Quantitative real-time PCR assay for rapid detection of Pseudomonas amygdali pv. lachrymans in cucumber leaf rinse     

Gazdik  Filip  Penazova  Eliska  Cechova  Jana  Baranek  Miroslav  Eichmeier  Ales 《植物病害和植物保护杂志》2019,126(6):517-528

Journal of Plant Diseases and Protection - Pseudomonas amygdali pv. lachrymans (Pal) is a bacterium that causes angular leaf spot disease of cucumbers. There is no direct protection against this...  相似文献   

Forest management impacts on capercaillie (<Emphasis Type="Italic">Tetrao urogallus</Emphasis>) habitat distribution and connectivity in the Carpathians     

Martin Mikoláš  Martin Tejkal  Tobias Kuemmerle  Patrick Griffiths  Miroslav Svoboda  Tomáš Hlásny  Pedro J. Leitão  Robert C. Morrissey 《Landscape Ecology》2017,32(1):163-179

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Feeding activity of pikeperch (Sander lucioperca) under winter and spring photothermal conditions of pre‐spawning period     

Oleksandr Malinovskyi  Miroslav Blecha  Ji&#x;í K&#x;i&#x;&#x;an  Tom&#x; Policar 《Aquaculture Research》2020,51(2):852-857

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Effect of environmental variables on performance of Jerusalem artichoke (<Emphasis Type="Italic">Helianthus tuberosus</Emphasis> L.) cultivars in a long term trial: a statistical approach     

Miroslav Zorić  Sreten Terzić  Vladimir Sikora  Milka Brdar-Jokanović  Dimitar Vassilev 《Euphytica》2017,213(1):23

Tuber yield, together with tuber number and size are the basic agronomic and breeding traits in Jerusalem artichoke and can be significantly affected by environmental factors. We report the results of a long term trial on the performance of 20 Jerusalem artichoke cultivars. The random model for means with restricted maximum likelihood (REML) procedure was used to estimate the overall effects of the genotype, environment and genotype by environment interaction on traits. The partial least square regression (PLSR) model was used for modeling genotype by environment interaction variance components with a set of available correlated environmental variables. The REML variance component estimates model revealed that tuber number and yield are more dependent on GE interaction which allowed identification of best genotypes for specific environments. The PLSR model revealed that the most important climatic variables for optimal emergence, canopy development, high tuber number and yield are adequate soil and air temperatures in April. For larger tuber mass, precipitation variables and even distribution of rainfall were the most important factor, together with soil and air temperature in June when tuber growth is initiated. The knowledge obtained in this study is valuable for the identification and understanding of key environmental factors that contribute to the performance of Jerusalem artichoke.  相似文献   

Longitudinal Growth of Leg and Wing Bones of Chickens Reared in Cages and on the Floor     

D. Vitorovic  Z. Nikolic 《Anatomia, histologia, embryologia》1995,24(2):81-83

The histomorphometry of proximal growth plate and rate of longitudinal growth was estimated in tibia and humerus of chickens reared in the cages and on the floor, during 0, 2, 4, 6 and 8 weeks of age. The floor vs. the cage area was 3:1. All chicks were fed ad libitum standard broilers mixture: starter (0-4 weeks) with 0.9% Ca; 0.7% P and grower (4-8 weeks) with 0.8% Ca; 0.5% P. The rearing system showed no significant effect on macroscopy estimated tibia and humerus growth in length, just as in case of bone's proximal growth plate microscopy of floor reared chickens. However, our results suggested that wing bones are more affected by the housing system than that of leg bones. The proliferative zone, hypertrophic zone and total growth plate of the humerus in cage reared birds were significantly higher than in those reared on the floor.  相似文献   

The influence of early yield on the accumulation of major taste and health-related compounds in black and red currant cultivars (Ribes spp.)     

Milivojevic J  Slatnar A  Mikulic-Petkovsek M  Stampar F  Nikolic M  Veberic R 《Journal of agricultural and food chemistry》2012,60(10):2682-2691

The focus of our study was to investigate the effect of crop load on the accumulation and composition of primary metabolites (sugars and organic acids), selected groups of flavonoids (anthocyanins and flavonols), and total phenolics in two subsequent years in four black currant cultivars ('Titania', 'Triton', 'Tsema', and 'Cacanska crna') and three red currant cultivars ('Junifer', 'Rolan', and 'Stanza'). For the determination and quantification of compounds, high-performance liquid chromatography-photodiode array with a mass spectrometer was used. Significant differences among cultivars were detected in all analyzed compounds. Anthocyanins were the predominant phenolic group and were more abundant in black currant cultivars as compared to red ones. Similar amounts of sugars and organic acids were measured in both Ribes species; however, vitamin C was 3-fold higher in black currants. A larger crop load in the second year had a negative effect on the sugar content of berries and promoted a higher degree of acids, with the exception of vitamin C, which was higher in the year with a lower crop load. On the other hand, the content of anthocyanins and flavonols was higher in the year with a larger crop load, while there were no differences in total phenolic content.  相似文献   

Synthesis and fungicidal activity of N-2-(3-methoxy-4-propargyloxy) phenethyl amides. Part 3: stretched and heterocyclic mandelamide oomyceticides     

Lamberth C  Kempf HJ  Kriz M 《Pest management science》2007,63(1):57-62

Novel analogues of mandipropamid have been designed and prepared. The synthetic approach to these stretched and heterocyclic mandelamides is outlined. Biological data demonstrate their high efficacy against important plant diseases like tomato and potato late blight (Phytophthora infestans De Bary) and grape downy mildew (Plasmopara viticola Berliner & de Toni). Structure-activity relationship studies are discussed.  相似文献   

Molecular and phylogenetic analysis of the H5N1 avian influenza virus caused the first highly pathogenic avian influenza outbreak in poultry in the Czech Republic in 2007     

Nagy A  Vostinakova V  Pindova Z  Hornickova J  Cernikova L  Sedlak K  Mojzis M  Dirbakova Z  Machova J 《Veterinary microbiology》2009,133(3):257-263

On 19th July 2007 re-occurrence of the H5N1 highly pathogenic avian influenza (HPAI) virus was noticed in Europe. The index strain of this novel H5N1 lineage was identified in the Czech Republic where it caused historically the first HPAI outbreak in commercial poultry. In the present study we performed molecular and phylogenetic analysis of the index strain of the re-emerging H5N1 virus lineage along with the Czech and the Slovak H5N1 strains collected in 2006 and established the evolutionary relationships to additional viruses circulated in Europe in 2005-2006. Our analysis revealed that the Czech and the Slovak H5N1 viruses collected during 2006 were separated into two sub-clades 2.2.1 and 2.2.2, which predominated in Europe during 2005-2006. On the contrary the newly emerged H5N1 viruses belonged to a clearly distinguishable sub-clade 2.2.3. Within the sub-clade 2.2.3 the Czech H5N1 strains showed the closest relationships to the simultaneously circulated viruses from Germany, Romania and Russia (Krasnodar) in 2007 and were further clustered with the viruses from Afghanistan and Mongolia circulated in 2006. The origin of the Czech 2007 H5N1 HPAI strains was also discussed.  相似文献   

Distribution of Pseudomonas populations harboring phlD or hcnAB biocontrol genes is related to depth in vineyard soils     

Miroslav Svercel  Jérôme Hamelin  Brion Duffy  Geneviève Défago 《Soil biology & biochemistry》2010,42(3):466-472

The abundance and population structure of pseudomonads in soils collected from long-(1006 years) and short-(54 years) term grapevine monocultures in Switzerland were examined across five soil horizons within the 1.20-1.35 m range. Soil samples were baited with grapevine, and rhizosphere pseudomonads containing the biocontrol genes phlD (2,4-diacetylphloroglucinol synthesis) and/or hcnAB (hydrogen cyanide synthesis) were analyzed by MPN-PCR. The numbers of total, phlD⁺ and hcnAB⁺ pseudomonads decreased with depth by 1.5-2 log (short-term monoculture) and 3-3.5 log (long-term monoculture). In addition, the percentages of phlD⁺ (except in short-term monoculture) and hcnAB⁺ pseudomonads were also lower in deeper horizons. RFLP-profiling of phlD⁺ and hcnAB⁺ pseudomonads revealed three phlD and twelve hcnAB alleles overall, but the number of alleles for both decreased in relation to depth. The only phlD allele found in deeper horizons was also found in topsoil, whereas one hcnAB allele (k) found in deeper horizons in long-term monoculture was absent in the topsoil. This suggests that certain Pseudomonas ecotypes are adapted to specific depths. Four hcnAB alleles enabled discrimination between monocultures. We conclude that soil depth is a factor selecting phlD and hcnAB genotypes, and that the allelic diversity of the two biocontrol genes decreases with depth.  相似文献   

Grouping pattern of maize test locations and its impact on hybrid zoning     

Milisav Stojaković  Bojan Mitrović  Miroslav Zorić  Mile Ivanović  Dušan Stanisavljević  Aleksandra Nastasić  Dejan Dodig 《Euphytica》2015,204(2):419-431

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