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排序方式: 共有363条查询结果,搜索用时 15 毫秒
31.
Sato T Takada M Shibuya H Shirai W Matsuo K 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2004,51(1):23-26
'Granular cell' tumor observed in the malar subcutis of a Djungarian hamster was examined to determine its cellular origin. Histologically, the tumor consisted of a solid growth of oval or spindle-shaped large cells with abundant cytoplasm filled with eosinophilic granules that were periodic acid-Schiff-positive and diastase-resistant. Immunohistochemically, the tumor cells were positive to anti-vimentin and anti-desmin antibodies and a few cells showed positivity to anti-actin antibody as well. They did not react to myoglobin, S-100 protein, and glial fibrillary acidic protein (GFAP). Electron microscopic studies revealed that the tumor cells had pinocytotic vesicles, dense plaque and microfilaments. The first granular cell variant of myogenic tumor reported here in Djungarian hamsters was differentiated from granular cell tumor of Schwann cell origin. 相似文献
32.
Kato Y Asano K Mizutani I Konno T Sasaki Y Kutara K Teshima K Edamura K Kano R Suzuki K Shibuya H Sato T Hasegawa A Tanaka S 《Research in veterinary science》2006,81(2):280-286
The angiopoietin (Ang) family of proteins are central to the regulation of angiogenesis. The purposes of this study were to determine cDNA sequences of canine Ang-1 and Ang-2 and investigate their expressions in normal tissues and spontaneous tumours. The cDNA sequences of canine Ang-1 and Ang-2 were 1,494 and 1,488 bp, and the deduced amino acid sequences were 497 and 495 residues, respectively. The cDNA sequences of canine Ang-1 and Ang-2 showed high homology with those of the other mammalian species. Canine Ang-1 and Ang-2 mRNA were detectable in all 22 normal tissues and spontaneous tumours. Higher mRNA expression level of canine Ang-2 was demonstrated in mammary simple carcinomas, haemangiosarcoma and hepatocellular carcinoma in comparison with normal tissues. 相似文献
33.
Takeda M Kasai H Torii Y Mukamoto M Kohda T Tsukamoto K Kozaki S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(4):325-330
Avian botulism is a paralytic disease caused by a toxin produced by Clostridium botulinum type C. Since type C isolates from cases of avian botulism produced a neurotoxin consisting of a mosaic form of parts of type C and D neurotoxins, we examined the antitoxin titers in the convalescent sera of botulism-affected birds which belonged to family Anatidae. ELISA using the C/D mosaic neurotoxin as an antigen revealed that the antibody was detected in the sera at 2 weeks, but not at 5 weeks after the onset, suggesting that the antibody only appeared for a short period in the convalescent phase. However, we failed to detect the antibody titers with anti-chicken IgG instead of anti-duck IgG. We therefore examine the immunological properties of IgG among different families and species. The results revealed that different species of IgG in the same family exhibited strong cross-reactivity. Ducks immunized once with the toxoid together with a commercial oil-adjuvanted vaccine were found to develop sufficient antibody to protect against a challenge with a lethal toxin dose. The ELISA titers did not correspond to the neutralization titers in the sera of immunized ducks at the early stage during immunization. These findings suggest that the neutralizing titer was more useful than the ELISA titer for evaluating the protection against the toxin, but the ELISA technique may be applicable for detecting the occurrence of botulism. 相似文献
34.
Sakai M Harada K Matsumura H Takahashi T Koie H Yamaya Y Watari T Shibuya H Sato T Tokuriki M Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(12):1331-1333
A 14-year-old, spayed female, domestic shorthair cat was referred to us with anorexia, pyrexia, and jaundice. Total bilirubin (TBIL) and feline trypsin-like immunoreactivity (fTLI) levels were remarkably high. Based on laparoscopic biopsy of the pancreas, the cat was diagnosed as having pancreatitis. As a result of treatment with a synthetic protease inhibitor and corticosteroid, the TBIL and fTLI values returned to normal and the clinical course was good. 相似文献
35.
Sakai M Otani I Ishigaki K Edamura K Takahashi T Koie H Yamaya Y Watari T Shibuya H Sato T Kanayama K Tokuriki M Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1219-1221
We evaluated hepatic T lymphocyte phenotypes in a dog with chronic hepatitis. Before treatment, numerous CD3+ lymphocytes were demonstrated in the liver, and the ratio of CD4+/CD8+ was remarkably high (2.96; reference range, 0.33+/-0.12). After treatment, CD3+ lymphocyte infiltration in the liver was reduced, and the ratio of CD4+/CD8+ decreased to 0.31. Therefore, hepatic T lymphocytes, especially CD4+ lymphocytes, might play a central role in the pathogenesis of this dog with chronic hepatitis. 相似文献
36.
Asano K Sakata A Shibuya H Kitagawa M Teshima K Kato Y Sasaki Y Kutara K Seki M Edamura K Sato T Tanaka S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(9):1003-1006
Fibrodysplasia ossificans progressiva (FOP)-like condition was diagnosed in a Japanese domestic cat with stiffness, marked atrophy of the muscles, and limited mobility of all joints in both the pelvic limbs. Etretinate, a retinoid, was used for medical management; however, no improvement in the clinical signs was observed. Inheritance of the disorder has not yet been demonstrated. Furthermore, the clinical signs and histopathological findings of feline FOP-like condition in the present case differed from those of the previously reported cases. 相似文献
37.
Haraguchi T Tani K Koga M Oda Y Itamoto K Yamamoto N Terai S Sakaida I Nakazawa H Taura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):633-636
Autologous bone marrow stromal cells (BMSCs) infusion therapy improves the hepatic fibrosis. To investigate the mechanism of remission, we evaluated the matrix metalloproteinase (MMP)-2 and -9 activity in canine BMSCs and the effect of pro-inflammatory cytokines on their expression. The activity and the gene expression of MMPs were analyzed by gelatin zymography and quantitative RT-PCR, respectively. The specific gelatinase bands were indicative effect of MMP-2 and -9 in canine BMSCs. MMP-2 expression seemed to be increased by TNF-α and IL-1β while MMP-9 was enhanced by TNF-α and IL-6. These results suggested that remissive effect on liver fibrosis might be partly attributable to the MMP-2 and -9 activity in BMSCs under the inflammatory condition. 相似文献
38.
Hashino M Kim S Tachibana M Shimizu T Watarai M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(8):1075-1077
The mechanisms of abortion and sterility induced by bacterial infection are largely unknown. In the present study, we found that Brucella abortus, a causative agent of brucellosis and facultative intracellular pathogen, caused sterility in pregnant mice. We have recently established a mouse model for abortion induced by B. abortus infection and high rates of abortion are observed for bacterial infection on day 4.5 of gestation, but not for other days. Infected newborn (first generation) mice showed poor growth compared with uninfected newborn mice and bacterial replication in the spleen of the former was observed over a long period. When infected first generation female mice were mated to infected first generation male mice, the number of fetuses was significantly less than that in uninfected first generation mice. These infected second generation mice also showed poor growth. These results suggest that vertical transmission of B. abostus causes sterility in pregnant mice and our mouse model would be useful for the investigating of brucellosis. 相似文献
39.
Both stem and crown mass affect tree resistance to uprooting 总被引:1,自引:0,他引:1
Tsutomu Urata Masato Shibuya Akio Koizumi Hiroyuki Torita JooYoung Cha 《Journal of Forest Research》2012,17(1):65-71
To examine the hypothesis that both stem and crown mass affect the resistance of a tree to uprooting and that tree resistance
increases with increasing crown mass, we conducted tree-pulling experiments on three Picea glehnii plantations (stands A, B, and C: 27–32 years old) that differed in tree density and slenderness ratio. Allometries between
crown and stem masses and between the critical uprooting moment and stem mass differed significantly among the three stands,
with the crown mass and critical moment significantly larger in stand C than in stands A or B, despite the same stem mass.
These results quantitatively verified our hypothesis. Allometries between crown and stem masses and between critical uprooting
moment and stem mass were highly significant in each stand but were stand specific. Therefore, these allometries can be used
to estimate tree resistance to uprooting in a given stand but not for data compiled from stands of various conditions and
tree shapes. The allometry between critical moment and aboveground mass did not differ among the three Picea stands; thus, it is not stand specific and is generally appropriate to use for estimating tree resistance. To increase tree
resistance to uprooting, we recommend light management for Picea glehnii plantations and probably other coniferous plantations as well. 相似文献
40.
Mariko Takano Muneysohi Yamaguchi Hiroaki Sano Masaya Nakamura Hajime Shibuya Yasumasa Miyazaki 《Journal of Wood Science》2013,59(2):141-148
The gene encoding manganese peroxidase of a white-rot fungus Phanerochaete crassa WD1694 was cloned and sequenced. Four genomic clones were sequenced in which 3 clones were existed as alleles. The analysis of intron–exon structures divided the 4 clones into three subfamilies that corresponded to mnp2 and mnp3 of Phanerochaete chrysosporium, and a new subfamily possessing only five introns. The purified P. crassa WD1694 MnP consisted of 4 isozymes with same molecular weight, same N-terminal sequence, and different pI. N-terminal sequence of deduced protein of P. crassa mnpB3 gene was identical to those of 4 MnP isozymes; however, the other 3 mnp genes had different N-terminal sequence. The molecular weight of encoded mature protein of mnpB3 gene and purified MnP had a gap that could be difference between MnP proteins encoded by single gene. The results suggested that 4 MnP isozymes of P. crassa WD1694 arose from single gene. 相似文献