Infection of HTLV-III/LAV in HTLV-I-carrying cells MT-2 and MT-4 and application in a plaque assay   总被引：120，自引：0，他引：120  

S Harada  Y Koyanagi  N Yamamoto 《Science (New York, N.Y.)》1985,229(4713):563-566

The human T-cell lines MT-2 and MT-4 carry the human T-cell leukemia virus type I (HTLV-I). When MT-2 and MT-4 were infected with HTLV-III, the probable etiologic agent of the acquired immune deficiency syndrome (AIDS), rapid cytopathogenic effects and cytotoxicity were observed that made it possible to titrate the biologically active virus in a plaque-forming assay. The cytopathogenic effects were preceded by the rapid induction and increase of HTLV-III antigens as revealed by immunofluorescence and immunoprecipitation. Activities of HTLV-III were neutralized by the human antibodies against the virus when immunofluorescence and plaque assays were used. Essentially the same results were obtained with the lymphadenopathy-associated virus (LAV1).  相似文献   

Oscillatory expression of the bHLH factor Hes1 regulated by a negative feedback loop   总被引：1，自引：0，他引：1  

Hirata H  Yoshiura S  Ohtsuka T  Bessho Y  Harada T  Yoshikawa K  Kageyama R 《Science (New York, N.Y.)》2002,298(5594):840-843

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Direct Observation of Vortex Dynamics in Superconducting Films with Regular Arrays of Defects     

K Harada  O Kamimura  H Kasai  T Matsuda  A Tonomura  VV Moshchalkov 《Science (New York, N.Y.)》1996,274(5290):1167-1170

The microscopic mechanism of the matching effect in a superconductor, which manifested itself as the production of peaks or cusps in the critical current at specific values of the applied magnetic field, was investigated with Lorentz microscopy to allow direct observation of the behavior of vortices in a niobium thin film having a regular array of artificial defects. Vortices were observed to form regular and consequently rigid lattices at the matching magnetic field, at its multiples, and at its fractions. The dynamic observation furthermore revealed that vortices were most difficult to move at the matching field, whereas excess vortices moved easily.  相似文献   

Oxygen isotopic compositions of asteroidal materials returned from Itokawa by the Hayabusa mission     

Yurimoto H  Abe K  Abe M  Ebihara M  Fujimura A  Hashiguchi M  Hashizume K  Ireland TR  Itoh S  Katayama J  Kato C  Kawaguchi J  Kawasaki N  Kitajima F  Kobayashi S  Meike T  Mukai T  Nagao K  Nakamura T  Naraoka H  Noguchi T  Okazaki R  Park C  Sakamoto N  Seto Y  Takei M  Tsuchiyama A  Uesugi M  Wakaki S  Yada T  Yamamoto K  Yoshikawa M  Zolensky ME 《Science (New York, N.Y.)》2011,333(6046):1116-1119

Meteorite studies suggest that each solar system object has a unique oxygen isotopic composition. Chondrites, the most primitive of meteorites, have been believed to be derived from asteroids, but oxygen isotopic compositions of asteroids themselves have not been established. We measured, using secondary ion mass spectrometry, oxygen isotopic compositions of rock particles from asteroid 25143 Itokawa returned by the Hayabusa spacecraft. Compositions of the particles are depleted in (16)O relative to terrestrial materials and indicate that Itokawa, an S-type asteroid, is one of the sources of the LL or L group of equilibrated ordinary chondrites. This is a direct oxygen-isotope link between chondrites and their parent asteroid.  相似文献   

Molecular and biological characterization of <Emphasis Type="Italic">Chrysanthemum stem necrosis virus</Emphasis> isolates from distinct regions in Japan     

Minoru?TakeshitaEmail author  Naoko?Nagai  Mitsuru?Okuda  Shohei?Matsuura  Shiori?Okuda  Naruto?Furuya  Kenichi?Tsuchiya 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(1):9-14

Three isolates of Chrysanthemum stem necrosis virus (CSNV) were obtained from chrysanthemum plants in distinct regions of Japan in 2006 and 2007. All the original host plants showed severe necrotic symptoms on the leaves and stems. Amino acid sequence data of the nucleocapsid protein genes of the three isolates (CbCh07A, TcCh07A, and GnCh07S) showed high identities with those of two other CSNV isolates, HiCh06A L1 from Japan and Chry1 from Brazil. Furthermore, for the first time the complete nucleotide sequence of the S RNA was determined for CSNV (isolate HiCh06A). In phylogenetic analysis based on the non-structural protein genes from the genus Tospovirus, HiCh06A L1 was placed in the same genetic group as Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus. Host range examination for isolates HiCh06A L1 and CbCh07A showed that green pepper (cv. ‘Kyoyutaka’, ‘Saitamawase’, ‘Tosakatsura’, ‘L3 sarara’ and ‘L3 miogi’) and tomato (cv. ‘Sekaiichitomato’) were systemically susceptible hosts, whereas TSWV-resistant Solanaceae species, Capsicum chinense, Lycopersicon peruvianum and a TSWV-resistant cultivar of green pepper (cv. TSR miogi), were resistant.  相似文献   

Acylated and non-acylated flavonol monoglycosides from the Indian minor spice Nagkesar (Mammea longifolia)     

Jagan Mohan Rao L  Yada H  Ono H  Yoshida M 《Journal of agricultural and food chemistry》2002,50(11):3143-3146

A methanol extract of nagkesar (buds of Mammea longifolia), which showed strong radical scavenging activity, yielded 13 compounds by separations using column chromatography and HPLC. Structure elucidation of these compounds was achieved by (1)H and (13)C NMR, including DQF-COSY, TOCSY, DEPT, HMQC, HSQC, and HMBC. They include two new compounds, quercetin 3-O-(2' ',4' 'di-E-p-coumaroyl)-alpha-L-rhamno-pyranoside and quercetin 3-O-(3' ',4' '-di-E-p-coumaroyl)-alpha-L-rhamnopyranoside, along with known compounds kaempferol, quercetin, the isopropylidenedioxy derivative of shikimic acid, kaempferol 3-O-(2' ',4' '-di-E-p-coumaroyl)-alpha-L-rhamnopyranoside, kaempferol 3-O-(3' ',4' '-di-E-p-coumaroyl)-alpha-L-rhamnopyranoside, kaempferol 3-O-alpha-L-rhamnopyranoside, quercetin 3-O-alpha-L-rhamnopyranoside, shikimic acid, kaempferol 3-O-beta-D-glucopyranoside, quercetin 3-O-beta-D-glucopyranoside, and beta-sitosterol 3-O-beta-D-glucopyranoside.  相似文献   

Effect of the strain combination of the donor and recipient on the production efficiency of W‐bearing sperm in mixed‐sex germline chimeric chickens     

Hiroshi KAGAMI  Jun YASUDA  Takahiro TAGAMI  Mitsuru NAITO  Yuko MATSUBARA  Takashi HARUMI  Takamasa NOGUCHI  Yasuhiro YAMAMOTO  Taikai TAKAHASHI  Junichi MATSUYAMA  Hiroyuki KOMATSU  Tamao ONO 《Animal Science Journal》2002,73(6):453-456

Effect of the strain combination of the donor and recipient on production efficiency of W‐bearing sperm in mixed‐sex chimeric testes was analyzed. The combinations of the donors and recipients were White Leghorn (WL) and Rhode Island Red (RIR), and vice versa. Generated mixed‐sex chimeras that had the male phenotype at sexual maturity were classified into four groups: (1) a female WL donor and a male RIR recipient; (2) a male WL donor and a female RIR recipient; (3) a female RIR donor and a male WL recipient; (4) a male RIR donor and a female WL recipient. The mean number of W‐bearing sperm detected by in situ hybridization among 10 000 sperm observed were 147, 165, 30 and 45 in groups 1, 2, 3 and 4, respectively. The numbers in groups 1 and 2 were both significantly higher than those of groups 3 and 4 (P < 0.05). The combination of a WL donor and a RIR recipient produced W‐bearing sperm more efficiently than the reverse combination.  相似文献   

Gastric adenocarcinoma with ossification in a ferret (Mustela putorius furo)     

Nakanishi M  Kuwamura M  Yamate J  Fujita D  Sasai H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(9):939-941

A 6-year-old female ferret had a firm mass 2 cm in diameter in the pyloric region of the stomach. Histopathologically, the mass was composed of neoplastic proliferation of well-differentiated epithelial cells, showing tubular or glandular growth patterns. Osseous metaplastic foci were often found in the tumor. Tumor cells showed a positive reaction for immunohistochemistry against bone morphogenetic protein-6, an osteogenic factor. A diagnosis of gastric adenocarcinoma with ossification was made.  相似文献   

Detection of the esp gene in high-level gentamicin resistant Enterococcus faecalis strains from pet animals in Japan   总被引：1，自引：0，他引：1  

Harada T  Tsuji N  Otsuki K  Murase T 《Veterinary microbiology》2005,106(1-2):139-143

We investigated the prevalence of the esp gene and the susceptibility to gentamicin in Enterococcus faecalis and E. faecium strains obtained from pet animals. Nine of 30 E. faecalis and 2 of 38 E. faecium strains from the pet animals had the esp gene. Three esp-positive E. faecalis strains, which were isolated from two dogs and a cat, showed gentamicin MICs of > or =256 microg/ml and harbored the high-level gentamicin resistance (HLGR) gene, aac(6')-Ie-aph(2')-Ia. Of the nine esp-positive E. faecalis strains, five, including the three strains with the HLGR gene, were closely related by numerical analysis of PFGE patterns. Longitudinal investigation needs to elucidate whether the HLGR gene was incorporated into a subpopulation of the esp-positive E. faecalis.  相似文献   

Calcium concentrations in maternal and fetal blood and in amniotic fluid from pregnant rats     

I Harada  K Ono  A Hasegawa  I Tomoda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1984,46(2):239-242

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