Irrigation scheduling for potatoes in Michigan     

Maurice L. Vitosh 《American Journal of Potato Research》1984,61(4):205-213

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Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi   总被引：1，自引：0，他引：1  

Fateh Toumi  Lieven Waeyenberge  Nicole Viaene  Amer Dababat  Julie M. Nicol  Francis Ogbonnaya  Maurice Moens 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(3):613-624

Twelve Heterodera species are of major economic significance in wheat and barley. Of these, H. avenae, H. filipjevi and H. latipons are among the most important ones, and sometimes coexist. The identification of Heterodera species using morphological characteristics is time consuming, requires specialized skill and can be imprecise, especially when they occur mixed in field populations. Molecular techniques can provide a more accurate way for nematode identification. This study reports the results of experiments targeting the mitochondrial cytochrome oxidase subunit 1 (COI) gene to develop species-specific primers that could be used for the identification of H. avenae and H. filipjevi. The COI gene of 9 Heterodera spp. and Punctodera punctata was partially sequenced and the resultant sequences were aligned to find unique sites suitable for the design of primers. The alignment showed variability between H. avenae, H. filipjevi and other Heterodera species. Two sets of species-specific primers were identified for the identification of both species and the conditions for their use in PCR were optimised. The specificity of the designed primers was checked by comparison with one population of P. punctata and populations of 14 other Heterodera species, nine populations of H. avenae and 10 populations of H. filipjevi originating from different countries. To test the sensitivity, the PCR was run with DNA extracted from five second-stage juveniles (J2) of H. avenae or five J2 of H. filipjevi mixed with DNA extracted from varying numbers of J2 of H. latipons. It was possible to detect as few as five J2 of H. avenae or H. filipjevi among 100 J2 of H. latipons. The two primers sets allow the detection of H. avenae and H. filipjevi where they occur in mixed populations with other Heterodera spp.  相似文献   

Thermal barrier coatings for gas-turbine engine applications   总被引：6，自引：0，他引：6  

Padture NP  Gell M  Jordan EH 《Science (New York, N.Y.)》2002,296(5566):280-284

Hundreds of different types of coatings are used to protect a variety of structural engineering materials from corrosion, wear, and erosion, and to provide lubrication and thermal insulation. Of all these, thermal barrier coatings (TBCs) have the most complex structure and must operate in the most demanding high-temperature environment of aircraft and industrial gas-turbine engines. TBCs, which comprise metal and ceramic multilayers, insulate turbine and combustor engine components from the hot gas stream, and improve the durability and energy efficiency of these engines. Improvements in TBCs will require a better understanding of the complex changes in their structure and properties that occur under operating conditions that lead to their failure. The structure, properties, and failure mechanisms of TBCs are herein reviewed, together with a discussion of current limitations and future opportunities.  相似文献   

Global distribution of neutrons from Mars: results from Mars odyssey     

Feldman WC  Boynton WV  Tokar RL  Prettyman TH  Gasnault O  Squyres SW  Elphic RC  Lawrence DJ  Lawson SL  Maurice S  McKinney GW  Moore KR  Reedy RC 《Science (New York, N.Y.)》2002,297(5578):75-78

Global distributions of thermal, epithermal, and fast neutron fluxes have been mapped during late southern summer/northern winter using the Mars Odyssey Neutron Spectrometer. These fluxes are selectively sensitive to the vertical and lateral spatial distributions of H and CO2 in the uppermost meter of the martian surface. Poleward of +/-60 degrees latitude is terrain rich in hydrogen, probably H2O ice buried beneath tens of centimeter-thick hydrogen-poor soil. The central portion of the north polar cap is covered by a thick CO2 layer, as is the residual south polar cap. Portions of the low to middle latitudes indicate subsurface deposits of chemically and/or physically bound H2O and/or OH.  相似文献   

Detecting energy emissions from a rotating black hole     

van Putten MH  Levinson A 《Science (New York, N.Y.)》2002,295(5561):1874-1877

The rotational energy of a black hole surrounded by a torus is released through several channels. We have determined that a minor fraction of the energy is released in baryon-poor outflows from a differentially rotating open magnetic flux tube, and a major fraction of about eta/2 is released in gravitational radiation by the torus with angular velocity eta similar 0.2 to 0.5 relative to that of the black hole. We associate the energy emitted in baryon-poor outflows with gamma-ray bursts. The remaining fraction is released in torus winds, thermal emissions, and (conceivably) megaelectron-volt neutrino emissions. The emitted gravitational radiation can be detected by gravitational wave experiments and provides a method for identifying Kerr black holes in the Universe.  相似文献   

Therapeutic efficacy of topical hydrocortisone aceponate in experimental flea-allergy dermatitis in dogs   总被引：1，自引：1，他引：0  

S Bonneau  V Skowronski  A Sanquer  L Maynard  HM Eun 《Australian veterinary journal》2009,87(7):287-291

Objective   To evaluate the treatment efficacy of a topical spray containing hydrocortisone aceponate (HCA) on dogs with flea-allergy dermatitis (FAD).
Design   A controlled clinical study was conducted on dogs with experimentally induced FAD. Sixteen laboratory beagles with mild to moderate clinical signs were divided into two groups. The test group received HCA by topical spray once daily for 7 days, while the control group did not. Pruritic events (time and frequency) were videotaped and then scored. Clinical signs (erythema, papules, excoriation and alopecia) present on four anatomical regions were monitored and their severity directly assessed.
Results   After 2 days, pruritus was reduced by 94% in the treatment group and by 24% in the control group (P = 0.014) in cumulative time, and by 86% versus 34% (P = 0.034) in frequency. The HCA spray also resulted in significant improvements in overall clinical signs: 23% versus 0% in the control group (P = 0.0006) on day 3 and 43% versus 15% in the control group (P = 0.0006) on day 7. During the 7-day trial, no drug-related adverse effects were observed.
Conclusions   Topical treatment with HCA showed a rapid and potent antipruritic effect on dogs with FAD. HCA also demonstrated significant overall therapeutic effects on FAD-associated skin lesions.  相似文献   

Goat farming systems in Martinique: management and breeding strategies     

Alexandre G  Leimbacher F  Maurice O  Domarin D  Naves M  Mandonnet N 《Tropical animal health and production》2009,41(4):635-644

To be successful, initiatives to improve farmer's goat production should directly address the needs and objectives of the keepers while promoting rational use of local genetic resources. A survey was carried out to implement a genetic policy governing meat goat farming in Martinique (11400 heads and 33400 ha arable land). The questionnaire comprised a total of 27 items with 306 modalities, and included questions on farm structure, crop and animal productions, management of feeding, reproduction and health control. The sample consisted of 33 farmers with 644 ha and 2680 goats (1286 does and 52 bucks), 97% of does in the studied sample were crossbred, 56% of bucks were" imported" breeds (Boer or Anglo-Nubian). The number of goats per farm varied from 16 to 582. The feeding system was predominantly grazing, according to a rotation (55% of cases) or continuous grazing system (42%). On 62% of farms, the males remained with the females permanently, also 83% of farmers did not resort to methods of controlled-mating. The first criteria used for choosing animals (80 to 90% of answers) of both sex, were development and conformation. Assuming that adaptive together with productive traits are important in tropical zones, it is advisable to better define the maternal lineage of the local livestock (presently very sparse records), to improve reproduction management and culling strategies (poor and inadequate management practices do not support any genetic improvement programme), and to guide the farmers in their decisions by employing concerted interprofessional actions (choice of meat breed, market studies).  相似文献   

Ring tests to evaluate the performance of Porcine circovirus-2 (PCV-2) polymerase chain reaction (PCR) assays used in North American diagnostic laboratories          下载免费PDF全文

John C.S. Harding  Crissie Baker  Carrie Rhodes  Kathleen A. McIntosh    Martin Bonneau 《Canadian journal of veterinary research》2009,73(1):7-14

Two laboratory studies involving 11 laboratories were undertaken to assess the performance of North American Porcine circovirus-2 (PCV-2) polymerase chain reaction (PCR) assays. Laboratories received identical submissions containing randomly coded positive and negative control samples, and serially diluted PCV-2-spiked samples. In study 1 and 2, respectively, spiked samples contained measured amounts of PCV-2 virus or DNA. All but 1 assay detected DNA in the most concentrated spiked sample. There were no statistical differences in the proportion of positive or negative samples reported by quantitative (n = 7) versus non-quantitative (n = 6) assays. Across both studies, the false positive rate was 17% (4 out of 23), and 17% (2 out of 12) of assays cross-reacted with PCV-1. The most sensitive assay detected PCV-2 DNA levels about 100 000 times lower the least sensitive assay. This study demonstrated that the PCR assays available in North American diagnostic labs vary considerably in their detection limits and quantification.  相似文献   

Effects of Leachate Irrigation on Landfill Vegetation and Subsequent Methane Emissions     

Maurice  C.  Ettala  M.  Lagerkvist  A. 《Water, air, and soil pollution》1999,113(1-4):203-216

Short-rotation tree forests are irrigated with landfill leachate to reduce both leachate volume and nutrient content. It is of interest both for leachate treatment and energy recovery to optimise the productivity of such plantations. This study's aim was to investigate the effects of irrigation on soil quality, tree growth and on emissions of landfill gas (LFG) produced in the wastes. Soils irrigated with leachate had a higher average nutrient and organic matter contents, and a lower dry solids content. Larger trees occur on plots irrigated with leachate, presumably due to the positive effect of water and nutrient supply. The willows used in this experiment seemed to be tolerant of high carbon dioxide concentrations, as no statistically effect arising from LFG emissions could be linked to tree growth. Methane oxidation levels between 50 and 950 mol m^-2 yr^-1 were observed. The positive correlation between soil methane oxidation capacity and tree presence is an interesting perspective on reduction of methane emissions by landfill's top cover vegetation type. Optimising methane oxidation using vegetation as a ‘cover crop’ could reduce the amount of methane discharged into the atmosphere.  相似文献   

Molecular Characterisation of 18 Pratylenchus Species Using rDNA Restriction Fragment Length Polymorphism     

LievenWaeyenberge  AlexanderRyss  MauriceMoens  JorgePinochet  ThierryC.Vrain 《云南农业大学学报(自然科学版)》1999,14(Z1):128-128

 rDNA RFLP is a reliable method for identification purposes, and a powerful tool to analyse genetic variation in nematodes.  相似文献