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11.
We investigated the effect of intravalvular liquid loss on changes in Escherichia coli (E. coli) levels in mussels (Mytilus galloprovincialis) harvested in different seasons and refrigerated (5°C) for 72 hours. A positive effect of seawater temperature at the time of harvesting on intravalvular liquid loss was found. No changes in E. coli levels in mussels (flesh and intravalvular liquid), as the result of intravalvular liquid loss, were observed. Levels of E. coli in the flesh decreased, probably as a result of cells dying off, causing the maintenance of E. coli levels in flesh and intravalvular liquid to be stable throughout storage.  相似文献   
12.
The understanding of the interactive effect of water and N availability, associated with the ability of crops to efficiently use these resources, is a crucial issue for stabilizing cereal production in Mediterranean areas. A 3-year side by side experiment on durum wheat and barley, under different water regimes and nitrogen levels, was carried out in a typical Mediterranean environment of Southern Italy, to identify the outstanding features of these species that contribute to enhanced grain yield and improved water and nitrogen use efficiency.  相似文献   
13.
Citrus tristeza virus (CTV) represents one of the major threats to citrus production worldwide. In the East Adriatic region, CTV symptoms are mostly absent due to traditional citrus grafting on trifoliate orange (Poncirus trifoliata), a CTV-tolerant rootstock. Therefore, the virus has been continuously spreading by the propagation of infected material. The genetic variability of CTV was studied on nineteen citrus samples, collected from orchards in the coastal region of Croatia, Montenegro and Albania, that previously tested positive by ELISA and immunocapture RT-PCR. Single-strand conformation polymorphism of the amplified coat protein gene demonstrated the presence of different CTV variants in each amplicon, while sequence analysis of cloned CP gene variants confirmed their clustering into six out of the seven phylogenetic groups so far delineated. Four of these groups include sequences of severe quick decline, seedling yellows and stem-pitting (SP) isolates, thought to be found only rarely in the Mediterranean region. Regardless of the lack of symptoms in the field, CTV isolates from the East Adriatic displayed high genetic variability and pathogenic potential, additionally confirmed by biological characterisation. The high percentage of mixed infections suggest the potential for further diversification and a greater risk of severe variants spreading into new areas.  相似文献   
14.

Background

The abolition of in-feed antibiotics or chemotherapeutics as growth promoters have stimulated the swine industry to look for alternatives such as organic acids, botanicals, probiotics and tannin. The objective of the present study was to compare the effects of a combination of acids and tannin with diet with organic acids and diet without growth promoters on the growth performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs. Tannin is more natural and cheaper but possibly with the same effectiveness as organic acids with regard to growth performance.

Methods

Thirty-six 7 week old grower pigs, divided into three equal groups, were used in a three week feeding trial. Group I was fed basal diet, group II basal diet with added organic acids and group III basal diet with added organic and inorganic acids and tannin. Pigs were weighed before and after feeding and observed daily. Blood was collected before and after the feeding trial for the determination of selected biochemical, haematological and antioxidant enzyme parameters. One-way ANOVA was used to assess any diet related changes of all the parameters. Paired t-test was used to evaluate changes of blood parameters individually in each group of growers before and after feeding.

Results

No clinical health problems related to diet were noted during the three week feeding trial. The average daily gain (ADG) and selected blood parameters were not affected by the addition to basal diet of either acids and tannin or of organic acids alone. Selected blood parameters remained within the reference range before and after the feeding trial, with the exception of total serum proteins that were below the lower value of reference range at both times. The significant changes (paired t-test) observed in individual groups before and after the feeding trial are related to the growth of pigs.

Conclusion

Diet with acids and tannin did not improve the growth performance of grower pigs but had no deleterious effects on selected blood parameters. The possibility of beneficial effects of adding acids and tannin in diets on growth performance over a longer period, however, could not be excluded.  相似文献   
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The comparative effectiveness of 2-phenoxyethanol and Propiscin as anesthetics were studied on juvenile sea bass. Both anesthetics showed low toxicity and good hypnotic characteristics, however median survival times at the same concentration of both anesthetics differed. Propiscin showed much lower LT50 than 2-phenoxyethanol, pointing towards its elevated toxicity in juvenile sea bass, even the same concentrations of Propiscin induced slower anesthesia than 2-phenoxyethanol. The most suitable Propiscin concentration for anesthesia was 0.56 ml/l, while the most suitable concentration of 2-phenoxyethanol was 0.32 ml/l for juvenile sea bass. Mortalities after 96 h were induced with Propiscin at lower concentrations then of 2-phenoxyethanol, resulting in the conclusion that the 2-phenoxyethanol is a safer anesthetic for use with juvenile sea bass (Dicentrarchus labrax L.).  相似文献   
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