Concepts and Direction of Induced Systemic Resistance in Plants and its Application   总被引：11，自引：0，他引：11  

Joseph Kuć 《European journal of plant pathology / European Foundation for Plant Pathology》2001,107(1):7-12

Resistance to plant disease is often specific and metabolites and receptors contributing to this specificity may have specific structures. However, simple, structurally-unrelated compounds induce systemic resistance in unrelated plants to diverse pathogens including fungi, bacteria and viruses. Both resistance and induced systemic resistance (ISR) are associated with the rapid accumulation of the same structurally unrelated putative defense compounds that have diverse functions. It has been suggested that cultivar (race)-specific resistance is initiated by the specific interaction of a pathogen product (or pathogen induced product) and a plant receptor. However, restricted infection by pathogens can result in ISR and many different compounds can cause ISR. It is thus evident that there are both specific and non-specific routes to the master switch for ISR and there may be more than one master switch. Are reactive oxygen species and free radicals regulating the master switch(es) via both routes? It is also evident there are many switches, other than the master switch. Adding to the complexity of resistance and ISR are the observations that different compounds and pathways may mediate different biochemical resistances. Activation of one of the pathways may antagonize or enhance the activation or effectiveness of another. The review will address these complexities and questions and propose directions of research which require high priority. Factors which encourage and suppress the application of ISR in agriculture will also be addressed.  相似文献   

Erythroid progenitor cells from pig bone marrow and peripheral blood     

Kovacević M  Bozić T  Ivanović Z 《Veterinary journal (London, England : 1997)》1999,158(3):143-203

With the intention of using the pig as a large animal model in haematopoietic research, a clonal assay in methylcellulose was developed and the optimal conditions for raising erythroid progenitors from adult pig bone marrow (BM) and peripheral blood (PB) have been established. Progenitor cells were stimulated to proliferate and differentiate in vitro by growth factors containing leucocyte condition medium (LCM), and with recombinant human erythropoietin (rhEpo). The number of PB BFU-E (burst forming units - erythroid) directly depended on the concentration of LCM, but BM BFU-E were not dependent on LCM. Both CFU-E (colony forming units - erythroid) and BFU-E were rhEpo dependent. Despite relatively high but expected individual variations, the mean number of colonies, as well as the functional characteristics of progenitor cells investigated, were similar to those of miniature pigs and some other mammals.  相似文献   

Effect of different daily doses of gizzerosine on the serum 1,25-dihydroxycholecalciferol concentration in laying hens     

Mrljak V  Grabarević Z  Dzaja P  Barić Rafaj R  Perić J  Artuković B  Bidin Z  Kis S  Stavljenić Rukavina A  Serman V 《British poultry science》1999,40(4):523-528

Five groups of laying hens were treated with different gizzerosine doses (0, 2.5; 5.0; 7.5; 10.0 mg/kg/body weight of gizzerosine) daily over a 21-day period to determine the serum concentrations of 1.25-dihydroxycholecalciferol (1,25(OH)2D), total calcium, inorganic phosphorus and magnesium. Blood samples were taken on days 7, 14, and 21 of the experiment. The concentration of 1,25(OH)2D remained unchanged after day 7 in the gizzerosine-treated birds compared to the control group. After 14 days, it was significantly lower in the birds receiving. gizzerosine, compared with the control group. On day 21, 1,25(OH2)D concentrations were also significantly decreased in all 4 gizzerosine-treated groups compared with the control hens. The serum total calcium, inorganic phosphorus and total magnesium concentrations varied significantly, but irregularly, during the period of the study.  相似文献   

Comparison of antibody values in sera of pigs vaccinated with a subunit or an attenuated vaccine against classical swine fever     

Terzić S  Jemersić L  Lojkić M  Madić J  Grom J  Toplak I  Sver L  Valpotić I 《Veterinary research communications》2003,27(4):329-339

Ten pigs, aged 85 days, were vaccinated with a subunit vaccine containing 32 g of classical swine fever virus glycoprotein E2 (gp E2) (group 1), and a further 10 pigs were vaccinated with a C strain vaccine (10^4±0.15 TCID₅₀/ml), produced by amplification in minipig kidney (MPK) cell culture (group 2). Nine non-vaccinated pigs served as a control group (group 3). Serum samples were collected before (day 0) and at 4, 10, 21 and 28 days after vaccination and were analysed by two commercially available enzyme immunoassays and by a neutralizing peroxidase-linked assay (NPLA). At the same times, peripheral blood was taken for determining the total leukocyte count and the body temperature was taken daily. Antibodies were not detected in serum samples collected before vaccination (day 0), and no side-effects that could be connected with vaccination were observed during the trial. Ten days after vaccination 6/10 pigs vaccinated with the subunit vaccine were seropositive. On days 21 and 28, the ratios of serologically positive to vaccinated pigs were 9/10 and 10/10, respectively. Four of the ten pigs that were vaccinated with the C strain vaccine were positive on day 21 and 9/10 on day 28. However, the results of the NPLA showed that only 4/10 pigs had an antibody titre >1:32 at the end of the trial in both the vaccinated groups, even though the subunit vaccine initiated an earlier and higher level of neutralizing antibodies than the vaccine produced from the C strain. Challenge was performed 28 days after vaccination on four randomly selected pigs from both vaccinated groups. The pigs survived the challenge without showing any clinical signs of classical swine fever (CSF), while two nonvaccinated control pigs died on the 10th and 12th days after infection.  相似文献   

In vitro antimicrobial susceptibility of 183 Pseudomonas aeruginosa strains isolated from dogs to selected antipseudomonal agents     

Seol B  Naglić T  Madić J  Bedeković M 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2002,49(4):188-192

During the years from 1993 to 2000, 183 strains of Pseudomonas aeruginosa were isolated from different pathological specimens originating from dogs. Antimicrobial susceptibility patterns against 10 antipseudomonal agents were obtained on 183 P. aeruginosa strains. In vitro antimicrobial susceptibility testing was performed using the disk diffusion method (Kirby-Bauer). Antimicrobial susceptibility profiles showed that among beta-lactam antibiotics, imipenem was the most active compound. Out of the 183 strains tested, 96.7% were sensitive to imipenem. Cefoperazone showed good in vitro activity against 86.9% of the tested strains. Against ceftazidime, 77.0% of strains showed sensitivity. An old penicillin, carbenicillin, gave only 71.6% sensitive strains. Sensitivity to amikacin was 87.4% and it was 83.1% to gentamicin. Pipimedic acid, a first-generation quinolone, was the least active compound of all those tested, 47.0% were resistant. The in vitro sensitivity against enrofloxacin showed that 71.0% strains were sensitive and 26.2% showed resistance. Almost all strains tested, 93.4%, were susceptible to ciprofloxacin and marbofloxacin. Besides imipenem, the quinolone antibiotics, marbofloxacin and ciprofloxacin were the most effective against P. aeruginosa strains isolated from dogs.  相似文献   

Occurrence of enteric redmouth disease in rainbow trout (Oncorhynchus mykiss) on farms in Croatia     

Oraić D  Zrncić S  Sostarić B  Bazulić D  Lipej Z 《Acta veterinaria Hungarica》2002,50(3):283-291

During the spring of 1996 and autumn of 1997 unusual mortality outbreaks among rainbow trout fry and yearlings occurred at two different trout farms, resulting in mortality of 20 and 10 per cent, respectively. Generally, the affected fish, swimming at the water surface, were reluctant to eat and were dark pigmented with visible haemorrhages around and within the oral cavity. Bacterial isolates from moribund fish from both cases were identified as Yersinia ruckeri by standard biochemical tests and API 20E. The isolated strains were found to be sensitive to tetracycline, chloramphenicol, co-trimoxazole, nalidixic acid, flumequine, enrofloxacin, carbenicillin and gentamicin. Microplate agglutination assay confirmed that both isolates belonged to serotype O1. The pathogenicity of the isolated bacteria was confirmed by challenge experiment. Titres of specific antibodies were determined in the sera of survivors. The titre was highest on the 21st day postchallenge and was detectable until the 81st day.  相似文献   

A survey of chickens for viable toxoplasms in Croatia     

Kuticić V  Wikerhauser T 《Acta veterinaria Hungarica》2000,48(2):183-185

Brain tissues of 716 slaughtered domestic chickens (524 broilers and 192 hens) were bioassayed for viable toxoplasms. Each tissue was homogenized and subcutaneously injected into 4 SPF mice. Six weeks later the mice were euthanatized and their brains microscopically examined for Toxoplasma gondii tissue cysts. Three (0.4%) out of a total of 716 birds were positive. All positive cases were hens. This is the first isolation of T. gondii from chickens in Croatia.  相似文献   

Entwicklung und Zuwachs einiger Nadelbaumkulturen in Mittel- und Ost-Serbien     

V. Stamenković  V. Miščević 《European Journal of Forest Research》1973,92(1):275-291

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Parasitoid complex (Hymenoptera, Braconidae, Aphidiinae) of Aphis craccivora Koch (Hemiptera: Aphidoidea) in Iran     

E. Rakhshani  A. A. Talebi  N. G. Kavallieratos  A. Rezwani  S. Manzari  Željko Tomanović 《Journal of pest science》2005,78(4):193-198

The parasitoids (Hymenoptera, Braconidae, Aphidiinae) of the cowpea aphid, Aphis craccivora Koch, were investigated throughout two far distant provinces, Tehran and Sistan-Baluchestan. Colonies of the cowpea aphid were collected from different host plants and reared under laboratory conditions until the parasitoids emerged. The aphids were parasitized by an expected range of parasitoids. The species were Aphidius colemani Viereck, Lysiphlebus fabarum (Marshall), Lysiphlebus confusus Tremblay and Eady, Lysiphlebus testaceipes (Cresson), Binodoxys acalephae (Marshall), Binodoxys angelicae (Haliday), Praon volucre (Haliday) and Ephedrus persicae Froggatt. L. testaceipes is reported here for the first time in Iran. The most common species was L. fabarum followed by B. acalephae and L. confusus. A key is provided for identification and host plant associations are considered.  相似文献   

Bemerkungen über die Populationsbewegungen der Mittelmeerfruchtfliege (Ceratitis capitata Vied.) an der jugoslawischen Adriaküste     

Željko Kovačević 《Journal of pest science》1965,38(10):151-153

Zusammenfassung Untersuchungen über den Massenwechsel und die Biologie der MittelmeerfruchtfliegeCeratitis capitata Vied. während der letzen 5 Jahre führten zu folgenden Ergebnissen:1. Die Mittelmeerfruchtfliege, die bis 1947 in ganz Jugoslawien unbekannt war, kommt seit mindestens zehn Jahren auch an der Adriaküste vor.2. Erst im Jahre 1958 erwies sich die Fruchtfliege als Schädling verschiedener Obstarten.3. Wie aus Beobachtungen in Obstanlagen an der Adriaküste hervorgeht, befällt die Fliege in erster Linie Pfirsich, ferner auch Birne, Kaki, Feige, Apfel, Pflaume, Orange und grünen Paprika. Im Laboratorium gelang die Zucht außerdem noch an Banane, nicht aber an Tomate und Trauben.4. ObwohlC. capitata ein polyphages Insekt ist, befällt sie an einigen Stellen, wo sie regelmäßig große Häufigkeit aufweist, nur Pfirsich (Split, Debeli Rti) bzw. nur Kaki (Ankaran). In manchen Obstgärten lebt sie aber an verschiedenen Früchten (Podstrana, Stroanac). Es gibt bei der Fruchtfliege also auf Obstarten spezialisierte Populationen und solche, die an verschiedenen Obstarten vorkommen.5. Unter den in unserem Küstenland herrschenden Klimabedingungen kann die Fruchtfliege in einem Jahr 4–5 Generationen entwickeln. Die Herbstgeneration verpuppt sich Ende Oktober/Anfang November in der Erde, die Imagines verlassen bis Januar die Puppen und warten auf das Reifen der ersten, frühen Pfirsichsorten. Die gleiche Überwinterungsweise wurde auch an der Olivenfliege beobachtet.6. Mit Angelica-Öl versehene Fanggläser brachten nur zum Teil befriedigende Ergebnisse, da dieser Lockstoff nur die Männchen anzieht, aber auch nur in der Zeit, in der die Fliegen überhaupt selten sind. Im Spätsommer und Herbst wirkt ein aus Kleie und Biammonphosphat bereiteter Köder auf beide Geschlechter stark anlockend.  相似文献