LC/ESI/MS method for the quantitative detection of guazatine residues in cereals     

Dreassi E  Zizzari AT  Zanfini A  Corbini G  Botta M 《Journal of agricultural and food chemistry》2007,55(17):6850-6856

Guazatine is a fungicide used in agriculture to control a wide range of seed-borne diseases of cereals and other vegetable foods. In this work, a LC-ESI-MS method was developed for the quantitative detection of guazatine residues in maize and hard wheat. Quantitative data were determined for the residues of the main diamines, triamines, and tetramines that cover more than 87% of the total contents of the mixture. The mean recoveries from the fortified cereals at 0.050 mg/kg ranged from 81 to 86%, with the coefficients of variation (CVs) ranging from 0.9 to 5.5% (n = 5). At 0.025 mg/kg, the recoveries ranged from 78 to 87%, with the CVs ranging from 0.8 to 6.3% (n = 5). The limits of quantification have been estimated to be 0.010, 0.004, 0.002, 0.002, 0.005, and 0.002 mg/kg, respectively, for GN, GG, GNG, GGN, GGG, and GGGG in maize and hard wheat (S/N ratio >10).  相似文献   

A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris)     

Casiraghi M  Bazzocchi C  Mortarino M  Ottina E  Genchi C 《Veterinary parasitology》2006,141(3-4):368-372

Accurate diagnosis of canine filariosis is essential for choosing correct therapeutic approach. Therefore, reliable methods for discriminating among the different filarial infections in dogs are needed. The authors report simple and highly specific molecular methods that identify the three most common filarial nematodes of European dogs: Dirofilaria immitis, D. repens and Acanthocheilonema (syn. Dipetalonema) reconditum, based on (1) PCR amplifications of mitochondrial DNA (12S rDNA and coxI) with general filarial primers followed by digestion with restriction enzymes that generates band polymorphisms clearly discriminating the three species and (2) PCR amplifications with species-specific primers to support the restriction analysis, in particular in the case of multiple infections.  相似文献   

Plasticity of growth and sylleptic branchiness in two poplar families grown at three sites across Europe     

Marron N  Bastien C  Sabatti M  Taylor G  Ceulemans R 《Tree physiology》2006,26(7):935-946

Two hybrid poplar maternal half-sib families, resulting from controlled crosses of the female parent Populus deltoides 'S9-2' with P. nigra 'Ghoy' and P. trichocarpa 'V24', were grown at three sites: northern Italy, central France and southern England. Juvenile stem growth traits (height, circumference and volume) and sylleptic branchiness (number of branches, density of branches per unit of stem height, percentage of the stem carrying branches and distance of the highest sylleptic branch to the top of the stem) were measured on 1-year-old shoots. Our general objectives were to determine the degree to which the expression of stem growth and syllepsis and the relationships between them are affected by environmental conditions and to evaluate the efficiency of indirect selection for stem growth using branching traits as secondary criteria. The performance of both families differed significantly within and between sites. Pronounced heterosis was observed and highly significant genotype x environment interactions were found for all traits across the sites. Syllepsis showed more marked genetic variation and plasticity than stem growth traits. Relationships between sylleptic branchiness and stem growth depended on environmental conditions. Heritability values at the individual level ranged between 0.09 and 0.59, but genetic gain in stem volume was not significantly improved when selection was based on sylleptic branch characteristics. However, despite strong phenotypic plasticity among sites, genotypic ranking among the sites was relatively stable.  相似文献   

Common Kibra alleles are associated with human memory performance   总被引：1，自引：0，他引：1  

Papassotiropoulos A  Stephan DA  Huentelman MJ  Hoerndli FJ  Craig DW  Pearson JV  Huynh KD  Brunner F  Corneveaux J  Osborne D  Wollmer MA  Aerni A  Coluccia D  Hänggi J  Mondadori CR  Buchmann A  Reiman EM  Caselli RJ  Henke K  de Quervain DJ 《Science (New York, N.Y.)》2006,314(5798):475-478

Human memory is a polygenic trait. We performed a genome-wide screen to identify memory-related gene variants. A genomic locus encoding the brain protein KIBRA was significantly associated with memory performance in three independent, cognitively normal cohorts from Switzerland and the United States. Gene expression studies showed that KIBRA was expressed in memory-related brain structures. Functional magnetic resonance imaging detected KIBRA allele-dependent differences in hippocampal activations during memory retrieval. Evidence from these experiments suggests a role for KIBRA in human memory.  相似文献   

Strigolactones--intriguing biologically active compounds: perspectives for deciphering their biological role and for proposing practical application     

Vurro M  Yoneyama K 《Pest management science》2012,68(5):664-668

Strigolactones are a class of bioactive natural metabolites produced by plant roots and released into the rhizosphere. They were discovered as signals indispensable for the induction of germination of seeds of root parasitic weeds, but since then, interestingly, many other biological, physiological and ecological roles have been described. This has suddenly provoked huge scientific interest in these compounds within different research fields. In this short perspective the attention is focused mainly on the ongoing and future research aimed at deciphering the biological roles of strigolactones that could positively affect, more or less directly, the management of parasitic weeds.  相似文献   

Full season efficacy of moxidectin microsphere sustained release formulation for the prevention of heartworm (Dirofilaria immitis) infection in dogs   总被引：2，自引：0，他引：2  

Genchi C  Rossi L  Cardini G  Kramer LH  Venco L  Casiraghi M  Genchi M  Agostini A 《Veterinary parasitology》2002,110(1-2):85-91

The authors report the efficacy of an injectable, moxidectin sustained release (SR) formulation for the prevention of canine heartworm infection in endemic areas in northern and central Italy. Three field trials were carried out on a total of 324 dogs. Two hundred forty-three dogs were treated with moxidectin SR 6 months apart and 81 dogs (positive controls) with moxidectin tablets given monthly for 5 consecutive months during the risk season each year throughout the study. Results of testing for microfilariae and circulating adult female antigens were negative for all the experimentally treated dogs at the 6, 7, 11 and 19 months after the last injection. No adverse reactions to moxidectin SR were observed but a moderate pain at palpation and swelling (5-6 cm) at the injection site after the first treatment. In the study areas, prevalence of Dirofilaria immitis infection calculated by testing dogs which had no preventive treatment in the previous transmission season ranged from 33 to 63%. This study confirms the efficacy and safety of injectable, moxidectin SR formulation in the prevention of heartworm infection in dogs and demonstrates that the prophylactic efficacy lasts for the full season and strongly suggests that the product gives 1-year protection.  相似文献   

Synthesis and in-vitro antifungal activity of 6-trifluoromethylpyrazolo[3,4-d]pyrimidines     

Tiziana Poli  Chiara B. Vicentini  Vincenzo Brandolini  Augusto C. Veronese  Maurizio Manfrini  Mario Guarneri  Paolo Giori 《Pest management science》1989,25(2):161-169

Thirty 6-trifluoromethylpyrazolo[3,4-d]pyrimidines were synthesised and tested for antifungal activity in vitro against ten phytopathogenic fungi of different taxonomic classes. Six of the compounds had noteworthy activity against Sclerotinia minor, Corticium solani and Phoma betae. The relationships between structure and activity are discussed on the basis of the present and previous studies.  相似文献   

High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MSn) Analysis and Bioactivity Useful for Prevention of “Diabesity” of Allium commutatum Guss     

Loizzo  Monica R.  Tundis  Rosa  Sut  Stefania  Dall’Acqua  Stefano  Ilardi  Vincenzo  Leporini  Mariarosaria  Falco  Tiziana  Sicari  Vincenzo  Bruno  Maurizio 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(1):124-130

Plant Foods for Human Nutrition - This study aimed at evaluating and comparing the chemical profile obtained by HPLC-ESI-MSn analysis, the inhibitory activity of enzymes linked to obesity...  相似文献   

Localization and genotyping of canine papillomavirus in canine inverted papillomas     

Margherita Orlandi  Maurizio Mazzei  Marta Vascellari  Erica Melchiotti  Claudia Zanardello  Ranieri Verin  Francesco Albanese  Fabiano Necci  Luca Pazzini  Giulia Lazzarini  Francesca Abramo 《Journal of veterinary diagnostic investigation》2021,33(6):1069

Numerous canine papillomaviruses (CPVs) have been identified (CPV1–23). CPV1, 2, and 6 have been associated with inverted papillomas (IPs). We retrieved 19 IPs from 3 histopathology archives, and evaluated and scored koilocytes, inclusion bodies, giant keratohyalin granules, cytoplasmic pallor, ballooning degeneration, and parakeratosis. IHC targeting major capsid proteins of PV was performed, and CPV genotyping was achieved by PCR testing. Tissue localization of CPV DNA and RNA was studied by chromogenic and RNAscope in situ hybridization (DNA-CISH, RNA-ISH, respectively). IPs were localized to the limbs (50%), trunk (30%), and head (20%), mainly as single nodules (16 of 19). In 15 of 19 cases, immunopositivity was detected within the nuclei in corneal and subcorneal epidermal layers. PCR revealed CPV1 in 11 IPs and CPV2 DNA in 3 IPs. Overall, 14 of 17 cases were positive by both DNA-CISH and RNA-ISH, in accord with PCR results. A histologic score >5 was always obtained in cases in which the viral etiology was demonstrated by IHC, DNA-CISH, and RNA-ISH. IHC and molecular approaches were useful to ascertain the viral etiology of IPs. Although IHC is the first choice for diagnostic purposes, ISH testing allows identification of PV type and the infection phase. RNA-ISH seems a promising tool to deepen our understanding of the pathogenesis of different PV types in animal species.  相似文献   

A bacterium against the tiger: preliminary evidence of fertility reduction after release of Aedes albopictus males with manipulated Wolbachia infection in an Italian urban area     

Beniamino Caputo  Riccardo Moretti  Mattia Manica  Paola Serini  Elena Lampazzi  Marco Bonanni  Giulia Fabbri  Verena Pichler  Alessandra della Torre  Maurizio Calvitti 《Pest management science》2020,76(4):1324-1332

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