Ixodid ticks on dogs belonging to people in rural communities and villages in Maputo Province, Mozambique     

De Matos C  Sitoe C  Neves L  Bryson NR  Horak IG 《The Onderstepoort journal of veterinary research》2008,75(2):103-108

The species composition and geographic distribution of ixodid ticks infesting domestic dogs owned by people in rural communities and villages in Maputo Province was established by collecting ticks from dogs at each of 27 localities spread throughout the province. Ticks were collected from a total of 132 dogs, and nine species belonging to four genera were identified. One dog was infested with six species, three with five and 13 with four species. Haemaphysalis elliptica followed by Rhipicephalus simus were present on dogs at most localities, and their geographic distribution in Maputo Province has been mapped for the first time.  相似文献   

Estrogens metabolism associated with polymorphisms: influence of COMT G482a genotype on age at onset of canine mammary tumors     

Dias Pereira P  Lopes CC  Matos AJ  Pinto D  Gärtner F  Lopes C  Medeiros R 《Veterinary pathology》2008,45(2):124-130

Catechol-O-methyltransferase (COMT) is an important enzyme participating in inactivation of carcinogenic oestrogen metabolites. In humans there is a single nucleotide polymorphism in COMT gene (COMT val158met) that has been associated with an increased risk for developing breast cancer. In dogs, there is a single nucleotide polymorphism in COMT gene (G482A), but its relation with mammary carcinogenesis has never been investigated. The aim of this study was to focus on the evaluation of such polymorphism as a risk factor for the development of mammary tumors in bitches and on the analysis of its relationship with some clinicopathologic features (dog's age and weight, number and histologic type of the lesions, lymph node metastasis) of canine mammary neoplasms. A case-control study was conducted analyzing 90 bitches with mammary tumors and 84 bitches without evidence of neoplastic disease. The COMT G482A polymorphism was analyzed by PCR-RFLP. We found a protective effect of the polymorphism in age of onset of mammary tumors, although we could not establish a significant association between COMT genotype and other clinicopathologic parameters nor with mammary tumor risk overall. Animals carrying the variant allele have a threefold likelihood of developing mammary tumors after 9 years of age in comparison with noncarriers. The Kaplan-Meier method revealed significant differences in the waiting time for onset of malignant disease for A allele carrier (12.46 years) and noncarrier (11.13 years) animals. This investigation constitutes the first case-control study designed to assess the relationship between polymorphic genes and mammary tumor risk in dogs. Our results point to the combined effect of COMT genotype with other genetic and/or environmental risk factors as important key factors for mammary tumor etiopathogenesis.  相似文献   

Supplemented Morus nigra extract‐based medium associated with FSH enables the survival and growth of isolated ovine secondary ovarian follicles          下载免费PDF全文

AYP Cavalcante  TLBG Lins  JMS Santos  VRP Barros  APO Monte  RS Barberino  JRGS Almeida  MHT Matos 《Reproduction in domestic animals》2018,53(2):423-432

The effects of Morus nigra ethanolic extract, without or with addition of supplements associated or not with FSH, on in vitro culture of ovine secondary follicles were evaluated. In experiment 1, isolated secondary follicles were cultured for 12 days in α‐MEM alone (control) or in different concentrations of M. nigra extract (MN 0.025; 0.05 or 0.1 mg/ml). In experiment 2, culture media were α‐MEM supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine and ascorbic acid (α‐MEM⁺) or this medium associated with FSH (α‐MEM⁺ + FSH), or 0.1 mg/ml M. nigra without supplements (MN 0.1) or supplemented (MN 0.1⁺) without or with FSH (MN 0.1⁺ + FSH). In experiment 1, 0.1 mg/ml M. nigra showed the highest percentages (p < .05) of normal follicles and fully grown oocytes, besides a higher follicular diameter than α‐MEM and other M. nigra extract concentrations. Moreover, MN 0.1 showed lower (p < .05) glutathione (GSH) levels and similar (p > .05) mitochondrial activity compared to α‐MEM. In experiment 2, MN 0.1⁺ + FSH showed similar results (p > .05) to α‐MEM⁺ + FSH for all parameters evaluated, except for the daily growth rate, which was higher (p < .05) in MN 0.1⁺ + FSH. The GSH levels were higher in MEM⁺ than all treatments. In addition, oocytes from follicles cultured in MN 0.1⁺ + FSH showed ability to resume meiosis. In conclusion, M. nigra extract (0.1 mg/ml) added by supplements and FSH can be an efficient medium for ovine secondary follicle development.  相似文献   

Automated identification of intensive animal production locations from aerial photography          下载免费PDF全文

KJ Sheffield  JC Hunnam  TN Cuzner  EM Morse‐McNabb  SM Sloan  J Nunan  J Smith  W Harvey  H Lewis 《Australian veterinary journal》2018,96(9):323-331

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Protein Localization of Epidermal Growth Factor in Sheep Ovaries and Improvement of Follicle Survival and Antrum Formation In Vitro     

LP Santos  VRP Barros  AYP Cavalcante  VG Menezes  TJS Macedo  JMS Santos  VR Araújo  MAA Queiroz  MHT Matos 《Reproduction in domestic animals》2014,49(5):783-789

The aims of this study were to characterize EGF protein expression in ovine ovaries and to verify the effect of EGF on the in vitro development of isolated pre‐antral follicles. After collection, ovarian tissue was fixed for immunohistochemical analysis. Additional pairs of ovaries were collected, and secondary follicles were cultured for 18 days in α‐MEM⁺ (control) alone or supplemented with EGF (1, 10 or 50 ng/ml). The immunostaining for EGF was observed in oocytes from pre‐antral and antral follicles, in granulosa cells of primary and secondary follicles, as well as in cumulus and mural cells of antral follicles. After 18 days, the results showed that treatment with 50 ng/ml EGF significantly increased the percentage of morphologically normal follicles compared with the control group (α‐MEM⁺) and significantly reduced the precocious extrusion of oocytes and increased the percentage of antral follicles compared with the control and 1 ng/ml EGF. All the treatments induced a progressive and significant increase of the follicular diameter throughout the period of culture. However, there were no significant differences in follicular diameter or in the daily growth rate among treatments. In conclusion, this study demonstrated the presence of EGF in ovine ovaries. Moreover, 50 ng/ml EGF increased the percentage of normal follicles and improved antrum formation in isolated ovine follicles after 18 days of in vitro culture.  相似文献   

Experimental integrated aquaculture of fish and red seaweeds in Northern Portugal     

J. Matos  A. Rodrigues  I. Sousa Pinto 《Aquaculture (Amsterdam, Netherlands)》2006,252(1):31-42

Three potentially valuable red seaweeds, Chondrus crispus Stackhouse, Gracilaria bursa pastoris (S.G. Gmelin) P.C. Silva and Palmaria palmata (L.) O. Kuntze, collected in northern Portugal, were cultivated using the nutrient-rich effluents from a local turbot (Scophthalmus maximus Linnaeus) and sea bass (Dicentrarchus labrax Linnaeus) farm. The algae were cultivated in a two level cascade system. Several arrangements of the cascade system, stocking densities (3, 5, 7 and 8 kg m^− 2) and water fluxes (140 and 325 l h^− 1) were tested to optimize biomass yield and nitrogen uptake rate and efficiency. The yield and the total ammonium nitrogen (TAN) uptake of the three species were highly seasonal. Palmaria could not survive culture conditions during the summer when water temperature was above 21 °C. In the spring, Palmaria had an average yield of 40.2 (± 12.80) g DW m^− 2 day^− 1 and a nitrogen uptake efficiency (NUE) of 41.0% (± 17.26%). NUE expresses, in percentage, the average reduction in TAN concentration between the inflows and the outflows of the tanks. Chondrus performed better in summer with an average yield of 37.0 (± 11.10) g DW m^− 2 day^− 1 and removing 41.3% (± 17.32%) of nitrogen. Gracilaria grew year round, but also performed better during spring/summer, producing an average of 29.1 (± 2.90) g DW m^− 2 day^− 1, and only 7.3 (± 5.08) g DW m^− 2 day^− 1 during autumn. Yield of C. crispus did not differ significantly when grown at two different stocking densities (5 kg m^− 2 and 8 kg m^− 2). On the other hand, Gracilaria had significantly higher yields at 5 than at 7 kg m^− 2. Better NUE, on average 76.7% (± 22.13%), was also obtained with 5 kg m^− 2 stocking density and only 63.8% (± 24.62%) with 7 kg m^− 2. The yield of Gracilaria increased significantly with the increase of water flux from 140 to 325 l h^− 1 and more nitrogen was removed from the water. However, NUE decreased from 48.4% to 33.4% at 140 and 325 l h^− 1, respectively. Biofiltration was highly improved by a cascade system with a NUE as high as 83.5%.  相似文献   

Assessment of the accuracy of different standard methods for determining reservoir capacity and sedimentation     

Marcus Vinicius Estigoni  Artur José Soares Matos  Frederico Fabio Mauad 《Journal of Soils and Sediments》2014,14(7):1224-1234

Purpose

Determination of reservoir volume has been used in the calculation of sedimentation, hydrodynamic modelling, and dam safety assessment, among others, and the method used as well as its associated error play an important role in the quality of these studies. This study assessed the accuracy of reservoir volume calculations based on different standards for the definition of the spacing between bathymetric range lines. A technique for terrain modelling and a method for determining the survey accuracy are also proposed.

Materials and methods

The reference reservoir volume was based on highly detailed bathymetry (10 m resolution). Seven different spacings were selected, which generated 99 separate bathymetric data sets. For each data set, the volume was calculated based on a digital terrain model according to the usual model (triangular irregular network (TIN)) and according to the insertion of mesh points (IMP) method. This was then compared with the reference volume, and the accuracy of each survey was determined.

Results and discussion

Utilization of a highly detailed and reliable bathymetric survey allowed for the assessment of the associated error in each of the bathymetric data sets, as well as of the spacing standards analyzed. The accuracy of the standards analyzed using TIN modeling ranged from 0.31% to 14.40% (30 and 300 m, respectively). The high errors are attributed to the limitation of the TIN in representing regions near shores, producing incorrect shallow depth readings, resulting in a lower calculated volume than the real volume. IMP modeling exhibited great improvement in performance over the standard TIN (i.e., for 300 m accuracy of 5.51% for IMP and 14.40% for standard TIN), and in none of the cases were the generated volumes greater than the reference volume.

Conclusions

The IMP method proved to be consistent, improving quality of the data while reducing effort in field work. The low accuracies found showed that even surveys following the widely accepted standards contain errors of a magnitude that cannot be ignored. Thus, knowing and evaluating the survey accuracy is of fundamental importance. Definition of standard spacing for reservoirs proved to be unfeasible. This must be defined on a case-to-case basis, considering the error acceptable for the purpose of the survey.  相似文献   

Phosphorus pools in soil after land conversion from silvopasture to arable and grassland use          下载免费PDF全文

Anna Slazak  Dirk Freese  Eduardo S. Matos  Seth Nii‐Annang  Reinhard F. Hüttl 《植物养料与土壤学杂志》2014,177(2):159-167

Differences in soil P among silvopasture, grassland, and arable lands have been well established. Nevertheless, most of the reports compare soil properties under long‐term sites. Thus, there exists little information on the effect of the conversion of silvopasture to arable or grassland use on soil P pools. The objective of the study was to determine the impact of converting silvopasture system (SP) into arable cropping and grassland system on the distribution of P pools and potential P bioavailability. We compared the following systems: SP system, SP converted to arable cropland (SP‐AL), SP converted to grassland (SP‐GL), and for comparative purposes, a long‐term arable cropland (AL). The P fractionation was performed by a sequential extraction scheme, using acid and alkaline extractants on samples collected from the 0–10 and 10–20 cm soil layers. It was assumed that the large variations in soil‐P fractionations are caused by the different management practices associated with land conversion. The results of P fractionation showed a dominance of calcium‐bound P, HCl‐extractable Pi constituted up to 36% of the soil total P (TP). However, the type of land use did not affect this P fraction. On the other hand, the reduction in labile‐P_i and NaOH‐P_i fractions observed at the SP‐AL site may have led to the decline in readily available P. The soil total organic P (TP_o) content was 8% and 17% lower at SP‐AL compared to SP and SP‐GL site, respectively. Labile organic‐P (labile‐P_o) content was markedly higher at SP site compared to arable soils, and was ≈ 10% of TP_o. The NaOH‐P_o constituted the highest fraction of the organic‐P pool (55%–79% of TP_o) across all the study systems, and was positively correlated with TP_o (p < 0.01). The study indicates that conversion of SP system in temperate regions to arable cropping with conventional tillage seems to result in the reduction of P availability compared to SP, indicating SP as an important land‐use practice.  相似文献   

High‐resolution mapping of soil phosphorus concentration in agricultural landscapes with readily available or detailed survey data          下载免费PDF全文

M. Matos‐Moreira  B. Lemercier  R. Dupas  D. Michot  V. Viaud  N. Akkal‐Corfini  B. Louis  C. Gascuel‐Odoux 《European Journal of Soil Science》2017,68(3):281-294

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Dietary inclusion of tomato pomace improves meat oxidative stability of young pigs          下载免费PDF全文

C. S. Correia  C. M. Alfaia  M. S. Madeira  P. A. Lopes  T. J. S. Matos  L. F. Cunha  J. A. M. Prates  J. P. B. Freire 《Journal of animal physiology and animal nutrition》2017,101(6):1215-1226

The aim of this study was to assess the nutritional effect of tomato pomace, in combination with two distinct fat sources (lard or soya bean oil), on longissimus lumborum muscle, subcutaneous fat and liver of young pigs. Forty male pigs of 4 weeks old were randomly assigned to four dietary treatments using two fibre sources (wheat bran or 5.0% tomato pomace) and two fat sources (lard or soya bean oil), in a 2 × 2 factorial design for a 5‐week trial. Tomato pomace did not improve colour, fatty acid profile, cholesterol content or lipid oxidation, nor did it yield detectable levels in lycopene or β‐carotene in the meat (p > 0.05). However, a positive increase in α‐tocopherol content of meat and liver was observed in pigs fed tomato pomace (p < 0.05). Compared with lard, soya bean oil increased MUFA and PUFA proportions and decreased the percentage of SFA (p < 0.05). Our results indicate that 5.0% of tomato pomace increases pork oxidative stability and that 5.0% of soya bean oil, compared with lard, improves its nutritional quality.  相似文献