Ultrastructural changes in gill chloride cells during smoltification in wild and hatchery-reared masu salmon Oncorhynchus masou     

Shinya Mizuno  Kazuhiro Ura  Tadashi Okubo  Yuka Chida  Naoyuki Misaka  Shinji Adachi and  Kohei Yamauchi 《Fisheries Science》2000,66(4):670-677

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Morphological changes in juxtaglomerular cells of the kidney during smoltification in masu salmon Oncorhynchus masou     

Shinya Mizuno  Naoyuki Misaka  Noboru Kasahara 《Fisheries Science》2001,67(3):538-540

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Equine haptoglobin: isolation, characterization, and the effects of ageing, delivery and inflammation on its serum concentration.     

T Taira  T Fujinaga  M Okumura  K Yamashita  N Tsunoda  S Mizuno 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(3):435-442

Haptoglobin (Hp) was isolated from equine serum by ammonium sulphate precipitation, anion-exchange chromatography and gel filtration. Equine Hp which migrated to the alpha 2-globulin region in electrophoresis, contained 2 fractions with molecular weights (NW) of 108,000 and 105,000, and each fraction consisting of 2 subunits. Quantitative measurement of Hp in equine serum was performed by the single radial immunodiffusion method using anti-equine Hp serum. In clinically normal horses, the highest concentration of serum Hp was found in newborn foals and a high value was maintained until 12 months of age. The concentration then decreased with age. Normal Hp values were 5.25 +/- 2.36 mg/ml in foals (less than or equal to 12 months old), 2.19 +/- 1.54 mg/ml in adult horses (greater than or equal to 18 months old) and 3.62 +/- 0.81 mg/ml in all horses. Serum Hp concentration in mares during the perinatal period in comparison with the normal adult female was high for 4 months pre-partum, a passing increase at delivery, and then decreased at 2 weeks post-partum returning to normal within 1 month of delivery. In horses with experimentally-induced inflammation, serum Hp concentration began to increase immediately after treatment and reached the highest value, 1.5 to 9 times higher than those of pre-treatment at 2 to 5 days, then decreased within 4 weeks. It was also elevated in most cases of horses with clinically inflammatory signs.  相似文献   

Efficacy of single application ammonium sulfate in suppressing potato common scab     

Naoharu Mizuno  Hozumi Yoshida  Toshiaki Tadano 《Soil Science and Plant Nutrition》2013,59(3):611-616

Abstract

The objective of this study was to suppress potato common scab by lowering the soil pH and increasing the concentration of water-soluble aluminum (Al) in soil with a single application of ammonium sulfate into each row. Superphosphate (P) and potassium sulfate (K) were applied to the surface soil horizon and ammonium sulfate (N) was applied only into the rows along which potato plants were to be planted. By this application method, the soil pH was lowered and the concentration of water-soluble Al was increased in the soil of the rows where potato tubers were grown. Potato common scab was suppressed in the soil containing water-soluble Al in concentrations of 0.2 to 0.3 mg L^?1 or higher. The pH of the soil fertilized as indicated above remained lower than that of the control soil to which the mixture of N, P, and K was uniformly applied. In soil types such as Haplic Andosols containing allophane at high concentrations of 71 g kg^?1 in Memanbetsu, the suppression of potato common scab by this single application of ammonium sulfate was less effective due to the low soluble Al concentration. In other soil types, the soil pH was easily controlled and common scab was suppressed by this method. The advantages of this method are that (a) it minimizes the use of fertilizers, thus reducing the adverse effects of unnecessary fertilizers on the soil; and (b) it lowers the cost by eliminating additional agricultural chemicals and extra fertilizers.  相似文献   

A rice FRD3-like (OsFRDL1) gene is expressed in the cells involved in long-distance transport     

Haruhiko Inoue  Daichi Mizuno  Michiko Takahashi  Hiromi Nakanishi  Satoshi Mori  Naoko K. Nishizawa 《Soil Science and Plant Nutrition》2013,59(7):1133-1140

We identified four putative AtFRD3-like genes (OsFRDL) in the rice genome that exhibited 39.1 to 56.7% amino acid sequence similarities to Arabidopsis FRD3. Of these, we cloned three OsFRDL genes from a cDNA library prepared from iron-deficient rice roots: OsFRDL1, OsFRDL2, and OsFRDL3. OsFRDL1 was expressed weakly in Fe-sufficient roots, and slight expression was induced in the roots of Fe-deficient plants. OsFRDL2 was expressed constitutively in both roots and leaves, and Fe deficiency reduced its expression in leaves. OsFRDL3 was expressed in leaves, but not in roots; Fe deficiency induced slight expression in leaves. An OsFRDL1-sGFP fusion protein was localized in the plasma membrane in onion epidermal cells. The promoter GUS analysis showed that OsFRDL1 was localized in the cells involved in long-distance transport, in both Fe-sufficient and Fe-deficient plants. Furthermore, OsFRDL1 expression was observed during the reproductive stage. These results suggest that OsFRDL1 is a transporter that resides in the plasma membrane of cells involved in long-distant transport.  相似文献   

Structure-activity relationships of α-, β(1)-, γ-, and δ-tomatine and tomatidine against human breast (MDA-MB-231), gastric (KATO-III), and prostate (PC3) cancer cells     

Choi SH  Ahn JB  Kozukue N  Kim HJ  Nishitani Y  Zhang L  Mizuno M  Levin CE  Friedman M 《Journal of agricultural and food chemistry》2012,60(15):3891-3899

Partial acid hydrolysis of the tetrasaccharide (lycotetraose) side chain of the tomato glycoalkaloid α-tomatine resulted in the formation of four products with three, two, one, and zero carbohydrate side chains, which were separated by high-performance liquid chromatography (HPLC) and identified by thin-layer chromatography (TLC) and liquid chromatography ion-trap time-of-flight mass spectrometry (LCMS-IT-TOF). The inhibitory activities in terms of IC(50) values (concentration that inhibits 50% of the cells under the test conditions) of the parent compound and the hydrolysates, isolated by preparative HPLC, against normal human liver and lung cells and human breast, gastric, and prostate cancer cells indicate that (a) the removal of sugars significantly reduced the concentration-dependent cell-inhibiting effects of the test compounds, (b) PC3 prostate cancer cells were about 10 times more susceptible to inhibition by α-tomatine than the breast and gastric cancer cells or the normal cells, (c) the activity of α-tomatine against the prostate cancer cells was 200 times greater than that of the aglycone tomatidine, and (d) the activity increased as the number of sugars on the aglycone increased, but this was only statistically significant at p < 0.05 for the normal lung Hel299 cell line. The effect of the alkaloids on tumor necrosis factor α (TNF-α) was measured in RAW264.7 macrophage cells. There was a statistically significant negative correlation between the dosage of γ- and α-tomatine and the level of TNF-α. α-Tomatine was the most effective compound at reducing TNF-α. The dietary significance of the results and future research needs are discussed.  相似文献   

Long‐term outcome in dogs undergoing mitral valve repair with suture annuloplasty and chordae tendinae replacement     

T. Mizuno  T. Mizukoshi  M. Uechi 《The Journal of small animal practice》2013,54(2):104-107

Mitral valve repair under cardiopulmonary bypass was performed in three dogs with clinical signs associated with mitral regurgitation that were not controlled by medication. Mitral valve repair comprised circumferential annuloplasty and chordal replacement with expanded polytetrafluoroethylene. One dog died 2 years after surgery because of severe mitral regurgitation resulting from partial circumferential suture detachment. The others survived for over 5 years, but mild mitral valve stenosis persisted in one. The replaced chordae did not rupture in any dog. Mitral valve repair appears to be an effective treatment for mitral regurgitation in dogs. Chordal replacement with expanded polytetrafluoroethylene is a feasible technique, demonstrating long‐term durability in dogs. However, mitral annuloplasty techniques need improvement.  相似文献   

Clinical observations during induction and recovery of xylazine-midazolam- propofol anesthesia in horses     

Oku K  Yamanaka T  Ashihara N  Kawasaki K  Mizuno Y  Fujinaga T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(7):805-808

To evaluate clinical usefulness of xylazine (1.0 mg/kg)-midazolam (20 microg/kg)-propofol (3.0 mg/kg) anesthesia in horses, 6 adult Thoroughbred horses were examined. The quality of induction varied from poor to excellent and 5 out of 6 horses presented myotonus in the front half of the body. However, paddling immediately after induction observed in other reports of equine propofol anesthesia was not observed. Recovery time was 35.3 +/- 9.3 min and the quality of recovery was calm and smooth in all horses. Respiration rate decreased after induction and hypoxemia was observed during lateral recumbency. Heart rate also decreased after induction, however mean arterial blood pressure was maintained above approximately 100 mmHg.  相似文献   

Isolation of enterotoxin-producing Clostridium sporogenes from cefmetazole-associated diarrheic rabbit.     

Y Hara  S Igimi  F Kasuga  A Ogura  Y Nagaoka  S Takahashi  S Mizuno 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(3):531-532

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Propofol-ketamine anesthesia for internal fixation of fractures in racehorses   总被引：2，自引：0，他引：2  

Ohta M  Oku K  Mukai K  Akiyama K  Mizuno Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1433-1436

To assess the clinical usability of propofol-ketamine anesthesia for internal fixation of fractures in racehorses, hemodynamics, blood pH and gases, and vital responses to the continuous intravenous anesthesia in 7 surgical cases were analyzed. The quality of induction with propofol was variable for individual horses. The vital signs reflecting circulation, breath, and anesthetic depth were kept good without any troubles throughout the surgery. Mean time from the end of anesthesia to standing up was prolonged, however recovery from anesthesia was calm and smooth in all cases. Propofol-ketamine anesthesia may be a clinically usable technique for internal fixation of fractures in racehorses, however induction with propofol alone is not recommended.  相似文献