Assessment of growth condition for a candidate probiotic, <Emphasis Type="Italic">Shewanella algae</Emphasis>, isolated from digestive system of a healthy juvenile <Emphasis Type="Italic">Penaeus monodon</Emphasis>     

Shahram Shakiba Zadeh  Che Roos Saad  Annie Christianus  Mohd Salleh Kamarudin  Kamaruzaman Sijam  Mariana Nor Shamsudin  Vasantha Kumari Neela 《Aquaculture International》2010,18(6):1017-1026

To conquer disease problem in shrimp industries, probiotic biocontrol is a well-known remedy now. The antagonistic ability of separated isolates from different parts of juvenile P. monodon was screened against shrimp Vibrio pathogens, V. parahaemolyticus and V. alginolyticus. The most antagonistic effect was observed for an isolate that primarily identified as Shewanella algae using conventional method followed by Biolog GN and GP microplates. Since adaptability to the host optimum cultural condition of the target organism is of the great importance, response surface methodology, with central composite design, was applied to assess log cell count response of S. algae in different incubation conditions. Therefore, four independent variables were assumed as: temperature (10–50°C), pH (6–10), NaCl concentration (0–50‰) and time (12–60 h). The coefficients of multiple determinations (R ²) for the responses log cell count of S. algae being 0.827. Temperature was the merely significant independent variable that affected the log cell count of the candidate probiotic. The candidate probiotic was revealed a reasonable growth response in quite wide range of temperature, pH and NaCl concentration in which the maximum levels were in same range of optimum shrimp culture.  相似文献   

Streptococcosis in red hybrid tilapia (Oreochromis niloticus) commercial farms in Malaysia     

Najiah Musa  Lee Seong Wei  Nadirah Musa  Ruhil H Hamdan  Lee Kok Leong  Wendy Wee  Mohd Nur Amal  Basiriah M Kutty  & Siti Zahrah Abdullah 《Aquaculture Research》2009,40(5):630-632

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Development of advanced fragrant rice lines from MR269 × Basmati 370 through marker-assisted backcrossing     

Wendy Chui Phing Lau  Mohd Y. Rafii  Mohd Razi Ismail  Adam Puteh  Mohammad Abdul Latif  Ramli Asfaliza  Gous Miah 《Euphytica》2017,213(1):11

Fragrance in rice is an appealing attribute to consumers. The increasing demand for fragrant rice highlights the need to develop fragrant rice variety that suit the preference of local consumers in addition to reduce fragrant rice imports. Marker-assisted backcrossing (MABC) was employed to develop advanced fragrant rice lines from the cross between MR269 and Basmati 370. MR269 is a Malaysian high-yielding rice variety but non-fragrant and was used as recurrent parent whereas Basmati 370 is a well-known fragrant traditional rice variety and was used as donor parent for the fragrance gene. Two generations of backcrosses and a generation of selfing were conducted to introgress the fragrance gene and restore the recurrent parent genome in the backcross progenies. As a result, 14 advanced fragrant rice lines were developed. These advanced fragrant rice lines carried homozygous alleles for the fragrance gene, similar to Basmati 370. The average recovery of recurrent parent genome was 88.4%. Besides being fragrant, the advanced fragrant rice lines also had most of the morphological and agronomical traits similar to MR269. Grain quality of the advanced fragrant rice lines in terms of gelatinization temperature, amylose content and gel consistency are also similar to both parents. Besides, the advanced fragrant rice lines had 2-acetyl-1-pyrroline content similar to Basmati 370. MABC approach applied in this study has successfully introgressed the fragrance gene and accelerated the recovery of recurrent parent genome in advanced fragrant rice lines, therefore these lines can be delivered to the farmers and consumers for use in due time.  相似文献