Traumatic intracerebral hematoma in a dog: MR images and clinical findings     

Kitagawa M  Okada M  Kanayama K  Sakai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(8):843-846

A nine-year-old, male, mongrel dog was unsteady after falling down a set of stairs. The dog exhibited a mildly abnormal gait 2 days after injury, and was not able to stand, with spasticity of the right limbs, 4 days after injury. MR imaging revealed a clearly demarcated mass on the top of the left lateral ventricle that showed mild hyperintensity on T1-weighted images and hyperintensity on T2-weighted images. The authors diagnosed the dog as having a traumatic intracerebral hematoma. This type of case, in which the clinical signs deteriorated due to edema associated with hematoma, is extremely rare.  相似文献   

Cloning and expression of canine interferon-alpha genes in Escherichia coli   总被引：1，自引：0，他引：1  

Taira O  Watanugi I  Hagiwara Y  Takahashi M  Arai S  Sato H  Maehara N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(10):1059-1062

We cloned five new subtypes of cDNA encoding canine interferon-alpha (CaIFN-alpha) from a canine epithelial cell line. CaIFN-alphas were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were purified. Recombinant CaIFN-alphas were highly species-specific and showed antiviral activity against Vesicular stomatitis New Jersey virus and canine adenovirus-1 , but not against canine herpesvirus-1.  相似文献   

Cloning of the pathogenicity-related gene <Emphasis Type="Italic">FPD1</Emphasis> in <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">lycopersici</Emphasis>     

Masato?Kawabe  Kohei?Mizutani  Takanobu?Yoshida  Tohru?Teraoka  Katsuyoshi?Yoneyama  Isamu?Yamaguchi  Tsutomu?ArieEmail author 《Journal of General Plant Pathology》2004,70(1):16-20

We selected a reduced-pathogenicity mutant of Fusarium oxysporum f. sp. lycopersici, a tomato wilt pathogen, from the transformants generated by restriction enzyme-mediated integration (REMI) transformation. The gene tagged with the plasmid in the mutant was predicted to encode a protein of 321 amino acids and was designated FPD1. Homology search showed its partial similarity to a chloride conductance regulatory protein of Xenopus, suggesting that FPD1 is a transmembrane protein. Although the function of FPD1 has not been identified, it does participate in the pathogenicity of F. oxysporum f. sp. lycopersici because FPD1-deficient mutants reproduced the reduced pathogenicity on tomato.The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number AB110097  相似文献   

Regulated upon activation normal T-cell expressed and secreted (RANTES) contributes to abortion caused by Brucella abortus infection in pregnant mice     

Watanabe K  Iwai N  Tachibana M  Furuoka H  Suzuki H  Watarai M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(7):681-686

Brucella abortus (B. abortus) is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant cells, and the causative agent of brucellosis. In the present study, we found that production of regulated upon activation normal T-cell expressed and secreted (RANTES) due to B. abortus infection contributes to abortion in pregnant mice. B. abortus infected pregnant interferon-gamma (IFN-gamma) knockout mice died within 15 days of infection, but non-pregnant IFN-gamma knockout mice were still alive. With infection by wild type B. abortus, a large amount of RANTES production was observed in pregnant IFN-gamma knockout mice, and induction of RANTES was also observed in normal pregnant mice infected with the wild type, but not in those infected with the intracellular replication-defective mutant. Production of RANTES and IFN-gamma were inhibited in mice inoculated with the respective RANTES or IFN-gamma antibody. Neutralization of RANTES, induced by B. abortus infection, served to prevent abortion. These results indicate that the production and function of RANTES are correlated with IFN-gamma in pregnant mice infected with B. abortus.  相似文献   

Functional disorder of the retina in manganese-deficient Japanese quail revealed by electroretinography using a contact lens electrode with built-in light source     

Endo K  Itoh N  Maehara S  Shinozaki A  Imagawa T  Uehara M  Mizuno N  Sasaki S  Hiraga T  Teraoka H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(2):139-144

Manganese deficiency results in neurological and skeletal defects, together with ultrastructural disarrangement of the retina in rats. Wild birds show a range of Mn concentrations in their tissues, including the liver, raising the possibility of Mn-related disorders in the wild. Electroretinography (ERG) provides a useful noninvasive approach to evaluate visual function. This method is especially useful in birds, as objective analysis of them is very difficult, while they have well-developed vision. In this study, we carried out a convenient and reliable ERG recording using a contact lens electrode with a built-in light source (LED electrode) of Japanese quail (Coturnix coturnix japonica) fed a Mn-deficient diet. After 10 min light adaptation, single-flash and flicker cone responses were reproducibly recorded to cause an intensity-dependent increase in amplitude of both a-wave and b-wave in single-flash ERG. Mn-deficient feeding markedly decreased the Mn concentration in the liver by almost half in 3 to 6 weeks, followed by body weight loss in 13 to 15 weeks. Implicit time of a-wave and b-wave cone response by single-flash stimulation was significantly delayed in quail with a Mn depletion from 3 to 6 weeks. Every cone response of the Mn-deprived quail had a tendency to decrease amplitude. The ultrastructure of cone photoreceptor cells was disorganized by Mn deficiency, including changes in outer segment discs of photoreceptor cells. These results suggest the essential role of Mn in the integrity of the retinal function of birds.  相似文献   

Application of restriction fragment length polymorphism analysis to simple and rapid genotyping of bovine viral diarrhea virus strains isolated in Japan   总被引：1，自引：0，他引：1  

Seki Y  Seimiya YM  Motokawa M  Yaegashi G  Nagai M  Hayashi M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(4):393-395

The E2 regions of 177 bovine viral diarrhea virus (BVDV) strains isolated in Japan between 1957 and 2006 were analyzed for genotyping. The strains were classified into 8 genotypes (1a, 1b, 1c, 1d, 1e, 1f, So and 2a) based on the phylogenetic analysis. The restriction fragment length polymorphism (RFLP) analysis of the RT-PCR products using 6 selected enzymes (Apo I, Mly I, BstAP I, Pvu II, Ear I, EcoR V) disclosed the cutting patterns classified into 11 groups (I-XI), each of that consisted of strains belonging to a single genotype. Namely, groups-I and -II were composed by genotype-1a strains, groups-III and -IV by 1b strains, and groups-V and -VI by 1c strains. Other groups-VII, -VIII, -IX, -X and -XI comprised genotypes-1d, -1e, -1f, -So and -2a strains, respectively. The results suggest that the RFLP analysis can simply and rapidly differentiate the 8 genotypes of BVDV strains.  相似文献   

Investigation of change in tangential strain on the inner bark of the stem and root ofBetula platyphylla var.japonica andAcer mono during sap season     

Masato Yoshida  Osamu Yamamoto  Takashi Okuyama  Yutaka Tamai  Yuzou Sano  Minoru Terazawa 《Journal of Wood Science》1999,45(5):361-367

Although it is well known that sap exudation during early spring in temperate deciduous trees occurs in response to daytime warming and nighttime cooling, the mechanisms of the process are not yet fully understood. Previous theories suggested that changes in stress in the wood caused by daytime heating and nighttime cooling might be linked with sap flow. Consequently, a study of itaya-kaede maple (Aver mono) and shirakamba birch (Betula platyphylla var.japonica) looked at tangential strains. One-hour intervals for 3 years of the tangential strains on the inner bark of stem and root were measured in itaya-kaede maple and shirakamba birch during the sap exudation season. The measurements indicated different mechanisms of sap exudation in these two trees. During the sap exudation season in late March, when the temperature fluctuated around 0°C, the tangential strain in the root of itaya-kaede maple showed expansion in the daytime and contraction at night. Conversely, in early April the tangential strain in the root of shirakamba birch exhibited contraction in the daytime and expansion at night. The changes in tangential strains in itaya-kaede maple were attributed to conditioning, a known concept used to explain the uptake mechanism of soil water in maple and its exudation during early spring. However, because the change in tangential strain in the roots of shirakamba birch was similar to that found during the rampant season, sap exudation was not attributed to conditioning but to the plentiful supply of water from the roots. The implications of these mechanisms are that different sap harvesting techniques may be appropriate for different tree species.  相似文献   

Negative gravitropism and growth stress in GA3-treated branches ofPrunus spachiana Kitamura f.spachiana cv.Plenarosea     

Masato Yoshida  Hiroyuki Yamamoto  Takashi Okuyama  Teruko Nakamura 《Journal of Wood Science》1999,45(5):368-372

One of the roles of growth stress in branch shape formation was investigated using a weeping-type Japanese cherry,Prunus spachiana. Negative released strains, caused by longitudinal tensile growth stresses, were detected in the upper side of gibberellin A₃-treated (GA₃-treated) and control branches. The mean value of the released strain in the upper side of the treated branches was –0.104%, which was larger than the value (–0.067%) observed in the control branches. Both branches formed tension wood in the upper side of the xylem, and the treated branches formed tension wood near the pith as well. This suggested that the treated branches generated larger tensile growth stress from the early growth stages. The successive generation of growth stress from the early growth stages was considered to generate forces large enough to bend the branch upward.  相似文献   

Occurrence of tetrodotoxin in three Nassarius gastropod species in Khanh Hoa Province,Vietnam     

Dao  Ha Viet  Pham  Ky Xuan  Hoang  Ben Xuan  Tanioka  Masato  Watanabe  Ryuichi  Suzuki  Toshiyuki 《Fisheries Science》2020,86(1):181-186

Fisheries Science - Neurotoxic poisonings with fatal symptoms caused by consumption of Nassarius gastropods have been reported in Vietnam but the causative toxins have not been confirmed. In the...  相似文献   

Oreochromis niloticus aquaculture with biofloc technology,photoautotrophic conditions and Chlorella microalgae     

Yenitze E. Fimbres‐Acedo  Paola Magalln‐Servín  Rodolfo Garza‐Torres  Maurício G. C. Emerenciano  Rosalía Servín‐Villegas  Masato Endo  Kevin M. Fitzsimmons  Francisco J. Magalln‐Barajas 《Aquaculture Research》2020,51(8):3323-3346

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