Amino Acid Composition of Developing Larval Freshwater Prawn Macrobrachium rosenbergii   总被引：1，自引：0，他引：1  

Paymon  Roustaian Mohd Salleh  Kamarudin Hishamuddin  Omar Che Roos  Saad Mansor Haji  Ahmad 《Journal of the World Aquaculture Society》2000,31(1):130-136

Abstract.— Amino acid profiles of freshwater prawn Macrobrachium rosenbergii were determined during early larval stages (I-IX) to provide some baseline biochemical information of the growing larvae. The larvae obtained from several females were stocked into three 250-L tanks at a density of 30 larvae/L. The feeding regime consisted of newly hatched Artemia nauplii and egg custard containing 5% cod liver oil. For each developmental stage, larval samples from each experimental tank were pooled, freeze dried and after acid hydrolysis, the quantification of amino acids was done by a reverse phase high performance liquid chromatography ( N = 2). The highest represented of the total amino acids were for glutamic acid and phenylalanine (with cystine) with ranges from 13.4–16.6 and 9.7–11.5%, respectively, whereas tryptophan (1.4–1.6%). methionine (1.4–2.7%) and histidine (2.9–4.2%) were relatively lower. The levels of the following essential amino acids did not significantly change during larval development: arginine, leucine, phenylalanine (plus cystine), threonine, tryptophan and valine. Despite statistically significant changes that were observed in levels of some amino acids, amino acid composition appeared to be relatively unchanged during the larval growth. The results may suggest that the amino acid requirements of the freshwater prawn is relatively constant during larval life and can be satistied by a suitable protein source that resembles the larval amino acid profile.  相似文献   

Morphology,phylogeny and pathogenicity of Fusarium species from Sansevieria trifasciata in Malaysia     

Yee Jia Kee  Latiffah Zakaria  Masratul Hawa Mohd 《Plant pathology》2020,69(3):442-454

Leaf blight is a common disease affecting Sansevieria trifasciata in many countries, including Malaysia. In the present study, Fusarium isolates were consistently recovered from the diseased leaves collected from various locations throughout the country. Based on morphology and multigene phylogenetic analysis using mitochondrial small subunit (mtSSU), intergenic spacer region (IGS) and translation elongation factor 1-α (TEF1-α) gene sequences, seven Fusarium species were identified, with F. oxysporum being the most prevalent (67.6%) among 34 isolates. Pathogenicity tests resulted in the discovery of pathogenic isolates that belonged to F. oxysporum, F. proliferatum, and F. pseudocircinatum, whereas all isolates of F. brachygibbosum, F. concentricum, F. mangiferae, and F. solani were nonpathogenic. The results suggest that several Fusarium species are accountable for causing disease on S. trifasciata in Malaysia.  相似文献   

Pathological changes,distribution and detection of Brucella melitensis in foetuses of experimentally-infected does     

Mazlina Mazlan  Siti Khairani-Bejo  Hazilawati Hamzah  Nurrul Shaqinah Nasruddin  Annas Salleh  Mohd Zamri-Saad 《The Veterinary quarterly》2021,41(1):36

BackgroundBrucellosis of goats is caused by Brucella melitensis. It is a re-emerging zoonotic disease in many countries due to transmission from domestic animals and wildlife such as ibex, deer and wild buffaloes.ObjectiveTo describe the pathological changes, identification and distribution of B. melitensis in foetuses of experimentally infected does.MethodsTwelve female goats of approximately 90 days pregnant were divided into 4 groups. Group 1 was exposed intra-conjunctival to 100 µL of sterile PBS while goats of Groups 2, 3 and 4 were similarly exposed to 100 µL of an inoculum containing 10⁹ CFU/mL of live B. melitensis. Goats of these groups were killed at 15, 30 and 60 days post-inoculation, respectively. Foetal fluid and tissues were collected for bacterial identification (using direct bacterial culture, PCR and immuno-peroxidase staining) and histopathological examination.ResultsBilateral intra-conjunctival exposure of pregnant does resulted in in-utero infection of the foetuses. All full-term foetuses of group 4 were either aborted or stillborn, showing petechiations of the skin or absence of hair coat with subcutaneous oedema. The internal organs showed most severe lesions. Immune-peroxidase staining revealed antigen distribution in all organs that became most extensive in group 4. Brucella melitensis was successfully isolated from the stomach content, foetal fluid and various other organs.ConclusionVertical transmission of caprine brucellosis was evident causing mild to moderate lesions in different organs. The samples of choice for isolation and identification of B. melitensis are stomach content as well as liver and spleen tissue.  相似文献   

Historical review and notes on the correct scientific name for seashore mangosteen     

M. Nazre 《Genetic Resources and Crop Evolution》2010,57(8):1249-1259

Taxonomic name for “seashore mangosteen” has been known as Garcinia hombroniana and was first established by Pierre (Flore Forestiere de la Cochinchine 1-2. pl. 54–98 Paris, 1882–1885). The distribution of this species is wide and can be found in the Malay Peninsula, upper part of Borneo, and in Andaman and Nicobar islands but not in the Indonesian islands. Instead, many other names are used in Indonesian islands for species that resembles G. hombroniana. This study reviews relevant taxonomic literatures accompanied by observation on herbarium specimens to see whether G. hombroniana is a valid taxonomic name to be used since many more species resembling it were used elsewhere. Historically, when Pierre created G. hombroniana, he placed it together with two Linnaeus species, G. celebica and G. cornea within section Kiras based on the characteristics of male flowers, fruits and geographical distribution. However, no surviving type specimens for G. celebica and G. cornea were found and this has created confusion on the true identities of both Linnaeus species, which have then resulted into many newly-established species. In addition, many early taxonomic classifications including the work of Linnaeus and Pierre have used poor characters for their species delimitation. Morphological evidences based on the literature and herbarium specimens suggested that there are no clear cut discontinuities in morphological characters or any characters exclusive to certain geographical areas between G. hombroniana with G. celebica and G. cornea. Because of that, the valid name that should be used is G. celebica as it was published much earlier. In addition to that, other names including G. benthamii, G. fabrilis, G. ferrea, G. rumphii, G. jawoera, G. kingii, G. krawang, G. kurzii and G. speciosa should simply be synonyms of G. celebica.  相似文献   

Alteration in lymphocyte responses,cytokine and chemokine profiles in chickens infected with genotype VII and VIII velogenic Newcastle disease virus     

Mehdi Rasoli  Swee Keong Yeap  Sheau Wei Tan  Hassan Moeini  Aini Ideris  Mohd Hair Bejo  Noorjahan Banu Mohamed Alitheen  Pete Kaiser  Abdul Rahman Omar 《Comparative immunology, microbiology and infectious diseases》2014

Newcastle disease (ND) is a highly contagious avian disease and one of the major causes of economic losses in the poultry industry. The emergence of virulent NDV genotypes and repeated outbreaks of NDV in vaccinated chickens have raised the need for fundamental studies on the virus–host interactions. In this study, the profiles of B and T lymphocytes and macrophages and differential expression of 26 immune-related genes in the spleen of specific-pathogen-free (SPF) chickens, infected with either the velogenic genotype VII NDV strain IBS002 or the genotype VIII NDV strain AF2240, were evaluated. A significant reduction in T lymphocyte population and an increase in the infiltration of IgM⁺ B cells and KUL01⁺ macrophages were detected in the infected spleens at 1, 3 and 4 days post-infection (dpi) (P < 0.05). The gene expression profiles showed an up-regulation of CCLi3, CXCLi1, CXCLi2 (IL-8), IFN-γ, IL-12α, IL-18, IL-1β, IL-6, iNOS, TLR7, MHCI, IL-17F and TNFSF13B (P < 0.05). However, these two genotypes showed different cytokine expression patterns and viral load. IBS002 showed higher viral load than AF2240 in spleen at 3 and 4 dpi and caused a more rapid up-regulation of CXCLi2, IFN-γ, IL-12α, IL-18, IL-1β, iNOS and IL-10 at 3 dpi. Meanwhile, the expression levels of CCLI3, CXCLi1, IFN-γ, IL-12α, IL-1β and iNOS genes were significantly higher in AF2240 at 4 dpi. In addition, the expression levels of IL-10 were significantly higher in the IBS002-infected chickens at 3 and 4 dpi. Hence, infection with velogenic genotype VII and VIII NDV induced different viral load and production of cytokines and chemokines associated with inflammatory reactions.  相似文献   

Physical Parameters and Risk Factors Associated with the Elimination of Arabian and Crossed Arabian Endurance Horses during a 120-km Endurance Race     

Lawan Adamu  Noraniza Mohd Adzahan  Abdullah Rasedee  Bashir Ahmad 《Journal of Equine Veterinary Science》2014

Endurance racing is one long-distance event among the equine sporting activities associated with severe injuries and metabolic derangements that impede the health status of horses participating in the event. Therefore, this study evaluated the physical parameters and risk factors associated with the elimination of Arabian and crossed Arabian endurance horses during a 120-km race. Fifty-three Arabian and crossed Arabian endurance horses aged 6 to 15 years old participated in an endurance race of 120 km. All the horses were examined and found fit according to Fédération Équestre Internationale (FEI) regulations. After the endurance race 14 Arabian endurance horses completed the race successfully while 31 Arabian and crossed Arabian were eliminated. Their speed and heart rates were significantly higher (P < .0001). There were significant differences in skin recoil (P < .0395), color of mucous membrane (P < .0189), intestinal motility (P < .0124), and capillary refill time (P < .0082). There also were significant differences in age (P < .0018), breed (P < .0119), and equine establishments (P < .0205) among the eliminated horses and the Arabian and crossed Arabian endurance horses that successfully completed the race. In conclusion, the physical parameters and risk factors were significantly associated with the elimination of Arabian and crossed Arabian endurance horses. Therefore, close monitoring of the physical parameters and risk factors are essential in order to improve the performance of Arabian and crossed Arabian horses, and further studies are required on other associated parameters.  相似文献   

Bovine brucellosis – a comprehensive review     

Sandip Kumar Khurana  Anju Sehrawat  Ruchi Tiwari  Minakshi Prasad  Baldev Gulati  Muhammad Zubair Shabbir  Rajesh Chhabra  Kumaragurubaran Karthik  Shailesh Kumar Patel  Mamta Pathak  Mohd. Iqbal Yatoo  Vivek Kumar Gupta  Kuldeep Dhama  Ranjit Sah  Wanpen Chaicumpa 《The Veterinary quarterly》2021,41(1):61

Brucellosis is a zoonotic disease of great animal welfare and economic implications worldwide known since ancient times. The emergence of brucellosis in new areas as well as transmission of brucellosis from wild and domestic animals is of great significance in terms of new epidemiological dimensions. Brucellosis poses a major public health threat by the consumption of non-pasteurized milk and milk products produced by unhygienic dairy farms in endemic areas. Regular and meticulous surveillance is essentially required to determine the true picture of brucellosis especially in areas with continuous high prevalence. Additionally, international migration of humans, animals and trade of animal products has created a challenge for disease spread and diagnosis in non-endemic areas. Isolation and identification remain the gold standard test, which requires expertise. The advancement in diagnostic strategies coupled with screening of newly introduced animals is warranted to control the disease. Of note, the diagnostic value of miRNAs for appropriate detection of B. abortus infection has been shown. The most widely used vaccine strains to protect against Brucella infection and related abortions in cattle are strain 19 and RB51. Moreover, it is very important to note that no vaccine, which is highly protective, safe and effective is available either for bovines or human beings. Research results encourage the use of bacteriophage lysates in treatment of bovine brucellosis. One Health approach can aid in control of this disease, both in animals and man.  相似文献   

The role of performance assessment in real time system management     

M. N. Mohd Adnan 《Irrigation and Drainage Systems》1993,7(4):273-289

This paper discusses paddy irrigation development in Malaysia in relation to real-time systems management and the role of performance assessment in such a system. The Muda Irrigation Scheme real-time management system is given as an illustration. This system has shown that, with performance assessment, managers are more prepared and able to respond quickly to issues faced in the day to day systems operations. In this system, demand-supply matching can be made faster and with improved utilisation of effective rainfall and uncontrolled flows. Opportunities for saving uncontrolled flows in the paddy fields can be identified in time. With dryseeding method, the system has been successful in sustaining production in severe water shortage situations. Into the 90s and beyond, no major infrastructure investments in irrigation systems are expected. Irrigation managers must be committed towards excellence in performance. This can be achieved through improved real-time performance assessment. Some aspects of performance assessment that could be improved are in its roles in meeting cropping intensity targets, accurate demand-supply matching, meeting water savings target and in alerting managers to potential crisis situations. Carefully designed and installed, performance assessment can also improve organisational and staff management.  相似文献   

Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC3F2 advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai     

Magaji G. Usman  Oladosu Yusuff  Mohammad Y. Martini  Mohd R. Ismail  Raihana Ridzuan 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2020,70(3):252-264

ABSTRACT

Domestic production of chilli in Malaysia is insufficient which accounted for 52% self-sufficiency level. Poor performance of local varieties under high temperatures above 42°C is one of the major factors affecting chilli production. This study was conducted with the objective of introgress heat-tolerant gene (Hsps) from AVPP0702 donor into Kulai using marker-assisted backcrossing. A total of 68 SSR markers, including Hsp linked markers that showed polymorphism between the parents were used to assess the generation of backcrossing; BC1F1, BC2F1, BC3F1 and BC3F2 and the average RPG percentage of the recurrent parent was found to be 81, 90, 95 and 97%, respectively. The pattern of Hsp expression in the backcross generations was similar to the donor parent (upregulated with more than 4-fold increase). Twelve improved heat tolerant chilli lines were developed. Most of the morphological and agronomical traits were recovered in the selected improved heat-tolerant genotypes from Kulai such as plant height, number of days to 50% flowering, number of fruits, fruit length and total fruit yield per plant. Improved high-yielding heat-tolerant chilli lines showed tolerance to high temperature as well as did not express any negative effect on agronomic traits in comparison with Kulai variety.  相似文献   

Inhibition of low-density lipoprotein oxidation and up-regulation of low-density lipoprotein receptor in HepG2 cells by tropical plant extracts   总被引：2，自引：0，他引：2  

Salleh MN  Runnie I  Roach PD  Mohamed S  Abeywardena MY 《Journal of agricultural and food chemistry》2002,50(13):3693-3697

Twelve edible plant extracts rich in polyphenols were screened for their potential to inhibit oxidation of low-density lipoprotein (LDL) in vitro and to modulate LDL receptor (LDLr) activity in cultured HepG2 cells. The antioxidant activity (inhibition of LDL oxidation) was determined by measuring the formation of conjugated dienes (lag time) and thiobarbituric acid reagent substances (TBARS). Betel leaf (94%), cashew shoot (63%), Japanese mint (52%), semambu leaf (50%), palm frond (41%), sweet potato shoot, chilli fruit, papaya shoot, roselle calyx, and maman showed significantly increased lag time (>55 min, P < 0.05) and inhibition of TBARS formation (P < 0.05) compared to control. LDLr was significantly up-regulated (P < 0.05) by Japanese mint (67%), semambu (51%), cashew (50%), and noni (49%). Except for noni and betel leaf, most plant extracts studied demonstrated a positive association between antioxidant activity and the ability to up-regulate LDL receptor. Findings suggest that reported protective actions of plant polyphenols on lipoprotein metabolism might be exerted at different biochemical mechanisms.  相似文献