Growth,behavioural, serum biochemical and morphological changes in female rabbits fed high-fat diet     

Jiahao Shao  Jie Wang  Yanhong Li  Mauricio A. Elzo  Tao Tang  Tianfu Lai  Yuan Ma  Mingchuan Gan  Li Wang  Xianbo Jia  Songjia Lai 《Journal of animal physiology and animal nutrition》2021,105(2):345-353

This study aimed to determine whether high-fat diet (HFD) could cause growth, behavioural, biochemical and morphological changes in young female rabbits. Thirty-six female rabbits were randomly divided into two groups fed with either a high-fat diet (HFD) or a standard normal diet (SND) for 5 weeks. Growth and behavioural changes were recorded during the 5-week feeding period. Tissue samples, including blood and adipose tissue, were obtained after slaughter. HFD rabbits weighed more by the end of the feeding period, had a higher percent body weight and adipose tissue weight change and had longer body and bust lengths than SND rabbits. HFD rabbits significantly reduced their feed intake and feeding frequency during the fourth and fifth weeks. HFD rabbits also showed lower frequency of drinking and resting and increased stereotypical behaviour. Besides, HFD rabbits showed significant physiological abnormalities. HFD rabbits had higher serum cholesterol (TC) and triglycerides (TG) levels than SND rabbits at the end of the feeding period, and higher free fatty acid (FFA) levels than rabbits in the SND group after the third week of feeding. Serum thyroxine (T4) increased significantly in week 2 and week 5 and triiodothyronine (T3) increased significantly in week four. However, there was no significant change in serum glucose (GLU) and insulin (INS) levels. Additionally, HFD reduced the area and diameter of perirenal and subcutaneous fat cells and increased their density. Our findings suggest that HFD rabbits had higher weight gains, accumulation of fat, and more behavioural changes than SND rabbits. Although high levels of fat in the diet had a low impact on hyperglycaemia, it could lead to hyperlipidemia and hyperthyroidism. Our results also suggest that sustained HFD may cause the proliferation of adipocytes in young female rabbits.  相似文献   

Rain-induced removal of copper from apple leaves: Influence of rain properties and tank-mix adjuvants on deposit characteristics at the micro scale     

Mauricio Hunsche  Anastasia Alexeenko  Lutz DamerowGeorg Noga 《Crop Protection》2011,30(4):495-501

Application of copper fungicides is an integrative component in the control of apple scab. As shown in the past, tank-mix adjuvants might reduce the rain-induced removal of active ingredients. The aims of this study were a) to characterize the impact of defined rain amount on the removal of Cu-oxychloride (Cu-Ox) and Cu-hydroxide (Cu-Hyd) from apple seedling leaves, b) to examine the influence of ethoxylated seed oil adjuvants on deposit properties at the micro scale and their possible influence in reducing the fungicide erosion from the leaves, and finally c) to evaluate the influence of previous adjuvant application on the rainfastness of copper ions. Losses of Cu-Hyd and Cu-Ox from seedling leaves exposed to 5 mm heavy rain reached almost 80% of the original Cu load. Wash-off extent of Cu-Ox could be reduced by using the rapeseed oil ethoxylate RSO 5 (47%), the linseed oil ethoxylate LSO 10 (47%), or the soybean oil ethoxylate SBO 10 (40%). However, in case of Cu-Hyd, none of the adjuvants was able to increase the tenacity of the copper on the leaves. Rainfall removed the bulky deposits and induced changes in the typical shape of the crystals. Furthermore, a reorganization of the spatial distribution of the remaining active ingredient (a.i.) was induced, which was very often embedded in a thin layer of adjuvants. In general, the adjuvants had a significant impact on droplet spread, whereas the area effectively covered with the a.i. was affected to a lesser extent. For both Cu-Hyd and Cu-Ox rewetting of the original deposit under controlled conditions resulted in an increased spread area but reduced the area effectively covered with Cu. Our results showed no clear relationship between the area covered by Cu ions and their adhesion to the surface, i.e. rainfastness. Finally, our studies indicated that previous spray treatments might influence the rain-induced removal of copper.  相似文献   

Robust fitting of fluorescence spectra for pre-symptomatic wheat leaf rust detection with Support Vector Machines   总被引：2，自引：0，他引：2  

Christoph Römer  Kathrin BürlingMauricio Hunsche  Till RumpfGeorg Noga  Lutz Plümer 《Computers and Electronics in Agriculture》2011,79(2):180-188

Early recognition of pathogen infection is of great relevance in precision plant protection. Pre-symptomatic disease detection is of particular interest. By use of a laserfluoroscope, UV-light induced fluorescence data were collected from healthy and with leaf rust inoculated wheat leaves of the susceptible cultivar Ritmo 2-4 days after inoculation under controlled conditions. In order to evaluate pathogen impact on fluorescence spectra 215 wavelengths in the range of 370-800 nm were recorded. The medians of fluorescence signatures suggest that inoculated leaves may be separated from healthy ones, but high-frequency oscillations and individual reactions of leaves indicate that separability is difficult to achieve. The misbalance between the high number of measured wavelengths and the low number of training examples induces a high overfitting risk. For a pre-symptomatic pathogen identification a small number of robust features was desired which comprise most of the information relevant for the given classification task. Instead of choosing only the most relevant wavelengths, the coefficients of polynomials fitting the spectra were used for classification. They specify the global curve characteristics. Piecewise fitting by polynomials of fourth order led to high classification accuracy. Support Vector Machines were used for classification. Cross validation demonstrated that the achieved classification accuracy reached 93%. This result could be attained on the second day after inoculation, before any visible symptoms appeared. The described method is of general interest for pre-symptomatic pathogen detection based on fluorescence spectra.  相似文献   

The native South American crayfishes (Crustacea,Parastacidae): state of knowledge and conservation status          下载免费PDF全文

Mauricio Pereira Almerão  Erich Rudolph  Catherine Souty‐Grosset  Keith Crandall  Ludwig Buckup  Julien Amouret  Ana Verdi  Sandro Santos  Paula Beatriz De Araujo 《水产资源保护:海洋与淡水生态系统》2015,25(2):288-301

Assessment of the potential role of Streptomyces strains in the revegetation of semiarid sites: the relative incidence of strain origin and plantation site on plant performance and soil quality indicators     

Carmen Mengual  Mauricio Schoebitz  Fuensanta Caravaca  Antonio Roldán 《Biology and Fertility of Soils》2016,52(1):53-64

  相似文献   

Common Bean Leaves as a Source of Dietary Iron: Functional Test in an Iron-Deficient Rat Model     

Mauricio Martínez-Zavala  María Alejandra Mora-Avilés  Miriam Aracely Anaya-Loyola  Horacio Guzmán-Maldonado  Araceli Aguilera-Barreyro  Alejandro Blanco-Labra  Teresa García-Gasca 《Plant foods for human nutrition (Dordrecht, Netherlands)》2016,71(3):259-264

  相似文献   

Natural Bagaza virus infection in game birds in southern Spain     

Gamino V  Gutierrez-Guzman AV  Fernández de Mera IG  Ortíz JA  Durán-Martin M  De la Fuente J  Gortazar C  Höfle U 《Veterinary research》2012,43(1):65

ABSTRACT: In late summer 2010 a mosquito born flavivirus not previously reported in Europe called Bagaza virus (BAGV) caused high mortality in red-legged partridges (Alectoris rufa) and ring-necked pheasants (Phasianus colchicus). We studied clinical findings, lesions and viral antigen distribution in naturally BAGV infected game birds in order to understand the apparently higher impact on red-legged partridges. The disease induced neurologic signs in the two galliform species and, to a lesser extent, in common wood pigeons (Columba palumbus). In red-legged partridges infection by BAGV caused severe haemosiderosis in the liver and spleen that was absent in pheasants and less evident in common wood pigeons. Also, BAGV antigen was present in vascular endothelium in multiple organs in red-legged partridges, and in the spleen in common wood pigeons, while in ring-necked pheasants it was only detected in neurons and glial cells in the brain. These findings indicate tropism of BAGV for endothelial cells and a severe haemolytic process in red-legged partridges in addition to the central nervous lesions that were found in all three species.  相似文献   

Susceptibility of Trypanosoma evansi to propolis extract in vitro and in experimentally infected rats     

Gressler LT  Da Silva AS  Machado G  Rosa LD  Dorneles F  Gressler LT  Oliveira MS  Zanette RA  de Vargas AC  Monteiro SG 《Research in veterinary science》2012,93(3):1314-1317

Current therapy of Trypanosoma evansi infections is not effective for the vast majority of animals with relapsing parasitemia and clinical signs. Recently, attention is being focused on the antiparasitic activity of propolis. This study evaluated the susceptibility of T. evansi to propolis extract in vitro and in vivo. A dose-dependent trypanocidal activity of propolis extract was observed in vitro. All trypomastigotes were killed 1h after incubation with 10μgmL(-1) of the extract. In vivo, the concentrations of 100, 200, 300 and 400mgkg(-1) administered orally for 10 consecutive days showed no curative effect, and the rats died from the disease. However, rats treated with the two highest concentrations of propolis extract showed higher longevity than the other groups. Based on these data, we concluded that T. evansi is susceptible to propolis in vitro. Despite the lack of curative efficacy observed in vivo at the concentrations tested, the propolis extract can prolong life in rats infected with the protozoan.  相似文献   

Effects of a urolith prevention diet on urine compositions of glycosaminoglycans, Tamm-Horsfall glycoprotein, and nephrocalcin in cats with calcium oxalate urolithiasis     

Lulich JP  Osborne CA  Carvalho M  Nakagawa Y 《American journal of veterinary research》2012,73(3):447-451

  相似文献   

Plant growth promoting properties of a strain of Enterobacter ludwigii isolated from Lolium perenne rhizosphere     

Mauricio Shoebitz  Claudia M. Ribaudo  Martín A. Pardo  Luigi Ciampi 《Soil biology & biochemistry》2009,41(9):1768-4466

The capability of native bacterial strains isolated from Lolium perenne rhizosphere to behave as plant growth promoting bacteria and /or biocontrol agents was investigated. One strain (BNM 0357) over 13 isolates from the root tips of L. perenne resulted proved to be nitrogenase positive (ARA test) and an IAA producer. Conventional tests and the API 20E diagnostic kit indicated that BNM 0357 behaves to the Enterobacteriaceae family and to the Enterobacter genus. Molecular identification by 16S rRNA sequence analysis indicated that BNM 0357 had the highest similarity to Enterobacter ludwigii (EN-119). Isolate BNM 0357 had the capability to solubilize calcium triphosphate and to antagonize Fusarium solani mycelial growth and spore germination. Strain BNM 0357 also showed the ability to improve the development of the root system of L. perenne. This study disclosed features of E. ludwigii BNM 0357 that deserve further studies aimed at confirming its putative importance as a PGPR.  相似文献