首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   406篇
  免费   21篇
林业   38篇
农学   19篇
基础科学   4篇
  97篇
综合类   37篇
农作物   41篇
水产渔业   54篇
畜牧兽医   77篇
园艺   4篇
植物保护   56篇
  2022年   17篇
  2021年   19篇
  2020年   17篇
  2019年   19篇
  2018年   34篇
  2017年   24篇
  2016年   26篇
  2015年   22篇
  2014年   16篇
  2013年   34篇
  2012年   39篇
  2011年   37篇
  2010年   18篇
  2009年   15篇
  2008年   16篇
  2007年   7篇
  2006年   7篇
  2005年   5篇
  2004年   5篇
  2003年   5篇
  2002年   8篇
  2001年   2篇
  2000年   5篇
  1999年   4篇
  1998年   3篇
  1997年   2篇
  1996年   3篇
  1995年   2篇
  1993年   2篇
  1988年   2篇
  1984年   2篇
  1982年   1篇
  1980年   1篇
  1976年   1篇
  1974年   2篇
  1973年   2篇
  1972年   1篇
  1967年   2篇
排序方式: 共有427条查询结果,搜索用时 31 毫秒
51.
【目的】研究退化伊犁绢蒿荒漠草地恢复后,主要植物的生态化学计量特征的变化,为干旱区草地恢复提供理论依据。【方法】以该草地的建群种伊犁绢蒿(Seriphidium transiliense)和伴生种木地肤(Kochia prostrata)为对象,分析两种植物的C、N、P、K含量及计量比以及放牧和封育条件下,两种植物的生态化学计量特征,研究两种植物的养分利用策略以及封育对两种植物的生态化学计量特征的影响。【结果】伊犁绢蒿的P(0.82 g/kg)极显著高于木地肤的P(0.58 g/kg),伊犁绢蒿的N(15.11 g/kg)、K(13.06 g/kg)含量和N/P(22.46)极显著低于木地肤的N(25.14 g/kg)、K(21.17 g/kg)含量和N/P(43.75)。封育改善了荒漠草地植物的生长环境,但与放牧相比,伊犁绢蒿与木地肤的C、N、P、K含量及元素计量比变化不显著。【结论】伊犁绢蒿与木地肤的养分利用策略不同,伊犁绢蒿以吸收利用P为主,木地肤以吸收利用N和K为主,伊犁绢蒿的生长率高于木地肤。封育对荒漠草地两种植物的生态化学计量特征影响不显著。  相似文献   
52.
Malaysian Mahseer (Tor tambroides) has a good prospect for aquaculture because of its high market demand. However, there is a scarce information on gut microbiota associated with Malaysian Mahseer unlike other fish species. Therefore, we constructed and compared gut microbiota in different developmental stages (larval, juvenile, fingerling, yearling, and adult) using culture dependent and PCR‐DGGE fingerprinting technique for better understanding of gut microbiota composition associated with T. tambroides. Culturable gut microbiota composition in all developmental stages were composed of β‐ and γ‐Proteobacteria, and Bacilli. Biodiversity analysis of culturable gut microbiota showed that larval, juvenile, and adult stages have higher diversity than fingerling and yearling stages. Ward's linkage cluster analysis showed that culturable gut microbiota composition in larval and juvenile stages were close to adult stages, whereas fingerling and yearling stage composed same cluster. PCR‐DGGE fingerprinting technique showed that unculturable gut microbiota were constituted by α‐and γ‐Proteobacteria, and Actinobacteria. Ward's linkage cluster analysis showed that unculturable gut microbiota composition in both larval and juvenile stages were distinct from other developmental stages. Our results revealed that gut microbiota composition were varied in different developmental stages of Malaysian Mahseer and continuous shifts of gut microbiota from larval to adult stages.  相似文献   
53.
Aerobic rice technology is still new in Malaysia, and information regarding MARDI Aerob 1 (MA1), the first local aerobic rice variety, is still lacking. Therefore, comparative studies were carried out to determine the physiological performance of aerobic rice variety MA1 and lowland rice variety MR253 under water stress given at the panicle initiation, flowering and ripening stages. This experiment was arranged in a randomized complete block design. Stomatal conductance (gs), chlorophyll a fluorescence (Fv/Fm), leaf relative water content (leaf RWC), and soil moisture content (SMC) as well as yield component parameters such as panicle number, grain yield and 100-grain weight were measured. Results revealed that gs and leaf RWC for both varieties decreased with depletion of SMC. The correlation study between the physiological parameters and SMC indicated that Fv/Fm was not affected by water stress, regardless of varieties. The yield components (panicle number, grain yield and 100-grain weight) for both varieties greatly decreased when water stress was imposed at the panicle initiation stage. This study showed that the panicle initiation period was the most sensitive stage to water stress that contributed to a substantial reduction in yield for both varieties. Under the aerobic condition (control), MR253 produced higher panicle number, 100-grain weight and yield than MA1. Although MR253 is bred for lowland, it is well adapted to aerobic condition.  相似文献   
54.
The efficacy of antimicrobial treatment of cotton fabrics depends on various parameters of the coating process, such as the chemical nature and concentration of the antimicrobial agent, the composition of the crosslinking formulation, and the curing temperature. The inclusion complex of triclosan with β-cyclodextrin (βCD) was synthesized and characterized by FTIR, XRD, NMR, Raman, SEM, and TGA. The minimum inhibitory concentration and minimum bactericidal concentration of the complex against Klebsiella pneumoniae and Staphylococcus aureus were compared to those of its precursor. A multifactorial study included an evaluation of the effects of triclosan complexation with β-cyclodextrin, a comparison between the glyoxal and tetracarboxylic acid as crosslinkers, an investigation of the effect of crosslinker and catalyst concentrations, and a comparison of curing at 120°C and 180°C. The cotton was characterized by FTIR-ATR, the micrographs of treated samples were obtained by SEM and the weight add-on was calculated. The bactericidal properties were determined according to AATCC-147. The correlation between the coating process parameters and the antimicrobial efficacy was determined. The optimal combination leading to the highest weight add-on and the antimicrobial coating that was most durable to multiple detergent washes at an elevated temperature was the use of complexed triclosan grafted onto the cotton in the presence of tetracarboxylic acid, followed by curing at 180°C. The curing temperatures were 120°C (P=0.002) and 180°C (P=0.008), catalysts were 1 % and 2 % aluminium sulfate and sodium hypophosphite (P<0.001), and the crosslinkers were 5 % and 10 % glyoxal and butanetetracarboxylic acid (P<0.001); these parameters significantly enhanced the antimicrobial properties of the treated fabrics. The study showed that βCD did not have antimicrobial activity, while the βCD/triclosan-treated textile exhibited potential antimicrobial properties. Overall, the bactericidal activity of fabrics can be enhanced by using βCD/triclosan with 10 % butanetetracarboxylic acid as a cross-linker and 5 % sodium hypophosphite as a catalyst at a curing temperature of 180°C.  相似文献   
55.

Background

Fungal endophytes are the living symbionts which cause no apparent damage to the host tissue. The distribution pattern of these endophytes within a host plant is mediated by environmental factors. This study was carried out to explore the fungal endophyte community and their distribution pattern in Asparagus racemosus and Hemidesmus indicus growing in the study area.

Results

Foliar endophytes were isolated for 2 years from A. racemosus and H. indicus at four different seasons (June–August, September–November, December–February, March–May). A total of 5400 (675/season/year) leaf segments harbored 38 fungal species belonging to 17 genera, 12 miscellaneous mycelia sterile from 968 isolates and 13 had yeast like growth. In A. racemosus, Acremonium strictum and Phomopsis sp.1, were dominant with overall relative colonization densities (RCD) of 7.11% and 5.44% respectively, followed by Colletotrichum sp.3 and Colletotrichum sp.1 of 4.89% and 4.83% respectively. In H. indicus the dominant species was A. strictum having higher overall RCD of 5.06%, followed by Fusarium moniliforme and Colletotrichum sp.2 with RCD of 3.83% and 3%, respectively. Further the overall colonization and isolation rates were higher during the wet periods (September–November) in both A. racemosus (92.22% and 95.11%) and H. indicus (82% and 77.11%).

Conclusion

Study samples treated with 0.2% HgCl2 and 75% EtOH for 30 s and 1 min, respectively, confirmed most favorable method of isolation of the endophytes. Owing to high mean isolation and colonization rates, September–November season proved to be the optimal season for endophyte isolation in both the study plants. Assessing the bioactive potential of these endophytes, may lead to the isolation of novel natural products and metabolites.
  相似文献   
56.
The successful isolation and characterization of water hyacinth fiber (Eichornia crassipes) (WHF) nanocellulose is presented in this study. The novelty was in exploring a wider range of properties of highly purified samples of WHF after each stage of production in more depth. The isolation was accomplished by pulping in a digester and sonication. Morphological changes before and after treatment were demonstrated by scanning electron microscopy (SEM). The lignin and hemicellulose content decreased during chemical treatment. Transmission electron microscopy (TEM) and particle size analyzer (PSA) were used to determine the morphology of WHF after sonication for 1 h. TEM shows that the diameter and length of nanocellulose WHF were 15.61 and 147.4 nm, respectively. The crystallinity index and crystalline domain area significantly increased after chemical treatment. The highest crystallinity index was 84.87 % after an acid hydrolysis process. The increase in crystallinity leads to good thermal stability. Moisture absorption tests of WHF were carried out before and after treatment. The lowest moisture absorption was in the cellulose fiber after sonication (nanocellulose).  相似文献   
57.
Present study dealt with the fabrication of chitosan/zinc oxide nano-composites (CZNCs) using a facile preparation method. The structural features of nano-composites were investigated by using advanced analytical techniques. The developed nano-composites exhibited hexagonal structural pattern with an average particle size of about 51 nm. The developed CZNCs were dispersed in 2-propanol and applied on polyester by using the pad-dry-cure method. The treated fabric specimens were characterized for surface, functional and textile properties including antibacterial activity and (ultra violet) UV- blocking. The nano-composite treated polyester fabric exhibited durable antibacterial, UV- blocking and textile properties with fair whiteness index.  相似文献   
58.
59.
Cryptocaryonosis is a major problem for mariculture, and the absence of suitable sero‐surveillance tools for the detection of cryptocaryonosis makes it difficult to screen Cryptocaryon irritans‐infected fish, particularly asymptomatic fish. In this study, we proposed a serum‐based assay using selected C. irritans proteins to screen infected and asymptomatic fish. Eight highly expressed genes were chosen from an earlier study on C. irritans expressed sequence tags and ciliate glutamine codons were converted to universal glutamine codons. The chemically synthesized C. irritans genes were then expressed in an Escherichia coli expression host under optimized conditions. Five C. irritans proteins were successfully expressed in E. coli and purified by affinity chromatography. These proteins were used as antigens in an enzyme‐linked immunosorbent assay (ELISA) to screen sera from experimentally immunized fish and naturally infected fish. Sera from both categories of fish reacted equally well with the expressed C. irritans recombinant proteins as well as with sonicated theronts. This study demonstrated the utility of producing ciliate recombinant proteins in a heterologous expression host. An ELISA was successfully developed to diagnose infected and asymptomatic fish using the recombinant proteins as antigens.  相似文献   
60.
This paper examines the influences of Malaysia's Federation Land Development Authority (FELDA) scheme in fostering inclusive rural development. Based on the model of holistic inclusive development, the paper investigates the performance of FELDA from four perspectives: social development, industrialisation, modernisation and basic needs. The main findings of the study indicate that, to a large extent, the scheme has successfully stimulated both the social and economic development of the community. Nonetheless, establishing a strong trust, social cohesion and rapport between the public authorities and community remain the main challenges in determining the success of this state‐led agenda. Moreover, keeping traditional values in the modern system will be the key principle for the sustainability of the programme if plans are made to adopt the scheme in other regions.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号