Stomach wall evaluation using helical hydro-computed tomography     

Terragni R  Vignoli M  Rossi F  Laganga P  Leone VF  Graham JP  Russo M  Saunders JH 《Veterinary radiology & ultrasound》2012,53(4):402-405

In helical hydro-computed tomography (helical hydro-CT), water is used as a neutral luminal contrast medium together with intravenous iodine contrast medium for the diagnosis and staging of human gastric neoplasia. We evaluated the feasibility of helical hydro-CT in 11 healthy animals (nine dogs and two cats). Adequate uniform gastric distension was obtained with 30 ml water/kg body weight. Fourteen client-owned dogs and four cats with suspected or diagnosed gastric neoplasia then underwent helical hydro-CT followed by intravenous contrast medium administration. Focal thickening with moderate contrast enhancement was found in 10 dogs and 3 cats. The extent of the lesion was assessed easily in all these patients. Three dogs and one cat had a normal stomach wall. One dog had multifocal thickening of the antrum but no histopathologic diagnosis was made. Helical hydro-CT, followed by intravenous contrast medium administration, is a simple technique for assessing the stomach wall.  相似文献   

Laparoscopic sterilization of Sardinia donkeys using an endoscopic stapler   总被引：1，自引：0，他引：1  

Pepe M  Gialletti R  Moriconi F  Puccetti M  Nannarone S  Singer ER 《Veterinary surgery : VS》2005,34(3):260-264

OBJECTIVE: To describe a laparoscopic technique for sterilization of the male donkey using an endoscopic linear stapler (ELS). STUDY DESIGN: Clinical report. ANIMALS: Male Sardinia donkeys (n=6), aged 24-36 months. METHODS: Laparoscopic sterilization was performed under standing sedation or recumbent under general anesthesia. An endoscopic linear stapler was used to transect, and control hemorrhage of, the spermatic cord. Serum testosterone concentrations were measured preoperatively and at 3, 6, and 12 months after sterilization. One testis from each of 2 donkeys was collected at 12 months for histologic examination. RESULTS: Sterilization was successfully achieved. The only minor intraoperative complication experienced was hemorrhage from 1 spermatic cord stump, which was re-stapled. No other major short- or long-term complications occurred. Serum testosterone concentrations were < 15 ng/dL at 3, 6, and 12 months after surgery in all donkeys. Testicular atrophy without evidence of revascularization was observed in the 2 testes examined histologically. CONCLUSIONS: Sterilization of descended testes by intraabdominal laparoscopic transection of the spermatic cord is a simple practical method, with minimal intraoperative or postoperative complications. Testicular revascularization did not occur. CLINICAL RELEVANCE: Laparoscopic sterilization in donkey can be performed using an endoscopic linear stapler as an alternative to scrotal castration.  相似文献   

Computed tomography-guided fine-needle aspiration and tissue-core biopsy of bone lesions in small animals     

Massimo Vignoli  DR. MED. VET.  SRV    Stefanie Ohlerth  DR. MED. VET.    Federica Rossi  DR. MED. VET.  SRV    Luigi Pozzi  PROF. DR. MED. VET.    Rossella Terragni  DR. MED. VET.    Daniele Corlazzoli  DR. MED. VET.    Barbara Kaser-Hotz  PROF. DR. MED. Vet 《Veterinary radiology & ultrasound》2004,45(2):125-130

In humans, free-hand computed tomography (CT)-guided biopsy is an accurate method to obtain a tissue sample. There are only a few reports of this technique in veterinary medicine. In the present study, 21 dogs and two cats underwent a free-hand CT-guided tissue-core biopsy (17 animals) or fine-needle aspiration (six animals) of a bone lesion. Two out of 17 tissue-core samples were also cultured. All 17 tissue-core biopsy samples were diagnostic (accuracy of 100%). Five out of six aspirates were diagnostic (accuracy of 83.3%). The overall accuracy was 95.7%. In one aspirate, cytologic quality was insufficient containing only blood. No major complications were encountered. Fourteen neoplastic, two infectious and six benign lesions were diagnosed. CT examination after intravenous contrast medium added useful information to avoid large vessels and to biopsy-viable tissue. Free-hand CT-guided tissue-core biopsy and aspiration appears to be a safe and very accurate procedure for use in the diagnosis of bone-associated diseases in small animals.  相似文献   

Fatal necrotizing fasciitis and myositis in a captive common bottlenose dolphin (Tursiops truncatus) associated with Streptococcus agalactiae.     

Valentina Zappulli  Sandro Mazzariol  Laura Cavicchioli  Claudio Petterino  Luca Bargelloni  Massimo Castagnaro 《Journal of veterinary diagnostic investigation》2005,17(6):617-622

A common bottlenose dolphin (Tursiops truncatus) was presented for necropsy after acute onset of gastrointestinal signs and cutaneous lesions that rapidly progressed to death. Gross and microscopic findings were characterized by locally extensive severe necrohemorrhagic fasciitis and cellulitis, and severe necrotizing myositis in the head and dorsocranial thorax, with numerous disseminated gram-positive cocci. Streptococcus agalactiae was isolated from the lesions and from visceral organs (liver and lung), and it was identified by standard microbiology techniques. This communication is the first report of necrotizing fasciitis in a marine mammal associated with S. agalactiae.  相似文献   

SPINAL SUBARACHNOID CYST IN A CAT     

Massimo  Vignoli  DVM  SRV  Federica  Rossi  DVM  SRV  Giuseppe  Sarli  DVM 《Veterinary radiology & ultrasound》1999,40(2):116-119

A 7-year-old neutered female domestic short hair cat was presented with hind limb ataxia. A subarachnoid cyst in the T10-T11 spinal cord region was identified by myelography as a collection of contrast medium in the subarachnoid space.  相似文献   

Effects of compost and defatted oilseed meals as sustainable organic fertilisers on cardoon (Cynara cardunculus L.) production in the Mediterranean basin     

Domenico Ronga  Domenica Villecco  Massimo Zaccardelli 《The Journal of Horticultural Science and Biotechnology》2019,94(5):664-675

Cardoon (Cynara cardunculus L.) is considered as one of the most suitable energy crops for Southern Europe. The aim of this work was to outline the effects of organic fertilisers on the productivity and the global warming potential (GWP) on cardoon production. Six fertilisers (N 100 kg ha^?1, N 50 kg ha^?1, Compost 30 t ha^?1, Compost 15 t ha^?1 + N 25 kg ha^?1, 3 t ha^?1 of defatted oilseed meals of sunflower, 3 t ha^?1 of defatted oilseed meals of Brassica carinata), and unfertilised control, were evaluated on two cultivars (‘Gobbo di Nizza’ and ‘Altilis 41’) in a split-plot experiment. Defatted oilseed meal of sunflower recorded higher total dry weight (+10%), seed yield (+17%), nitrogen use efficiency (+14%) and better GWP (?66%) compared to the other organic fertilisers and performing as well as N 100 kg ha^?1. Altilis 41 cultivar showed the highest aboveground total dry weight (10 t ha^?1 y^?1), seed yield (1.7 t ha^?1 y^?1), stalk dry weight (7 t ha^?1 y^?1) and head dry weight (3 t ha^?1 y^?1). Our results highlighted that by combining suitable cultivar and fertilisation strategies, it could be possible to increase the production sustainability of C. cardunculus.  相似文献   

PML regulates apoptosis at endoplasmic reticulum by modulating calcium release     

Giorgi C  Ito K  Lin HK  Santangelo C  Wieckowski MR  Lebiedzinska M  Bononi A  Bonora M  Duszynski J  Bernardi R  Rizzuto R  Tacchetti C  Pinton P  Pandolfi PP 《Science (New York, N.Y.)》2010,330(6008):1247-1251

The promyelocytic leukemia (PML) tumor suppressor is a pleiotropic modulator of apoptosis. However, the molecular basis for such a diverse proapoptotic role is currently unknown. We show that extranuclear Pml was specifically enriched at the endoplasmic reticulum (ER) and at the mitochondria-associated membranes, signaling domains involved in ER-to-mitochondria calcium ion (Ca(2+)) transport and in induction of apoptosis. We found Pml in complexes of large molecular size with the inositol 1,4,5-trisphosphate receptor (IP(3)R), protein kinase Akt, and protein phosphatase 2a (PP2a). Pml was essential for Akt- and PP2a-dependent modulation of IP(3)R phosphorylation and in turn for IP(3)R-mediated Ca(2+) release from ER. Our findings provide a mechanistic explanation for the pleiotropic role of Pml in apoptosis and identify a pharmacological target for the modulation of Ca(2+) signals.  相似文献   

Iron content in vegetative and reproductive organs of nectarine trees in calcareous soils during the development of chlorosis     

Moreno Toselli  Bruno Marangoni  Massimo Tagliavini   《European Journal of Agronomy》2000,13(4):279-286

We investigated for 2 years (1995–1996) the time course development of chlorosis and the variation of iron (Fe) content in vegetative and reproductive organs in two nectarine orchards planted with cv Spring Red and cv Stark Redgold on calcareous soils of the Po valley (Italy) with the final aim to evaluate possible tools for the early prognosis of Fe chlorosis and a more efficient fertilization management. Due to the withdrawal of Fe supply, floral Fe concentration significantly decreased in 1996 as compared with 1995 in cv Spring Red, but not in Stark Redgold. Correlation coefficients between Fe and chlorophyll (Chl) from the same leaves were always higher when Fe was considered as amount present per leaf or per unit of leaf area than as leaf dry weight. The fact that chlorotic and green leaves had similar Fe concentration could be explained by an overestimate of Fe in the chlorotic leaf as a consequence of a reduction of its size. However, the decrease of Chl concentration between 60 and 120 days after full bloom (DAFB) occurred while leaf Fe content generally increased, indicating that even during chlorosis development leaves were supplied with some iron. We therefore suggest that the development of chlorosis was associated with an inactivation of Fe in the leaf apoplast. In 1995, regardless the cultivar, floral Fe concentration and leaf Chl were never correlated. In 1996 floral Fe concentration was linearly related to leaf Chl recorded 60 and 120 DAFB in cv Spring Red only. Floral Fe concentration at full bloom 1996, regardless the variety, was linearly related to leaf Chl determined in spring of the previous year, suggesting that flower Fe concentration might be used for assessing the storage of iron during the previous season.  相似文献   

Comparison among recognized and non-recognized Phaseolus vulgaris L. landraces as traditional agro-food products of the Campania region (Italy)     

Angela R. Piergiovanni  Domenica Villecco  Lucia Lioi  Massimo Zaccardelli 《Genetic Resources and Crop Evolution》2015,62(7):1009-1019

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Characterization of the in vitro core surface proteome of Mycoplasma mycoides subsp. mycoides, the causative agent of contagious bovine pleuropneumonia     

Ivanka Krasteva  Anne Liljander  Anne Fischer  David G.E. Smith  Neil F. Inglis  Massimo Scacchia  Attilio Pini  Joerg Jores  Flavio Sacchini 《Veterinary microbiology》2014

Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides (Mmm) is a severe cattle disease, present in many countries in sub-Saharan Africa. The development of improved diagnostic tests and vaccines for CBPP control remains a research priority. Polyacrylamide gel electrophoresis and mass spectrometry were used to characterize the Triton X-114 soluble proteome of nine Mmm strains isolated from Europe or Africa. Of a total of 250 proteins detected, 67 were present in all strains investigated. Of these, 44 were predicted to be lipoproteins or cytoplasmic membrane-associated proteins and are thus likely to be members of the core in vitro surface membrane-associated proteome of Mmm. Moreover, the presence of all identified proteins in other ruminant Mycoplasma pathogens were investigated. Two proteins of the core proteome were identified only in other cattle pathogens of the genus Mycoplasma pointing towards a role in host–pathogen interactions. The data generated will facilitate the identification and prioritization of candidate Mycoplasma antigens for improved control measures, as it is likely that surface-exposed membrane proteins will include those that are involved in host–pathogen interactions.  相似文献