Treatments Se1 and Se2 improved photosynthesis in Cd1. Treatment Se1 significantly improved stomatal conductance in Cd2 at all levels of Se relative to Cd2. The greatest Cd concentration in leaves was observed in Cd2 × Se0 and while Se concentration in solution increased in response to Se1, Se2, and Se3. The greatest Se level reduced Cd uptake the most. Growth and photosynthetic attributes can be negatively affected by Cd, but Se has the ability to buffer, or improve, several attributes. 相似文献
119.
The application of biosolids to agricultural fields is becoming increasingly common. The effect of biosolids on the behavior of metals in different plants has been reported to be variable and ambiguous. A greenhouse experiment was conducted on a soil spiked with four rates of copper (Cu) (0, 50, 100, and 150 mg kg?1) and zinc (Zn) (0, 150, 300, and 450 mg kg?1) in lettuce culture. When Cu and Zn were spiked to anaerobically-digested biosolids, their availability in lettuce increased. Root and shoot fresh weight decreased due to a decrease in photosynthetic rate. superoxide dismutase (SOD) and peroxidases (POD) activity increased after the application of Cu and Zn. The bioavailability of Zn in lettuce was greater than that of Cu because of a higher transfer factor in plants and due to a higher endogenous Zn concentration.These results will allow for better fertilization management when biosolids are applied to tomato culture. 相似文献
120.
Maryam Zahedifar Mahshid Shafigh Zahra Zarei Fereshteh Karimian 《Archives of Agronomy and Soil Science》2016,62(5):663-673
Potassium (K) is one of the major essential nutrient elements whose application of organic or nano-chelate-fertilizers has received increased attention recently. Cadmium (Cd) contamination in agricultural soils and environment is increasing due to the over-application of Cd-containing phosphate fertilizers. But few studies have been carried out on the environmental influences of K-nano-chelate fertilizers especially on Cd-polluted soils. Therefore, the effects of K-fertilizer application in different rates (0, 100 and 200 mg kg?1 soil) and forms (KCl, K2SO4 and K-nano-chelate) on Cd content and partitioning in Ocimum basilicum grown on an artificially Cd-contaminated calcareous soil (with 40 mg Cd kg?1 soil) were studied under greenhouse conditions. Cadmium decreased shoot dry weight (SDW), but did not affect root dry weight (RDW) and no consistent trend was observed with applied K. Cadmium increased shoot and root Cd concentration or uptake. KCl and K2SO4 increased shoot Cd concentration compared to that of control, whereas K-nano-chelate did not affect it. In Cd-treated soils the mean value of Cd translocation factor (ratio of Cd concentration in shoots to that of roots) decreased by 60% as compared to that of the control. Application of 100 mg K-K2SO4 and 100 and 200 mg K-nano-chelate increased the Cd translocation factor by 49, 59 and 112% in Cd-treated soils, respectively. In Cd-treated soils, greater amounts of Cd accumulated in roots. K-nano-chelate could mitigate the adverse effect of Cd on SDW and Cd accumulation in plants grown on Cd-polluted soils, so the risk of Cd entrance to the food chain is reduced (however, in Cd-untreated soils, K-nano-chelate increased the Cd translocation factor higher than other K sources). In Cd-polluted soils KCl was the most inappropriate fertilizer that may intensify Cd accumulation in plants. However, it may be useful in the phytoremediation of Cd-polluted soils. 相似文献