Analysis of Relationship between Bovine Lymphocyte Antigen DRB3.2 Alleles, Somatic Cell Count and milk Traits in Iranian Holstein Population     

M. Pashmi  S. Qanbari  S.A. Ghorashi  A.R. Sharifi  & H. Simianer 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2009,126(4):296-303

The major histocompatibility complex (MHC) is a gene complex closely linked to the vertebrate immune system due to its importance in antigen recognition and immune response to pathogens. To improve our understanding of the MHC and disease resistance in dairy cattle, we gathered 5119 test day records of somatic cell count (SCC) and performance traits of 262 Holstein dairy cows to determine whether the DRB region of the MHC contains alleles that are associated with elevated SCC, milk yield, protein and fat percent of milk. To this purpose, genotyping of animals for DRB3 gene was investigated by polymerase chain reaction‐based restriction fragment length polymorphism (PCR‐RFLP) assay. A two‐step PCR was carried out so as to amplify a 284 base‐pair fragment of exon 2 of the target gene. Second PCR products were treated with three restriction endonuclease enzymes RsaI, BstYI and HaeIII. Twenty‐eight BoLA‐DRB3 alleles were identified including one novel allele (*40). The results in general are in good accordance with allele frequencies of Holstein cattle populations reported by previous studies. Analyses of associations were modeled based on repeated measurement anova and generalized logistic linear methods for production traits and SCC data, respectively. The results of this study showed a significant relationship between the elevated SCC reflecting an increased probability of occurrence to subclinical mastitis and DRB3.2 allele *8 (p < 0.03). The results also revealed significant positive relationships of alleles*22 (p < 0.01) and allele*11 (p < 0.05) with milk fat percent as well as of alleles*24 (p < 0.03) and *22 (p < 0.05) with protein percent. The present study failed to find any association between milk yield and tested alleles. Because of the lack of consistency among results of similar studies, we suggest further investigations to determine the precise nature of these associations with the high polymorphic bovine MHC region to be performed based on haplotypes.  相似文献   

Synergic Effects of Light Salting,Vacuum Packaging,and Onion Extract on the Quality Retention of Refrigerated Rainbow Trout (Oncorhynchus mykiss) Fillets     

Bahareh Shabanpour  Mehdi Zolfaghari 《Journal Of Aquatic Food Product Technology》2013,22(1):84-99

This experiment investigated the synergistic effects of vacuum packaging, light salting, and onion extract on the shelf life of rainbow trout (Oncorhynchus mykiss) fillets stored under refrigeration. The chemical, microbial, and organoleptic changes were monitored for up to 18 days in fillets. Total volatile basic nitrogen (TVB-N) and free fatty acid (FFA) values were evident in the order: control (without salting, vacuum packaging, and extract) > air packaged, salted (APS) > vacuum packaged, salted (VPS) > and vacuum packaged, salted, containing 2% and 4% (v/w) onion extract (2% and 4% VPSOE) throughout the storage period. Thiobarbituric acid values in APS groups were higher than control groups. Data from microbial assay showed that treatment with 2% and 4% VPSOE decreased total viable microbial count (TVC) compared with other treatments. APS, VPS, and VPSOE samples remained acceptable for up to 8, 12, and 15 days, respectively. The most suitable indices for determining shelf life of rainbow trout fillets are TVC.  相似文献   

Salinity threshold value of Quinoa (Chenopodium Quinoa ‎Willd.) at ‎various growth stages and the appropriate ‎irrigation method by saline ‎water     

Parisa Maleki  Saeed Saadat  Forood Sharifi  Farhad Dehghany  Masomeh Salehi 《Communications in Soil Science and Plant Analysis》2018,49(15):1815-1825

Salinity is one of the major agricultural problems in arid and ?semi-arid regions. Considering the variation of plant’s ?sensitivity to salinity during growth, a greenhouse study with completely randomized design? was conducted to determine the relative salinity tolerance of Quinoa (Chenopodium quinoa Willd.) at different growth stages from seedling establishment to maturity (establishment, flowering and seed filling) by evaluating the Salinity Threshold Value (STV). Eight levels of EC_i (i.e., Non-saline, 2, 4, 8, 12, 15, 20, 25 dSm^?1) with four replications and five levels (i.e., Non-saline, 10, 15, 20, 25 dSm^?1) with three triplications were applied at first and two last growth stages, respectively. A ?comparison was performed on some growth and yield parameters of plants irrigated by considering STV (T) and ?plants irrigated permanently by mentioned salinity levels regardless of STV (P) to choose ?which method (P or T) is better at each salinity level. The STV was ?evaluated 8, 20 and 15 dSm^?1 at each growth stage, respectively. Seedling of Quinoa was more sensitive to salinity than the mature plant. Therefore, after establishment Quinoa has the ?feasibility of irrigation by high-saline waters. The (P) ?method was suitable only if the freshwater was available during all growth period of the plant; otherwise at higher salinities irrigation should be performed by considering STV (T method) to minimize the intensity of growth and yield reduction and to prevent yield loss at very high salinities. To achieve this, if high-saline water is available it’s possible to ?use plant propagation techniques or cultivating Quinoa simultaneously with seasonal rainfall.?  相似文献   

Sulfur uptake and translocation in maize (zea mays) grown in a high pH soil treated with elemental sulfur     

Mehdi Karimizarchi  Amin Soltangheisi  Aminuddin Husin  Mohd Khanif Yusop  Radziah Othman 《Journal of plant nutrition》2018,41(14):1798-1806

A glasshouse experiment was conducted to elucidate the influence of elemental sulfur (S) application rates (0, 0.5, 1.0, and 2.0 g S kg^?1 soil) on the release and uptake of S at 0, 20, and 40 days after incubation. Results showed that there was a progressive upward trend in maize leaves, stem, and root S content with application of elemental S. However, maize production followed a nonlinear model. Plants grown in untreated soils suffer from S deficiency and addition of elemental S at a rate of 0.5 g S kg^?1 soil alleviated S deficiency. The decrease in maize performance due to the highest S application rate was not related to S toxicity. The greatest leave, stem, and root productions were obtained at S concentrations of 0.41, 0.58, and 0.2%, respectively. Overall, application of elemental S at a rate of 0.5 g S kg^?1 soil is recommended for maize performance improvement.  相似文献   

Soil moisture index improves models of forage production in central Iran     

Zahra Jaberalansar  Mehdi Bassiri 《Archives of Agronomy and Soil Science》2017,63(12):1763-1775

This study is focused on the relative importance of bioclimatic variables and soil moisture index (SMI) for predicting forage production in Central Iran rangelands. We used a dataset of 93 forage production collected from 16 rangeland sites during 8 years in Isfahan province. Climatic and topographic variables were obtained using a digital elevation model and geographical information system. SMI is derived using a triangle space concept between the land surface temperature and normalized difference vegetation index from MODIS satellite data over Isfahan province. Using Nonparametric Multiplicative Regression model, we produced two distinct models to predict forage production, the first model with climatic and topographic variables (reduced model) and the second model with climatic, topographic and SMI variables (complete model). The inclusion of SMI significantly improved the quality of forage production predictions within the study area. The forage production is predicted to vary from 41.4 to 474 kg ha^?1 in first model and 20.5 to 485 kg ha^?1 in second model. We anticipated that using SMI would improve standing crop estimates relative to using bioclimatic variables. The cross-validated R² (×R²) increased from 57% to 63% when SMI is added to NPMR model.  相似文献   

Impacts of Woody Biochar Particle Size on Porosity and Hydraulic Conductivity of Biochar-Soil Mixtures: An Incubation Study     

Leila Esmaeelnejad  Mehdi Shorafa  Manouchehr Gorji  Seiyed mossa Hosseini 《Communications in Soil Science and Plant Analysis》2017,48(14):1710-1718

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A combination of gum arabic and chitosan can control anthracnose caused by Colletotrichum musae and enhance the shelf-life of banana fruit     

Mehdi Maqbool  PeteR Alderson 《The Journal of Horticultural Science and Biotechnology》2013,88(5):432-436

Summary

Anthracnose, caused by Colletotrichum musae, is the major disease affecting the quality of banana fruit during storage. To control banana anthracnose, the combined effects of coatings with gum arabic [GA; at 5, 10, 15 or 20% (w/v)] and 0.75% (w/v) chitosan (CH) were investigated and compared to untreated controls. In vitro results showed significant (P < 0.05) inhibition of mycelial growth and conidial germination of C. musae in all combined treatments compared to the untreated controls after 7 d of incubation at room temperature (25°C). However, potato dextrose agar (PDA) plates amended with 10% (w/v) GA gave the most promising results among all test treatments in suppressing mycelial growth (86%) and inhibiting conidial germination (80%), while no effective inhibition of conidial germination was observed in the controls. In vivo results confirmed that 10% (w/v) GA was the optimum concentration to control fruit decay (70%), while showing efficacy on the reduction of growth of C. musae on artificially inoculated banana fruit. The combined coatings of GA + CH also significantly delayed ripening in terms of weight loss, fruit firmness, soluble solids content, and titratable acidity. These results support the possibility of using 10% (w/v) GA combined with 0.75% (w/v) CH as an alternative strategy to control post-harvest anthracnose disease in banana fruit.  相似文献   

Polycyclic Aromatic Hydrocarbons in Seawater, Sediment, and Rock Oyster Saccostrea cucullata from the Northern Part of the Persian Gulf (Bushehr Province)     

Roozbeh Mirza  Mehdi Mohammadi  Ali Dadolahi Sohrab  Alireza Safahieh  Ahmad Savari  Pavaneh Hajeb 《Water, air, and soil pollution》2012,223(1):189-198

The concentration of polycyclic aromatic hydrocarbons (PAHs) was determined in seawater, sediment, and Rock oyster Saccostrea cucullata collected from four sampling sites in the inter-tidal areas of Bushehr province. The total concentrations of 14 PAHs varied from 1.5 to 3.6 ng/L in seawater, 41.7 to 227.5 ng/g dry weight in surface sediment, and 126 to 226.1 ng/g dry weight in oyster tissue. In comparing PAH concentrations among the three matrices in Bushehr province, data showed that the pattern of individual PAHs in seawater, oyster, and sediment were different. The oysters tended to accumulate the lower molecular weight and the more water-soluble PAHs. Sediment samples were distinguished from the sea water and oyster samples by the presence of high molecular weight PAHs, especially six-ring PAHs. Three- and four-ring PAHs were the most abundant compounds among the 14 PAHs investigated in surface seawater, sediment, and oyster samples. As expected, differences in octanol/water partition coefficient among individual PAHs and the greater persistence of the higher molecular weight PAHs contributed to the accumulation patterns in oyster and sediment. The results of the study suggested that the main sources of PAHs in the seawater and sediment in the region were mixed pyrolitic and petrogenic inputs.  相似文献   

The Healing Potential of Plantago lanceolata Ointment on Collagenase-Induced Tendinitis in Burros (Equus asinus)     

Mohammad Mehdi Oloumi  Dariush Vosough  Amin Derakhshanfar  Mohammad Hadi Nematollahi 《Journal of Equine Veterinary Science》2011,31(8):470-474

This study was carried out to evaluate the healing potential of water-soluble extract of Plantago lanceolata as a topical ointment on experimental tendinitis in burros. Tendinitis was induced by injection of 2,500 U of bacterial collagenase in the superficial digital flexor tendon of both forelimbs of six male burros. Tendinitis was confirmed ultrasonographically. 48 hours after collagenase injection, two grams of 10% P lanceolata ointment was applied on one limb (treatment group) and the same amount of eucerin (the vehicle of the ointment) on the other limb (control group), every other day for 6 weeks. Ultrasonographic examination of the tendon was performed weekly during the study and mean cross-sectional area of the superficial digital flexor tendon was measured, which was found to increase significantly after injection of collagenase in both groups. From the third week, it decreased significantly in the treatment group in comparison with the control group. At the end of the study, the animals were euthanized for histopathological study of the tendons. In control group, degenerative changes in tendon could be seen, whereas significant degree of healing was observed in the treatment group. It can thus be concluded that the P lanceolata ointment was effective in promoting the healing process of the tendon.  相似文献   

Early Pregnancy Detection of Iraqi Riverine Buffalo (Bubalus bubalis) Using the BioPRYN Enzyme‐Linked Immunosorbent Assay for PSPB and the Progesterone Assay     

TA Abdulkareem  SAM Al‐Sharifi  MA Ishak  SM Eidan  MA Alnimr  CW Passavant  JR Branen  RG Sasser 《Reproduction in domestic animals》2011,46(3):455-462

This study was undertaken to detect pregnancy in Iraqi riverine buffalo (Bubalus bubalis) using three different methods (rectal palpation, plasma progesterone concentration and detection of the presence of pregnancy‐specific protein B (PSPB) with the BioPRYN^® enzyme‐linked immunosorbent assay (ELISA) test. The aim of the study was to identify the most sensitive, early and accurate method for detecting pregnancy. Twenty‐two female riverine buffalo that were 6.0 ± 0.93 years old were used. Four blood samples per buffalo were taken via jugular venipuncture at days 22–24, 32–34, 42–44 and 58–61 post‐mating (PM) to measure the progesterone concentration (ng/ml) and to detect the presence of plasma PSPB. The rectal palpation method was employed to evaluate all buffalo on days 42–44 and 58–61 PM. The BioPRYN^® test differed (p < 0.01) from the other tests with earlier accuracy for detecting pregnant and non‐pregnant buffalo. Eighty‐eight percent of pregnant and 76.9% of non‐pregnant buffalo were distinguished early (days 22–24 PM) using BioPRYN^® and plasma PSPB‐ELISA level (2.09 ± 0.12 ng/ml) in relation to 66.7% and 53.9% detected using the progesterone assay at similar days (4.30 ± 0.40 ng/ml). In conclusion, these results described, for the first time, the early and accurate pregnancy detection of water riverine buffalo using BioPRYN^® technology and provided the plasma levels of PSPB using an ELISA test. These findings will improve the reproductive and productive efficiency of Iraqi riverine buffalo by adapting the recent management and reproductive strategies in Iraq and in the world.  相似文献