Genetic diversity and pathogenic characteristics of Phomopsis asparagi obtained from Asparagus officinalis and A. kiusianus in Japan     

Thao  Le Dinh  Matsumoto  Masaru 《植物病害和植物保护杂志》2020,127(6):843-853

Journal of Plant Diseases and Protection - Phomopsis asparagi has been reported as a serious disease, causing stem blight on asparagus cultivars (Asparagus officinalis), and this fungus was also...  相似文献   

QTL analysis for brown spot resistance in American rice cultivar ‘Dawn’     

Yuya Ota  Kengo Matsumoto  Yukinori Nakayama  Tomohiro Yamakawa  Teppei Ohno  Ritsuko Mizobuchi  Hiroyuki Sato 《Breeding Science》2021,71(4):491

Rice brown spot (BS), caused by Bipolaris oryzae, causes yield loss and deterioration of grain quality. Using single-nucleotide polymorphism (SNP) markers, we conducted quantitative trait locus (QTL) analysis of BS resistance in backcross inbred lines (BILs) from a cross between an American rice cultivar, ‘Dawn’ (resistant), and ‘Koshihikari’ (susceptible). Four QTLs for BS resistance were detected in a three-year field evaluation, and ‘Dawn’ contributed the resistance alleles at all QTLs. The QTL with the greatest effect, qBSR6-kd, explained 15.1% to 20.3% of the total phenotypic variation. Although disease score and days to heading (DTH) were negatively correlated in all three years, qBSR6-kd was located near a QTL for DTH at which the ‘Dawn’ allele promoted heading. Another BS resistance QTL (qBSR3.1-kd) was unlinked to the QTLs for DTH. Therefore, these two QTLs are likely to be useful for breeding BS-resistant varieties without delaying heading. The other two BS resistance QTLs (qBSR3.2-kd and qBSR7-kd) were located near DTH QTLs at which the ‘Dawn’ alleles delayed heading. The QTLs reported here will be good candidates for developing BS-resistant cultivars.  相似文献   

Breeding and characterization of the world’s first practical rice variety with resistance to brown spot (Bipolaris oryzae) bred using marker-assisted selection     

Kengo Matsumoto  Yuya Ota  Tomohiro Yamakawa  Teppei Ohno  Satomi Seta  Yuto Honda  Ritsuko Mizobuchi  Hiroyuki Sato 《Breeding Science》2021,71(4):474

Brown spot (BS) caused by Bipolaris oryzae is a serious disease of rice and decreases grain yield. Breeding for BS resistance is an economical solution but has not hitherto been achieved. To develop a practical BS-resistant variety, we introduced a chromosomal segment including a quantitative trait locus (QTL) for BS resistance, qBSfR11, derived from the BS-resistant local resource ‘Tadukan’, into the genetic background of the high-yielding but susceptible ‘Mienoyume’. Resistance is controlled by a single recessive gene in a 1.3-Mbp region. We named this gene bsr1 (brown spot resistance 1). The near-isogenic line bsr1-NIL had a greater yield with larger grain width than Mienoyume but similar other agronomic traits in fields where BS was mild; it had a significantly lower BS disease score and a 28.8% higher yield in fields where BS was more severe, and it showed resistance to multiple isolates of BS fungus. It showed stable resistance to BS and had excellent agricultural traits in the presence of BS. We developed the bsr1-NIL with resistance to BS and applied it for variety registration to Ministry of Agriculture, Forestry and Fisheries in Japan as ‘Mienoyume BSL’. This is the first report for the BS resistant rice variety bred using marker-assisted selection.  相似文献   

Effect of Gaseous Acetic Acid on Dough Rheological and Breadmaking Properties     

Masaharu Seguchi  Machiko Hayashi  Hiroshi Matsumoto 《Cereal Chemistry》1997,74(2):129-134

Breads baked from wheat flours (protein contents 14.1–16.5% at 14.0% mb) that were pretreated with 2–3 mL of gaseous acetic acid per kg of wheat flour, showed maximum bread height and specific volume (cm³/g). Flour-water suspension and the crumb pH values were gradually decreased with increased amounts of acetic acid. Gas generation and dough expansion tests with bread dough showed that the addition of the same amount of acetic acid, which achieved maximum specific volume, also showed the highest rate of gas generation and dough expansion. However, increasing acetic acid decreased these values. Scanning electron microscope (Cryo-SEM) observation showed that the bread dough made from the same acetic acid-treated flour indicated continuum and no cracks in the dough matrix. Evaluation of mixograms showed the decrease of mixing stability with increased acetic acid levels. Viscosity and water binding capacity of flour-water suspensions were sharply increased by the addition of acetic acid at pH 5.0–3.5.  相似文献   

Difference Between Tetrodotoxin and Saxitoxins in Accumulation in Puffer Fish Takifugu rubripes Liver Tissue Slices     

T. Matsumoto  Y. Nagashima  K. Takayama  K. Shimakura  K. Shiomi 《Fish physiology and biochemistry》2006,31(1):95-100

In vitro accumulation of tetrodotoxin (TTX) and paralytic shellfish toxin (PST) in tiger puffer fish Takifugu rubripes was investigated using liver tissue slices. When T. rubripes liver slices were incubated with Leibovitz’s L-15 medium containing 0.13 mM TTX at 20 °C in air with saturated humidity, they accumulated 21.5 ± 7.3 μg TTX g⁻¹ liver after the incubation for 12 h and increased to 55.3 ± 8.2 μg TTX g⁻¹ liver at 48 h. In the incubation of T. rubripes liver slices with 0.13 mM PST-containing medium, PST was detected 6.3 ± 0.9 μg g⁻¹ liver at 12 h and reached a plateau thereafter. These results reveal the difference between TTX and PST in accumulation in T. rubripes liver tissue slices. To examine the variation in PST accumulation among fish species, the liver tissue slices from tiger puffer fish T. rubripes, parrot-bass Oplegnathus fasciatus and green ling Hexagrammos otakii were incubated at a concentration of 0.027 mM PST. The toxin contents of 3.0 μg g⁻¹ liver were observed at 8 h regardless of fish species but were not increased subsequently, showing no variety among these three species as to accumulation patterns of PST. It is noted that the tiger puffer fish T. rubripes liver specifically accumulate TTX in preference to PST.  相似文献   

Human focal nodular hyperplasia-like lesion in the liver of a cat     

K Ochiai  M Takechi  T Matsumoto  C Itakura 《The Japanese journal of veterinary research》1990,38(3-4):117-125

A young cat clinically displaying marked ascites had multiple nodule hyperplasia in the liver. One of the nodules sampled at exploratory laparotomy was studied histopathologically. The nodule consisted mainly of hyperplastic hepatocytes with a central stellate scarlike connective tissue. The connective tissue had thick-walled blood vessels and radiating bands of fibrous connective tissue extending peripherally, dividing the nodule into pseudolobules. The lesions resembled focal nodular hyperplasia (FNH) in man. Serial section revealed a single large artery with the splitting of the internal elastic lamina running into the central connective tissue and branching to connect with the main vessels. The arterial branchings formed a spider-like structure. From these findings, it was suggested that the nodule had developed from a hepatic response to pre-existing vascular anomalies as had been reported in human FNH.  相似文献   

An improved method for the purification of blood cells of turkeys     

J G Strain  M Matsumoto 《Avian diseases》1991,35(1):221-223

Methods were developed to purify monocytes, heterophils, or erythrocytes from the whole blood of turkeys. After the bulk of thrombocytes was removed by centrifugation, blood was fractionated over a Ficoll-Hypaque discontinuous gradient. Five fractions were harvested separately, and two were further purified by attachment to a plastic surface and/or by lysis of erythrocytes. The monocyte fraction contained 41% +/- 1% monocytes, and the heterophil fraction contained 96% +/- 1% heterophils. The erythrocyte fraction showed a purity of 99.4% +/- 0.3%. These methods would be useful for various in vitro studies that require purified populations of blood cells.  相似文献   

Nonspecific reactions to Mycoplasma antigens caused in turkeys sera by Erysipelothrix insidiosa bacterin.     

C H Bigland  J J Matsumoto 《Avian diseases》1975,19(3):617-621

Nonspecific reactions to Mycoplasma meleagridis (MM) and M. gallisepticum antigens were found in the sera of turkeys vaccinated with Erysipelothrix insidiosa (EI) bacterin, but they could be removed by adsorption with EI bacterin. True reactions to MM could not be so removed.  相似文献   

Formation of glutamine and activities of glutamine synthetase in goats.     

Y Ide  H Matsumoto 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1977,39(2):159-164

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Experimental study of materials for patch graft on right ventricular outflow tract under extracorporeal circulation in dogs--comparison between Denacol EX-313-treated bovine jugular vein graft and expanded polytetrafluoroethylene (EPTFE) graft     

Matsumoto H  Sugiyama S  Shibazaki A  Tanaka R  Takashima K  Noishiki Y  Yamane Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(9):961-965

A comparison between a bovine jugular vein treated with a hydrophilic polyepoxy compound cross-linker (Denacol), and expanded polytetrafluoroethylene (EPTFE), an artificial material, as a patch graft for the reconstruction of the right ventricular outflow tract under extracorporeal circulation in dogs, as if they had pulmonic stenosis, was made. Hemodynamic and histological examinations were conducted two weeks after the transplantation. Hemodynamic problems were not observed in either the Denacol or EPTFE groups. Macroscopically, organization of new tissue on the graft surface was more marked in the EPTFE group than in the Denacol group, and newly-formed tissue was seen surrounding the border of the graft and burying it in the EPTFE group. In the Denacol group, microscopic findings revealed the presence of inflammatory cells and fibroblasts, and an invasion of the graft by collagen fibers and elastic fibers. In the EPTFE group, there was minimal cellular infiltration of the graft and a thick layer consisting of collagen fibers and fibroblasts was observed around the graft. These results indicated that two weeks after transplantation the graft was better assimilated and organized with blood vasculature in the patch graft in the Denacol group than in the EPTFE group.  相似文献