Expression of estrogen receptor alpha and beta in the uterus and vagina of immature rats treated with 17-ethinyl estradiol   总被引：3，自引：0，他引：3  

Kang JS  Lee BJ  Ahn B  Kim DJ  Nam SY  Yun YW  Nam KT  Choi M  Kim HS  Jang DD  Lee YS  Yang KH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(12):1293-1297

The action of estrogen on target organs has been actively studied with the discovery of estrogen receptor (ER) beta. This study was carried out to examine the expression of ERalpha and ERbeta in the uterus and the vagina of immature Sprague-Dawley rats treated with 17-ethinyl estradiol (EE). Twenty days old rats were subcutaneously treated with EE at the doses of 0 (vehicle control), 0.03, 0.3, 1.0, 3.0, and 10.0 microg/kg/day for three consecutive days. The treatment of EE at the doses of 0.3, 1.0, 3.0 and 10.0 microg/kg/day significantly increased the weights of the uterus and vagina of rats (p<0.01) and retained fluid in the uterus of rats. At the high doses of 3.0 and 10.0 microg/kg/day, the treatment of EE caused an increase in the uterine height, hypertrophy, and a decrease in the expression of ERalpha and ERbeta in the uterine luminal and glandular epithelium. The treatment of EE at the doses of 3.0 and 10.0 microg/kg/day also caused cornification and a decrease in the expression of ERalpha and ERbeta in the vaginal epithelium. These results suggest that the EE treatment decrease the expression of ERalpha and ERbeta in the uterus and vagina of immature rats and that may be associated with the morphological changes such as increase in the uterine height, hypertrophy of the uterine epithelium, and cornification of the vagina.  相似文献   

An oligonucleotide probe for the detection of Erwinia herbicola and Erwinia ananas     

Yoshihiro Nakanishi  Appolinaire Adandonon  Ikuko Okabe  Yuko T. Hoshino  Naoyuki Matsumoto 《Journal of General Plant Pathology》2006,72(5):328-333

An oligonucleotide probe targeting the rRNA of Erwinia herbicola and Erwinia ananas was designed to detect their cells using fluorescence in situ hybridization. The Cy3-labeled probe hybridized strongly with these species but very weakly with nontarget species such as Erwinia mallotivora, Erwinia nigrifluens, Erwinia cypripedii, Erwinia chrysanthemi, Erwinia carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Escherichia coli. This technique visualized E. herbicola cells after inoculation of kumquat fruits. The probe is promising as a tool for studying population dynamics of E. herbicola and E. ananas.  相似文献   

Omental herniation through the esophageal hiatus in a cat     

Mitsuoka K  Tanaka R  Nagashima Y  Hoshi K  Matsumoto H  Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1157-1159

A four-year-old male cat was presented with regurgitation. Thoracic radiography and contrast radiogram showed a large oval mass and elevated esophagus. Exploratory thoracotomy showed omental herniation into the posterior mediastium through the esophageal hiatus. Because the mass of the omental herniation was so large, celiotomy through a paracostal incision was combined in order to return the omentum to its normal position. The diameter of the esophageal hiatus was approximately 1 cm but no fibrous adhesion was observed around the hiatus. Continuous 1-0 surgical sutures on the hiatus reduced the diameter of the hiatus. The cat made a successful postoperative recovery without complications.  相似文献   

Pythium rot of Chinese cabbage (Brassica rapa L. subsp. pekinensis) caused by Pythium aphanidermatum     

Motoaki Tojo  Tatsurou Shigematsu  Hisataka Morita  Yingjie Li  Tadashi Matsumoto  Satoshi T. Ohki 《Journal of General Plant Pathology》2005,71(5):384-386

Severe rot was found at the base of leaves and stems of Chinese cabbage (Brassica rapa L. subsp. pekinensis) in Ibaraki Prefecture every year in early September from 2002 through 2004. The causal fungus was identified as Pythium aphanidermatum (Edson) Fitzpatrick. This is the first report of P. aphanidermatum on Chinese cabbage. A similar disease of Chinese cabbage caused by P. ultimum Trow var. ultimum is known as Pythium rot. We propose adding P. aphanidermatum as a new pathogen of this disease.  相似文献   

Secretory antibody responses in cattle infected with foot-and-mouth disease virus.     

M Matsumoto  P D McKercher  K E Nusbaum 《American journal of veterinary research》1978,39(7):1081-1087

Antibody responses in serum, saliva, nasal secretions, or esophageal-pharyngeal fluid of foot-and-mouth disease virus-infected steers were examined by single radial immunodiffusion and mouse-neutralization tests. In steers infected with type O foot-and-mouth disease virus, high serum antibody titers were detected within 10 days after infection. Antibody was first detected in saliva at 30 days and gradually increased to a plateau at about 90 days. Small amounts of antibody continued to be secreted in saliva and in nasal secretions for at least 6 months. Antibody was not detected in esophageal-pharyngeal fluid. The major antibody activity in secretions was due to secretory immunoglobulin A as revealed by radioimmunoelectrophoresis.  相似文献   

d -Alanine-d -alanine ligase gene ( ddl ) of Erwinia chrysanthemi strain EC16 II: analysis of pectate lyase regulation using ddl ^? mutant     

Pongphen Jitareerat  Hiroyuki Matsumoto  Masahiro Umehara  Shinji Tsuyumu 《Journal of General Plant Pathology》2003,69(1):49-54

 Erwinia chrysanthemi (Ech) triggers soft rot disease mainly by secreting pectate lyase (Pel), which is regulated in a complex manner by many regulatory genes. In a previous study, we used a gene dosage method to show that the ddl gene, which encodes d-alanine-D-alanine ligase, reduced Pel production and tissue maceration by Ech strain EC16n. In this study, the ddl ⁻ marker-exchanged mutant was shown to overcome the long growth lag caused by various salts in the growth medium and to increase Pel production over that by EC16n, especially in a medium containing magnesium salts. Thus, ddl seems to regulate Pel production in a negative manner. Because the profiles of a gel shift assay using the pelE promoter region as the target DNA with crude extracts of EC16n and ddl ⁻ mutant were distinguishable, Ddl is thought to affect the binding of other regulatory proteins. Expression of the ddl gene was induced in the medium containing a low-molecular-weight fraction of potato extract, but it was reduced in that containing both polygalacturonic acid (PGA) and the fraction. The repression of ddl expression by PGA should contribute in part to the in planta hyperinduction of Pel. Received: May 15, 2002 / Accepted: June 20, 2002  相似文献   

Production efficiency and telomere length of the cloned pigs following serial somatic cell nuclear transfer   总被引：2，自引：0，他引：2  

Kurome M  Hisatomi H  Matsumoto S  Tomii R  Ueno S  Hiruma K  Saito H  Nakamura K  Okumura K  Matsumoto M  Kaji Y  Endo F  Nagashima H 《The Journal of reproduction and development》2008,54(4):254-258

The aim of the present study was to examine the production efficiency of cloned pigs by serial somatic cell nuclear transfer (SCNT) and to ascertain any changes in the telomere lengths of multiple generations of pigs. Using fetal fibroblasts as the starting nuclear donor cells, porcine salivary gland progenitor cells were collected from the resultant first-generation cloned pigs to successively produce second- and third-generation clones, with no significant differences in production efficiency, which ranged from 1.4% (2/140) to 3.3% (13/391) among the 3 generations. The average telomere lengths (terminal restriction fragment values) for the first, second and third generation clones were 16.3, 18.1 and 20.5 kb, respectively, and were comparable to those in age-matched controls. These findings suggest that third-generation cloned pigs can be produced by serial somatic cell cloning without compromising production efficiency and that the telomere lengths of cloned pigs from the first to third generations are normal.  相似文献   

Reaction selectivity of active oxygen species produced by oxygen-alkali oxidation of a phenolic compound   总被引：1，自引：1，他引：0  

Tomoya Yokoyama  Iwao Maekawa  Yuji Matsumoto  Gyosuke Meshitsuka 《Journal of Wood Science》1998,44(5):421-422

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Evaluation of the extent of the oxidation reaction during chlorine bleaching of pulp     

Keiichi Koda  Hiroyuki Shintani  Yuji Matsumoto  Gyosuke Meshitsuka 《Journal of Wood Science》1999,45(2):149-153

A modified method was developed to evaluate how much chlorine is consumed by the oxidation reaction during the chlorine bleaching process. This evaluation is, in principle, based on the sum of chloride produced during the chlorination stage (C-stage) and produced during alkali treatment of both the C-stage effluent and the chlorinated pulp. Results obtained by this method proved that about 50%–75% of chlorine was consumed by the oxidation reaction during chlorine bleaching, depending on the reaction condition of chlorination. Even under a reaction condition that is not favorable to an oxidation reaction (low pH), approximately three electrons were abstracted from one lignin structural unit by chlorine bleaching. This result provides additional evidence for our recent observation that lignin is extensively oxidized during chlorine bleaching even when pure chlorine without any chlorine dioxide substitution was used.Part of this paper was presented at the 40th Lignin Symposium. Tsukuba, Japan, October 12, 1995  相似文献   

Breeding and characterization of the world’s first practical rice variety with resistance to brown spot (Bipolaris oryzae) bred using marker-assisted selection     

Kengo Matsumoto  Yuya Ota  Tomohiro Yamakawa  Teppei Ohno  Satomi Seta  Yuto Honda  Ritsuko Mizobuchi  Hiroyuki Sato 《Breeding Science》2021,71(4):474

Brown spot (BS) caused by Bipolaris oryzae is a serious disease of rice and decreases grain yield. Breeding for BS resistance is an economical solution but has not hitherto been achieved. To develop a practical BS-resistant variety, we introduced a chromosomal segment including a quantitative trait locus (QTL) for BS resistance, qBSfR11, derived from the BS-resistant local resource ‘Tadukan’, into the genetic background of the high-yielding but susceptible ‘Mienoyume’. Resistance is controlled by a single recessive gene in a 1.3-Mbp region. We named this gene bsr1 (brown spot resistance 1). The near-isogenic line bsr1-NIL had a greater yield with larger grain width than Mienoyume but similar other agronomic traits in fields where BS was mild; it had a significantly lower BS disease score and a 28.8% higher yield in fields where BS was more severe, and it showed resistance to multiple isolates of BS fungus. It showed stable resistance to BS and had excellent agricultural traits in the presence of BS. We developed the bsr1-NIL with resistance to BS and applied it for variety registration to Ministry of Agriculture, Forestry and Fisheries in Japan as ‘Mienoyume BSL’. This is the first report for the BS resistant rice variety bred using marker-assisted selection.  相似文献