Seroepidemiologic survey of Coxiella burnetii and attempt to detect Coxiella DNA in aged non-laying chickens in a prefecture of Japan where poultry farming prospers     

Muramatsu Y  Hukuta K  Satoh S  Muramatsu M  Nishimura M  Nagahata H  Ueno H  Morita C  Tamura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(9):1007-1008

The indirect fluorescent antibody test (IFAT) revealed seropositivity to Coxiella burnetii in aged non-laying chickens in poultry farms in a prefecture in the central part of Japan. Seropositivity was 7%, and antibody titers ranged from 16 to 64. No DNA fragment specific for C. burnetii was detected in the chickens by nested-PCR. The prevalence of C. burnetii infection in a prefecture of Japan in which poultry farming prospers was 7%.  相似文献   

Evaluation of myelotoxicity in dietary restricted rats     

Miyata H  Asanuma F  Iwaki Y  Kimura M  Matsumoto K 《Journal of toxicologic pathology》2009,22(1):53-63

The purpose of this study was to clarify the effect of decreased food consumption on evaluation of myelotoxicity in routine general toxicity studies. Male rats were divided into the following 7 groups: 12, 15, and 18 mg/kg 5-fluorouracil (5-FU) treatment groups (FU12, FU15 and FU18); dietary restriction groups (R12, R15 and R18 receiving the same amount of food as the rats in the FU12, FU15 and FU18 groups, respectively); and a nontreated control group (NT). We compared the changes in body weight, hematology and the results of cytological analyses of bone marrow and histopathology among the groups after administration and recovery periods of 14 and 7 days, respectively. At the end of the administration period, the FU15 and FU18 groups showed decreases in many hematologic and bone marrow parameters that were all similar to those in the corresponding dietary restriction groups (R15 and R18). A granulocyte abnormality (polyploidy: frequency of 1% or less) was also observed in all 5-FU treated groups. At the end of the recovery period, increases in the reticulocyte and platelet counts and extramedullary hematopoiesis of the spleen were observed in the 5-FU treated groups. These results indicate that the results of general toxicity studies in rats should be evaluated in consideration of dietary restriction effects when food consumption is decreased at about 30-40% or more. Careful morphological observation of hemocytes would be helpful in distinguishing the effect of a drug from that of dietary restriction in relation to hematological and bone marrow parameters. Performance of a recovery test to determine the reactive response of hematopoiesis is also recommended.  相似文献   

Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese Black cattle herds     

Hideki OHSAKI  Atsuko TANAKA  Shogo HOASHI  Shinji SASAZAKI  Kenji OYAMA  Masaaki TANIGUCHI  Fumio MUKAI  Hideyuki MANNEN 《Animal Science Journal》2009,80(3):225-232

Fatty acid composition of beef adipose tissue is one of important traits because high proportions of monounsaturated fatty acid are related to favorable beef flavor and tenderness. In this study, we investigated effects of genetic factors such as stearoyl-CoA desaturase (SCD) and sterol regulatory element binding protein (SREBP) on beef carcass traits including fatty acid composition using two cattle populations. Sire effect was significantly related to almost all traits except BMS, suggesting that the trait examined in this study is highly controlled by genetic factors. The effect of SCD genotype on fatty acid composition was detected remarkably in both cattle groups, especially on stearic and oleic acids. This result was consistent with our previous studies and suggests that SCD is associated with fatty acid composition. Unlike SCD genotyping, the effect of SREBP genotype was not identified in this study. Our results suggested that SCD genotype would contribute to improving beef quality in field populations. Further studies about the relationship among these factors will bring an insight into the molecular mechanism of fatty acid metabolism in cattle.  相似文献   

Case report: The case of a 17 kg ovarian granulosa cell tumor in a Breton draft mare     

Munkhtuul TSOGTGEREL  Masaaki TAGAMI  Kenichi WATANABE  Harutaka MURASE  Yuko HIROSAWA  Yoshiyasu KOBAYASHI  Yasuo NAMBO 《Journal of Equine Science》2021,32(2):67

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Transient uplift after a 17th-century earthquake along the Kuril subduction zone     

Sawai Y  Satake K  Kamataki T  Nasu H  Shishikura M  Atwater BF  Horton BP  Kelsey HM  Nagumo T  Yamaguchi M 《Science (New York, N.Y.)》2004,306(5703):1918-1920

In eastern Hokkaido, 60 to 80 kilometers above a subducting oceanic plate, tidal mudflats changed into freshwater forests during the first decades after a 17th-century tsunami. The mudflats gradually rose by a meter, as judged from fossil diatom assemblages. Both the tsunami and the ensuing uplift exceeded any in the region's 200 years of written history, and both resulted from a shallow plate-boundary earthquake of unusually large size along the Kuril subduction zone. This earthquake probably induced more creep farther down the plate boundary than did any of the region's historical events.  相似文献   

Polymerase chain reaction-based genetic typing of Japanese porcine reproductive and respiratory syndrome viruses.     

Masaaki Yoshii  Yoshihiro Kaku  Yosuke Murakami  Mitsugu Shimizu  Kanako Kato  Hidetoshi Ikeda 《Journal of veterinary diagnostic investigation》2004,16(4):342-347

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Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture,Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism     

Yuma SAHASHI  Masaaki OSHIMA  Junjiro YAMAGISHI  Chieko MURAMATSU  Kaori SHIMIZU  Yasuo INOSHIMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1730

Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences were, therefore, not new genotypes.  相似文献   

An application of Neurogenetic Algorithm System to individual tree growth model     

Weiming Liao  Keiitirou Nogami  Masaaki Imanaga 《Journal of Forest Research》1998,3(2):79-84

A neural network system with genetic algorithms (Neurogenetic Algorithm System, or NGAS) was employed to develop individual coniferous tree growth models. A multivariable regression model was applied to compare the performance of NGAS. An IBM personal computer with the BioComp System’s software program of NGO was used to execute this comparison. The results indicate that NGAS is more accurate and effective than the conventional regression method in modeling individual tree growth based on the criteria of Sum of Squared Error (SSE), Average Absolute Error (AAE), Mean Squared Error (MSE) and Final Predicted Error (FPE). This study also suggests that individual tree growth may indeed be a non-linear process. Using this flexible neural network system to model individual tree growth can yield satisfactory prediction results.  相似文献   

氮杂磷烯化合物的合成及³¹PNMR表征     

安德烈  吉藤正明 《湖南农业大学学报(自然科学版)》1999,26(5)

通过相应膦二氯化物与２,４,６－三叔丁基苯基氨基锂的反应,再经１,８－二氮双环「５．４．０」－７－十一烯脱氯化氢合成了两个新的氮杂磷烯化合物。与二磷烯比较,分析了它们在核磁共振磷谱中化学位移的变化。  相似文献   

Small-scale variation in feeding environments for the Manila clam <Emphasis Type="Italic">Ruditapes philippinarum</Emphasis> in a tidal flat in Tokyo Bay     

Satoshi Watanabe  Satoshi Katayama  Masashi Kodama  Naritoshi Cho  Kaoru Nakata  Masaaki Fukuda 《Fisheries Science》2009,75(4):937-945

The relative contribution of particulate organic matters (POMs) in water column and sediment as a food source for the Manila clam, Ruditapes philippinarum, was studied using carbon and nitrogen stable isotopic method (δ¹³C and δ¹⁵N) in a tidal flat at Seaside Park, Yokohama, Japan. Comparisons of δ¹³C and δ¹⁵N among R. philippinarum and POMs in surface water, bottom water, and sediment surface indicated that R. philippinarum larger than 5 mm shell length (SL) mainly assimilated benthic POM, and individuals smaller than 5 mm SL assimilated benthic and pelagic POM. Continuous measurements of chlorophyll concentrations in the bottom water revealed tide-driven resuspension of the benthic phytopigments. R. philippinarum showed differences in δ¹³C and δ¹⁵N along an inshore–offshore transect, indicating small-scale spatial differences in POM provision in the tidal flat. These findings suggest that POM in the bottom water, supposedly inhaled by R. philippinarum, is a mixture of a larger proportion of resuspended benthic POM and a smaller proportion of pelagic POM, and that the mixing ratio of the POMs may be affected by the hydrodynamics of flooding water associated with tidal flat topography.  相似文献