Electro-spun polyethylene terephthalate (PET) mat as a keratoprosthesis skirt and its cellular study     

Esmaeil Biazar  Mehrnaz Ahmadian  Saeed Heidari K  Akbar Gazmeh  Seyed-Farzad Mohammadi  Alireza Lashay  Mohsen Heidari  Hamed Eslami  Mohammadali Sahebalzamani  Hassan Hashemi 《Fibers and Polymers》2017,18(8):1545-1553

Artificial keratoprostheses are indispensable for visual rehabilitation in patients with end-stage corneal blindness. This study aimed to assess the biocompatibility of polyethylene terephthalate nanofibrous mats and its potential as a novel synthetic keratoprosthesis skirt material for corneal tissue engineering. Nanofibrous mats were prepared by an electrospinning method and were first treated with the CO₂ plasma to yield carboxylic groups on the surface; finally, the modified PET mat was cross linked with collagen using water-soluble carbodiimide as a coupling agent. The samples were evaluated by ATR-FTIR, scanning electron microscope (SEM), contact angle, and cell culture. The cross-linking of collagen on PET surface was confirmed by ATR-FTIR spectroscopy and SEM images The 79° difference was obtained in the contact angle analysis, obtained for the collagen-cross-linked nanofibrous mat than the non-modified nanofibrous mat. Cellular investigation showed limbal epithelial progenitor cells (LEPCs) has been better adhesion, cell growth, and proliferation of collagen-crosslinked nanofibrous samples than other samples. The bioavailability of PET fibers with covalently attached collagen was found to be identical to that of PET fibers with covalent attachment is a suitable method for enhancing the biocompatibility of scaffolds special as a good skirt in keratoprosthesis designs.  相似文献   

Plant community dynamics in arid lands: the role of desert ants     

Mohsen SHARAFATMANDRAD  Azam KHOSRAVI MASHIZI 《干旱区科学》2021,13(3):303-316

Ants (Formicidae, Hymenoptera) play an important role in seed bank, seedling establishment andplant composition of arid ecosystems. Thus, knowing plant-ant interaction provides useful information formanagers to design restoration and conservation plans. In this study, the roles of desert harvester ants (Messorintermedius and Messor melancholicus) and scavenger ants (Cataglyphis nodus and Lepisiota semenovi) on plantcommunities were investigated in arid ecosystems of southeastern Iran. Two vegetation types weredistinguished in the study area and the nest density of ant species was determined. Furthermore, plantcomposition and soil seed bank were estimated at different distances from the ant nests. Results showed thatthe density of M. intermedius and M. melancholicus nests was higher in dwarf shrub-shrub vegetation type and thedensity of C. nodus and L. semenovi nests was higher in dwarf shrub vegetation type. The harvester and scavengerants had enhanced the seed bank to 55% and 70%, respectively. Therefore, the role of scavenger ants on theplant communities' seed bank was greater than that of harvester ants. Although the scavenger ants were moreinfluential on the annuals and the invasive plant species, the radius impact of the harvester ants on theperennials was greater, i.e., a positive interaction existed between the perennial plants and the harvester ants. C.nodus and L. semenovi played an important role in enhancing the ecosystem's potential for restoration throughestablishment of pioneer species in early stage of succession. The activity of M. intermedius is crucial for thedevelopment and maintenance of climax plant communities in arid ecosystems through assisting the plantspecies' establishment in late stage of succession. It is essential to preserve the diversity of these key ant speciesfor the maintenance and sustainability of shrubs in arid ecosystems.  相似文献